Microbiological evaluation of Escherichia coli in cuts of beef (ground) sold in the Municipalities of Valença and Vassouras, RJ

Detalhes bibliográficos
Autor(a) principal: Lima, Eduardo de Assis
Data de Publicação: 2020
Outros Autores: Lima, Ana Carla Pinheiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/4736
Resumo: The importance of beef (ground) in human food and the concern to offer an innocuous food that is incapable of transmitting diseases led to the development of this study, which aimed to evaluate the microbiological quality, the antimicrobial sensitivity profile and the hygienic-sanitary conditions in samples of beef (ground), sold in the Municipalities of Valença and Vassouras, RJ. Samples were carried out in 20 commercial establishments, which offer ground meat under conditions of consumption and analyzed according to the coliform quantification technique in MPN/g. The antimicrobial profile of Escherichia coli isolates was submitted to susceptibility tests to tetracycline (30μg), ampicillin (10μg), cephalothin (30μg), gentamicin (10μg) and cefotaxime (30μg), using the disk-diffusion method. The assessment of hygienic-sanitary conditions, on the other hand, was based on pre-established observation criteria in a model questionnaire. The total coliform indices were greater than 240 MPN/g in 90% of the analyzed samples and 55% of the samples had indices greater than 240 MPN/g for thermotolerant coliforms, and the Municipality of Valença revealed 40% of contamination within the sample universe. The evaluation of the antimicrobial profile for the antibiotic cefotaxime showed a greater spectrum of action for strains of Escherichia coli isolated from the samples, while the highest index of resistance was observed for the antibiotic cephalothin. The evaluation of hygienic-sanitary conditions was considered unsatisfactory in relation to the handling of meat, which makes this type of food a potential cause of food poisoning.
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spelling Microbiological evaluation of Escherichia coli in cuts of beef (ground) sold in the Municipalities of Valença and Vassouras, RJEvaluación microbiológica de Escherichia colien cortes de carne (molida) vendidos en los Municipios de Valença y Vassouras, RJAvaliação microbiológica de Escherichia coli em cortes de carne bovina (moída) comercializada nos Municípios de Valença e Vassouras, RJAntimicrobial profileBeefEscherichia coliFood poisoningTotal and thermotolerant coliforms.Carne bovinaColiformes totais e termotolerantesEscherichia coliIntoxicações alimentaresPerfil antimicrobiano.Carne de resColiformes totales y termotolerantesEscherichia coliintoxicación alimentariaPerfil antimicrobiano.The importance of beef (ground) in human food and the concern to offer an innocuous food that is incapable of transmitting diseases led to the development of this study, which aimed to evaluate the microbiological quality, the antimicrobial sensitivity profile and the hygienic-sanitary conditions in samples of beef (ground), sold in the Municipalities of Valença and Vassouras, RJ. Samples were carried out in 20 commercial establishments, which offer ground meat under conditions of consumption and analyzed according to the coliform quantification technique in MPN/g. The antimicrobial profile of Escherichia coli isolates was submitted to susceptibility tests to tetracycline (30μg), ampicillin (10μg), cephalothin (30μg), gentamicin (10μg) and cefotaxime (30μg), using the disk-diffusion method. The assessment of hygienic-sanitary conditions, on the other hand, was based on pre-established observation criteria in a model questionnaire. The total coliform indices were greater than 240 MPN/g in 90% of the analyzed samples and 55% of the samples had indices greater than 240 MPN/g for thermotolerant coliforms, and the Municipality of Valença revealed 40% of contamination within the sample universe. The evaluation of the antimicrobial profile for the antibiotic cefotaxime showed a greater spectrum of action for strains of Escherichia coli isolated from the samples, while the highest index of resistance was observed for the antibiotic cephalothin. The evaluation of hygienic-sanitary conditions was considered unsatisfactory in relation to the handling of meat, which makes this type of food a potential cause of food poisoning.La importancia de la carne de res (molida) en la alimentación humana y la preocupación por oferecer  un alimento inocuo que no sea capaz de transmitir enfermedades condujo al desarrollo de este trabajo, cuyo objetivo era evaluar la calidad microbiológica, el perfil de sensibilidad antimicrobiana y las condiciones higiénico-sanitarias en muestras de carne de res (molida), vendida en los municipios de Valença y Vassouras, RJ. Las muestras se llevaron a cabo en 20 establecimientos comerciales, que ofrecen carne molida en condiciones de consumo y se analizaron de acuerdo con la técnica de cuantificación de coliformes en NMP/g. El perfil antimicrobiano de los aislados de Escherichia coli se sometió a pruebas de susceptibilidad a tetraciclina (30 μg), ampicilina (10 μg), cefalotina (30 μg), gentamicina (10 μg) y cefotaxima (30 μg), utilizando el método de difusión en disco. La evaluación de las condiciones higiénico-sanitarias, por otro lado, se basó en criterios de observación preestablecidos en un cuestionario modelo. Los índices de coliformes totales fueron superiores a 240 NMP/g en el 90% de las muestras analizadas y el 55% de las muestras tenían índices superiores a 240 NMP/g para coliformes termotolerantes, y el Municipio de Valença reveló el 40% de la contaminación dentro del universo de la muestra. . La evaluación del perfil antimicrobiano para el antibiótico Cefotaxima reveló un mayor espectro de acción para las cepas de Echerichia coli aisladas de las muestras, mientras que se observó el mayor índice de resistencia para el antibiótico cefalotina. La evaluación de las condiciones higiénico-sanitarias se consideró insatisfactoria en relación con el manejo de la carne, lo que hace que este tipo de alimentos sea una posible causa de intoxicación alimentaria.A importância da carne bovina (moída) na alimentação humana e a preocupação de oferecer um alimento inócuo e incapaz de transmitir doenças induziram ao desenvolvimento deste trabalho, que teve por objetivo avaliara qualidade microbiológica, o perfil de sensibilidade antimicrobiano e as condições higiênico-sanitárias em amostras de carne bovina (moída), comercializadas nos Municípios de Valença e Vassouras, RJ. Foram realizadas amostragens em 20 estabelecimentos comerciais, que oferecem carne moída em condições de consumo e analisados segundo a técnica de quantificação de coliformes em NMP/g. O perfil antimicrobiano dos isolados de Escherichia coli foi submetido aos testes de suscetibilidade à tetraciclina (30μg), ampicilina (10μg), cefalotina (30μg), gentamicina (10μg) e cefotaxima (30μg), através do método de disco-difusão. Já, a avaliação das condições higiênico-sanitárias, deu-se a partir de critérios de observações pré-estabelecidos em questionário modelo. Os índices de coliformes totais foram superiores a 240 NMP/g em 90% das amostras analisadas e 55% das amostras apresentaram índices superiores a 240 NMP/g para coliformes termotolerantes, sendo que o Município de Valença revelou 40% de contaminação dentro do universo amostral. A avaliação do perfil antimicrobiano para o antibiótico cefotaxima revelou maior espectro de atuação para as estirpes de Escherichia coli isoladas das amostras, enquanto o maior índice de resistência foi observado para o antibiótico cefalotina. A avaliação das condições higiênico-sanitárias foi considerada insatisfatória em relação à manipulação da carne, o que torna esse tipo de alimento um potencial causador de toxinfecções alimentares.Research, Society and Development2020-06-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/473610.33448/rsd-v9i7.4736Research, Society and Development; Vol. 9 No. 7; e838974736Research, Society and Development; Vol. 9 Núm. 7; e838974736Research, Society and Development; v. 9 n. 7; e8389747362525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/4736/4263Copyright (c) 2020 Eduardo Assis Lima, Ana Carla Pinheiro Limainfo:eu-repo/semantics/openAccessLima, Eduardo de AssisLima, Ana Carla Pinheiro2020-08-20T18:05:03Zoai:ojs.pkp.sfu.ca:article/4736Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:28:29.259975Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Microbiological evaluation of Escherichia coli in cuts of beef (ground) sold in the Municipalities of Valença and Vassouras, RJ
Evaluación microbiológica de Escherichia colien cortes de carne (molida) vendidos en los Municipios de Valença y Vassouras, RJ
Avaliação microbiológica de Escherichia coli em cortes de carne bovina (moída) comercializada nos Municípios de Valença e Vassouras, RJ
title Microbiological evaluation of Escherichia coli in cuts of beef (ground) sold in the Municipalities of Valença and Vassouras, RJ
spellingShingle Microbiological evaluation of Escherichia coli in cuts of beef (ground) sold in the Municipalities of Valença and Vassouras, RJ
Lima, Eduardo de Assis
Antimicrobial profile
Beef
Escherichia coli
Food poisoning
Total and thermotolerant coliforms.
Carne bovina
Coliformes totais e termotolerantes
Escherichia coli
Intoxicações alimentares
Perfil antimicrobiano.
Carne de res
Coliformes totales y termotolerantes
Escherichia coli
intoxicación alimentaria
Perfil antimicrobiano.
title_short Microbiological evaluation of Escherichia coli in cuts of beef (ground) sold in the Municipalities of Valença and Vassouras, RJ
title_full Microbiological evaluation of Escherichia coli in cuts of beef (ground) sold in the Municipalities of Valença and Vassouras, RJ
title_fullStr Microbiological evaluation of Escherichia coli in cuts of beef (ground) sold in the Municipalities of Valença and Vassouras, RJ
title_full_unstemmed Microbiological evaluation of Escherichia coli in cuts of beef (ground) sold in the Municipalities of Valença and Vassouras, RJ
title_sort Microbiological evaluation of Escherichia coli in cuts of beef (ground) sold in the Municipalities of Valença and Vassouras, RJ
author Lima, Eduardo de Assis
author_facet Lima, Eduardo de Assis
Lima, Ana Carla Pinheiro
author_role author
author2 Lima, Ana Carla Pinheiro
author2_role author
dc.contributor.author.fl_str_mv Lima, Eduardo de Assis
Lima, Ana Carla Pinheiro
dc.subject.por.fl_str_mv Antimicrobial profile
Beef
Escherichia coli
Food poisoning
Total and thermotolerant coliforms.
Carne bovina
Coliformes totais e termotolerantes
Escherichia coli
Intoxicações alimentares
Perfil antimicrobiano.
Carne de res
Coliformes totales y termotolerantes
Escherichia coli
intoxicación alimentaria
Perfil antimicrobiano.
topic Antimicrobial profile
Beef
Escherichia coli
Food poisoning
Total and thermotolerant coliforms.
Carne bovina
Coliformes totais e termotolerantes
Escherichia coli
Intoxicações alimentares
Perfil antimicrobiano.
Carne de res
Coliformes totales y termotolerantes
Escherichia coli
intoxicación alimentaria
Perfil antimicrobiano.
description The importance of beef (ground) in human food and the concern to offer an innocuous food that is incapable of transmitting diseases led to the development of this study, which aimed to evaluate the microbiological quality, the antimicrobial sensitivity profile and the hygienic-sanitary conditions in samples of beef (ground), sold in the Municipalities of Valença and Vassouras, RJ. Samples were carried out in 20 commercial establishments, which offer ground meat under conditions of consumption and analyzed according to the coliform quantification technique in MPN/g. The antimicrobial profile of Escherichia coli isolates was submitted to susceptibility tests to tetracycline (30μg), ampicillin (10μg), cephalothin (30μg), gentamicin (10μg) and cefotaxime (30μg), using the disk-diffusion method. The assessment of hygienic-sanitary conditions, on the other hand, was based on pre-established observation criteria in a model questionnaire. The total coliform indices were greater than 240 MPN/g in 90% of the analyzed samples and 55% of the samples had indices greater than 240 MPN/g for thermotolerant coliforms, and the Municipality of Valença revealed 40% of contamination within the sample universe. The evaluation of the antimicrobial profile for the antibiotic cefotaxime showed a greater spectrum of action for strains of Escherichia coli isolated from the samples, while the highest index of resistance was observed for the antibiotic cephalothin. The evaluation of hygienic-sanitary conditions was considered unsatisfactory in relation to the handling of meat, which makes this type of food a potential cause of food poisoning.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-17
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/4736
10.33448/rsd-v9i7.4736
url https://rsdjournal.org/index.php/rsd/article/view/4736
identifier_str_mv 10.33448/rsd-v9i7.4736
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/4736/4263
dc.rights.driver.fl_str_mv Copyright (c) 2020 Eduardo Assis Lima, Ana Carla Pinheiro Lima
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Eduardo Assis Lima, Ana Carla Pinheiro Lima
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 7; e838974736
Research, Society and Development; Vol. 9 Núm. 7; e838974736
Research, Society and Development; v. 9 n. 7; e838974736
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052650691231744

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