Citric acid production by Aspergillus spp. through submerged fermentation using different production mediums containing agroindustrial residues

Detalhes bibliográficos
Autor(a) principal: Medeiros, Alexandre D'Lamare Maia de
Data de Publicação: 2022
Outros Autores: Medeiros, Thiago Pettrus Maia de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/28839
Resumo: The production of secondary metabolites of high added value by microorganisms has been extensively studied, mainly in the formulation of medium containing agro-industrial residues. Citric acid is a tricarboxylic organic acid obtained through submerged fermentation processes. The Aspergillus genus is considered an excellent producer of bioactive substances of industrial interest. This study aims to determine the rate of citric acid production using four strains of the microorganism (UCP 1099, 1356, 1357, and 1463) in alternative culture mediums containing citrus pomace (pineapple, orange and lemon) from the food industries in the state of Pernambuco, and to compare the results with the production obtained with the standard production medium using computational statistical tools. Assays were carried out with four samples of Aspergillus isolated from Caatinga in three conventional mediums, for 144 h, 37°C, and 180 rpm. The pH variation, sugar consumption, and citric acid production were analyzed. The results showed that the medium-called two e and the sample UCP1357, presented a citric acid production of 7.22g.L-1. After selecting the medium and the best producer microorganisms, production tests were carried out with a medium formulated with citrus fruit residues. The results revealed that all the residues produced citric acid, with the lemon peel being the best of the residues tested (6.91 g.L-1). It appears that there is a possibility of using residues in the formulation of means of production, thus contributing to the reuse of the present nutrients and the reduction of the environmental impact.
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spelling Citric acid production by Aspergillus spp. through submerged fermentation using different production mediums containing agroindustrial residuesProducción de ácido cítrico por Aspergillus spp. mediante fermentación sumergida utilizando diferentes medios de producción conteniendo residuos agroindustrialesProdução de ácido cítrico por Aspergillus spp. por fermentação submersa utilizando diferentes meios de produção contendo resíduos agroindustriaisMedio alternativoPotencial biotecnológicoHongos filamentososEnseñanza en la salud.Meio alternativoPotencial biotecnológicoFungos filamentososEnsino em saúde.Alternative mediumBiotechnological potentialFilamentous fungiHealth teaching.The production of secondary metabolites of high added value by microorganisms has been extensively studied, mainly in the formulation of medium containing agro-industrial residues. Citric acid is a tricarboxylic organic acid obtained through submerged fermentation processes. The Aspergillus genus is considered an excellent producer of bioactive substances of industrial interest. This study aims to determine the rate of citric acid production using four strains of the microorganism (UCP 1099, 1356, 1357, and 1463) in alternative culture mediums containing citrus pomace (pineapple, orange and lemon) from the food industries in the state of Pernambuco, and to compare the results with the production obtained with the standard production medium using computational statistical tools. Assays were carried out with four samples of Aspergillus isolated from Caatinga in three conventional mediums, for 144 h, 37°C, and 180 rpm. The pH variation, sugar consumption, and citric acid production were analyzed. The results showed that the medium-called two e and the sample UCP1357, presented a citric acid production of 7.22g.L-1. After selecting the medium and the best producer microorganisms, production tests were carried out with a medium formulated with citrus fruit residues. The results revealed that all the residues produced citric acid, with the lemon peel being the best of the residues tested (6.91 g.L-1). It appears that there is a possibility of using residues in the formulation of means of production, thus contributing to the reuse of the present nutrients and the reduction of the environmental impact.La producción de metabolitos secundarios de alto valor agregado por parte de microorganismos ha sido ampliamente estudiada, principalmente en la formulación de medios que contienen residuos agroindustriales. El ácido cítrico es un ácido orgánico tricarboxílico obtenido mediante procesos de fermentación sumergida. El género Aspergillus es considerado un excelente productor de sustancias bioactivas de interés industrial. Este estudio tiene como objetivo determinar la tasa de producción de ácido cítrico utilizando cuatro cepas del microorganismo (UCP 1099, 1356, 1357 y 1463) en medios de cultivo alternativos que contienen orujo de cítricos (piña, naranja y limón) de las industrias alimentarias del estado de Pernambuco, y comparar los resultados con la producción obtenida con el medio de producción estándar utilizando herramientas estadísticas computacionales. Los ensayos se realizaron con cuatro muestras de Aspergillus aislado de Caatinga en tres medios convencionales, durante 144 h, 37 °C y 180 rpm. Se analizó la variación del pH, el consumo de azúcar y la producción de ácido cítrico. Los resultados mostraron que el medio denominado dos e y la muestra UCP1357, presentaron una producción de ácido cítrico de 7.22g.L-1. Tras seleccionar el medio y los mejores microorganismos productores, se realizaron pruebas de producción con un medio formulado con residuos de cítricos. Los resultados revelaron que todos los residuos produjeron ácido cítrico, siendo la cáscara de limón el mejor de los residuos probados (6.91 g.L-1). Parece que existe la posibilidad de utilizar residuos en la formulación de medios de producción, contribuyendo así a la reutilización de los nutrientes presentes ya la reducción del impacto ambiental.A produção de metabólitos secundários de alto valor agregado por microrganismos tem sido amplamente estudado, principalmente na formulação de meios contendo resíduos agroindustriais. O ácido cítrico é um ácido orgânico tricarboxílico obtido através de processos de fermentação submersa. O gênero Aspergillus é considerado um excelente produtor de substâncias bioativas de interesse industrial. Este estudo tem como objetivo determinar a taxa de produção de ácido cítrico utilizando quatro cepas do microrganismo (UCP 1099, 1356, 1357 e 1463) em meios de cultura alternativos contendo resíduos de frutas (abacaxi, laranja e limão) provenientes de indústrias alimentícias do estado de Pernambuco, e comparar os resultados com a produção obtida com o meio de produção padrão utilizando ferramentas estatísticas computacionais. Os ensaios foram realizados com quatro amostras de Aspergillus isoladas da Caatinga em três meios convencionais, por 144 h, 37°C e 180 rpm. A variação de pH, consumo de açúcar e produção de ácido cítrico foram analisados. Os resultados mostraram que o meio denominado “dois” e a amostra UCP1357, apresentaram uma produção de ácido cítrico de 7,22 g.L-1. Após a seleção do meio e dos microrganismos melhores produtores, foram realizados testes de produção com um meio formulado com os resíduos de frutas cítricas. Os resultados revelaram que todos os resíduos produziram ácido cítrico, sendo a casca de limão o melhor dos resíduos testados (6,91 g.L-1). Verifica-se que existe a possibilidade de utilização de resíduos na formulação de meios de produção, contribuindo assim para o reaproveitamento dos nutrientes presentes e a redução do impacto ambiental.Research, Society and Development2022-04-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2883910.33448/rsd-v11i6.28839Research, Society and Development; Vol. 11 No. 6; e8011628839Research, Society and Development; Vol. 11 Núm. 6; e8011628839Research, Society and Development; v. 11 n. 6; e80116288392525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/28839/24995Copyright (c) 2022 Alexandre D'Lamare Maia de Medeiros; Thiago Pettrus Maia de Medeiroshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMedeiros, Alexandre D'Lamare Maia deMedeiros, Thiago Pettrus Maia de2022-05-13T18:04:10Zoai:ojs.pkp.sfu.ca:article/28839Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:46:06.537285Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Citric acid production by Aspergillus spp. through submerged fermentation using different production mediums containing agroindustrial residues
Producción de ácido cítrico por Aspergillus spp. mediante fermentación sumergida utilizando diferentes medios de producción conteniendo residuos agroindustriales
Produção de ácido cítrico por Aspergillus spp. por fermentação submersa utilizando diferentes meios de produção contendo resíduos agroindustriais
title Citric acid production by Aspergillus spp. through submerged fermentation using different production mediums containing agroindustrial residues
spellingShingle Citric acid production by Aspergillus spp. through submerged fermentation using different production mediums containing agroindustrial residues
Medeiros, Alexandre D'Lamare Maia de
Medio alternativo
Potencial biotecnológico
Hongos filamentosos
Enseñanza en la salud.
Meio alternativo
Potencial biotecnológico
Fungos filamentosos
Ensino em saúde.
Alternative medium
Biotechnological potential
Filamentous fungi
Health teaching.
title_short Citric acid production by Aspergillus spp. through submerged fermentation using different production mediums containing agroindustrial residues
title_full Citric acid production by Aspergillus spp. through submerged fermentation using different production mediums containing agroindustrial residues
title_fullStr Citric acid production by Aspergillus spp. through submerged fermentation using different production mediums containing agroindustrial residues
title_full_unstemmed Citric acid production by Aspergillus spp. through submerged fermentation using different production mediums containing agroindustrial residues
title_sort Citric acid production by Aspergillus spp. through submerged fermentation using different production mediums containing agroindustrial residues
author Medeiros, Alexandre D'Lamare Maia de
author_facet Medeiros, Alexandre D'Lamare Maia de
Medeiros, Thiago Pettrus Maia de
author_role author
author2 Medeiros, Thiago Pettrus Maia de
author2_role author
dc.contributor.author.fl_str_mv Medeiros, Alexandre D'Lamare Maia de
Medeiros, Thiago Pettrus Maia de
dc.subject.por.fl_str_mv Medio alternativo
Potencial biotecnológico
Hongos filamentosos
Enseñanza en la salud.
Meio alternativo
Potencial biotecnológico
Fungos filamentosos
Ensino em saúde.
Alternative medium
Biotechnological potential
Filamentous fungi
Health teaching.
topic Medio alternativo
Potencial biotecnológico
Hongos filamentosos
Enseñanza en la salud.
Meio alternativo
Potencial biotecnológico
Fungos filamentosos
Ensino em saúde.
Alternative medium
Biotechnological potential
Filamentous fungi
Health teaching.
description The production of secondary metabolites of high added value by microorganisms has been extensively studied, mainly in the formulation of medium containing agro-industrial residues. Citric acid is a tricarboxylic organic acid obtained through submerged fermentation processes. The Aspergillus genus is considered an excellent producer of bioactive substances of industrial interest. This study aims to determine the rate of citric acid production using four strains of the microorganism (UCP 1099, 1356, 1357, and 1463) in alternative culture mediums containing citrus pomace (pineapple, orange and lemon) from the food industries in the state of Pernambuco, and to compare the results with the production obtained with the standard production medium using computational statistical tools. Assays were carried out with four samples of Aspergillus isolated from Caatinga in three conventional mediums, for 144 h, 37°C, and 180 rpm. The pH variation, sugar consumption, and citric acid production were analyzed. The results showed that the medium-called two e and the sample UCP1357, presented a citric acid production of 7.22g.L-1. After selecting the medium and the best producer microorganisms, production tests were carried out with a medium formulated with citrus fruit residues. The results revealed that all the residues produced citric acid, with the lemon peel being the best of the residues tested (6.91 g.L-1). It appears that there is a possibility of using residues in the formulation of means of production, thus contributing to the reuse of the present nutrients and the reduction of the environmental impact.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/28839
10.33448/rsd-v11i6.28839
url https://rsdjournal.org/index.php/rsd/article/view/28839
identifier_str_mv 10.33448/rsd-v11i6.28839
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/28839/24995
dc.rights.driver.fl_str_mv Copyright (c) 2022 Alexandre D'Lamare Maia de Medeiros; Thiago Pettrus Maia de Medeiros
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Alexandre D'Lamare Maia de Medeiros; Thiago Pettrus Maia de Medeiros
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 6; e8011628839
Research, Society and Development; Vol. 11 Núm. 6; e8011628839
Research, Society and Development; v. 11 n. 6; e8011628839
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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