Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/37712 |
Resumo: | The analysis of fucoxanthin (FCX) in algal biomasses presents some limitations, especially regarding the sample preservation after collection until extraction of the pigment. FCX stability was evaluated in biomass samples of one diatom and three brown seaweed species (Phaeophyceae) and their extracts stored at -20ºC and -80ºC, for 60 days. Fucoxanthin was quantified through UHPLC-DAD. The methanolic extracts of the micro/macroalga biomasses stored at -20ºC and -80ºC were more effective in preserving the xanthophyll, comparatively to the algal biomasses. Besides, the chromatographic analysis showed an increase in the FCX’s peak retention time along the sample storage, possibly due to the trans→cis isomerization of the molecule. Such information is relevant for choosing the best protocol for storing and preserving at most FCX, because changes in the geometric conformations of the molecule over storage strongly affects its biological activities. |
id |
UNIFEI_7849c91250ac8766033292862ba795f7 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/37712 |
network_acronym_str |
UNIFEI |
network_name_str |
Research, Society and Development |
repository_id_str |
|
spelling |
Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperaturesMonitoreo de la estabilidad de la xantofila fucoxantina en biomasas de microalgas y algas y extractos almacenados a bajas temperaturasMonitoramento da estabilidade da xantofila fucoxantina em biomassas de microalga e algas marinhas e extratos armazenados a baixas temperaturasPhaeophyceaeFucoxanthin isomersCarotenoidsUHPLCBacillariophyceae.PhaeophyceaeIsómeros de FucoxantinaCarotenoidesUHPLCBacillariophyceae.PhaeophyceaeIsômeros de FucoxantinaCarotenoidesUHPLCBacillariophyceae.The analysis of fucoxanthin (FCX) in algal biomasses presents some limitations, especially regarding the sample preservation after collection until extraction of the pigment. FCX stability was evaluated in biomass samples of one diatom and three brown seaweed species (Phaeophyceae) and their extracts stored at -20ºC and -80ºC, for 60 days. Fucoxanthin was quantified through UHPLC-DAD. The methanolic extracts of the micro/macroalga biomasses stored at -20ºC and -80ºC were more effective in preserving the xanthophyll, comparatively to the algal biomasses. Besides, the chromatographic analysis showed an increase in the FCX’s peak retention time along the sample storage, possibly due to the trans→cis isomerization of the molecule. Such information is relevant for choosing the best protocol for storing and preserving at most FCX, because changes in the geometric conformations of the molecule over storage strongly affects its biological activities.El análisis de fucoxantina (FCX) en biomasas de algas presenta algunas limitaciones, especialmente en lo que se refiere a la conservación de la muestra después de su recolección hasta la extracción del pigmento. La estabilidad de FCX se evaluó en muestras de biomasa y extracto de una diatomea y tres especies de algas marrón (Phaeophyceae) almacenados a -20ºC y -80ºC, durante 60 días. La FCX se cuantificó mediante UHPLC-DAD. Los extractos metanólicos de las biomasas de micro/macroalgas almacenados a -20ºC y -80ºC fueron más efectivos en la conservación de FCX, en comparación a las biomasas de algas. Además, el análisis cromatográfico mostró un aumento del tiempo de retención del pico de FCX a lo largo del almacenamiento de la muestra, posiblemente debido a la isomerización trans→cis de la molécula. Esta información es relevante para elegir el mejor protocolo de almacenamiento y preservacion de FCX, porque los cambios de conformaciones geométricas de la molécula durante el almacenamiento afectam fuertemente sus actividades biológicas.A análise de fucoxantina (FCX) em biomassa de algas apresenta algumas limitações, principalmente quanto à preservação das amostras após a coleta até a extração do pigmento. A estabilidade da FCX foi avaliada em amostras de biomassas e extrato de uma diatomacea e três espécies de macroalgas marrons (Phaeophyceae) armazenadas à -20ºC e -80ºC, por 60 dias. Os conteúdos de FCX nas amostras foram determinados por UHPLC-DAD. Os extratos metanólicos das biomassas de micro/macroalgas armazenadas à -20ºC e -80ºC foram mais eficazes na preservação da xantofila, comparativamente às biomassas algais. A análise cromatográfica mostrou um aumento no tempo de retenção do pico de FCX nas amostras ao longo do armazenamento, possivelmente devido à isomerização trans→cis da molécula. Tal informação é relevante à escolha do melhor protocolo de armazenamento para preservar ao máximo os teores daquele oxicarotenoide, porque alterações nas conformações geométricas da molécula ao longo do armazenamento impactam fortemente nas atividades biológicas da FCX.Research, Society and Development2022-11-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3771210.33448/rsd-v11i15.37712Research, Society and Development; Vol. 11 No. 15; e577111537712Research, Society and Development; Vol. 11 Núm. 15; e577111537712Research, Society and Development; v. 11 n. 15; e5771115377122525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/37712/31305Copyright (c) 2022 Caroline Schmitz; Aline Nunes; Cláudia Marlene Bauer ; José Bonomi-Barufi; Marcelo Maraschinhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSchmitz, CarolineNunes, AlineBauer , Cláudia Marlene Bonomi-Barufi, JoséMaraschin, Marcelo2022-11-27T19:56:23Zoai:ojs.pkp.sfu.ca:article/37712Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:51:46.687901Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures Monitoreo de la estabilidad de la xantofila fucoxantina en biomasas de microalgas y algas y extractos almacenados a bajas temperaturas Monitoramento da estabilidade da xantofila fucoxantina em biomassas de microalga e algas marinhas e extratos armazenados a baixas temperaturas |
title |
Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures |
spellingShingle |
Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures Schmitz, Caroline Phaeophyceae Fucoxanthin isomers Carotenoids UHPLC Bacillariophyceae. Phaeophyceae Isómeros de Fucoxantina Carotenoides UHPLC Bacillariophyceae. Phaeophyceae Isômeros de Fucoxantina Carotenoides UHPLC Bacillariophyceae. |
title_short |
Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures |
title_full |
Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures |
title_fullStr |
Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures |
title_full_unstemmed |
Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures |
title_sort |
Monitoring the stability of the xanthophyll fucoxanthin in microalga and seaweed biomasses, and extracts stored at low temperatures |
author |
Schmitz, Caroline |
author_facet |
Schmitz, Caroline Nunes, Aline Bauer , Cláudia Marlene Bonomi-Barufi, José Maraschin, Marcelo |
author_role |
author |
author2 |
Nunes, Aline Bauer , Cláudia Marlene Bonomi-Barufi, José Maraschin, Marcelo |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Schmitz, Caroline Nunes, Aline Bauer , Cláudia Marlene Bonomi-Barufi, José Maraschin, Marcelo |
dc.subject.por.fl_str_mv |
Phaeophyceae Fucoxanthin isomers Carotenoids UHPLC Bacillariophyceae. Phaeophyceae Isómeros de Fucoxantina Carotenoides UHPLC Bacillariophyceae. Phaeophyceae Isômeros de Fucoxantina Carotenoides UHPLC Bacillariophyceae. |
topic |
Phaeophyceae Fucoxanthin isomers Carotenoids UHPLC Bacillariophyceae. Phaeophyceae Isómeros de Fucoxantina Carotenoides UHPLC Bacillariophyceae. Phaeophyceae Isômeros de Fucoxantina Carotenoides UHPLC Bacillariophyceae. |
description |
The analysis of fucoxanthin (FCX) in algal biomasses presents some limitations, especially regarding the sample preservation after collection until extraction of the pigment. FCX stability was evaluated in biomass samples of one diatom and three brown seaweed species (Phaeophyceae) and their extracts stored at -20ºC and -80ºC, for 60 days. Fucoxanthin was quantified through UHPLC-DAD. The methanolic extracts of the micro/macroalga biomasses stored at -20ºC and -80ºC were more effective in preserving the xanthophyll, comparatively to the algal biomasses. Besides, the chromatographic analysis showed an increase in the FCX’s peak retention time along the sample storage, possibly due to the trans→cis isomerization of the molecule. Such information is relevant for choosing the best protocol for storing and preserving at most FCX, because changes in the geometric conformations of the molecule over storage strongly affects its biological activities. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/37712 10.33448/rsd-v11i15.37712 |
url |
https://rsdjournal.org/index.php/rsd/article/view/37712 |
identifier_str_mv |
10.33448/rsd-v11i15.37712 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/37712/31305 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 15; e577111537712 Research, Society and Development; Vol. 11 Núm. 15; e577111537712 Research, Society and Development; v. 11 n. 15; e577111537712 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052730012860416 |