Pfaffia glomerata: Gallic acid and phenolic compounds determination
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/38706 |
Resumo: | Pfaffia glomerata, known as ginseng-brasileiro, is a root widely used in folk medicine, being effective against inflammation, rheumatism, and fatigue, in addition to present aphrodisiacs and antidiabetics properties. Among the classes of substances reported for the specie, the phenolic acids, in special gallic acid, has shown positive activity to learning and memory, having a considerable effect against neurotoxic and pro-oxidant substances. Therefore, the article’s objective was to quantify gallic acid in the chloroform and ethyl acetate fractions from P. glomerata. Thus, the aerial parts of ginseng were collected, dried and subjected to extraction with ethanol followed by fractionation with organic solvents in different polarities, obtaining the hexanic, dichloromethane (CHCl3) and ethyl acetate (AcOEt) fractions. Then, gallic acid was identified by TLC and quantified by HPLC in the ethyl acetate fraction. The total phenolic content was determined in the chloroform and ethyl acetate fractions, presenting values of 70,3 mg/g and 118,6 mg/g, respectively. The gallic acid content determined by HPLC in the AcOEt fraction was 904,15 µg/g, while in the CHCl3 fraction it was not possible to quantify. So, the species can be a source of phenolic compounds for possible applications in the pharmaceutical area. |
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Pfaffia glomerata: Gallic acid and phenolic compounds determinationPfaffia glomerata: determinación de compuestos fenólicos y ácido gálicoPfaffia glomerata: determinação de compostos fenólicos e ácido gálicoHPLCGinsengSpectrophotometry.GinsengHPLCEspectrofotometría.GinsengCLAEEspectrofotometria.Pfaffia glomerata, known as ginseng-brasileiro, is a root widely used in folk medicine, being effective against inflammation, rheumatism, and fatigue, in addition to present aphrodisiacs and antidiabetics properties. Among the classes of substances reported for the specie, the phenolic acids, in special gallic acid, has shown positive activity to learning and memory, having a considerable effect against neurotoxic and pro-oxidant substances. Therefore, the article’s objective was to quantify gallic acid in the chloroform and ethyl acetate fractions from P. glomerata. Thus, the aerial parts of ginseng were collected, dried and subjected to extraction with ethanol followed by fractionation with organic solvents in different polarities, obtaining the hexanic, dichloromethane (CHCl3) and ethyl acetate (AcOEt) fractions. Then, gallic acid was identified by TLC and quantified by HPLC in the ethyl acetate fraction. The total phenolic content was determined in the chloroform and ethyl acetate fractions, presenting values of 70,3 mg/g and 118,6 mg/g, respectively. The gallic acid content determined by HPLC in the AcOEt fraction was 904,15 µg/g, while in the CHCl3 fraction it was not possible to quantify. So, the species can be a source of phenolic compounds for possible applications in the pharmaceutical area.Pffafia glomerata, conocido como ginseng brasileño, es una raíz ampliamente utilizada en la medicina popular, siendo eficaz en la lucha contra la inflamación, el reumatismo, fatiga, además de presentar propiedades afrodisíacas y antidiabéticas. Entre las clases de sustancias notificadas para la especie, los ácidos fenólicos, especialmente el ácido lálico, han demostrado actividad positiva en relación con el aprendizaje y la memoria, teniendo un efecto considerable sobre las sustancias neurotóxicos y prooxidantes. Así, el objetivo de este trabajo fue cuantificar el ácido cónico presente en las fracciones de cloroformo y acetato de etilo de P. glomerata. Así, las partes aéreas del ginseng fueron recolectadas, secadas y sometidas a extracción de etanol seguido de fraccionamiento por solventes orgánicos con diferentes polaridades, obteniéndose las fracciones de hexaénica, diclorometania (CHCl3) y acetato de etilo (AcOEt). A continuación, el ácido sálico fue identificado por CCD y cuantificado en HPLC en la fracción de acetato de etilo. El contenido fenólico total se determinó en las fracciones de cloroformo y acetato de etilo, presentando valores de 70,3 mg/g y 118,6 mg/g, respectivamente. El contenido de ácido maníaco determinado por HPLC en la fracción AcOEt fue de 904,15 μg/g, mientras que en la fracción CHCl3 no fue possible cuantificar. Así, la especie puede ser una fuente de compuestos fenólicos para posibles aplicaciones en el área farmacéutica.A Pffafia glomerata, conhecida como ginseng brasileiro, é uma raiz amplamente utilizada na medicina popular, sendo eficaz no combate a inflamações, reumatismo, fadiga, além de apresentar propriedades afrodisíacas e antidiabéticas. Dentre as classes de substâncias relatadas para a espécie, os ácidos fenólicos, em especial o ácido gálico, têm demonstrado atividade positiva em relação ao aprendizado e memória, desempenhando efeito considerável frente a substâncias neurotóxicas e pró-oxidantes. Assim, o objetivo do trabalho foi quantificar o ácido gálico presente nas frações clorofórmica e acetato de etila de P. glomerata. Dessa forma, as partes aéreas do ginseng foram coletadas, secas e submetidas a extração com etanol seguida de fracionamento por solventes orgânicos com diferentes polaridades, obtendo as frações hexânica, diclorometânica (CHCl3) e acetato de etila (AcOEt). Na sequência, o ácido gálico foi identificado por CCD e quantificado em CLAE na fração acetato de etila. O teor de fenólicos totais foi determinado nas frações clorofórmica e acetato de etila, apresentando valores de 70,3 mg/g e 118,6 mg/g, respectivamente. O teor de ácido gálico determinado por CLAE na fração AcOEt foi de 904,15 µg/g, já na fração CHCl3 não foi possível quantificar. Desta forma, a espécie pode ser uma fonte de compostos fenólicos para possíveis aplicações na área farmacêutica.Research, Society and Development2022-12-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3870610.33448/rsd-v11i17.38706Research, Society and Development; Vol. 11 No. 17; e37111738706Research, Society and Development; Vol. 11 Núm. 17; e37111738706Research, Society and Development; v. 11 n. 17; e371117387062525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/38706/32038Copyright (c) 2022 Taina Kleinubing da Silva; Ester Hadassa Neves; Andrey Felipe Kummer; Emanuele Steffens; Maik Breguedo; Cárin Fabíola Pensin; Letycia Lopes Ricardo; Kelly Cristina Massarolohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Taina Kleinubing da Neves, Ester HadassaKummer, Andrey FelipeSteffens, Emanuele Breguedo, MaikPensin, Cárin FabíolaRicardo, Letycia LopesMassarolo, Kelly Cristina2022-12-28T13:53:48Zoai:ojs.pkp.sfu.ca:article/38706Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:52:19.140075Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Pfaffia glomerata: Gallic acid and phenolic compounds determination Pfaffia glomerata: determinación de compuestos fenólicos y ácido gálico Pfaffia glomerata: determinação de compostos fenólicos e ácido gálico |
title |
Pfaffia glomerata: Gallic acid and phenolic compounds determination |
spellingShingle |
Pfaffia glomerata: Gallic acid and phenolic compounds determination Silva, Taina Kleinubing da HPLC Ginseng Spectrophotometry. Ginseng HPLC Espectrofotometría. Ginseng CLAE Espectrofotometria. |
title_short |
Pfaffia glomerata: Gallic acid and phenolic compounds determination |
title_full |
Pfaffia glomerata: Gallic acid and phenolic compounds determination |
title_fullStr |
Pfaffia glomerata: Gallic acid and phenolic compounds determination |
title_full_unstemmed |
Pfaffia glomerata: Gallic acid and phenolic compounds determination |
title_sort |
Pfaffia glomerata: Gallic acid and phenolic compounds determination |
author |
Silva, Taina Kleinubing da |
author_facet |
Silva, Taina Kleinubing da Neves, Ester Hadassa Kummer, Andrey Felipe Steffens, Emanuele Breguedo, Maik Pensin, Cárin Fabíola Ricardo, Letycia Lopes Massarolo, Kelly Cristina |
author_role |
author |
author2 |
Neves, Ester Hadassa Kummer, Andrey Felipe Steffens, Emanuele Breguedo, Maik Pensin, Cárin Fabíola Ricardo, Letycia Lopes Massarolo, Kelly Cristina |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Taina Kleinubing da Neves, Ester Hadassa Kummer, Andrey Felipe Steffens, Emanuele Breguedo, Maik Pensin, Cárin Fabíola Ricardo, Letycia Lopes Massarolo, Kelly Cristina |
dc.subject.por.fl_str_mv |
HPLC Ginseng Spectrophotometry. Ginseng HPLC Espectrofotometría. Ginseng CLAE Espectrofotometria. |
topic |
HPLC Ginseng Spectrophotometry. Ginseng HPLC Espectrofotometría. Ginseng CLAE Espectrofotometria. |
description |
Pfaffia glomerata, known as ginseng-brasileiro, is a root widely used in folk medicine, being effective against inflammation, rheumatism, and fatigue, in addition to present aphrodisiacs and antidiabetics properties. Among the classes of substances reported for the specie, the phenolic acids, in special gallic acid, has shown positive activity to learning and memory, having a considerable effect against neurotoxic and pro-oxidant substances. Therefore, the article’s objective was to quantify gallic acid in the chloroform and ethyl acetate fractions from P. glomerata. Thus, the aerial parts of ginseng were collected, dried and subjected to extraction with ethanol followed by fractionation with organic solvents in different polarities, obtaining the hexanic, dichloromethane (CHCl3) and ethyl acetate (AcOEt) fractions. Then, gallic acid was identified by TLC and quantified by HPLC in the ethyl acetate fraction. The total phenolic content was determined in the chloroform and ethyl acetate fractions, presenting values of 70,3 mg/g and 118,6 mg/g, respectively. The gallic acid content determined by HPLC in the AcOEt fraction was 904,15 µg/g, while in the CHCl3 fraction it was not possible to quantify. So, the species can be a source of phenolic compounds for possible applications in the pharmaceutical area. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/38706 10.33448/rsd-v11i17.38706 |
url |
https://rsdjournal.org/index.php/rsd/article/view/38706 |
identifier_str_mv |
10.33448/rsd-v11i17.38706 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/38706/32038 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 17; e37111738706 Research, Society and Development; Vol. 11 Núm. 17; e37111738706 Research, Society and Development; v. 11 n. 17; e37111738706 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052775560904704 |