Toxicity assessment of mastic and pomegranate tinctures using the Artemia salina bioassay
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/13751 |
Resumo: | This work aimed to evaluate the toxicity of the mastic (Myracrodruon urundeuva) and pomegranate (Punica granatum) tinctures through the bioassay with Artemia salina. Different dye concentrations (25, 50, 75, 150, 250, 500, 750 and 1000 μg/mL) were added to the nauplii and the average lethal concentration (CL50) was evaluated using Probit analysis using the StatPlus® software (AnalystSoft, USA), obtaining the upper and lower confidence intervals, after a 24-hour exposure period. The extracts were considered active when the values were < 1000ug/mL. Survival curves and LogRank tests were performed for all extracts at a concentration of 250 μg / mL against negative control (Saline) and positive control (Chlorhexidine), using the GraphPad® Prism software (GraphPad Software, USA). For the mastic, the LC50 was 607.87 ± 38.73, for the pomegranate, 464.98 ± 28.72, both tinctures being considered active. When compared to saline, there was a significant difference in survival curves (p = 0.0206), the same occurred for comparisons with chlorhexidine (p < 0.0001). It can be concluded that mastic and pomegranate tinctures were toxic against Artemia salina in the different concentrations tested. |
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Toxicity assessment of mastic and pomegranate tinctures using the Artemia salina bioassayEvaluación de la toxicidad de las tinturas de masilla y granada mediante el bioensayo de Artemia salinaAvaliação da toxicidade das tinturas de aroeira e de romã através do bioensaio com Artemia salinaPunicaceaeBurseraToxicity Tests.PunicaceaeBurseraPruebas de Toxicidad.PunicaceaeBurseraTestes de Toxicidade.This work aimed to evaluate the toxicity of the mastic (Myracrodruon urundeuva) and pomegranate (Punica granatum) tinctures through the bioassay with Artemia salina. Different dye concentrations (25, 50, 75, 150, 250, 500, 750 and 1000 μg/mL) were added to the nauplii and the average lethal concentration (CL50) was evaluated using Probit analysis using the StatPlus® software (AnalystSoft, USA), obtaining the upper and lower confidence intervals, after a 24-hour exposure period. The extracts were considered active when the values were < 1000ug/mL. Survival curves and LogRank tests were performed for all extracts at a concentration of 250 μg / mL against negative control (Saline) and positive control (Chlorhexidine), using the GraphPad® Prism software (GraphPad Software, USA). For the mastic, the LC50 was 607.87 ± 38.73, for the pomegranate, 464.98 ± 28.72, both tinctures being considered active. When compared to saline, there was a significant difference in survival curves (p = 0.0206), the same occurred for comparisons with chlorhexidine (p < 0.0001). It can be concluded that mastic and pomegranate tinctures were toxic against Artemia salina in the different concentrations tested.Este trabajo tuvo como objetivo evaluar la toxicidad de las tinturas de masilla (Myracrodruon urundeuva) y granada (Punica granatum) a través del bioensayo con Artemia salina. Se agregaron diferentes concentraciones de colorante (25, 50, 75, 150, 250, 500, 750 y 1000 μg/mL) a los nauplios y se evaluó la concentración letal promedio (CL50) mediante análisis Probit utilizando el software StatPlus® (AnalystSoft, EE. UU.), obteniendo los intervalos de confianza superior e inferior, después de un período de exposición de 24 horas. Los extractos se consideraron activos cuando los valores eran <1000ug / mL. Se realizaron curvas de supervivencia y pruebas de LogRank para todos los extractos a una concentración de 250 μg / mL contra control negativo (solución salina) y control positivo (clorhexidina), utilizando el software GraphPad® Prism (GraphPad Software, EE. UU.). Para la masilla, la CL50 fue de 607,87 ± 38,73, para la granada, 464,98 ± 28,72, considerándose los dos tinturas activas. En comparación con la solución salina, hubo una diferencia significativa en las curvas de supervivencia (p = 0,0206), lo mismo ocurrió para las comparaciones con clorhexidina (p < 0,0001). Se puede concluir que las tinturas de masilla y granada resultaron tóxicas contra Artemia salina en las diferentes concentraciones ensayadas.Este trabalho teve como objetivo avaliar a toxicidade das tinturas de aroeira (Myracrodruon urundeuva) e de romã (Punica granatum) por meio do bioensaio com Artemia salina. Diferentes concentrações das tinturas (25, 50, 75, 150, 250, 500, 750 e 1000μg/mL) foram adicionadas aos náuplios e a concentração letal média (CL50) foi avaliada, utilizando a análise de Probit através do software StatPlus® (AnalystSoft, USA), obtendo-se os intervalos de confiança superior e inferior, após um período de exposição de 24 horas. Os extratos foram considerados ativos quando os valores foram < 1000 ug/mL. Curvas de sobrevivência e testes de LogRank foram realizados para todos os extratos na concentração de 250 μg/mL contra controle negativo (Salina) e controle positivo (Clorexidina), utilizando o software GraphPad® Prism (GraphPad Software, USA). Para a aroeira a CL50 foi de 607,87 ± 38,73, já para a romã, de 464,98 ± 28,72, sendo ambas as tinturas consideradas ativas. Quando comparadas à solução salina, houve diferença significativa nas curvas de sobrevivência (p = 0,0206), o mesmo ocorreu para as comparações com a clorexidina (p < 0,0001). Pode-se concluir que as tinturas de aroeira e de romã foram tóxicas contra a Artemia salina nas diferentes concentrações testadas.Research, Society and Development2021-03-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1375110.33448/rsd-v10i3.13751Research, Society and Development; Vol. 10 No. 3; e52010313751Research, Society and Development; Vol. 10 Núm. 3; e52010313751Research, Society and Development; v. 10 n. 3; e520103137512525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/13751/14365Copyright (c) 2021 Ana Cristina Rodrigues Martins; Jane Kenya Nogueira da Costa; Adyson Herbert; Fabrício Rômulo Sampaio Farias; Márcia Rezende; Vitoldo Antonio Kozlowski Junior; Juliana Larocca de Geushttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMartins, Ana Cristina Rodrigues Costa, Jane Kenya Nogueira da Herbert, Adyson Farias, Fabrício Rômulo Sampaio Rezende, MárciaKozlowski Junior, Vitoldo Antonio Geus, Juliana Larocca de 2021-03-28T12:03:35Zoai:ojs.pkp.sfu.ca:article/13751Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:35:01.754338Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Toxicity assessment of mastic and pomegranate tinctures using the Artemia salina bioassay Evaluación de la toxicidad de las tinturas de masilla y granada mediante el bioensayo de Artemia salina Avaliação da toxicidade das tinturas de aroeira e de romã através do bioensaio com Artemia salina |
title |
Toxicity assessment of mastic and pomegranate tinctures using the Artemia salina bioassay |
spellingShingle |
Toxicity assessment of mastic and pomegranate tinctures using the Artemia salina bioassay Martins, Ana Cristina Rodrigues Punicaceae Bursera Toxicity Tests. Punicaceae Bursera Pruebas de Toxicidad. Punicaceae Bursera Testes de Toxicidade. |
title_short |
Toxicity assessment of mastic and pomegranate tinctures using the Artemia salina bioassay |
title_full |
Toxicity assessment of mastic and pomegranate tinctures using the Artemia salina bioassay |
title_fullStr |
Toxicity assessment of mastic and pomegranate tinctures using the Artemia salina bioassay |
title_full_unstemmed |
Toxicity assessment of mastic and pomegranate tinctures using the Artemia salina bioassay |
title_sort |
Toxicity assessment of mastic and pomegranate tinctures using the Artemia salina bioassay |
author |
Martins, Ana Cristina Rodrigues |
author_facet |
Martins, Ana Cristina Rodrigues Costa, Jane Kenya Nogueira da Herbert, Adyson Farias, Fabrício Rômulo Sampaio Rezende, Márcia Kozlowski Junior, Vitoldo Antonio Geus, Juliana Larocca de |
author_role |
author |
author2 |
Costa, Jane Kenya Nogueira da Herbert, Adyson Farias, Fabrício Rômulo Sampaio Rezende, Márcia Kozlowski Junior, Vitoldo Antonio Geus, Juliana Larocca de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Martins, Ana Cristina Rodrigues Costa, Jane Kenya Nogueira da Herbert, Adyson Farias, Fabrício Rômulo Sampaio Rezende, Márcia Kozlowski Junior, Vitoldo Antonio Geus, Juliana Larocca de |
dc.subject.por.fl_str_mv |
Punicaceae Bursera Toxicity Tests. Punicaceae Bursera Pruebas de Toxicidad. Punicaceae Bursera Testes de Toxicidade. |
topic |
Punicaceae Bursera Toxicity Tests. Punicaceae Bursera Pruebas de Toxicidad. Punicaceae Bursera Testes de Toxicidade. |
description |
This work aimed to evaluate the toxicity of the mastic (Myracrodruon urundeuva) and pomegranate (Punica granatum) tinctures through the bioassay with Artemia salina. Different dye concentrations (25, 50, 75, 150, 250, 500, 750 and 1000 μg/mL) were added to the nauplii and the average lethal concentration (CL50) was evaluated using Probit analysis using the StatPlus® software (AnalystSoft, USA), obtaining the upper and lower confidence intervals, after a 24-hour exposure period. The extracts were considered active when the values were < 1000ug/mL. Survival curves and LogRank tests were performed for all extracts at a concentration of 250 μg / mL against negative control (Saline) and positive control (Chlorhexidine), using the GraphPad® Prism software (GraphPad Software, USA). For the mastic, the LC50 was 607.87 ± 38.73, for the pomegranate, 464.98 ± 28.72, both tinctures being considered active. When compared to saline, there was a significant difference in survival curves (p = 0.0206), the same occurred for comparisons with chlorhexidine (p < 0.0001). It can be concluded that mastic and pomegranate tinctures were toxic against Artemia salina in the different concentrations tested. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03-24 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/13751 10.33448/rsd-v10i3.13751 |
url |
https://rsdjournal.org/index.php/rsd/article/view/13751 |
identifier_str_mv |
10.33448/rsd-v10i3.13751 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/13751/14365 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 3; e52010313751 Research, Society and Development; Vol. 10 Núm. 3; e52010313751 Research, Society and Development; v. 10 n. 3; e52010313751 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052747134009344 |