Sexual dimorphism in the assessment of mineral apposition rate during osteoporosis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/12200 |
Resumo: | The objective of this study was to evaluate the daily maxillary and tibial bone mineral apposition rate of ovariectomized rats and orchiectomized rats through confocal laser microscopy. Twenty-four animals were divided into 4 groups (SHAMF, OVX, SHAMM and ORQ). Six rats were distributed to the SHAMF group (submitted to fictitious surgery); six rats to the OVX group (submitted to bilateral ovariectomy); six rats to the SHAMM group (submitted to fictitious surgery) and six rats to the ORQ group (submitted to bilateral orchiectomy). On the 60th day after the surgical procedures the animals received 20 mg/kg of calcein and after 24 days 20 mg/kg of alizarin red was administered. The euthanasia was performed 18 days after the last fluorochrome administration. The histological slides obtained were submitted to confocal microscopy analysis and then dynamic histomorphometry was performed to obtain the daily mineral apposition rate (MAR). In the tibias, the values of MAR were higher for the SHAMF group (P<0.05) (mean: 37.1μm² / day) compared to the ORQ group (mean: 7.16 μm²). In the jaws, the values were higher for the SHAMF group (P<0.05) (mean: 5.175μm² / day) compared to the SHAMM group (mean: 1.84 μm²), OVX (mean: 3.027 μm²) and ORQ group (mean: 1.56 μm²). It can be concluded that the female gender, regarding the characteristics of the maxillary and tibial bones, presented a daily mineral bone apposition rate higher than the male gender, mainly in the maxillary bone, presenting a statistically significant difference between all groups studied. |
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Sexual dimorphism in the assessment of mineral apposition rate during osteoporosisDimorfismo sexual en la evaluación de la tasa de aposición mineral durante la osteoporosisDimorfismo sexual na avaliação da taxa de aposição mineral durante a osteoporose OsteoporoseCaracteres sexuaisMicroscopia.OsteoporosisCaracteres sexualesMicroscopia.OsteoporosisSex characteristicsMicroscopy.The objective of this study was to evaluate the daily maxillary and tibial bone mineral apposition rate of ovariectomized rats and orchiectomized rats through confocal laser microscopy. Twenty-four animals were divided into 4 groups (SHAMF, OVX, SHAMM and ORQ). Six rats were distributed to the SHAMF group (submitted to fictitious surgery); six rats to the OVX group (submitted to bilateral ovariectomy); six rats to the SHAMM group (submitted to fictitious surgery) and six rats to the ORQ group (submitted to bilateral orchiectomy). On the 60th day after the surgical procedures the animals received 20 mg/kg of calcein and after 24 days 20 mg/kg of alizarin red was administered. The euthanasia was performed 18 days after the last fluorochrome administration. The histological slides obtained were submitted to confocal microscopy analysis and then dynamic histomorphometry was performed to obtain the daily mineral apposition rate (MAR). In the tibias, the values of MAR were higher for the SHAMF group (P<0.05) (mean: 37.1μm² / day) compared to the ORQ group (mean: 7.16 μm²). In the jaws, the values were higher for the SHAMF group (P<0.05) (mean: 5.175μm² / day) compared to the SHAMM group (mean: 1.84 μm²), OVX (mean: 3.027 μm²) and ORQ group (mean: 1.56 μm²). It can be concluded that the female gender, regarding the characteristics of the maxillary and tibial bones, presented a daily mineral bone apposition rate higher than the male gender, mainly in the maxillary bone, presenting a statistically significant difference between all groups studied.El objetivo de este estudio fue evaluar la tasa de aposición mineral ósea maxilar y tibial diaria de ratas ovariectomizadas y ratones orquiectomizados usando microscopía láser confocal. Veinticuatro animales fueron divididos en 4 grupos (SHAMF, OVX, SHAMM y ORQ). Se distribuyeron seis ratas al grupo SHAMF (cirugía ficticia); seis ratas al grupo OVX (sometido a una ovariectomía bilateral); seis ratones al grupo SHAMM (cirugía ficticia) y seis ratones al grupo ORQ (sometido a una orquiectomía bilateral). Al 60º día después de los procedimientos quirúrgicos, los animales recibieron 20 mg/kg de calceína y al cabo de 24 días, se administraron 20 mg/kg alizarina. La eutanasia se realizó 18 días después de la última administración de flúor. Los portaobjetos histológicos obtenidos se sometieron a análisis de microscopía confocal y posteriormente se realizó una histomorfometría dinámica para obtener la tasa de aposición mineral diaria (MAR). En la tibia, los valores de MAR fueron más altos para el grupo SHAMF (P<0,05) (media: 37,1μm²/día) en comparación con ORQ (media: 7,16 μm²). En las mandíbulas, los valores fueron más altos para el grupo SHAMF (P<0,05) (media: 5,175μm²/día) en comparación con el grupo SHAMM (media: 1,84 μm²), OVX (media: 3,027 μm²) y el grupo ORQ (media: 1,56 μm²). Se puede concluir que el género femenino, en lo que respecta a las características de los huesos maxilar y tibial, mostró una tasa de aposición mineral ósea diaria superior a la del género masculino, especialmente en el hueso maxilar, mostrando una diferencia estadísticamente entre todos los grupos estudiados.O objetivo desse estudo foi avaliar a taxa de aposição mineral óssea diária maxilar e tibial de ratas ovariectomizadas e ratos orquiectomizados através da microscopia confocal a laser. Vinte e quatro animais foram divididos em 4 grupos (SHAMF, OVX, SHAMM e ORQ). Sendo distribuídos 6 ratas para o grupo SHAMF (submetidas à cirurgia fictícia); 6 ratas para o grupo OVX (submetidas à ovariectomia bilateral); 6 ratos para o grupo SHAMM (submetidos à cirurgia fictícia) e 6 ratos para o grupo ORQ (submetidos à orquiectomia bilateral). No 60º dia após os procedimentos cirúrgicos, os animais receberam 20 mg/kg de calceína e após 24 dias, foi administrado 20 mg/kg de vermelho de alizarina. A eutanásia foi realizada após 18 dias da administração do último fluorocromo. As lâminas histológicas obtidas foram submetidas à análise de microscopia confocal e posteriormente realizado a histomorfometria dinâmica para obtenção da taxa de aposição mineral diária (MAR). Nas tíbias, os valores de MAR foram maiores para o grupo SHAMF (P<0,05) (media: 37,1μm² / dia) em comparação ao grupo ORQ (media: 7,16 μm²). Nas maxilas, os valores foram maiores para o grupo SHAMF (P<0,05) (media: 5,175μm² / dia) em comparação aos grupos SHAMM (media: 1,84 μm²), OVX (media: 3,027 μm²) e ORQ (media: 1,56 μm²). Conclui-se que o gênero feminino, com relação as características do osso maxilar e tibial, apresentou uma taxa de aposição óssea mineral diária superior ao gênero masculino, principalmente no osso maxilar, apresentando diferença estatisticamente significante entre todos os grupos estudados.Research, Society and Development2021-02-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1220010.33448/rsd-v10i2.12200Research, Society and Development; Vol. 10 No. 2; e4910212200Research, Society and Development; Vol. 10 Núm. 2; e4910212200Research, Society and Development; v. 10 n. 2; e49102122002525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/12200/10959Copyright (c) 2021 Juliana Zorzi Coléte; Pedro Henrique Silva Gomes Ferreira; Paula Buzo Frigério; Igor de Oliveira Puttini; Gustavo Momesso; Fábio Roberto de Souza Batista; Roberta Okamotohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessColéte, Juliana ZorziFerreira, Pedro Henrique Silva Gomes Frigério, Paula BuzoPuttini, Igor de Oliveira Momesso, Gustavo Batista, Fábio Roberto de SouzaOkamoto, Roberta 2021-03-02T09:32:39Zoai:ojs.pkp.sfu.ca:article/12200Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:33:50.297221Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Sexual dimorphism in the assessment of mineral apposition rate during osteoporosis Dimorfismo sexual en la evaluación de la tasa de aposición mineral durante la osteoporosis Dimorfismo sexual na avaliação da taxa de aposição mineral durante a osteoporose |
title |
Sexual dimorphism in the assessment of mineral apposition rate during osteoporosis |
spellingShingle |
Sexual dimorphism in the assessment of mineral apposition rate during osteoporosis Coléte, Juliana Zorzi Osteoporose Caracteres sexuais Microscopia. Osteoporosis Caracteres sexuales Microscopia. Osteoporosis Sex characteristics Microscopy. |
title_short |
Sexual dimorphism in the assessment of mineral apposition rate during osteoporosis |
title_full |
Sexual dimorphism in the assessment of mineral apposition rate during osteoporosis |
title_fullStr |
Sexual dimorphism in the assessment of mineral apposition rate during osteoporosis |
title_full_unstemmed |
Sexual dimorphism in the assessment of mineral apposition rate during osteoporosis |
title_sort |
Sexual dimorphism in the assessment of mineral apposition rate during osteoporosis |
author |
Coléte, Juliana Zorzi |
author_facet |
Coléte, Juliana Zorzi Ferreira, Pedro Henrique Silva Gomes Frigério, Paula Buzo Puttini, Igor de Oliveira Momesso, Gustavo Batista, Fábio Roberto de Souza Okamoto, Roberta |
author_role |
author |
author2 |
Ferreira, Pedro Henrique Silva Gomes Frigério, Paula Buzo Puttini, Igor de Oliveira Momesso, Gustavo Batista, Fábio Roberto de Souza Okamoto, Roberta |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Coléte, Juliana Zorzi Ferreira, Pedro Henrique Silva Gomes Frigério, Paula Buzo Puttini, Igor de Oliveira Momesso, Gustavo Batista, Fábio Roberto de Souza Okamoto, Roberta |
dc.subject.por.fl_str_mv |
Osteoporose Caracteres sexuais Microscopia. Osteoporosis Caracteres sexuales Microscopia. Osteoporosis Sex characteristics Microscopy. |
topic |
Osteoporose Caracteres sexuais Microscopia. Osteoporosis Caracteres sexuales Microscopia. Osteoporosis Sex characteristics Microscopy. |
description |
The objective of this study was to evaluate the daily maxillary and tibial bone mineral apposition rate of ovariectomized rats and orchiectomized rats through confocal laser microscopy. Twenty-four animals were divided into 4 groups (SHAMF, OVX, SHAMM and ORQ). Six rats were distributed to the SHAMF group (submitted to fictitious surgery); six rats to the OVX group (submitted to bilateral ovariectomy); six rats to the SHAMM group (submitted to fictitious surgery) and six rats to the ORQ group (submitted to bilateral orchiectomy). On the 60th day after the surgical procedures the animals received 20 mg/kg of calcein and after 24 days 20 mg/kg of alizarin red was administered. The euthanasia was performed 18 days after the last fluorochrome administration. The histological slides obtained were submitted to confocal microscopy analysis and then dynamic histomorphometry was performed to obtain the daily mineral apposition rate (MAR). In the tibias, the values of MAR were higher for the SHAMF group (P<0.05) (mean: 37.1μm² / day) compared to the ORQ group (mean: 7.16 μm²). In the jaws, the values were higher for the SHAMF group (P<0.05) (mean: 5.175μm² / day) compared to the SHAMM group (mean: 1.84 μm²), OVX (mean: 3.027 μm²) and ORQ group (mean: 1.56 μm²). It can be concluded that the female gender, regarding the characteristics of the maxillary and tibial bones, presented a daily mineral bone apposition rate higher than the male gender, mainly in the maxillary bone, presenting a statistically significant difference between all groups studied. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12200 10.33448/rsd-v10i2.12200 |
url |
https://rsdjournal.org/index.php/rsd/article/view/12200 |
identifier_str_mv |
10.33448/rsd-v10i2.12200 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12200/10959 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 2; e4910212200 Research, Society and Development; Vol. 10 Núm. 2; e4910212200 Research, Society and Development; v. 10 n. 2; e4910212200 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052805220925440 |