In vitro culture establishment of Hibiscus sabdariffa L.: obtaining seedlings and induction of callus under different culture conditions aiming at biomass accumulation
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Data de Publicação: | 2021 |
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Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/23114 |
Resumo: | Hibiscus sabdariffa L., is a plant that belong to the family Malvaceae Juss., popularly known as vinagreira, the species has pharmacological importance. The in vitro culture of callus represents an important resource for the continuous production of high quality medicinal compounds, being possible the production of biomass with compounds of medicinal interest. The present work aimed to verify the necessary cultivation conditions for the highest production of wet callogenic mass of explants (hypocotyls and roots). The explants were inoculated in different basic culture media (MS and B5) supplemented with different concentrations of growth regulators and kept in a B.O.D incubator at 30ºC, photoperiod of 16 h of light. Callus production was evaluated by measuring the wet weight of callus after 120 days of cultivation. Based on the results obtained in the study, it can be observed that the proliferation of H. sabdariffa, callus occurred regardless of the type of explants, basal media or concentrations of growth regulators, 2,4-D and BAP, but the best results for the production of callogenic masses of hypocotyls and roots were in MS medium supplemented with 0,1 mg.L-1 of 2,4-D in combination with 0,1 mg.L-1 of BAP (T2), presenting averages of callogenic masses from hypocotyl explants (7,43 ± 0.19 g) and roots (6,75 ± 0.07 g). The results show that in vitro techniques have advantages of biomass production in sterile and controlled environmental conditions, allowing a better control of the development processes and production of tissues rich in bioactive compounds. |
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In vitro culture establishment of Hibiscus sabdariffa L.: obtaining seedlings and induction of callus under different culture conditions aiming at biomass accumulationEstablecimiento de cultura in vitro de Hibiscus sabdariffa L.: obtención de plántulas e inducción de callos en diferentes condiciones de cultivo con el objetivo de la acumulación de biomasaEstabelecimento de cultura in vitro de Hibiscus sabdariffa L.: obtenção de plântulas e indução de calos em diferentes condições de cultura visando o acúmulo de biomassaBiotecnologíaCallogénesisMedios de culturaReguladores de crecimiento.CallogenesisCulture mediaBiotecnologyGrowth regulators. BietecnologiaCalogêneseMeios de culturaReguladores de crescimento. Hibiscus sabdariffa L., is a plant that belong to the family Malvaceae Juss., popularly known as vinagreira, the species has pharmacological importance. The in vitro culture of callus represents an important resource for the continuous production of high quality medicinal compounds, being possible the production of biomass with compounds of medicinal interest. The present work aimed to verify the necessary cultivation conditions for the highest production of wet callogenic mass of explants (hypocotyls and roots). The explants were inoculated in different basic culture media (MS and B5) supplemented with different concentrations of growth regulators and kept in a B.O.D incubator at 30ºC, photoperiod of 16 h of light. Callus production was evaluated by measuring the wet weight of callus after 120 days of cultivation. Based on the results obtained in the study, it can be observed that the proliferation of H. sabdariffa, callus occurred regardless of the type of explants, basal media or concentrations of growth regulators, 2,4-D and BAP, but the best results for the production of callogenic masses of hypocotyls and roots were in MS medium supplemented with 0,1 mg.L-1 of 2,4-D in combination with 0,1 mg.L-1 of BAP (T2), presenting averages of callogenic masses from hypocotyl explants (7,43 ± 0.19 g) and roots (6,75 ± 0.07 g). The results show that in vitro techniques have advantages of biomass production in sterile and controlled environmental conditions, allowing a better control of the development processes and production of tissues rich in bioactive compounds.Hibiscus sabdariffa L., és una planta que perteneciente a la familia Malvaceae Juss., popularmente conocida como vinagreira, la especie tiene importancia farmacológica. El cultivo in vitro de callos representa un recurso importante para la producción continua de compuestos medicinales de alta calidad, siendo posible la producción de biomasa con compuestos de interés medicinal. El presente trabajo tenía como objetivo verificar las condiciones de cultivo necesarias para la mayor producción de masa callogénica húmeda de explantes (hipocotilos y raíces). Los explantes fueron inoculados en diferentes medios de cultivo básico (MS y B5) complementados con diferentes concentraciones de reguladores de crecimiento y mantenidos en una incubadora B.O.D a 30ºC, fotoperiodo de 16 h de luz. La producción de callos se evaluó midiendo el peso húmedo de los callos después de 120 días de cultivo. Sobre la base de los resultados obtenidos en el estudio, se puede observar que la proliferación de callos de H. sabdariffa, ocurrió independientemente de los tipos de explantes, medios basales o concentraciones de reguladores de crecimiento, 2,4-D y BAP, pero los mejores resultados para la producción de masas callogénicas de hipocotilos y raíces fueron en MS medio complementado con 0,1 mg.L-1 de 2,4-D en combinación con 0,1 mg.L-1 de BAP (T2), presentando promedios de masas callogénicas de explantes de hipocotilo (7,43 ± 0,19 g) y raíces (6,75 ± 0,07 g). Los resultados muestran que las técnicas in vitro tienen ventajas de la producción de biomasa en condiciones ambientales estériles y controladas, permitiendo un mejor control de los procesos de desarrollo y producción de tejidos ricos en compuestos bioactivos.Hibiscus sabdariffa L., é uma planta pertencente à família Malvaceae Juss., conhecida popularmente como vinagreira, a espécie apresenta importância farmacológica. A cultura in vitro de calos representa um importante recurso para a produção contínua de compostos medicinais de alta qualidade, sendo possível a produção de biomassa com estes. O presente trabalho teve como objetivo verificar quais as condições necessárias de cultivo para a maior produção de massa calogênica úmida dos explantes (hipocótilos e raízes). Os explantes foram inoculados em diferentes meios básicos de cultura (MS e B5) suplementados com diferentes concentrações de reguladores de crescimento e mantidos em incubadora tipo B.O.D a 30ºC, fotoperíodo de 16 h de luz. A produção de calos foi avaliada através de aferição do peso úmido dos calos, após 120 dias de cultivo. Com base nos resultados obtidos no estudo, pode-se observar que a proliferação de calos de H. sabdariffa, ocorreu independente dos tipos de explantes, meios basais ou concentrações de reguladores de crescimento, 2,4-D e BAP, porém os melhores resultados para as produções de massas calogênicas dos hipocótilos e raízes foram em meio MS suplementado com 0,1 mg.L-1 de 2,4-D em combinação com 0,1 mg.L-1de BAP (T2), apresentando médias de massas calogênicas provenientes de explantes de hipocótilos (7,43 ± 0,19 g) e de raízes (6,75 ± 0,07 g). Os resultados mostram que as técnicas in vitro, apresentam vantagens de produção de biomassa em condições ambientais estéreis e controladas, permitindo um melhor controle dos processos de desenvolvimento e produção de tecidos ricos em compostos bioativos.Research, Society and Development2021-11-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2311410.33448/rsd-v10i15.23114Research, Society and Development; Vol. 10 No. 15; e358101523114Research, Society and Development; Vol. 10 Núm. 15; e358101523114Research, Society and Development; v. 10 n. 15; e3581015231142525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/23114/20404Copyright (c) 2021 Alessandra Carla Guimarães Sobrinho; Alberdan Silva Santos https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSobrinho, Alessandra Carla GuimarãesSilva Santos , Alberdan 2021-12-06T10:13:53Zoai:ojs.pkp.sfu.ca:article/23114Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:42:05.105224Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
In vitro culture establishment of Hibiscus sabdariffa L.: obtaining seedlings and induction of callus under different culture conditions aiming at biomass accumulation Establecimiento de cultura in vitro de Hibiscus sabdariffa L.: obtención de plántulas e inducción de callos en diferentes condiciones de cultivo con el objetivo de la acumulación de biomasa Estabelecimento de cultura in vitro de Hibiscus sabdariffa L.: obtenção de plântulas e indução de calos em diferentes condições de cultura visando o acúmulo de biomassa |
title |
In vitro culture establishment of Hibiscus sabdariffa L.: obtaining seedlings and induction of callus under different culture conditions aiming at biomass accumulation |
spellingShingle |
In vitro culture establishment of Hibiscus sabdariffa L.: obtaining seedlings and induction of callus under different culture conditions aiming at biomass accumulation Sobrinho, Alessandra Carla Guimarães Biotecnología Callogénesis Medios de cultura Reguladores de crecimiento. Callogenesis Culture media Biotecnology Growth regulators. Bietecnologia Calogênese Meios de cultura Reguladores de crescimento. |
title_short |
In vitro culture establishment of Hibiscus sabdariffa L.: obtaining seedlings and induction of callus under different culture conditions aiming at biomass accumulation |
title_full |
In vitro culture establishment of Hibiscus sabdariffa L.: obtaining seedlings and induction of callus under different culture conditions aiming at biomass accumulation |
title_fullStr |
In vitro culture establishment of Hibiscus sabdariffa L.: obtaining seedlings and induction of callus under different culture conditions aiming at biomass accumulation |
title_full_unstemmed |
In vitro culture establishment of Hibiscus sabdariffa L.: obtaining seedlings and induction of callus under different culture conditions aiming at biomass accumulation |
title_sort |
In vitro culture establishment of Hibiscus sabdariffa L.: obtaining seedlings and induction of callus under different culture conditions aiming at biomass accumulation |
author |
Sobrinho, Alessandra Carla Guimarães |
author_facet |
Sobrinho, Alessandra Carla Guimarães Silva Santos , Alberdan |
author_role |
author |
author2 |
Silva Santos , Alberdan |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Sobrinho, Alessandra Carla Guimarães Silva Santos , Alberdan |
dc.subject.por.fl_str_mv |
Biotecnología Callogénesis Medios de cultura Reguladores de crecimiento. Callogenesis Culture media Biotecnology Growth regulators. Bietecnologia Calogênese Meios de cultura Reguladores de crescimento. |
topic |
Biotecnología Callogénesis Medios de cultura Reguladores de crecimiento. Callogenesis Culture media Biotecnology Growth regulators. Bietecnologia Calogênese Meios de cultura Reguladores de crescimento. |
description |
Hibiscus sabdariffa L., is a plant that belong to the family Malvaceae Juss., popularly known as vinagreira, the species has pharmacological importance. The in vitro culture of callus represents an important resource for the continuous production of high quality medicinal compounds, being possible the production of biomass with compounds of medicinal interest. The present work aimed to verify the necessary cultivation conditions for the highest production of wet callogenic mass of explants (hypocotyls and roots). The explants were inoculated in different basic culture media (MS and B5) supplemented with different concentrations of growth regulators and kept in a B.O.D incubator at 30ºC, photoperiod of 16 h of light. Callus production was evaluated by measuring the wet weight of callus after 120 days of cultivation. Based on the results obtained in the study, it can be observed that the proliferation of H. sabdariffa, callus occurred regardless of the type of explants, basal media or concentrations of growth regulators, 2,4-D and BAP, but the best results for the production of callogenic masses of hypocotyls and roots were in MS medium supplemented with 0,1 mg.L-1 of 2,4-D in combination with 0,1 mg.L-1 of BAP (T2), presenting averages of callogenic masses from hypocotyl explants (7,43 ± 0.19 g) and roots (6,75 ± 0.07 g). The results show that in vitro techniques have advantages of biomass production in sterile and controlled environmental conditions, allowing a better control of the development processes and production of tissues rich in bioactive compounds. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/23114 10.33448/rsd-v10i15.23114 |
url |
https://rsdjournal.org/index.php/rsd/article/view/23114 |
identifier_str_mv |
10.33448/rsd-v10i15.23114 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/23114/20404 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Alessandra Carla Guimarães Sobrinho; Alberdan Silva Santos https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Alessandra Carla Guimarães Sobrinho; Alberdan Silva Santos https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 15; e358101523114 Research, Society and Development; Vol. 10 Núm. 15; e358101523114 Research, Society and Development; v. 10 n. 15; e358101523114 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052696679677952 |