Production of Lactobacillus rhamnosus BRM 029693 in feed-batch fermentation

Detalhes bibliográficos
Autor(a) principal: Freitas, Rafaella Martins de
Data de Publicação: 2020
Outros Autores: Carvalho, Juliane Döering Gasparin, Bruno, Laura Maria, Pinto, Gustavo Adolfo Saavedra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/4280
Resumo: The objective of this work was to produce Lactobacillus rhamnosus BRM 029693 biomass in a fermenter with the aim to expand its production scale. Lactobacillus rhamnosus BRM 029693 was grown in a fermenter under different aeration conditions (in surface and depth) using a commercial substrate (MRS broth) and simplified substrate formulated in the laboratory itself, and under different pH conditions (4.7, 5.2, 5.7). The highest biomass production on a fermenter scale occurred using synthetic MRS, 1 v.v.m. aeration on the medium surface and without pH control (3.2 g/L) among all the tests. The biomass production was only 0.7 g/L when the MRS medium was replaced by simplified MRS. The pH also influenced the biomass production, reaching 0.9 g/L when the fermentation pH was controlled at pH 4.7.
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spelling Production of Lactobacillus rhamnosus BRM 029693 in feed-batch fermentationProducción de Lactobacillus rhamnosus BRM 029693 en fermentación por lote de alimentaciónProdução de Lactobacillus rhamnosus BRM 029693 em fermentação em bateladaBiomasaCultivo de LactobacillusBactéria del ácido láctico.BiomassLactobacillus growthLactic acid bacteria.BiomassaCultivo de LactobacillusBactéria ácido lática.The objective of this work was to produce Lactobacillus rhamnosus BRM 029693 biomass in a fermenter with the aim to expand its production scale. Lactobacillus rhamnosus BRM 029693 was grown in a fermenter under different aeration conditions (in surface and depth) using a commercial substrate (MRS broth) and simplified substrate formulated in the laboratory itself, and under different pH conditions (4.7, 5.2, 5.7). The highest biomass production on a fermenter scale occurred using synthetic MRS, 1 v.v.m. aeration on the medium surface and without pH control (3.2 g/L) among all the tests. The biomass production was only 0.7 g/L when the MRS medium was replaced by simplified MRS. The pH also influenced the biomass production, reaching 0.9 g/L when the fermentation pH was controlled at pH 4.7.O objetivo deste trabalho foi produzir biomassa de Lactobacillus, rhamnosus BRM 029693, em fermentador, con el intuito de ampliar su escala de producción. Lactobacillus rhamnosus BRM 029693, foi cultivado em fermentador em diferentes condições de aeração (em superfície e profundidade), empregado um substrato comercial (caldo MRS) y substrato simplificado formulado no próprio laboratório; e em diferentes condiciones de pH (4,7, 5,2, 5,7). Una producción de biomasa en escala de fermentador utilizando MRS sintético, emisión de 1 v.v.m. na superfície do meio and sem control of pH foi a maior (3,2 g / L) entre todos los ensaios. Quando o meio MRS foi sustituído por MRS simplificado, una producción de biomasa de apenas 0,7 g / L. El pH también puede influir en la producción de biomasa, que es de 0,9 g / L, o el pH de la fermentación controlada de pH 4,7.O objetivo deste trabalho foi produzir biomassa de Lactobacillus, rhamnosus BRM 029693, em fermentador, com o intuito de ampliar sua escala de produção. Lactobacillus rhamnosus BRM 029693, foi cultivado em fermentador em diferentes condições de aeração (em superfície e profundidade), empregado um substrato comercial (caldo MRS) e substrato simplificado formulado no próprio laboratório; e em diferentes condições de pH (4,7, 5,2, 5,7). A produção de biomassa em escala de fermentador utilizando o MRS sintético, aeração de 1 v.v.m. na superfície do meio e sem controle de pH foi a maior (3,2 g/L) entre todos os ensaios. Quando o meio MRS foi substituído por MRS simplificado, a produção de biomassa foi de apenas 0,7 g/L. O pH também influenciou na produção de biomassa, que foi de 0,9 g/L quando o pH da fermentação foi controlado em pH 4,7. Research, Society and Development2020-05-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/428010.33448/rsd-v9i7.4280Research, Society and Development; Vol. 9 No. 7; e531974280Research, Society and Development; Vol. 9 Núm. 7; e531974280Research, Society and Development; v. 9 n. 7; e5319742802525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/4280/4266Copyright (c) 2020 Rafaella Martins de Freitas, Juliane Döering Gasparin Carvalho, Laura Maria Bruno, Gustavo Adolfo Saavedra Pintoinfo:eu-repo/semantics/openAccessFreitas, Rafaella Martins deCarvalho, Juliane Döering GasparinBruno, Laura MariaPinto, Gustavo Adolfo Saavedra2020-08-20T18:05:03Zoai:ojs.pkp.sfu.ca:article/4280Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:28:11.264072Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Production of Lactobacillus rhamnosus BRM 029693 in feed-batch fermentation
Producción de Lactobacillus rhamnosus BRM 029693 en fermentación por lote de alimentación
Produção de Lactobacillus rhamnosus BRM 029693 em fermentação em batelada
title Production of Lactobacillus rhamnosus BRM 029693 in feed-batch fermentation
spellingShingle Production of Lactobacillus rhamnosus BRM 029693 in feed-batch fermentation
Freitas, Rafaella Martins de
Biomasa
Cultivo de Lactobacillus
Bactéria del ácido láctico.
Biomass
Lactobacillus growth
Lactic acid bacteria.
Biomassa
Cultivo de Lactobacillus
Bactéria ácido lática.
title_short Production of Lactobacillus rhamnosus BRM 029693 in feed-batch fermentation
title_full Production of Lactobacillus rhamnosus BRM 029693 in feed-batch fermentation
title_fullStr Production of Lactobacillus rhamnosus BRM 029693 in feed-batch fermentation
title_full_unstemmed Production of Lactobacillus rhamnosus BRM 029693 in feed-batch fermentation
title_sort Production of Lactobacillus rhamnosus BRM 029693 in feed-batch fermentation
author Freitas, Rafaella Martins de
author_facet Freitas, Rafaella Martins de
Carvalho, Juliane Döering Gasparin
Bruno, Laura Maria
Pinto, Gustavo Adolfo Saavedra
author_role author
author2 Carvalho, Juliane Döering Gasparin
Bruno, Laura Maria
Pinto, Gustavo Adolfo Saavedra
author2_role author
author
author
dc.contributor.author.fl_str_mv Freitas, Rafaella Martins de
Carvalho, Juliane Döering Gasparin
Bruno, Laura Maria
Pinto, Gustavo Adolfo Saavedra
dc.subject.por.fl_str_mv Biomasa
Cultivo de Lactobacillus
Bactéria del ácido láctico.
Biomass
Lactobacillus growth
Lactic acid bacteria.
Biomassa
Cultivo de Lactobacillus
Bactéria ácido lática.
topic Biomasa
Cultivo de Lactobacillus
Bactéria del ácido láctico.
Biomass
Lactobacillus growth
Lactic acid bacteria.
Biomassa
Cultivo de Lactobacillus
Bactéria ácido lática.
description The objective of this work was to produce Lactobacillus rhamnosus BRM 029693 biomass in a fermenter with the aim to expand its production scale. Lactobacillus rhamnosus BRM 029693 was grown in a fermenter under different aeration conditions (in surface and depth) using a commercial substrate (MRS broth) and simplified substrate formulated in the laboratory itself, and under different pH conditions (4.7, 5.2, 5.7). The highest biomass production on a fermenter scale occurred using synthetic MRS, 1 v.v.m. aeration on the medium surface and without pH control (3.2 g/L) among all the tests. The biomass production was only 0.7 g/L when the MRS medium was replaced by simplified MRS. The pH also influenced the biomass production, reaching 0.9 g/L when the fermentation pH was controlled at pH 4.7.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-25
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/4280
10.33448/rsd-v9i7.4280
url https://rsdjournal.org/index.php/rsd/article/view/4280
identifier_str_mv 10.33448/rsd-v9i7.4280
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/4280/4266
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 7; e531974280
Research, Society and Development; Vol. 9 Núm. 7; e531974280
Research, Society and Development; v. 9 n. 7; e531974280
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052736142835712

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