Perfil proteômico do corpo gorduroso de larvas de Diatraea saccharalis (Lepidoptera: Crambidae) sob condição de injúria séptica causada por microorganismos
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UNIOESTE |
Texto Completo: | http://tede.unioeste.br/handle/tede/3701 |
Resumo: | Diatraea saccharalis, known as a sugarcane borer, is a moth of the Lepidoptera order, being one of the main pests on sugarcane, responsible for damage to the sugar and alcohol industries. The fat body is the organ responsible for several functions during the larval phase of insects, mainly in charge of synthesizing molecules with metabolic actions. The success in insects’ adaptation is due to its efficient defense system, in which the fat body is able to synthesize antimicrobial peptides (AMP’s) and secrete them in the hemolymph, where they act as a defense mechanism against pathogens. AMP’s has a cationic character and present the capacity to interact with negative charge molecules of the bacteria, leading to the disintegration of their membranes. Considering the concerns regarding bacterial resistance to antibiotics and antimicrobial properties of AMP’s, such as fast-acting, varied mechanisms of action and wide range against Gram-positive and Gram-negative bacteria; it has become appropriate to carry out studies to use AMP’s as alternative or complementary antibiotic. In D. saccharalis, AMP’s with antimicrobial action have been reported in recently published papers using proteomic methodology. Thereby, this study aimed to perform the differential analysis of the proteins and peptides of the fat body in the 5th instar of larvae challenged with Escherichia coli, Bacillus subtilis and Beauveria bassiana; using the two-dimensional electrophoresis technique (2-DE), with 12.5% SDS-PAGE tricine gels, MALDI-TOF type mass spectrometry and the research in protein databases (Mascot and TagIdent). After analysis, six defense proteins of the innate immune system were found using the TagIdent identification tool: apolipophorin-3, attacin, attacin-F, Peptidoglycan recognition protein (PGRPs), putative defense protein 1 and putative defense protein 3. The AMP attacin presented a positive regulation in relation to the control gel indicating that the B. bassiana septic challenge stimulated the immune system of D. saccharalis. This study provides the first reports of proteins of the immune system produced by the adipose tissue of D. saccharalis. |
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Silva , José Luis da Conceiçãohttp://lattes.cnpq.br/9251091711372741Silva , José Luis da Conceiçãohttp://lattes.cnpq.br/9251091711372741Kadowaki , Marina Kimikohttp://lattes.cnpq.br/1819723253019762Rosado, Fabio Rogeriohttp://lattes.cnpq.br/2173353919611485http://lattes.cnpq.br/5044075208062609Seuchuco, Charles2018-05-24T20:32:41Z2018-03-05SEUCHUCO, Charles. Perfil proteômico do corpo gorduroso de larvas de Diatraea saccharalis (Lepidoptera: Crambidae) sob condição de injúria séptica causada por microorganismos. 2018. 52 f. Dissertação ( Mestrado em Ciências Farmacêuticas) - Universidade Estadual do Oeste do Paraná, Cascavel, 2018.http://tede.unioeste.br/handle/tede/3701Diatraea saccharalis, known as a sugarcane borer, is a moth of the Lepidoptera order, being one of the main pests on sugarcane, responsible for damage to the sugar and alcohol industries. The fat body is the organ responsible for several functions during the larval phase of insects, mainly in charge of synthesizing molecules with metabolic actions. The success in insects’ adaptation is due to its efficient defense system, in which the fat body is able to synthesize antimicrobial peptides (AMP’s) and secrete them in the hemolymph, where they act as a defense mechanism against pathogens. AMP’s has a cationic character and present the capacity to interact with negative charge molecules of the bacteria, leading to the disintegration of their membranes. Considering the concerns regarding bacterial resistance to antibiotics and antimicrobial properties of AMP’s, such as fast-acting, varied mechanisms of action and wide range against Gram-positive and Gram-negative bacteria; it has become appropriate to carry out studies to use AMP’s as alternative or complementary antibiotic. In D. saccharalis, AMP’s with antimicrobial action have been reported in recently published papers using proteomic methodology. Thereby, this study aimed to perform the differential analysis of the proteins and peptides of the fat body in the 5th instar of larvae challenged with Escherichia coli, Bacillus subtilis and Beauveria bassiana; using the two-dimensional electrophoresis technique (2-DE), with 12.5% SDS-PAGE tricine gels, MALDI-TOF type mass spectrometry and the research in protein databases (Mascot and TagIdent). After analysis, six defense proteins of the innate immune system were found using the TagIdent identification tool: apolipophorin-3, attacin, attacin-F, Peptidoglycan recognition protein (PGRPs), putative defense protein 1 and putative defense protein 3. The AMP attacin presented a positive regulation in relation to the control gel indicating that the B. bassiana septic challenge stimulated the immune system of D. saccharalis. This study provides the first reports of proteins of the immune system produced by the adipose tissue of D. saccharalis.A Diatraea saccharalis, conhecida como broca-da-cana, é uma mariposa da ordem Lepidoptera, tratando-se de uma das principais pragas da cana-de-açúcar, responsável por prejuízos à indústria de açúcar e de álcool. O corpo gorduroso é o órgão responsável por várias funções durante a fase larval dos insetos, principalmente encarregado de sintetizar moléculas com ações metabólicas. O sucesso na adaptação dos insetos se deve ao seu eficiente sistema de defesa, no qual o tecido gorduroso é capaz de sintetizar peptídeos antimicrobianos (PAM’s) e secretá-los na hemolinfa, onde atuam como mecanismo de defesa contra patógenos. Os PAM’s têm caráter catiônico e apresentam capacidade de interagir com as moléculas de carga negativas das bactérias, levando a desintegração de suas membranas. Considerando as preocupações em relação à resistência bacteriana a antibióticos e às propriedades antimicrobianas dos PAM’s como ação rápida, mecanismos de ação variados e amplo espectro contra bactérias Gram-positivas e Gram-negativas, têm se tornado atrativo para a realização de estudos para utilização de PAM’s como antibiótico alternativo ou complementar. Em D. saccharalis já foram relatados PAM’s com ação antimicrobiana em artigos recentemente publicados empregando metodologia proteômica. Desta forma, este trabalho teve como objetivo realizar a análise diferencial das proteínas e peptídeos do corpo gorduroso no 5° instar de larvas controles e desafiadas com Escherichia coli, Bacillus subtilis e Beauveria bassiana, utilizando eletroforese bidimensional (2-DE) com géis tricina SDS-PAGE 12,5%, espectrometria de massas do tipo MALDI-ToF e a pesquisa em bancos de dados de proteínas (Mascot e TagIdent). Após análise, foram encontradas seis proteínas de defesa do sistema imune inato utilizando a ferramenta de identificação TagIdent, sendo elas Apolipophorin-3, Atacina, Atacina-F, Proteína de reconhecimento de peptidoglicano (PGRPs), Provável proteína de defesa 1 e Provável proteína de defesa 3. O PAM Atacina apresentou regulação positiva em relação ao gel controle, indicando que o desafio séptico por B. bassiana estimulou o sistema imune da D. saccharalis. Este trabalho fornece os primeiros relatos de proteínas do sistema imune produzidas pelo corpo gorduroso de D. saccharalis.Submitted by Rosangela Silva (rosangela.silva3@unioeste.br) on 2018-05-24T20:32:41Z No. of bitstreams: 2 Charles Seuchuco.pdf: 1461597 bytes, checksum: d1fe07c8c4b16541956c362cc1acb19c (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-05-24T20:32:41Z (GMT). No. of bitstreams: 2 Charles Seuchuco.pdf: 1461597 bytes, checksum: d1fe07c8c4b16541956c362cc1acb19c (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-03-05application/pdfpor6588633818200016417500Universidade Estadual do Oeste do ParanáCascavelPrograma de Pós-Graduação em Ciências FarmacêuticasUNIOESTEBrasilCentro de Ciências Médicas e Farmacêuticashttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessPeptídeos antimicrobianosDiatraea saccharalisMALDI-ToFEletroforeseAntimicrobial peptidesDiatraea saccharalisElectrophoresisCIENCIAS DA SAUDE::FARMACIAPerfil proteômico do corpo gorduroso de larvas de Diatraea saccharalis (Lepidoptera: Crambidae) sob condição de injúria séptica causada por microorganismosProteomic profile of adipose tissue of Diatraea saccharalis larvae (Lepidoptera: crambidae) under septic injury caused by microorganismsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis7878055067573953101600600600-89404397133878492676997636413449754996reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALCharles Seuchuco.pdfCharles Seuchuco.pdfapplication/pdf1461597http://tede.unioeste.br:8080/tede/bitstream/tede/3701/5/Charles+Seuchuco.pdfd1fe07c8c4b16541956c362cc1acb19cMD55CC-LICENSElicense_urllicense_urltext/plain; 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dc.title.por.fl_str_mv |
Perfil proteômico do corpo gorduroso de larvas de Diatraea saccharalis (Lepidoptera: Crambidae) sob condição de injúria séptica causada por microorganismos |
dc.title.alternative.eng.fl_str_mv |
Proteomic profile of adipose tissue of Diatraea saccharalis larvae (Lepidoptera: crambidae) under septic injury caused by microorganisms |
title |
Perfil proteômico do corpo gorduroso de larvas de Diatraea saccharalis (Lepidoptera: Crambidae) sob condição de injúria séptica causada por microorganismos |
spellingShingle |
Perfil proteômico do corpo gorduroso de larvas de Diatraea saccharalis (Lepidoptera: Crambidae) sob condição de injúria séptica causada por microorganismos Seuchuco, Charles Peptídeos antimicrobianos Diatraea saccharalis MALDI-ToF Eletroforese Antimicrobial peptides Diatraea saccharalis Electrophoresis CIENCIAS DA SAUDE::FARMACIA |
title_short |
Perfil proteômico do corpo gorduroso de larvas de Diatraea saccharalis (Lepidoptera: Crambidae) sob condição de injúria séptica causada por microorganismos |
title_full |
Perfil proteômico do corpo gorduroso de larvas de Diatraea saccharalis (Lepidoptera: Crambidae) sob condição de injúria séptica causada por microorganismos |
title_fullStr |
Perfil proteômico do corpo gorduroso de larvas de Diatraea saccharalis (Lepidoptera: Crambidae) sob condição de injúria séptica causada por microorganismos |
title_full_unstemmed |
Perfil proteômico do corpo gorduroso de larvas de Diatraea saccharalis (Lepidoptera: Crambidae) sob condição de injúria séptica causada por microorganismos |
title_sort |
Perfil proteômico do corpo gorduroso de larvas de Diatraea saccharalis (Lepidoptera: Crambidae) sob condição de injúria séptica causada por microorganismos |
author |
Seuchuco, Charles |
author_facet |
Seuchuco, Charles |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva , José Luis da Conceição |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9251091711372741 |
dc.contributor.referee1.fl_str_mv |
Silva , José Luis da Conceição |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/9251091711372741 |
dc.contributor.referee2.fl_str_mv |
Kadowaki , Marina Kimiko |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1819723253019762 |
dc.contributor.referee3.fl_str_mv |
Rosado, Fabio Rogerio |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/2173353919611485 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5044075208062609 |
dc.contributor.author.fl_str_mv |
Seuchuco, Charles |
contributor_str_mv |
Silva , José Luis da Conceição Silva , José Luis da Conceição Kadowaki , Marina Kimiko Rosado, Fabio Rogerio |
dc.subject.por.fl_str_mv |
Peptídeos antimicrobianos Diatraea saccharalis MALDI-ToF Eletroforese |
topic |
Peptídeos antimicrobianos Diatraea saccharalis MALDI-ToF Eletroforese Antimicrobial peptides Diatraea saccharalis Electrophoresis CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Antimicrobial peptides Diatraea saccharalis Electrophoresis |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::FARMACIA |
description |
Diatraea saccharalis, known as a sugarcane borer, is a moth of the Lepidoptera order, being one of the main pests on sugarcane, responsible for damage to the sugar and alcohol industries. The fat body is the organ responsible for several functions during the larval phase of insects, mainly in charge of synthesizing molecules with metabolic actions. The success in insects’ adaptation is due to its efficient defense system, in which the fat body is able to synthesize antimicrobial peptides (AMP’s) and secrete them in the hemolymph, where they act as a defense mechanism against pathogens. AMP’s has a cationic character and present the capacity to interact with negative charge molecules of the bacteria, leading to the disintegration of their membranes. Considering the concerns regarding bacterial resistance to antibiotics and antimicrobial properties of AMP’s, such as fast-acting, varied mechanisms of action and wide range against Gram-positive and Gram-negative bacteria; it has become appropriate to carry out studies to use AMP’s as alternative or complementary antibiotic. In D. saccharalis, AMP’s with antimicrobial action have been reported in recently published papers using proteomic methodology. Thereby, this study aimed to perform the differential analysis of the proteins and peptides of the fat body in the 5th instar of larvae challenged with Escherichia coli, Bacillus subtilis and Beauveria bassiana; using the two-dimensional electrophoresis technique (2-DE), with 12.5% SDS-PAGE tricine gels, MALDI-TOF type mass spectrometry and the research in protein databases (Mascot and TagIdent). After analysis, six defense proteins of the innate immune system were found using the TagIdent identification tool: apolipophorin-3, attacin, attacin-F, Peptidoglycan recognition protein (PGRPs), putative defense protein 1 and putative defense protein 3. The AMP attacin presented a positive regulation in relation to the control gel indicating that the B. bassiana septic challenge stimulated the immune system of D. saccharalis. This study provides the first reports of proteins of the immune system produced by the adipose tissue of D. saccharalis. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-05-24T20:32:41Z |
dc.date.issued.fl_str_mv |
2018-03-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SEUCHUCO, Charles. Perfil proteômico do corpo gorduroso de larvas de Diatraea saccharalis (Lepidoptera: Crambidae) sob condição de injúria séptica causada por microorganismos. 2018. 52 f. Dissertação ( Mestrado em Ciências Farmacêuticas) - Universidade Estadual do Oeste do Paraná, Cascavel, 2018. |
dc.identifier.uri.fl_str_mv |
http://tede.unioeste.br/handle/tede/3701 |
identifier_str_mv |
SEUCHUCO, Charles. Perfil proteômico do corpo gorduroso de larvas de Diatraea saccharalis (Lepidoptera: Crambidae) sob condição de injúria séptica causada por microorganismos. 2018. 52 f. Dissertação ( Mestrado em Ciências Farmacêuticas) - Universidade Estadual do Oeste do Paraná, Cascavel, 2018. |
url |
http://tede.unioeste.br/handle/tede/3701 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
7878055067573953101 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
-8940439713387849267 |
dc.relation.cnpq.fl_str_mv |
6997636413449754996 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual do Oeste do Paraná Cascavel |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Farmacêuticas |
dc.publisher.initials.fl_str_mv |
UNIOESTE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Centro de Ciências Médicas e Farmacêuticas |
publisher.none.fl_str_mv |
Universidade Estadual do Oeste do Paraná Cascavel |
dc.source.none.fl_str_mv |
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