Métodos de inoculação de Azospirillum brasilense e sua influência na promoção de crescimento do milho

Detalhes bibliográficos
Autor(a) principal: Brito, Tauane Santos
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UNIOESTE
Texto Completo: http://tede.unioeste.br/handle/tede/4278
Resumo: The use of plant growth promoting bacteria in maize stands out as an alternative to increase productivity, reduce the use of chemicals and preserve the environment. However, more efficient methods for the use of inoculant are sought, especially in the stage of management by the producer, seeking to preserve the microorganism’s viability. The objective of this study was to evaluate the influence of inoculation methods with Azospirillum brasilense in the metabolism and the plant growth promotion in maize hybrid Formula VT®. In greenhouse, using as experimental unit polyethylene pots with 25 liter capacity, filled with Oxisol, were cultivated maize plants in function of different inoculation methods with the strains Abv5 and Abv% from the plant growth promoting bacteria A. brasilense. The inoculation methods consisted in the absence of inoculation (control), seed inoculation (during the seeding moment), foliar inoculation (in the phenological stage V4) and seed inoculation associated to foliar inoculation (during the seeding moment and in the phenological stage V4, respectively). The plant’s chlorophyll relative content was fortnightly evaluated. In the phenological stages V8, VT and R6 was measured the readings of the CO2 liquid assimilation rate in function of different flux densities of photosynthetically active photons. From these data it was determined the CO2 compensation point, the quantum yield and the maximum CO2 liquid assimilation. Still in the phenological stages V8, VT and R6, it was measured the number of leaves, aerial height, basal stem diameter, root volume, dry mass of leaves, stem+leaf sheath, roots and total dry mass, foliar area and foliar contents of nitrogen, phosphorus and potassium. Finally, in the phenological stage R6 it was evaluated the ear diameter, ear length, number of rows, number of kernels per row, dry mass of the reproductive structure, 100 grain mass, total grain mass and the grain content of nitrogen, phosphorus and potassium. Plants with inoculation via seed and seed inoculation associated to foliar inoculation stood out as to basal stem diameter, leaves dry mass, root volume and the contents of nitrogen, phosphorus and potassium. Still, the inoculated plants showed a differentiated capacity of transport of electrons and efficiency of CO2 liquid assimilation rate in relation to the control in all evaluated stages. The inoculation method via foliar influenced in the adaptative demand of the plants, reducing their development speed in the initial stage. The inoculation methods influenced in physiological, morphometric and nutritional parameters of the development of maize, however, they did not had any influence in the final yield. The plant development related to the growth promotion was positively influenced by the inoculation, varying according to the utilized method.
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spelling Guimarães, Vandeir Franciscohttp://lattes.cnpq.br/8187147768335662Braga, Gilberto Costahttp://lattes.cnpq.br/5595085713178176Chaves, Elisiane Inês Dall’ogliohttp://lattes.cnpq.br/4193352042397738Guimarães, Vandeir Franciscohttp://lattes.cnpq.br/8187147768335662Ruppenthal, Vivianehttp://lattes.cnpq.br/6613787394484578Nozaki, Márcia de Holandahttp://lattes.cnpq.br/4976846609735019http://lattes.cnpq.br/9985675185784615Brito, Tauane Santos2019-05-17T22:29:26Z2019-02-25BRITO, Tauane Santos. Métodos de inoculação de Azospirillum brasilense e sua influência na promoção de crescimento do milho. 2019. 75 f. Dissertação (Mestrado em Agronomia) - Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon, 2019.http://tede.unioeste.br/handle/tede/4278The use of plant growth promoting bacteria in maize stands out as an alternative to increase productivity, reduce the use of chemicals and preserve the environment. However, more efficient methods for the use of inoculant are sought, especially in the stage of management by the producer, seeking to preserve the microorganism’s viability. The objective of this study was to evaluate the influence of inoculation methods with Azospirillum brasilense in the metabolism and the plant growth promotion in maize hybrid Formula VT®. In greenhouse, using as experimental unit polyethylene pots with 25 liter capacity, filled with Oxisol, were cultivated maize plants in function of different inoculation methods with the strains Abv5 and Abv% from the plant growth promoting bacteria A. brasilense. The inoculation methods consisted in the absence of inoculation (control), seed inoculation (during the seeding moment), foliar inoculation (in the phenological stage V4) and seed inoculation associated to foliar inoculation (during the seeding moment and in the phenological stage V4, respectively). The plant’s chlorophyll relative content was fortnightly evaluated. In the phenological stages V8, VT and R6 was measured the readings of the CO2 liquid assimilation rate in function of different flux densities of photosynthetically active photons. From these data it was determined the CO2 compensation point, the quantum yield and the maximum CO2 liquid assimilation. Still in the phenological stages V8, VT and R6, it was measured the number of leaves, aerial height, basal stem diameter, root volume, dry mass of leaves, stem+leaf sheath, roots and total dry mass, foliar area and foliar contents of nitrogen, phosphorus and potassium. Finally, in the phenological stage R6 it was evaluated the ear diameter, ear length, number of rows, number of kernels per row, dry mass of the reproductive structure, 100 grain mass, total grain mass and the grain content of nitrogen, phosphorus and potassium. Plants with inoculation via seed and seed inoculation associated to foliar inoculation stood out as to basal stem diameter, leaves dry mass, root volume and the contents of nitrogen, phosphorus and potassium. Still, the inoculated plants showed a differentiated capacity of transport of electrons and efficiency of CO2 liquid assimilation rate in relation to the control in all evaluated stages. The inoculation method via foliar influenced in the adaptative demand of the plants, reducing their development speed in the initial stage. The inoculation methods influenced in physiological, morphometric and nutritional parameters of the development of maize, however, they did not had any influence in the final yield. The plant development related to the growth promotion was positively influenced by the inoculation, varying according to the utilized method.O uso de bactérias promotoras de crescimento vegetal em milho destaca-se como alternativa para aumento de produtividade, redução de uso de químicos e preservação ambiental. Contudo, se busca-se métodos mais eficientes para uso do inoculante, em especial na etapa de manejo por parte do produtor, visando preservar a viabilidade do microrganismo. O objetivo do estudo foi avaliar a influência de métodos de inoculação com Azospirillum brasilense no metabolismo e promoção de crescimento vegetal em milho hibrido Formula VT®. Em casa de vegetação, utilizando como unidade experimental vasos de polietileno com capacidade para 25 L, preenchidos com LATOSSOLO VERMELHO Eutroférrico, foram cultivadas plantas de milho em função de diferentes métodos de inoculação com as estirpes Abv5 e Abv6 da bactéria promotora de crescimento vegetal A. brasilense. Os métodos de inoculação consistiram em ausência de inoculação (controle), inoculação via semente (no momento da semeadura), inoculação via foliar (no estádio fenológico V4) e inoculação via semente associada a foliar (no momento da semeadura e no estádio fenológico V4, respectivamente). Quinzenalmente as plantas foram avaliadas quando ao teor relativo de clorofila. Nos estádios fenológicos V8, VT e R6 foram realizadas as leituras de taxa de assimilação líquida de CO2 em função de diferentes densidades de fluxo de fótons fotossinteticamente ativos. Destes dados determinou-se o ponto de compensação de CO2, a eficiência quântica e a taxa de assimilação líquida de CO2 máxima. Ainda, nos estádios fenológicos V8, VT e R6, foram mensurados número de folhas, altura de parte aérea, diâmetro basal de colmo, volume de raiz, massa seca de folha, colmo+bainha, raiz e total, área foliar e teores foliares de nitrogênio, fósforo e potássio. Por fim, no estádio fenológico R6 avaliou-se diâmetro de espiga, comprimento de espiga, número de fileiras, número de grãos por fileira, massa seca de estrutura reprodutiva, massa de 100 grãos, massa total de grãos e teor de nitrogênio, fósforo e potássio dos grãos. Plantas inoculadas via semente e via semente associado a foliar destacaram-se quanto a diâmetro basal de colmo, massa seca foliar, volume de raiz, e aos teores de nitrogênio, fósforo e potássio. Ainda, plantas inoculadas apresentaram capacidade diferenciada de transporte de elétrons e eficiência de assimilação líquida de CO2, em relação ao controle, em todos os estádios analisados. O método de inoculação via foliar influenciou na demanda adaptativa das plantas, reduzindo a velocidade de desenvolvimento no estádio inicial. Os métodos de inoculação influenciaram em parâmetros fisiológicos, morfométricos e nutricionais do desenvolvimento do milho, contudo, não influenciaram na produtividade final. O desenvolvimento vegetal relacionado a promoção de crescimento foi influenciado positivamente pela inoculação, variando de acordo com o método utilizado.Submitted by Helena Bejio (helena.bejio@unioeste.br) on 2019-05-17T22:29:26Z No. of bitstreams: 2 Dissertação(3).pdf: 1642870 bytes, checksum: 45a6fc11a48340d149959f6152df6311 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2019-05-17T22:29:26Z (GMT). No. of bitstreams: 2 Dissertação(3).pdf: 1642870 bytes, checksum: 45a6fc11a48340d149959f6152df6311 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2019-02-25Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfpor-6392337873870130111500Universidade Estadual do Oeste do ParanáMarechal Cândido RondonPrograma de Pós-Graduação em AgronomiaUNIOESTEBrasilCentro de Ciências Agráriashttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessBactéria promotora de crescimento vegetalEficiência quânticaPonto de compensação de CO2Inoculação via foliarCIÊNCIAS AGRÁRIAS:AGRONOMIAMétodos de inoculação de Azospirillum brasilense e sua influência na promoção de crescimento do milhoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis5624066117035054290600600600-75855939502896689802075167498588264571reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALTauane_Brito_2019Tauane_Brito_2019application/pdf1642870http://tede.unioeste.br:8080/tede/bitstream/tede/4278/5/Tauane_Brito_201945a6fc11a48340d149959f6152df6311MD55CC-LICENSElicense_urllicense_urltext/plain; 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dc.title.por.fl_str_mv Métodos de inoculação de Azospirillum brasilense e sua influência na promoção de crescimento do milho
title Métodos de inoculação de Azospirillum brasilense e sua influência na promoção de crescimento do milho
spellingShingle Métodos de inoculação de Azospirillum brasilense e sua influência na promoção de crescimento do milho
Brito, Tauane Santos
Bactéria promotora de crescimento vegetal
Eficiência quântica
Ponto de compensação de CO2
Inoculação via foliar
CIÊNCIAS AGRÁRIAS:AGRONOMIA
title_short Métodos de inoculação de Azospirillum brasilense e sua influência na promoção de crescimento do milho
title_full Métodos de inoculação de Azospirillum brasilense e sua influência na promoção de crescimento do milho
title_fullStr Métodos de inoculação de Azospirillum brasilense e sua influência na promoção de crescimento do milho
title_full_unstemmed Métodos de inoculação de Azospirillum brasilense e sua influência na promoção de crescimento do milho
title_sort Métodos de inoculação de Azospirillum brasilense e sua influência na promoção de crescimento do milho
author Brito, Tauane Santos
author_facet Brito, Tauane Santos
author_role author
dc.contributor.advisor1.fl_str_mv Guimarães, Vandeir Francisco
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8187147768335662
dc.contributor.advisor-co1.fl_str_mv Braga, Gilberto Costa
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/5595085713178176
dc.contributor.advisor-co2.fl_str_mv Chaves, Elisiane Inês Dall’oglio
dc.contributor.advisor-co2Lattes.fl_str_mv http://lattes.cnpq.br/4193352042397738
dc.contributor.referee1.fl_str_mv Guimarães, Vandeir Francisco
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/8187147768335662
dc.contributor.referee2.fl_str_mv Ruppenthal, Viviane
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/6613787394484578
dc.contributor.referee3.fl_str_mv Nozaki, Márcia de Holanda
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/4976846609735019
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9985675185784615
dc.contributor.author.fl_str_mv Brito, Tauane Santos
contributor_str_mv Guimarães, Vandeir Francisco
Braga, Gilberto Costa
Chaves, Elisiane Inês Dall’oglio
Guimarães, Vandeir Francisco
Ruppenthal, Viviane
Nozaki, Márcia de Holanda
dc.subject.por.fl_str_mv Bactéria promotora de crescimento vegetal
Eficiência quântica
Ponto de compensação de CO2
Inoculação via foliar
topic Bactéria promotora de crescimento vegetal
Eficiência quântica
Ponto de compensação de CO2
Inoculação via foliar
CIÊNCIAS AGRÁRIAS:AGRONOMIA
dc.subject.cnpq.fl_str_mv CIÊNCIAS AGRÁRIAS:AGRONOMIA
description The use of plant growth promoting bacteria in maize stands out as an alternative to increase productivity, reduce the use of chemicals and preserve the environment. However, more efficient methods for the use of inoculant are sought, especially in the stage of management by the producer, seeking to preserve the microorganism’s viability. The objective of this study was to evaluate the influence of inoculation methods with Azospirillum brasilense in the metabolism and the plant growth promotion in maize hybrid Formula VT®. In greenhouse, using as experimental unit polyethylene pots with 25 liter capacity, filled with Oxisol, were cultivated maize plants in function of different inoculation methods with the strains Abv5 and Abv% from the plant growth promoting bacteria A. brasilense. The inoculation methods consisted in the absence of inoculation (control), seed inoculation (during the seeding moment), foliar inoculation (in the phenological stage V4) and seed inoculation associated to foliar inoculation (during the seeding moment and in the phenological stage V4, respectively). The plant’s chlorophyll relative content was fortnightly evaluated. In the phenological stages V8, VT and R6 was measured the readings of the CO2 liquid assimilation rate in function of different flux densities of photosynthetically active photons. From these data it was determined the CO2 compensation point, the quantum yield and the maximum CO2 liquid assimilation. Still in the phenological stages V8, VT and R6, it was measured the number of leaves, aerial height, basal stem diameter, root volume, dry mass of leaves, stem+leaf sheath, roots and total dry mass, foliar area and foliar contents of nitrogen, phosphorus and potassium. Finally, in the phenological stage R6 it was evaluated the ear diameter, ear length, number of rows, number of kernels per row, dry mass of the reproductive structure, 100 grain mass, total grain mass and the grain content of nitrogen, phosphorus and potassium. Plants with inoculation via seed and seed inoculation associated to foliar inoculation stood out as to basal stem diameter, leaves dry mass, root volume and the contents of nitrogen, phosphorus and potassium. Still, the inoculated plants showed a differentiated capacity of transport of electrons and efficiency of CO2 liquid assimilation rate in relation to the control in all evaluated stages. The inoculation method via foliar influenced in the adaptative demand of the plants, reducing their development speed in the initial stage. The inoculation methods influenced in physiological, morphometric and nutritional parameters of the development of maize, however, they did not had any influence in the final yield. The plant development related to the growth promotion was positively influenced by the inoculation, varying according to the utilized method.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-05-17T22:29:26Z
dc.date.issued.fl_str_mv 2019-02-25
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv BRITO, Tauane Santos. Métodos de inoculação de Azospirillum brasilense e sua influência na promoção de crescimento do milho. 2019. 75 f. Dissertação (Mestrado em Agronomia) - Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon, 2019.
dc.identifier.uri.fl_str_mv http://tede.unioeste.br/handle/tede/4278
identifier_str_mv BRITO, Tauane Santos. Métodos de inoculação de Azospirillum brasilense e sua influência na promoção de crescimento do milho. 2019. 75 f. Dissertação (Mestrado em Agronomia) - Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon, 2019.
url http://tede.unioeste.br/handle/tede/4278
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 5624066117035054290
dc.relation.confidence.fl_str_mv 600
600
600
dc.relation.department.fl_str_mv -7585593950289668980
dc.relation.sponsorship.fl_str_mv 2075167498588264571
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Marechal Cândido Rondon
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Agronomia
dc.publisher.initials.fl_str_mv UNIOESTE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Centro de Ciências Agrárias
publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Marechal Cândido Rondon
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTE
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