Efeitos da periodontite experimental sobre a morfofisiologia cognitiva do hipocampo
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UNIOESTE |
Texto Completo: | http://tede.unioeste.br/handle/tede/5634 |
Resumo: | Late evidence demonstrates a possible causal relationship between periodontitis and neurodegenerative diseases. Changes in the hippocampus can result in reduced cognitive functions, such as behaviors associated with memory and emotions. This study aimed to evaluate changes in the hippocampus and associated behaviors of rats with ligature-induced experimental periodontitis. Twenty 12-week-old male Wistar rats were used, divided into two groups: Control Group (CG) and Periodontal Disease Group (PDG). PDG received a bandage around the left and right first molars for 30 days, causing experimentally induced periodontitis. The animals of both groups were submitted to three behavioral tests (Open Field, Elevated Plus Maze, and Object Recognition) to assess locomotor and exploratory activities, anxiety, and memory, in two stages, being these: before and on the 27th day of the experiment, and 28 and 29 days after periodontitis induction. After 30 days, the rats underwent euthanasia and collection of the hippocampus (histological and immunological analysis) and hemimandibles (histological and radiographic analysis). The results were analyzed through the GraphPad software, using the Shapiro-Wilk test and the Student‟s t-test (p<0.05). Behavioral tests were analyzed using the Two-Way ANOVA test. PDG showed more considerable anxiety (Elevated Plus Maze Test), less habituation (Open Field Test), and reduced time to explore the new object (Object Recognition Test) compared to CG (p<0.05). Besides, PDG had a lower number of osteocytes and osteoblasts, and higher osteoclastic activity (p<0.05), as well as more significant alveolar bone loss morphometrically and radiographically (p<0.05) compared to CG. In the hippocampus, senile plaques were noted, in addition to positive beta-amyloid, tau, and CD68 markings on PDG. Therefore, it can be concluded that PD triggered the presence of senile plaques, beta-amyloid, tau, and CD68 positive markings, which, together, manifested an initial neuroinflammation process in these animals. |
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Nassar, Carlos Augustohttp://lattes.cnpq.br/3468024768828424Nassar, Carlos Augustohttp://lattes.cnpq.br/3468024768828424Ribeiro, Lucineia de Fatima Chaskohttp://lattes.cnpq.br/5546760712964930kerppers, Ivo Ilvanhttp://lattes.cnpq.br/2107257822885032http://lattes.cnpq.br/3436074367366927Gomes, Jossinelma Camargo2021-11-08T12:50:59Z2020-02-28GOMES, Jossinelma Camargo. Efeitos da periodontite experimental sobre a morfofisiologia cognitiva do hipocampo. 2020. 118 f. Dissertação (Mestrado em Biociências e Saúde) - Universidade Estadual do Oeste do Paraná, Cascavel - PR.http://tede.unioeste.br/handle/tede/5634Late evidence demonstrates a possible causal relationship between periodontitis and neurodegenerative diseases. Changes in the hippocampus can result in reduced cognitive functions, such as behaviors associated with memory and emotions. This study aimed to evaluate changes in the hippocampus and associated behaviors of rats with ligature-induced experimental periodontitis. Twenty 12-week-old male Wistar rats were used, divided into two groups: Control Group (CG) and Periodontal Disease Group (PDG). PDG received a bandage around the left and right first molars for 30 days, causing experimentally induced periodontitis. The animals of both groups were submitted to three behavioral tests (Open Field, Elevated Plus Maze, and Object Recognition) to assess locomotor and exploratory activities, anxiety, and memory, in two stages, being these: before and on the 27th day of the experiment, and 28 and 29 days after periodontitis induction. After 30 days, the rats underwent euthanasia and collection of the hippocampus (histological and immunological analysis) and hemimandibles (histological and radiographic analysis). The results were analyzed through the GraphPad software, using the Shapiro-Wilk test and the Student‟s t-test (p<0.05). Behavioral tests were analyzed using the Two-Way ANOVA test. PDG showed more considerable anxiety (Elevated Plus Maze Test), less habituation (Open Field Test), and reduced time to explore the new object (Object Recognition Test) compared to CG (p<0.05). Besides, PDG had a lower number of osteocytes and osteoblasts, and higher osteoclastic activity (p<0.05), as well as more significant alveolar bone loss morphometrically and radiographically (p<0.05) compared to CG. In the hippocampus, senile plaques were noted, in addition to positive beta-amyloid, tau, and CD68 markings on PDG. Therefore, it can be concluded that PD triggered the presence of senile plaques, beta-amyloid, tau, and CD68 positive markings, which, together, manifested an initial neuroinflammation process in these animals.Evidências recentes mostram uma possível relação causal entre periodontite e doenças neurodegenerativas. Alterações no hipocampo podem resultar em redução de funções cognitivas como comportamentos associados à memória e às emoções. O objetivo deste estudo foi avaliar alterações sobre o hipocampo e comportamentos associados de ratos com periodontite experimental induzida por ligadura. Foram utilizados 20 ratos Wistar machos com 12 semanas de idade, divididos em dois grupos: Grupo Controle (GC) e Grupo Doença Periodontal (GDP). O GDP recebeu uma ligadura ao redor dos primeiros molares inferiores direito e esquerdo por 30 dias, causando uma periodontite induzida experimentalmente. Os animais de ambos os grupos foram submetidos a três testes comportamentais: Campo Aberto / Open Field Labirinto em Cruz Elevado e Teste de Reconhecimento de Objetos; para avaliação de atividade exploratória, locomotora, ansiedade e de memória, em dois tempos, sendo estes: antes no 27, 28 e 29 dias após a indução da periodontite. Após 30 dias foi realizada a eutanásia, e, a coleta dos hipocampos (análise histológica e imunológica) e, também, das hemimandíbulas (análise histológica, histomorfométrica e radiográfica). Os resultados foram analisados pelo programa GraphPad, sendo realizado o teste de Shapiro-Wilk e o teste T de Student (p<0.05). Os testes comportamentais foram analisados pelo teste Two-Way ANOVA. O GDP apresentou maior ansiedade (Teste de Labirinto em Cruz Elevado), menor habituação (Teste Open Field) e redução do tempo de exploração do objeto novo (Teste de Reconhecimento de Objetos) em relação ao GC (p<0.05). Ainda o GDPapresentou menor número de osteócitos e osteoblastos e maior atividade osteoclástica (p<0.05), bem como maior perda óssea alveolar morfometricamente e radiograficamente (p<0.05) em relação ao GC. No hipocampo, notou-se presença de placas senis, além de marcações positivas de beta-amiloide, tau e CD68 no GDP. Logo se pode concluir que a DP desencadeou a presença de placas senis, marcações positivasde beta-amiloide, tau e CD68, os quais, em conjunto, manifestaram um processo de neuroinflamação inicial nesses animais.Submitted by Neusa Fagundes (neusa.fagundes@unioeste.br) on 2021-11-08T12:50:59Z No. of bitstreams: 2 Jossinelma_Gomes2020.pdf: 1840172 bytes, checksum: ee194c325aa07465e8ce39ff53e22103 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2021-11-08T12:50:59Z (GMT). No. of bitstreams: 2 Jossinelma_Gomes2020.pdf: 1840172 bytes, checksum: ee194c325aa07465e8ce39ff53e22103 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2020-02-28Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfpor6588633818200016417500Universidade Estadual do Oeste do ParanáCascavelPrograma de Pós-Graduação em Biociências e SaúdeUNIOESTEBrasilCentro de Ciências Biológicas e da Saúdehttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessPeriodontiteRatosHipocampoInflamaçãoPeriodontitisMouseHippocampusInflammationCIENCIAS BIOLOGICAS::BIOLOGIA GERALEfeitos da periodontite experimental sobre a morfofisiologia cognitiva do hipocampoEffects of experimental periodontitis the cognitive morphophysiology of the on the hippocampusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-82512614700830132786006006006001458059979463924370-16345593859312446972075167498588264571reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALJossinelma_Gomes2020.pdfJossinelma_Gomes2020.pdfapplication/pdf1840172http://tede.unioeste.br:8080/tede/bitstream/tede/5634/5/Jossinelma_Gomes2020.pdfee194c325aa07465e8ce39ff53e22103MD55CC-LICENSElicense_urllicense_urltext/plain; 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dc.title.por.fl_str_mv |
Efeitos da periodontite experimental sobre a morfofisiologia cognitiva do hipocampo |
dc.title.alternative.eng.fl_str_mv |
Effects of experimental periodontitis the cognitive morphophysiology of the on the hippocampus |
title |
Efeitos da periodontite experimental sobre a morfofisiologia cognitiva do hipocampo |
spellingShingle |
Efeitos da periodontite experimental sobre a morfofisiologia cognitiva do hipocampo Gomes, Jossinelma Camargo Periodontite Ratos Hipocampo Inflamação Periodontitis Mouse Hippocampus Inflammation CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Efeitos da periodontite experimental sobre a morfofisiologia cognitiva do hipocampo |
title_full |
Efeitos da periodontite experimental sobre a morfofisiologia cognitiva do hipocampo |
title_fullStr |
Efeitos da periodontite experimental sobre a morfofisiologia cognitiva do hipocampo |
title_full_unstemmed |
Efeitos da periodontite experimental sobre a morfofisiologia cognitiva do hipocampo |
title_sort |
Efeitos da periodontite experimental sobre a morfofisiologia cognitiva do hipocampo |
author |
Gomes, Jossinelma Camargo |
author_facet |
Gomes, Jossinelma Camargo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Nassar, Carlos Augusto |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3468024768828424 |
dc.contributor.referee1.fl_str_mv |
Nassar, Carlos Augusto |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/3468024768828424 |
dc.contributor.referee2.fl_str_mv |
Ribeiro, Lucineia de Fatima Chasko |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/5546760712964930 |
dc.contributor.referee3.fl_str_mv |
kerppers, Ivo Ilvan |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/2107257822885032 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3436074367366927 |
dc.contributor.author.fl_str_mv |
Gomes, Jossinelma Camargo |
contributor_str_mv |
Nassar, Carlos Augusto Nassar, Carlos Augusto Ribeiro, Lucineia de Fatima Chasko kerppers, Ivo Ilvan |
dc.subject.por.fl_str_mv |
Periodontite Ratos Hipocampo Inflamação |
topic |
Periodontite Ratos Hipocampo Inflamação Periodontitis Mouse Hippocampus Inflammation CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Periodontitis Mouse Hippocampus Inflammation |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
Late evidence demonstrates a possible causal relationship between periodontitis and neurodegenerative diseases. Changes in the hippocampus can result in reduced cognitive functions, such as behaviors associated with memory and emotions. This study aimed to evaluate changes in the hippocampus and associated behaviors of rats with ligature-induced experimental periodontitis. Twenty 12-week-old male Wistar rats were used, divided into two groups: Control Group (CG) and Periodontal Disease Group (PDG). PDG received a bandage around the left and right first molars for 30 days, causing experimentally induced periodontitis. The animals of both groups were submitted to three behavioral tests (Open Field, Elevated Plus Maze, and Object Recognition) to assess locomotor and exploratory activities, anxiety, and memory, in two stages, being these: before and on the 27th day of the experiment, and 28 and 29 days after periodontitis induction. After 30 days, the rats underwent euthanasia and collection of the hippocampus (histological and immunological analysis) and hemimandibles (histological and radiographic analysis). The results were analyzed through the GraphPad software, using the Shapiro-Wilk test and the Student‟s t-test (p<0.05). Behavioral tests were analyzed using the Two-Way ANOVA test. PDG showed more considerable anxiety (Elevated Plus Maze Test), less habituation (Open Field Test), and reduced time to explore the new object (Object Recognition Test) compared to CG (p<0.05). Besides, PDG had a lower number of osteocytes and osteoblasts, and higher osteoclastic activity (p<0.05), as well as more significant alveolar bone loss morphometrically and radiographically (p<0.05) compared to CG. In the hippocampus, senile plaques were noted, in addition to positive beta-amyloid, tau, and CD68 markings on PDG. Therefore, it can be concluded that PD triggered the presence of senile plaques, beta-amyloid, tau, and CD68 positive markings, which, together, manifested an initial neuroinflammation process in these animals. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-02-28 |
dc.date.accessioned.fl_str_mv |
2021-11-08T12:50:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GOMES, Jossinelma Camargo. Efeitos da periodontite experimental sobre a morfofisiologia cognitiva do hipocampo. 2020. 118 f. Dissertação (Mestrado em Biociências e Saúde) - Universidade Estadual do Oeste do Paraná, Cascavel - PR. |
dc.identifier.uri.fl_str_mv |
http://tede.unioeste.br/handle/tede/5634 |
identifier_str_mv |
GOMES, Jossinelma Camargo. Efeitos da periodontite experimental sobre a morfofisiologia cognitiva do hipocampo. 2020. 118 f. Dissertação (Mestrado em Biociências e Saúde) - Universidade Estadual do Oeste do Paraná, Cascavel - PR. |
url |
http://tede.unioeste.br/handle/tede/5634 |
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por |
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por |
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600 600 600 600 |
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Universidade Estadual do Oeste do Paraná Cascavel |
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Programa de Pós-Graduação em Biociências e Saúde |
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UNIOESTE |
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Brasil |
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Centro de Ciências Biológicas e da Saúde |
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Universidade Estadual do Oeste do Paraná Cascavel |
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