OPTIMIZATION OF GREEN PROPOLIS EXTRACTION BY ANTIFUNGAL ACTIVITY AND INCORPORATION IN CHITOSAN BIOFILM

• Autor(a) principal: Shigihara, Tabata Hana
• Data de Publicação: 2022
• Outros Autores: Figueiredo Pires, Elizabeth, de Assunção Justo, Diego, Mirante da Silva, Juliana Aparecida, Fiori Zara, Ricardo, Antunes Martin, Clayton, Cottica, Solange Maria, Shioji Tiuman, Tatiana
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista UniVap (online)
• Texto Completo: https://revista.univap.br/index.php/revistaunivap/article/view/4366
• topic: crude extract; experimental planning; minimum inhibitory concentration; Candida; Experimental planning; Microbiology; Biofilms; Propolis; extrato bruto; planejamento experimental; concentração inibitória mínima; Candida; Planejamento experimental; Microbiologia; Biofilmes; Própolis

The Brazilian propolis market has expanded greatly, leading the processing sector to pursue the best way to produce extracts with biological activities. The purpose of this research was to optimize the green propolis extraction by the antifungal activity and perform incorporation into a chitosan biofilm. The extracts were prepared using ethanol, acetone, ethyl acetate and isopropanol according to the three designs. The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were determined against Candida albicans, Candida tropicalis e Candida glabrata. From the MIC, the response surface analysis was carry out to find the optimal conditions for the extraction for each design. The optimized extracts were incorporate into chitosan biofilm that were subsequently evaluate for thickness, water vapor permeation, water solubility and anti-Candida activity. It was establish that all extracts tested showed antifungal activity against the three Candida species. The extract obtained with acetone/ethanol stood out because it presented the lowest values of MIC and MFC (0.76 – 2.50 mg/mL). It was observe that the optimal extraction points of each design were the blend of acetone/isopropanol, acetone/ethanol and ethyl acetate/isopropanol yet the biofilms incorporated with these extracts showed few differences in thickness, vapor permeability and water solubility in relation to the control design. The biofilm obtained by acetone/isopropanol displayed the higher activity anti-Candida. This results show the importance of optimizing propolis processes for the application and product development.