The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers

Detalhes bibliográficos
Autor(a) principal: Rodovalho, Cynara de Melo [UNESP]
Data de Publicação: 2014
Outros Autores: Lyra, Mariana Lucio [UNESP], Ferro, Milene [UNESP], Bacci, Maurício [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0097117
http://hdl.handle.net/11449/113352
Resumo: In this paper we describe the nearly complete mitochondrial genome of the leaf-cutter ant Atta laevigata, assembled using transcriptomic libraries from Sanger and Illumina next generation sequencing (NGS), and PCR products. This mitogenome was found to be very large (18,729 bp), given the presence of 30 non-coding intergenic spacers (IGS) spanning 3,808 bp. A portion of the putative control region remained unsequenced. The gene content and organization correspond to that inferred for the ancestral pancrustacea, except for two tRNA gene rearrangements that have been described previously in other ants. The IGS were highly variable in length and dispersed through the mitogenome. This pattern was also found for the other hymenopterans in particular for the monophyletic Apocrita. These spacers with unknown function may be valuable for characterizing genome evolution and distinguishing closely related species and individuals. NGS provided better coverage than Sanger sequencing, especially for tRNA and ribosomal subunit genes, thus facilitating efforts to fill in sequence gaps. The results obtained showed that data from transcriptomic libraries contain valuable information for assembling mitogenomes. The present data also provide a source of molecular markers that will be very important for improving our understanding of genomic evolutionary processes and phylogenetic relationships among hymenopterans.
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spelling The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic SpacersIn this paper we describe the nearly complete mitochondrial genome of the leaf-cutter ant Atta laevigata, assembled using transcriptomic libraries from Sanger and Illumina next generation sequencing (NGS), and PCR products. This mitogenome was found to be very large (18,729 bp), given the presence of 30 non-coding intergenic spacers (IGS) spanning 3,808 bp. A portion of the putative control region remained unsequenced. The gene content and organization correspond to that inferred for the ancestral pancrustacea, except for two tRNA gene rearrangements that have been described previously in other ants. The IGS were highly variable in length and dispersed through the mitogenome. This pattern was also found for the other hymenopterans in particular for the monophyletic Apocrita. These spacers with unknown function may be valuable for characterizing genome evolution and distinguishing closely related species and individuals. NGS provided better coverage than Sanger sequencing, especially for tRNA and ribosomal subunit genes, thus facilitating efforts to fill in sequence gaps. The results obtained showed that data from transcriptomic libraries contain valuable information for assembling mitogenomes. The present data also provide a source of molecular markers that will be very important for improving our understanding of genomic evolutionary processes and phylogenetic relationships among hymenopterans.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNESP Univ Estadual Paulista, Ctr Estudos Insetos Sociais, Sao Paulo, BrazilFIOCRUZ Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Rio De Janeiro, BrazilUNESP Univ Estadual Paulista, Dept Zool, Sao Paulo, BrazilUNESP Univ Estadual Paulista, Dept Bioquim & Microbiol, Sao Paulo, BrazilUNESP Univ Estadual Paulista, Ctr Estudos Insetos Sociais, Sao Paulo, BrazilUNESP Univ Estadual Paulista, Dept Zool, Sao Paulo, BrazilUNESP Univ Estadual Paulista, Dept Bioquim & Microbiol, Sao Paulo, BrazilFAPESP: 11/06367-8FAPESP: 11/50226-0CNPq: 311562/2012-4CNPq: 487639/2012-0Public Library ScienceUniversidade Estadual Paulista (Unesp)FIOCRUZ Fundacao Oswaldo CruzRodovalho, Cynara de Melo [UNESP]Lyra, Mariana Lucio [UNESP]Ferro, Milene [UNESP]Bacci, Maurício [UNESP]2014-12-03T13:11:38Z2014-12-03T13:11:38Z2014-05-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://dx.doi.org/10.1371/journal.pone.0097117Plos One. San Francisco: Public Library Science, v. 9, n. 5, 9 p., 2014.1932-6203http://hdl.handle.net/11449/11335210.1371/journal.pone.0097117WOS:000336857400055WOS000336857400055.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2024-04-11T14:57:11Zoai:repositorio.unesp.br:11449/113352Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:32:35.989785Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers
title The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers
spellingShingle The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers
Rodovalho, Cynara de Melo [UNESP]
title_short The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers
title_full The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers
title_fullStr The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers
title_full_unstemmed The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers
title_sort The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers
author Rodovalho, Cynara de Melo [UNESP]
author_facet Rodovalho, Cynara de Melo [UNESP]
Lyra, Mariana Lucio [UNESP]
Ferro, Milene [UNESP]
Bacci, Maurício [UNESP]
author_role author
author2 Lyra, Mariana Lucio [UNESP]
Ferro, Milene [UNESP]
Bacci, Maurício [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
FIOCRUZ Fundacao Oswaldo Cruz
dc.contributor.author.fl_str_mv Rodovalho, Cynara de Melo [UNESP]
Lyra, Mariana Lucio [UNESP]
Ferro, Milene [UNESP]
Bacci, Maurício [UNESP]
description In this paper we describe the nearly complete mitochondrial genome of the leaf-cutter ant Atta laevigata, assembled using transcriptomic libraries from Sanger and Illumina next generation sequencing (NGS), and PCR products. This mitogenome was found to be very large (18,729 bp), given the presence of 30 non-coding intergenic spacers (IGS) spanning 3,808 bp. A portion of the putative control region remained unsequenced. The gene content and organization correspond to that inferred for the ancestral pancrustacea, except for two tRNA gene rearrangements that have been described previously in other ants. The IGS were highly variable in length and dispersed through the mitogenome. This pattern was also found for the other hymenopterans in particular for the monophyletic Apocrita. These spacers with unknown function may be valuable for characterizing genome evolution and distinguishing closely related species and individuals. NGS provided better coverage than Sanger sequencing, especially for tRNA and ribosomal subunit genes, thus facilitating efforts to fill in sequence gaps. The results obtained showed that data from transcriptomic libraries contain valuable information for assembling mitogenomes. The present data also provide a source of molecular markers that will be very important for improving our understanding of genomic evolutionary processes and phylogenetic relationships among hymenopterans.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-03T13:11:38Z
2014-12-03T13:11:38Z
2014-05-14
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0097117
Plos One. San Francisco: Public Library Science, v. 9, n. 5, 9 p., 2014.
1932-6203
http://hdl.handle.net/11449/113352
10.1371/journal.pone.0097117
WOS:000336857400055
WOS000336857400055.pdf
url http://dx.doi.org/10.1371/journal.pone.0097117
http://hdl.handle.net/11449/113352
identifier_str_mv Plos One. San Francisco: Public Library Science, v. 9, n. 5, 9 p., 2014.
1932-6203
10.1371/journal.pone.0097117
WOS:000336857400055
WOS000336857400055.pdf
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
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reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
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reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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