The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0097117 http://hdl.handle.net/11449/113352 |
Resumo: | In this paper we describe the nearly complete mitochondrial genome of the leaf-cutter ant Atta laevigata, assembled using transcriptomic libraries from Sanger and Illumina next generation sequencing (NGS), and PCR products. This mitogenome was found to be very large (18,729 bp), given the presence of 30 non-coding intergenic spacers (IGS) spanning 3,808 bp. A portion of the putative control region remained unsequenced. The gene content and organization correspond to that inferred for the ancestral pancrustacea, except for two tRNA gene rearrangements that have been described previously in other ants. The IGS were highly variable in length and dispersed through the mitogenome. This pattern was also found for the other hymenopterans in particular for the monophyletic Apocrita. These spacers with unknown function may be valuable for characterizing genome evolution and distinguishing closely related species and individuals. NGS provided better coverage than Sanger sequencing, especially for tRNA and ribosomal subunit genes, thus facilitating efforts to fill in sequence gaps. The results obtained showed that data from transcriptomic libraries contain valuable information for assembling mitogenomes. The present data also provide a source of molecular markers that will be very important for improving our understanding of genomic evolutionary processes and phylogenetic relationships among hymenopterans. |
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The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic SpacersIn this paper we describe the nearly complete mitochondrial genome of the leaf-cutter ant Atta laevigata, assembled using transcriptomic libraries from Sanger and Illumina next generation sequencing (NGS), and PCR products. This mitogenome was found to be very large (18,729 bp), given the presence of 30 non-coding intergenic spacers (IGS) spanning 3,808 bp. A portion of the putative control region remained unsequenced. The gene content and organization correspond to that inferred for the ancestral pancrustacea, except for two tRNA gene rearrangements that have been described previously in other ants. The IGS were highly variable in length and dispersed through the mitogenome. This pattern was also found for the other hymenopterans in particular for the monophyletic Apocrita. These spacers with unknown function may be valuable for characterizing genome evolution and distinguishing closely related species and individuals. NGS provided better coverage than Sanger sequencing, especially for tRNA and ribosomal subunit genes, thus facilitating efforts to fill in sequence gaps. The results obtained showed that data from transcriptomic libraries contain valuable information for assembling mitogenomes. The present data also provide a source of molecular markers that will be very important for improving our understanding of genomic evolutionary processes and phylogenetic relationships among hymenopterans.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNESP Univ Estadual Paulista, Ctr Estudos Insetos Sociais, Sao Paulo, BrazilFIOCRUZ Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Rio De Janeiro, BrazilUNESP Univ Estadual Paulista, Dept Zool, Sao Paulo, BrazilUNESP Univ Estadual Paulista, Dept Bioquim & Microbiol, Sao Paulo, BrazilUNESP Univ Estadual Paulista, Ctr Estudos Insetos Sociais, Sao Paulo, BrazilUNESP Univ Estadual Paulista, Dept Zool, Sao Paulo, BrazilUNESP Univ Estadual Paulista, Dept Bioquim & Microbiol, Sao Paulo, BrazilFAPESP: 11/06367-8FAPESP: 11/50226-0CNPq: 311562/2012-4CNPq: 487639/2012-0Public Library ScienceUniversidade Estadual Paulista (Unesp)FIOCRUZ Fundacao Oswaldo CruzRodovalho, Cynara de Melo [UNESP]Lyra, Mariana Lucio [UNESP]Ferro, Milene [UNESP]Bacci, Maurício [UNESP]2014-12-03T13:11:38Z2014-12-03T13:11:38Z2014-05-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://dx.doi.org/10.1371/journal.pone.0097117Plos One. San Francisco: Public Library Science, v. 9, n. 5, 9 p., 2014.1932-6203http://hdl.handle.net/11449/11335210.1371/journal.pone.0097117WOS:000336857400055WOS000336857400055.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2024-04-11T14:57:11Zoai:repositorio.unesp.br:11449/113352Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:32:35.989785Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers |
title |
The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers |
spellingShingle |
The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers Rodovalho, Cynara de Melo [UNESP] |
title_short |
The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers |
title_full |
The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers |
title_fullStr |
The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers |
title_full_unstemmed |
The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers |
title_sort |
The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers |
author |
Rodovalho, Cynara de Melo [UNESP] |
author_facet |
Rodovalho, Cynara de Melo [UNESP] Lyra, Mariana Lucio [UNESP] Ferro, Milene [UNESP] Bacci, Maurício [UNESP] |
author_role |
author |
author2 |
Lyra, Mariana Lucio [UNESP] Ferro, Milene [UNESP] Bacci, Maurício [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) FIOCRUZ Fundacao Oswaldo Cruz |
dc.contributor.author.fl_str_mv |
Rodovalho, Cynara de Melo [UNESP] Lyra, Mariana Lucio [UNESP] Ferro, Milene [UNESP] Bacci, Maurício [UNESP] |
description |
In this paper we describe the nearly complete mitochondrial genome of the leaf-cutter ant Atta laevigata, assembled using transcriptomic libraries from Sanger and Illumina next generation sequencing (NGS), and PCR products. This mitogenome was found to be very large (18,729 bp), given the presence of 30 non-coding intergenic spacers (IGS) spanning 3,808 bp. A portion of the putative control region remained unsequenced. The gene content and organization correspond to that inferred for the ancestral pancrustacea, except for two tRNA gene rearrangements that have been described previously in other ants. The IGS were highly variable in length and dispersed through the mitogenome. This pattern was also found for the other hymenopterans in particular for the monophyletic Apocrita. These spacers with unknown function may be valuable for characterizing genome evolution and distinguishing closely related species and individuals. NGS provided better coverage than Sanger sequencing, especially for tRNA and ribosomal subunit genes, thus facilitating efforts to fill in sequence gaps. The results obtained showed that data from transcriptomic libraries contain valuable information for assembling mitogenomes. The present data also provide a source of molecular markers that will be very important for improving our understanding of genomic evolutionary processes and phylogenetic relationships among hymenopterans. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-03T13:11:38Z 2014-12-03T13:11:38Z 2014-05-14 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0097117 Plos One. San Francisco: Public Library Science, v. 9, n. 5, 9 p., 2014. 1932-6203 http://hdl.handle.net/11449/113352 10.1371/journal.pone.0097117 WOS:000336857400055 WOS000336857400055.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0097117 http://hdl.handle.net/11449/113352 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 9, n. 5, 9 p., 2014. 1932-6203 10.1371/journal.pone.0097117 WOS:000336857400055 WOS000336857400055.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLOS ONE 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128823078682624 |