Evaluation of a new method for (L+) lactic acid purification, using ethyl ether
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bcab.2020.101653 http://hdl.handle.net/11449/200519 |
Resumo: | The aim of the present study was to obtain high levels of fermentative L (+) lactic acid and find a better form of purification. The process was optimized by testing different compositions of the culture media, evaluating alternative carbon and nitrogen sources (agribusiness waste products), using inexpensive procedures and improve the production, recovery and purification of lactic acid from the fermented broth. Using 100 g/L crystallized sugar, 30 g/L yeast autolysate and NH4OH as the neutralizing agent, the production of L (+) lactic acid by Bacillus coagulans arr4 was 75.26 g/L in 24 h, with 2.76 g/L of residual sugar. The lactic acid purification and recovery experiments using double filtration with charcoal and Celite followed by a cation exchange column led to the economic recovery of lactic acid and the removal of substances and sugars. However, an additional purification step (extraction with ethyl ether) was needed for a microwave lactic acid polymerization reaction. Average recovery was 80%, with high purity. |
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Repositório Institucional da UNESP |
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Evaluation of a new method for (L+) lactic acid purification, using ethyl etherBacillusCeliteCharcoalEthyl etherLactic acidPurificationThe aim of the present study was to obtain high levels of fermentative L (+) lactic acid and find a better form of purification. The process was optimized by testing different compositions of the culture media, evaluating alternative carbon and nitrogen sources (agribusiness waste products), using inexpensive procedures and improve the production, recovery and purification of lactic acid from the fermented broth. Using 100 g/L crystallized sugar, 30 g/L yeast autolysate and NH4OH as the neutralizing agent, the production of L (+) lactic acid by Bacillus coagulans arr4 was 75.26 g/L in 24 h, with 2.76 g/L of residual sugar. The lactic acid purification and recovery experiments using double filtration with charcoal and Celite followed by a cation exchange column led to the economic recovery of lactic acid and the removal of substances and sugars. However, an additional purification step (extraction with ethyl ether) was needed for a microwave lactic acid polymerization reaction. Average recovery was 80%, with high purity.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (Unesp) Institute of Biosciences Department of Biochemistry and MicrobiologySão Paulo State University(Unesp) Institute for Research in BioenergySão Paulo State University (Unesp) Institute of Biosciences Department of Biochemistry and MicrobiologySão Paulo State University(Unesp) Institute for Research in BioenergyFAPESP: 2009/00142-4FAPESP: 2010/52416-8CNPq: 302935/2015-0Universidade Estadual Paulista (Unesp)Coelho, Luciana Fontes [UNESP]Sass, Daiane Cristina [UNESP]Avila Neto, Paulo Marcelo [UNESP]Contiero, Jonas [UNESP]2020-12-12T02:08:47Z2020-12-12T02:08:47Z2020-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bcab.2020.101653Biocatalysis and Agricultural Biotechnology, v. 26.1878-8181http://hdl.handle.net/11449/20051910.1016/j.bcab.2020.1016532-s2.0-85085499481Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiocatalysis and Agricultural Biotechnologyinfo:eu-repo/semantics/openAccess2021-10-23T14:33:52Zoai:repositorio.unesp.br:11449/200519Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:47:08.425812Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of a new method for (L+) lactic acid purification, using ethyl ether |
title |
Evaluation of a new method for (L+) lactic acid purification, using ethyl ether |
spellingShingle |
Evaluation of a new method for (L+) lactic acid purification, using ethyl ether Coelho, Luciana Fontes [UNESP] Bacillus Celite Charcoal Ethyl ether Lactic acid Purification |
title_short |
Evaluation of a new method for (L+) lactic acid purification, using ethyl ether |
title_full |
Evaluation of a new method for (L+) lactic acid purification, using ethyl ether |
title_fullStr |
Evaluation of a new method for (L+) lactic acid purification, using ethyl ether |
title_full_unstemmed |
Evaluation of a new method for (L+) lactic acid purification, using ethyl ether |
title_sort |
Evaluation of a new method for (L+) lactic acid purification, using ethyl ether |
author |
Coelho, Luciana Fontes [UNESP] |
author_facet |
Coelho, Luciana Fontes [UNESP] Sass, Daiane Cristina [UNESP] Avila Neto, Paulo Marcelo [UNESP] Contiero, Jonas [UNESP] |
author_role |
author |
author2 |
Sass, Daiane Cristina [UNESP] Avila Neto, Paulo Marcelo [UNESP] Contiero, Jonas [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Coelho, Luciana Fontes [UNESP] Sass, Daiane Cristina [UNESP] Avila Neto, Paulo Marcelo [UNESP] Contiero, Jonas [UNESP] |
dc.subject.por.fl_str_mv |
Bacillus Celite Charcoal Ethyl ether Lactic acid Purification |
topic |
Bacillus Celite Charcoal Ethyl ether Lactic acid Purification |
description |
The aim of the present study was to obtain high levels of fermentative L (+) lactic acid and find a better form of purification. The process was optimized by testing different compositions of the culture media, evaluating alternative carbon and nitrogen sources (agribusiness waste products), using inexpensive procedures and improve the production, recovery and purification of lactic acid from the fermented broth. Using 100 g/L crystallized sugar, 30 g/L yeast autolysate and NH4OH as the neutralizing agent, the production of L (+) lactic acid by Bacillus coagulans arr4 was 75.26 g/L in 24 h, with 2.76 g/L of residual sugar. The lactic acid purification and recovery experiments using double filtration with charcoal and Celite followed by a cation exchange column led to the economic recovery of lactic acid and the removal of substances and sugars. However, an additional purification step (extraction with ethyl ether) was needed for a microwave lactic acid polymerization reaction. Average recovery was 80%, with high purity. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:08:47Z 2020-12-12T02:08:47Z 2020-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bcab.2020.101653 Biocatalysis and Agricultural Biotechnology, v. 26. 1878-8181 http://hdl.handle.net/11449/200519 10.1016/j.bcab.2020.101653 2-s2.0-85085499481 |
url |
http://dx.doi.org/10.1016/j.bcab.2020.101653 http://hdl.handle.net/11449/200519 |
identifier_str_mv |
Biocatalysis and Agricultural Biotechnology, v. 26. 1878-8181 10.1016/j.bcab.2020.101653 2-s2.0-85085499481 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biocatalysis and Agricultural Biotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128416926400512 |