Synthesis of dental matrix proteins and viability of odontoblast-like cells irradiated with blue LED
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10103-016-1889-8 http://hdl.handle.net/11449/172714 |
Resumo: | To evaluate the effect of irradiation with light-emitting diode (LED; 455 nm) on the viability and synthesis of dentin matrix proteins by odontoblast-like cells, MDPC-23 cells were cultivated (104 cells/cm2) in 24-well culture plates. After 12 h incubation in Dulbecco’s modified Eagle’s medium (DMEM), the cells were submitted to nutritional restriction by means of reducing the concentration of fetal bovine serum (FBS) for an additional 12 h. Cells were irradiated one single time with one of the following energy densities (EDs): 0.5, 2, 4, 10, or 15 J/cm2 and irradiance fixed at 20 mW/cm2. Non-irradiated cells served as control. After 72 h, cells were evaluated with regard to viability (methylthiazol tetrazolium technique (MTT)), mineralization nodule (MN) formation, total protein (TP) production, alkaline phosphatase activity (ALP), and collagen synthesis (Sircol), n = 8. The data were submitted to Kruskal–Wallis and Mann–Whitney tests (p > 0.05). There was no statistical difference between the viability of cells irradiated or not (control), for all the EDs. However, an increase in TP was observed for all the EDs when compared with the control group. A reduced ALP activity was seen in all irradiated groups, except for the ED of 0.5 J/cm2, which did not differ from the control. There was no difference between the irradiated groups and control regarding collagen synthesis, with the exception of the ED of 10 J/cm2, which inhibited this cell function. Significant reduction in MN occurred only for the EDs of 0.5 and 2 J/cm2. The single irradiation with blue LED (455 nm), irradiance of 20 mW/cm2, and energy densities ranging from 0.5 to 15 J/cm2 exerted no effective biostimulatory capacity on odontoblast-like cells. |
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Synthesis of dental matrix proteins and viability of odontoblast-like cells irradiated with blue LEDLightLight-emitting diodeOdontoblastsPhototherapyTo evaluate the effect of irradiation with light-emitting diode (LED; 455 nm) on the viability and synthesis of dentin matrix proteins by odontoblast-like cells, MDPC-23 cells were cultivated (104 cells/cm2) in 24-well culture plates. After 12 h incubation in Dulbecco’s modified Eagle’s medium (DMEM), the cells were submitted to nutritional restriction by means of reducing the concentration of fetal bovine serum (FBS) for an additional 12 h. Cells were irradiated one single time with one of the following energy densities (EDs): 0.5, 2, 4, 10, or 15 J/cm2 and irradiance fixed at 20 mW/cm2. Non-irradiated cells served as control. After 72 h, cells were evaluated with regard to viability (methylthiazol tetrazolium technique (MTT)), mineralization nodule (MN) formation, total protein (TP) production, alkaline phosphatase activity (ALP), and collagen synthesis (Sircol), n = 8. The data were submitted to Kruskal–Wallis and Mann–Whitney tests (p > 0.05). There was no statistical difference between the viability of cells irradiated or not (control), for all the EDs. However, an increase in TP was observed for all the EDs when compared with the control group. A reduced ALP activity was seen in all irradiated groups, except for the ED of 0.5 J/cm2, which did not differ from the control. There was no difference between the irradiated groups and control regarding collagen synthesis, with the exception of the ED of 10 J/cm2, which inhibited this cell function. Significant reduction in MN occurred only for the EDs of 0.5 and 2 J/cm2. The single irradiation with blue LED (455 nm), irradiance of 20 mW/cm2, and energy densities ranging from 0.5 to 15 J/cm2 exerted no effective biostimulatory capacity on odontoblast-like cells.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Pediatric Dentistry and Orthodontics School of Dentistry at Araraquara Universidade Estadual Paulista-UNESP, Rua Humaitá, 1680Department of Physiology and Pathology School of Dentistry at Araraquara Universidade Estadual Paulista-UNESP, Rua Humaitá, 1680Departamento de Clínica Infantil Faculdade de Odontologia de Araraquara, Rua Humaitá, 1680Department of Pediatric Dentistry and Orthodontics School of Dentistry at Araraquara Universidade Estadual Paulista-UNESP, Rua Humaitá, 1680Department of Physiology and Pathology School of Dentistry at Araraquara Universidade Estadual Paulista-UNESP, Rua Humaitá, 1680FAPESP: 2012/08660-7CNPq: 303599/2014-6Universidade Estadual Paulista (Unesp)Faculdade de Odontologia de AraraquaraAlonso, Juliana Rosa Luiz [UNESP]Turrioni, Ana Paula Silveira [UNESP]Basso, Fernanda Gonçalves [UNESP]de Souza Costa, Carlos Alberto [UNESP]Hebling, Josimeri [UNESP]2018-12-11T17:01:53Z2018-12-11T17:01:53Z2016-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article523-530application/pdfhttp://dx.doi.org/10.1007/s10103-016-1889-8Lasers in Medical Science, v. 31, n. 3, p. 523-530, 2016.1435-604X0268-8921http://hdl.handle.net/11449/17271410.1007/s10103-016-1889-82-s2.0-849612088502-s2.0-84961208850.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Science0,713info:eu-repo/semantics/openAccess2023-12-20T06:26:07Zoai:repositorio.unesp.br:11449/172714Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:51:23.125213Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Synthesis of dental matrix proteins and viability of odontoblast-like cells irradiated with blue LED |
title |
Synthesis of dental matrix proteins and viability of odontoblast-like cells irradiated with blue LED |
spellingShingle |
Synthesis of dental matrix proteins and viability of odontoblast-like cells irradiated with blue LED Alonso, Juliana Rosa Luiz [UNESP] Light Light-emitting diode Odontoblasts Phototherapy |
title_short |
Synthesis of dental matrix proteins and viability of odontoblast-like cells irradiated with blue LED |
title_full |
Synthesis of dental matrix proteins and viability of odontoblast-like cells irradiated with blue LED |
title_fullStr |
Synthesis of dental matrix proteins and viability of odontoblast-like cells irradiated with blue LED |
title_full_unstemmed |
Synthesis of dental matrix proteins and viability of odontoblast-like cells irradiated with blue LED |
title_sort |
Synthesis of dental matrix proteins and viability of odontoblast-like cells irradiated with blue LED |
author |
Alonso, Juliana Rosa Luiz [UNESP] |
author_facet |
Alonso, Juliana Rosa Luiz [UNESP] Turrioni, Ana Paula Silveira [UNESP] Basso, Fernanda Gonçalves [UNESP] de Souza Costa, Carlos Alberto [UNESP] Hebling, Josimeri [UNESP] |
author_role |
author |
author2 |
Turrioni, Ana Paula Silveira [UNESP] Basso, Fernanda Gonçalves [UNESP] de Souza Costa, Carlos Alberto [UNESP] Hebling, Josimeri [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Faculdade de Odontologia de Araraquara |
dc.contributor.author.fl_str_mv |
Alonso, Juliana Rosa Luiz [UNESP] Turrioni, Ana Paula Silveira [UNESP] Basso, Fernanda Gonçalves [UNESP] de Souza Costa, Carlos Alberto [UNESP] Hebling, Josimeri [UNESP] |
dc.subject.por.fl_str_mv |
Light Light-emitting diode Odontoblasts Phototherapy |
topic |
Light Light-emitting diode Odontoblasts Phototherapy |
description |
To evaluate the effect of irradiation with light-emitting diode (LED; 455 nm) on the viability and synthesis of dentin matrix proteins by odontoblast-like cells, MDPC-23 cells were cultivated (104 cells/cm2) in 24-well culture plates. After 12 h incubation in Dulbecco’s modified Eagle’s medium (DMEM), the cells were submitted to nutritional restriction by means of reducing the concentration of fetal bovine serum (FBS) for an additional 12 h. Cells were irradiated one single time with one of the following energy densities (EDs): 0.5, 2, 4, 10, or 15 J/cm2 and irradiance fixed at 20 mW/cm2. Non-irradiated cells served as control. After 72 h, cells were evaluated with regard to viability (methylthiazol tetrazolium technique (MTT)), mineralization nodule (MN) formation, total protein (TP) production, alkaline phosphatase activity (ALP), and collagen synthesis (Sircol), n = 8. The data were submitted to Kruskal–Wallis and Mann–Whitney tests (p > 0.05). There was no statistical difference between the viability of cells irradiated or not (control), for all the EDs. However, an increase in TP was observed for all the EDs when compared with the control group. A reduced ALP activity was seen in all irradiated groups, except for the ED of 0.5 J/cm2, which did not differ from the control. There was no difference between the irradiated groups and control regarding collagen synthesis, with the exception of the ED of 10 J/cm2, which inhibited this cell function. Significant reduction in MN occurred only for the EDs of 0.5 and 2 J/cm2. The single irradiation with blue LED (455 nm), irradiance of 20 mW/cm2, and energy densities ranging from 0.5 to 15 J/cm2 exerted no effective biostimulatory capacity on odontoblast-like cells. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04-01 2018-12-11T17:01:53Z 2018-12-11T17:01:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10103-016-1889-8 Lasers in Medical Science, v. 31, n. 3, p. 523-530, 2016. 1435-604X 0268-8921 http://hdl.handle.net/11449/172714 10.1007/s10103-016-1889-8 2-s2.0-84961208850 2-s2.0-84961208850.pdf |
url |
http://dx.doi.org/10.1007/s10103-016-1889-8 http://hdl.handle.net/11449/172714 |
identifier_str_mv |
Lasers in Medical Science, v. 31, n. 3, p. 523-530, 2016. 1435-604X 0268-8921 10.1007/s10103-016-1889-8 2-s2.0-84961208850 2-s2.0-84961208850.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lasers in Medical Science 0,713 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
523-530 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129258357260288 |