The role of testosterone in the respiratory and thermal responses to hypoxia and hypercapnia in rats
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1530/JOE-20-0257 http://hdl.handle.net/11449/209501 |
Resumo: | Many diseases of the respiratory system occur differently in males and females, indicating a possible role of gonadal hormones in respiratory control. We hypothesized that testosterone (T) is important for the ventilatory chemosensitivity responses in males. To test this hypothesis, we evaluated ventilation (<(V)over dot>(E)), metabolic rate and body temperature (T-b) under normoxia/normocapnia, hypercapnia and hypoxia in orchiectomized (ORX), ORX with testosterone replacement (ORX + T) or flutamide (FL, androgen receptor blocker)-treated rats. We also performed immunohistochemistry to evaluate the presence of androgen receptor (AR) in the carotid body ( CB) of intact males. Orchiectomy promoted a reduction <(V)over dot>(E) and ventilatory equivalent (<(V)over dot>(E)/<(V)over dot>O-2) under room-air conditions, which was restored with testosterone treatment. Moreover, during hypoxia or hypercapnia, animals that received testosterone replacement had a higher <(V)over dot>(E) and <(V)over dot>(E)/<(V)over dot>O-2 than control and ORX, without changes in metabolic and thermal variables. Flutamide decreased the hypoxic ventilatory response without changing the CO2-drive to breathe, suggesting that the testosterone effect on hypercapnic hyperventilation does not appear to involve the AR. We also determined the presence of AR in the CB of intact animals. Our findings demonstrate that testosterone seems to be important for maintaining resting <(V)over dot>(E) in males. In addition, the influence of testosterone on <(V)over dot>(E), either during resting conditions or under hypoxia and hypercapnia, seems to be a direct and specific effect, as no changes in metabolic rate or T-b were observed during any treatment. Finally, a putative site of testosterone action during hypoxia is the CB, since we detected the presence of AR in this structure. |
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The role of testosterone in the respiratory and thermal responses to hypoxia and hypercapnia in ratsgonadal hormoneandrogensbreathingchemosensitivityMany diseases of the respiratory system occur differently in males and females, indicating a possible role of gonadal hormones in respiratory control. We hypothesized that testosterone (T) is important for the ventilatory chemosensitivity responses in males. To test this hypothesis, we evaluated ventilation (<(V)over dot>(E)), metabolic rate and body temperature (T-b) under normoxia/normocapnia, hypercapnia and hypoxia in orchiectomized (ORX), ORX with testosterone replacement (ORX + T) or flutamide (FL, androgen receptor blocker)-treated rats. We also performed immunohistochemistry to evaluate the presence of androgen receptor (AR) in the carotid body ( CB) of intact males. Orchiectomy promoted a reduction <(V)over dot>(E) and ventilatory equivalent (<(V)over dot>(E)/<(V)over dot>O-2) under room-air conditions, which was restored with testosterone treatment. Moreover, during hypoxia or hypercapnia, animals that received testosterone replacement had a higher <(V)over dot>(E) and <(V)over dot>(E)/<(V)over dot>O-2 than control and ORX, without changes in metabolic and thermal variables. Flutamide decreased the hypoxic ventilatory response without changing the CO2-drive to breathe, suggesting that the testosterone effect on hypercapnic hyperventilation does not appear to involve the AR. We also determined the presence of AR in the CB of intact animals. Our findings demonstrate that testosterone seems to be important for maintaining resting <(V)over dot>(E) in males. In addition, the influence of testosterone on <(V)over dot>(E), either during resting conditions or under hypoxia and hypercapnia, seems to be a direct and specific effect, as no changes in metabolic rate or T-b were observed during any treatment. Finally, a putative site of testosterone action during hypoxia is the CB, since we detected the presence of AR in this structure.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sao Paulo State Univ, Fac Agr & Veterinarian Sci, Dept Anim Morphol & Physiol, Jaboticabal, SP, BrazilSt Michaels Hosp, Brain & Breathing Sci Lab, Keenan Res Ctr Biomed Sci, Toronto, ON, CanadaFed Univ Minas Gerais UFMG, Inst Biol Sci, Dept Physiol & Biophys, Belo Horizonte, MG, BrazilSao Paulo State Univ, Fac Agr & Veterinarian Sci, Dept Anim Morphol & Physiol, Jaboticabal, SP, BrazilFAPESP: 2019/09469-8CNPq: CNPq -407490/2018-3FAPESP: 2016/04276-9CNPq: 140715/2015-0Bioscientifica LtdUniversidade Estadual Paulista (Unesp)St Michaels HospUniversidade Federal de Minas Gerais (UFMG)Marques, Danuzia A. [UNESP]Patrone, Luis Gustavo A. [UNESP]Scarpellini, Carolina S. [UNESP]Bicego, Kenia C. [UNESP]Szawka, Raphael E.Gargaglioni, Luciane H. [UNESP]2021-06-25T12:20:30Z2021-06-25T12:20:30Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article101-114http://dx.doi.org/10.1530/JOE-20-0257Journal Of Endocrinology. Bristol: Bioscientifica Ltd, v. 247, n. 1, p. 101-114, 2020.0022-0795http://hdl.handle.net/11449/20950110.1530/JOE-20-0257WOS:000574819900011Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Endocrinologyinfo:eu-repo/semantics/openAccess2024-06-06T18:41:18Zoai:repositorio.unesp.br:11449/209501Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:56:27.703174Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The role of testosterone in the respiratory and thermal responses to hypoxia and hypercapnia in rats |
title |
The role of testosterone in the respiratory and thermal responses to hypoxia and hypercapnia in rats |
spellingShingle |
The role of testosterone in the respiratory and thermal responses to hypoxia and hypercapnia in rats Marques, Danuzia A. [UNESP] gonadal hormone androgens breathing chemosensitivity |
title_short |
The role of testosterone in the respiratory and thermal responses to hypoxia and hypercapnia in rats |
title_full |
The role of testosterone in the respiratory and thermal responses to hypoxia and hypercapnia in rats |
title_fullStr |
The role of testosterone in the respiratory and thermal responses to hypoxia and hypercapnia in rats |
title_full_unstemmed |
The role of testosterone in the respiratory and thermal responses to hypoxia and hypercapnia in rats |
title_sort |
The role of testosterone in the respiratory and thermal responses to hypoxia and hypercapnia in rats |
author |
Marques, Danuzia A. [UNESP] |
author_facet |
Marques, Danuzia A. [UNESP] Patrone, Luis Gustavo A. [UNESP] Scarpellini, Carolina S. [UNESP] Bicego, Kenia C. [UNESP] Szawka, Raphael E. Gargaglioni, Luciane H. [UNESP] |
author_role |
author |
author2 |
Patrone, Luis Gustavo A. [UNESP] Scarpellini, Carolina S. [UNESP] Bicego, Kenia C. [UNESP] Szawka, Raphael E. Gargaglioni, Luciane H. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) St Michaels Hosp Universidade Federal de Minas Gerais (UFMG) |
dc.contributor.author.fl_str_mv |
Marques, Danuzia A. [UNESP] Patrone, Luis Gustavo A. [UNESP] Scarpellini, Carolina S. [UNESP] Bicego, Kenia C. [UNESP] Szawka, Raphael E. Gargaglioni, Luciane H. [UNESP] |
dc.subject.por.fl_str_mv |
gonadal hormone androgens breathing chemosensitivity |
topic |
gonadal hormone androgens breathing chemosensitivity |
description |
Many diseases of the respiratory system occur differently in males and females, indicating a possible role of gonadal hormones in respiratory control. We hypothesized that testosterone (T) is important for the ventilatory chemosensitivity responses in males. To test this hypothesis, we evaluated ventilation (<(V)over dot>(E)), metabolic rate and body temperature (T-b) under normoxia/normocapnia, hypercapnia and hypoxia in orchiectomized (ORX), ORX with testosterone replacement (ORX + T) or flutamide (FL, androgen receptor blocker)-treated rats. We also performed immunohistochemistry to evaluate the presence of androgen receptor (AR) in the carotid body ( CB) of intact males. Orchiectomy promoted a reduction <(V)over dot>(E) and ventilatory equivalent (<(V)over dot>(E)/<(V)over dot>O-2) under room-air conditions, which was restored with testosterone treatment. Moreover, during hypoxia or hypercapnia, animals that received testosterone replacement had a higher <(V)over dot>(E) and <(V)over dot>(E)/<(V)over dot>O-2 than control and ORX, without changes in metabolic and thermal variables. Flutamide decreased the hypoxic ventilatory response without changing the CO2-drive to breathe, suggesting that the testosterone effect on hypercapnic hyperventilation does not appear to involve the AR. We also determined the presence of AR in the CB of intact animals. Our findings demonstrate that testosterone seems to be important for maintaining resting <(V)over dot>(E) in males. In addition, the influence of testosterone on <(V)over dot>(E), either during resting conditions or under hypoxia and hypercapnia, seems to be a direct and specific effect, as no changes in metabolic rate or T-b were observed during any treatment. Finally, a putative site of testosterone action during hypoxia is the CB, since we detected the presence of AR in this structure. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-01 2021-06-25T12:20:30Z 2021-06-25T12:20:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1530/JOE-20-0257 Journal Of Endocrinology. Bristol: Bioscientifica Ltd, v. 247, n. 1, p. 101-114, 2020. 0022-0795 http://hdl.handle.net/11449/209501 10.1530/JOE-20-0257 WOS:000574819900011 |
url |
http://dx.doi.org/10.1530/JOE-20-0257 http://hdl.handle.net/11449/209501 |
identifier_str_mv |
Journal Of Endocrinology. Bristol: Bioscientifica Ltd, v. 247, n. 1, p. 101-114, 2020. 0022-0795 10.1530/JOE-20-0257 WOS:000574819900011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Endocrinology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
101-114 |
dc.publisher.none.fl_str_mv |
Bioscientifica Ltd |
publisher.none.fl_str_mv |
Bioscientifica Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128439334469632 |