Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10103-018-2574-x http://hdl.handle.net/11449/171190 |
Resumo: | This study aimed to analyze the effects of laser irradiation on the membrane integrity and viability of stem cells from human exfoliated deciduous teeth (SHED) that were kept in serum starvation. Nutritional deficit was used to mimic the cellular stress conditions of SHED isolation for regenerative dental approaches, where laser therapy could be beneficial. SHED were cultured under serum starvation (MEMα + 1%FBS) for 1 or 24 h pre-irradiation (protocols A and B, respectively). Then, cells received low-level laser therapy (LLLT; 660 nm) at 2.5 J/cm2 (0.10 W; groups I and V), 5.0 J/cm2 (0.20 W; groups II and VI), 7.5 J/cm2 (0.30 W; groups III and VII), or remained non-irradiated (groups IV and VIII). During irradiation, cells were maintained in 1% FBS (groups I–IV) or 10% FBS (normal culture conditions; groups V–VIII). Membrane integrity was evaluated by quantifying lactate dehydrogenase (LDH) release (immediately after irradiation), and cell viability was assessed by the MTT assay (24, 48, and 72 h post-irradiation). Serum starvation did not alter LDH release by non-irradiated SHED, while LDH release decreased significantly in groups irradiated in 1% FBS (I and III), but not in groups irradiated in 10% FBS (V–VII), regardless the pre-irradiation conditions (protocols A/B). Cell viability was significantly higher 24 h after irradiation, in most protocol A groups. In contrast, cell viability remained mostly unaltered in protocol B groups. LLLT contributed to maintain membrane integrity in SHED subjected to nutritional deficit before and during irradiation with 0.10 or 0.30 W. Short serum starvation before irradiation improved SHED viability at 24 h post-irradiation. |
id |
UNSP_0f4f86cab2aaecb375b088a8dd8a54c0 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/171190 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficitCell culture techniquesCell survivalL-lactate dehydrogenaseLow-level light therapyStem cellsThis study aimed to analyze the effects of laser irradiation on the membrane integrity and viability of stem cells from human exfoliated deciduous teeth (SHED) that were kept in serum starvation. Nutritional deficit was used to mimic the cellular stress conditions of SHED isolation for regenerative dental approaches, where laser therapy could be beneficial. SHED were cultured under serum starvation (MEMα + 1%FBS) for 1 or 24 h pre-irradiation (protocols A and B, respectively). Then, cells received low-level laser therapy (LLLT; 660 nm) at 2.5 J/cm2 (0.10 W; groups I and V), 5.0 J/cm2 (0.20 W; groups II and VI), 7.5 J/cm2 (0.30 W; groups III and VII), or remained non-irradiated (groups IV and VIII). During irradiation, cells were maintained in 1% FBS (groups I–IV) or 10% FBS (normal culture conditions; groups V–VIII). Membrane integrity was evaluated by quantifying lactate dehydrogenase (LDH) release (immediately after irradiation), and cell viability was assessed by the MTT assay (24, 48, and 72 h post-irradiation). Serum starvation did not alter LDH release by non-irradiated SHED, while LDH release decreased significantly in groups irradiated in 1% FBS (I and III), but not in groups irradiated in 10% FBS (V–VII), regardless the pre-irradiation conditions (protocols A/B). Cell viability was significantly higher 24 h after irradiation, in most protocol A groups. In contrast, cell viability remained mostly unaltered in protocol B groups. LLLT contributed to maintain membrane integrity in SHED subjected to nutritional deficit before and during irradiation with 0.10 or 0.30 W. Short serum starvation before irradiation improved SHED viability at 24 h post-irradiation.Department of Clinics and Surgery School of Dentistry Federal University of Alfenas (UNIFAL-MG), Rua Gabriel Monteiro da Silva, 700.Department of Pediatric Dentistry Orthodontics and Public Health Bauru School of Dentistry University of São Paulo (USP)Department of Pediatric Dentistry and Public Health School of Dentistry São Paulo State University (UNESP)Department of Pediatric Dentistry School of Dentistry José do Rosário Vellano University (UNIFENAS)Department of Pediatric Dentistry and Public Health School of Dentistry São Paulo State University (UNESP)Federal University of Alfenas (UNIFAL-MG)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)José do Rosário Vellano University (UNIFENAS)da Silva, Paula Corrêa SilveiraMarques, Nelson PereiraFarina, Marcella TassiOliveira, Thais MarchiniDuque, Cristiane [UNESP]Marques, Nádia Carolina TeixeiraSakai, Vivien Thiemy2018-12-11T16:54:19Z2018-12-11T16:54:19Z2018-07-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-7application/pdfhttp://dx.doi.org/10.1007/s10103-018-2574-xLasers in Medical Science, p. 1-7.1435-604X0268-8921http://hdl.handle.net/11449/17119010.1007/s10103-018-2574-x2-s2.0-850495879462-s2.0-85049587946.pdf56518745094936170000-0002-2575-279XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Science0,713info:eu-repo/semantics/openAccess2023-12-04T06:12:00Zoai:repositorio.unesp.br:11449/171190Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:27:02.513217Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit |
title |
Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit |
spellingShingle |
Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit da Silva, Paula Corrêa Silveira Cell culture techniques Cell survival L-lactate dehydrogenase Low-level light therapy Stem cells |
title_short |
Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit |
title_full |
Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit |
title_fullStr |
Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit |
title_full_unstemmed |
Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit |
title_sort |
Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit |
author |
da Silva, Paula Corrêa Silveira |
author_facet |
da Silva, Paula Corrêa Silveira Marques, Nelson Pereira Farina, Marcella Tassi Oliveira, Thais Marchini Duque, Cristiane [UNESP] Marques, Nádia Carolina Teixeira Sakai, Vivien Thiemy |
author_role |
author |
author2 |
Marques, Nelson Pereira Farina, Marcella Tassi Oliveira, Thais Marchini Duque, Cristiane [UNESP] Marques, Nádia Carolina Teixeira Sakai, Vivien Thiemy |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Alfenas (UNIFAL-MG) Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) José do Rosário Vellano University (UNIFENAS) |
dc.contributor.author.fl_str_mv |
da Silva, Paula Corrêa Silveira Marques, Nelson Pereira Farina, Marcella Tassi Oliveira, Thais Marchini Duque, Cristiane [UNESP] Marques, Nádia Carolina Teixeira Sakai, Vivien Thiemy |
dc.subject.por.fl_str_mv |
Cell culture techniques Cell survival L-lactate dehydrogenase Low-level light therapy Stem cells |
topic |
Cell culture techniques Cell survival L-lactate dehydrogenase Low-level light therapy Stem cells |
description |
This study aimed to analyze the effects of laser irradiation on the membrane integrity and viability of stem cells from human exfoliated deciduous teeth (SHED) that were kept in serum starvation. Nutritional deficit was used to mimic the cellular stress conditions of SHED isolation for regenerative dental approaches, where laser therapy could be beneficial. SHED were cultured under serum starvation (MEMα + 1%FBS) for 1 or 24 h pre-irradiation (protocols A and B, respectively). Then, cells received low-level laser therapy (LLLT; 660 nm) at 2.5 J/cm2 (0.10 W; groups I and V), 5.0 J/cm2 (0.20 W; groups II and VI), 7.5 J/cm2 (0.30 W; groups III and VII), or remained non-irradiated (groups IV and VIII). During irradiation, cells were maintained in 1% FBS (groups I–IV) or 10% FBS (normal culture conditions; groups V–VIII). Membrane integrity was evaluated by quantifying lactate dehydrogenase (LDH) release (immediately after irradiation), and cell viability was assessed by the MTT assay (24, 48, and 72 h post-irradiation). Serum starvation did not alter LDH release by non-irradiated SHED, while LDH release decreased significantly in groups irradiated in 1% FBS (I and III), but not in groups irradiated in 10% FBS (V–VII), regardless the pre-irradiation conditions (protocols A/B). Cell viability was significantly higher 24 h after irradiation, in most protocol A groups. In contrast, cell viability remained mostly unaltered in protocol B groups. LLLT contributed to maintain membrane integrity in SHED subjected to nutritional deficit before and during irradiation with 0.10 or 0.30 W. Short serum starvation before irradiation improved SHED viability at 24 h post-irradiation. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:54:19Z 2018-12-11T16:54:19Z 2018-07-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10103-018-2574-x Lasers in Medical Science, p. 1-7. 1435-604X 0268-8921 http://hdl.handle.net/11449/171190 10.1007/s10103-018-2574-x 2-s2.0-85049587946 2-s2.0-85049587946.pdf 5651874509493617 0000-0002-2575-279X |
url |
http://dx.doi.org/10.1007/s10103-018-2574-x http://hdl.handle.net/11449/171190 |
identifier_str_mv |
Lasers in Medical Science, p. 1-7. 1435-604X 0268-8921 10.1007/s10103-018-2574-x 2-s2.0-85049587946 2-s2.0-85049587946.pdf 5651874509493617 0000-0002-2575-279X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lasers in Medical Science 0,713 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-7 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129070703050752 |