The use of an integrative approach to improve accuracy of species identification and detection of new species in studies of stream fish diversity
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10709-021-00118-6 http://hdl.handle.net/11449/206249 |
Resumo: | In this study, we made an inventory of the stream and headwater ichthyofauna of the left bank of the Itaipu Dam Reservoir, located in the lower part of the Upper Paraná River basin, using an integrative approach of molecular and morphological data. The area is located in the western portion of the Paraná state in Brazil, in an area of about 8,000 km2 highly impacted by deforestation and intensive agriculture. For taxonomic identification of species, we used an identification key combined with the DNA barcoding approach. We found 48 species belonging to six orders, 13 families, and 37 genera. The Siluriformes and Characiformes were the most representative orders (75%) and the Characidae was the most representative family (20.8%). Nine species prevailed in this region, making up to 86% of all specimens collected. The integrative approach proved to be useful by allowing the unambiguous identification of all species, including those cases in which morphological characters were not conclusive for species identification, cases of cryptic species, and species with high morphological plasticity. In addition, the integrative approach highlighted six to 13 new putative species depending on the approach considered. Our study provides a relevant contribution to the knowledge of fish diversity in a poorly studied area of the Paraná River basin. We showed that the use of an integrative approach in inventory studies improves species identification and the discovery of new, cryptic, and overlooked species, being a powerful and necessary tool to quantify biodiversity. |
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The use of an integrative approach to improve accuracy of species identification and detection of new species in studies of stream fish diversityChecklistConservationDNA barcodingFish distributionFishesParaná River basinIn this study, we made an inventory of the stream and headwater ichthyofauna of the left bank of the Itaipu Dam Reservoir, located in the lower part of the Upper Paraná River basin, using an integrative approach of molecular and morphological data. The area is located in the western portion of the Paraná state in Brazil, in an area of about 8,000 km2 highly impacted by deforestation and intensive agriculture. For taxonomic identification of species, we used an identification key combined with the DNA barcoding approach. We found 48 species belonging to six orders, 13 families, and 37 genera. The Siluriformes and Characiformes were the most representative orders (75%) and the Characidae was the most representative family (20.8%). Nine species prevailed in this region, making up to 86% of all specimens collected. The integrative approach proved to be useful by allowing the unambiguous identification of all species, including those cases in which morphological characters were not conclusive for species identification, cases of cryptic species, and species with high morphological plasticity. In addition, the integrative approach highlighted six to 13 new putative species depending on the approach considered. Our study provides a relevant contribution to the knowledge of fish diversity in a poorly studied area of the Paraná River basin. We showed that the use of an integrative approach in inventory studies improves species identification and the discovery of new, cryptic, and overlooked species, being a powerful and necessary tool to quantify biodiversity.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Laboratório de Biodiversidade e Ecologia Molecular Instituto Latino-Americano de Ciências da Vida e da Natureza – ILACVN Universidade Federal da Integração Latino-Americana – UNILA, Av. Tarquínio Joslin dos Santos, 1000Departamento de Biologia estrutural e Funcional Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho - UNESPDepartamento de Biologia estrutural e Funcional Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho - UNESPFAPESP: 2014/26508-3FAPESP: 2016/09204-6FAPESP: 2018/20610-1CNPq: 306054/2006-0Universidade Federal da Integração Latino-Americana – UNILAUniversidade Estadual Paulista (Unesp)Pereira, Luiz Henrique GarciaCastro, Jerson Rogelio ChanchayVargas, Pilar Mireya HuatatocaGomez, Jose Alejandro MoralesOliveira, Claudio [UNESP]2021-06-25T10:29:01Z2021-06-25T10:29:01Z2021-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article103-116http://dx.doi.org/10.1007/s10709-021-00118-6Genetica, v. 149, n. 2, p. 103-116, 2021.1573-68570016-6707http://hdl.handle.net/11449/20624910.1007/s10709-021-00118-62-s2.0-85104904198Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGeneticainfo:eu-repo/semantics/openAccess2021-10-23T01:58:00Zoai:repositorio.unesp.br:11449/206249Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:19:24.608253Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The use of an integrative approach to improve accuracy of species identification and detection of new species in studies of stream fish diversity |
title |
The use of an integrative approach to improve accuracy of species identification and detection of new species in studies of stream fish diversity |
spellingShingle |
The use of an integrative approach to improve accuracy of species identification and detection of new species in studies of stream fish diversity Pereira, Luiz Henrique Garcia Checklist Conservation DNA barcoding Fish distribution Fishes Paraná River basin |
title_short |
The use of an integrative approach to improve accuracy of species identification and detection of new species in studies of stream fish diversity |
title_full |
The use of an integrative approach to improve accuracy of species identification and detection of new species in studies of stream fish diversity |
title_fullStr |
The use of an integrative approach to improve accuracy of species identification and detection of new species in studies of stream fish diversity |
title_full_unstemmed |
The use of an integrative approach to improve accuracy of species identification and detection of new species in studies of stream fish diversity |
title_sort |
The use of an integrative approach to improve accuracy of species identification and detection of new species in studies of stream fish diversity |
author |
Pereira, Luiz Henrique Garcia |
author_facet |
Pereira, Luiz Henrique Garcia Castro, Jerson Rogelio Chanchay Vargas, Pilar Mireya Huatatoca Gomez, Jose Alejandro Morales Oliveira, Claudio [UNESP] |
author_role |
author |
author2 |
Castro, Jerson Rogelio Chanchay Vargas, Pilar Mireya Huatatoca Gomez, Jose Alejandro Morales Oliveira, Claudio [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal da Integração Latino-Americana – UNILA Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pereira, Luiz Henrique Garcia Castro, Jerson Rogelio Chanchay Vargas, Pilar Mireya Huatatoca Gomez, Jose Alejandro Morales Oliveira, Claudio [UNESP] |
dc.subject.por.fl_str_mv |
Checklist Conservation DNA barcoding Fish distribution Fishes Paraná River basin |
topic |
Checklist Conservation DNA barcoding Fish distribution Fishes Paraná River basin |
description |
In this study, we made an inventory of the stream and headwater ichthyofauna of the left bank of the Itaipu Dam Reservoir, located in the lower part of the Upper Paraná River basin, using an integrative approach of molecular and morphological data. The area is located in the western portion of the Paraná state in Brazil, in an area of about 8,000 km2 highly impacted by deforestation and intensive agriculture. For taxonomic identification of species, we used an identification key combined with the DNA barcoding approach. We found 48 species belonging to six orders, 13 families, and 37 genera. The Siluriformes and Characiformes were the most representative orders (75%) and the Characidae was the most representative family (20.8%). Nine species prevailed in this region, making up to 86% of all specimens collected. The integrative approach proved to be useful by allowing the unambiguous identification of all species, including those cases in which morphological characters were not conclusive for species identification, cases of cryptic species, and species with high morphological plasticity. In addition, the integrative approach highlighted six to 13 new putative species depending on the approach considered. Our study provides a relevant contribution to the knowledge of fish diversity in a poorly studied area of the Paraná River basin. We showed that the use of an integrative approach in inventory studies improves species identification and the discovery of new, cryptic, and overlooked species, being a powerful and necessary tool to quantify biodiversity. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:29:01Z 2021-06-25T10:29:01Z 2021-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10709-021-00118-6 Genetica, v. 149, n. 2, p. 103-116, 2021. 1573-6857 0016-6707 http://hdl.handle.net/11449/206249 10.1007/s10709-021-00118-6 2-s2.0-85104904198 |
url |
http://dx.doi.org/10.1007/s10709-021-00118-6 http://hdl.handle.net/11449/206249 |
identifier_str_mv |
Genetica, v. 149, n. 2, p. 103-116, 2021. 1573-6857 0016-6707 10.1007/s10709-021-00118-6 2-s2.0-85104904198 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
103-116 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128919452254208 |