Behavioral, Ventilatory and Thermoregulatory Responses to Hypercapnia and Hypoxia in the Wistar Audiogenic Rat (WAR) Strain
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0154141 http://hdl.handle.net/11449/161489 |
Resumo: | Introduction We investigated the behavioral, respiratory, and thermoregulatory responses elicited by acute exposure to both hypercapnic and hypoxic environments in Wistar audiogenic rats (WARs). The WAR strain represents a genetic animal model of epilepsy. Methods Behavioral analyses were performed using neuroethological methods, and flowcharts were constructed to illustrate behavioral findings. The body plethysmography method was used to obtain pulmonary ventilation (VE) measurements, and body temperature (Tb) measurements were taken via temperature sensors implanted in the abdominal cavities of the animals. Results No significant difference was observed between the WAR and Wistar control group with respect to the thermoregulatory response elicited by exposure to both acute hypercapnia and acute hypoxia (p>0.05). However, we found that the VE of WARs was attenuated relative to that of Wistar control animals during exposure to both hypercapnic (WAR: 133 +/- 11% vs. Wistar: 243 +/- 23%, p<0.01) and hypoxic conditions (WAR: 138 +/- 8% vs. Wistar: 177 +/- 8%; p<0.01). In addition, we noted that this ventilatory attenuation was followed by alterations in the behavioral responses of these animals. Conclusions Our results indicate that WARs, a genetic model of epilepsy, have important alterations in their ability to compensate for changes in levels of various arterial blood gasses. WARs present an attenuated ventilatory response to an increased PaCO2 or decreased PaO2, coupled to behavioral changes, which make them a suitable model to further study respiratory risks associated to epilepsy. |
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Behavioral, Ventilatory and Thermoregulatory Responses to Hypercapnia and Hypoxia in the Wistar Audiogenic Rat (WAR) StrainIntroduction We investigated the behavioral, respiratory, and thermoregulatory responses elicited by acute exposure to both hypercapnic and hypoxic environments in Wistar audiogenic rats (WARs). The WAR strain represents a genetic animal model of epilepsy. Methods Behavioral analyses were performed using neuroethological methods, and flowcharts were constructed to illustrate behavioral findings. The body plethysmography method was used to obtain pulmonary ventilation (VE) measurements, and body temperature (Tb) measurements were taken via temperature sensors implanted in the abdominal cavities of the animals. Results No significant difference was observed between the WAR and Wistar control group with respect to the thermoregulatory response elicited by exposure to both acute hypercapnia and acute hypoxia (p>0.05). However, we found that the VE of WARs was attenuated relative to that of Wistar control animals during exposure to both hypercapnic (WAR: 133 +/- 11% vs. Wistar: 243 +/- 23%, p<0.01) and hypoxic conditions (WAR: 138 +/- 8% vs. Wistar: 177 +/- 8%; p<0.01). In addition, we noted that this ventilatory attenuation was followed by alterations in the behavioral responses of these animals. Conclusions Our results indicate that WARs, a genetic model of epilepsy, have important alterations in their ability to compensate for changes in levels of various arterial blood gasses. WARs present an attenuated ventilatory response to an increased PaCO2 or decreased PaO2, coupled to behavioral changes, which make them a suitable model to further study respiratory risks associated to epilepsy.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Physiol, BR-14049900 Ribeirao Preto, SP, BrazilUniv Estadual Feira de Santana UEFS, Dept Ciencias Biol DCBIO, Av Transnordestina S-N, BR-44036900 Feira De Santana, BA, BrazilSao Paulo State Univ UNESP FCAV, Dept Morphol & Anim Physiol, Via Acesso Prof Paulo D Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilSao Paulo State Univ UNESP FCAV, Dept Morphol & Anim Physiol, Via Acesso Prof Paulo D Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 06/60768-6FAPESP: 05/56447-7FAPESP: 2007/50261-4FAPESP: FAPESP-Cinapce 2005/56447-7CAPES: 02417/09-0FAPESP: 2013/17606-9Public Library ScienceUniversidade de São Paulo (USP)Univ Estadual Feira de Santana UEFSUniversidade Estadual Paulista (Unesp)Granjeiro, Erica MariaSilva, Glauber S. F. da [UNESP]Giusti, HumbertoOliveira, Jose AntonioGlass, Mogens LesnerGarcia-Cairasco, Norberto2018-11-26T16:32:56Z2018-11-26T16:32:56Z2016-05-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16application/pdfhttp://dx.doi.org/10.1371/journal.pone.0154141Plos One. San Francisco: Public Library Science, v. 11, n. 5, 16 p., 2016.1932-6203http://hdl.handle.net/11449/16148910.1371/journal.pone.0154141WOS:000375676800021WOS000375676800021.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One1,164info:eu-repo/semantics/openAccess2024-06-06T18:41:56Zoai:repositorio.unesp.br:11449/161489Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:27:39.325003Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Behavioral, Ventilatory and Thermoregulatory Responses to Hypercapnia and Hypoxia in the Wistar Audiogenic Rat (WAR) Strain |
title |
Behavioral, Ventilatory and Thermoregulatory Responses to Hypercapnia and Hypoxia in the Wistar Audiogenic Rat (WAR) Strain |
spellingShingle |
Behavioral, Ventilatory and Thermoregulatory Responses to Hypercapnia and Hypoxia in the Wistar Audiogenic Rat (WAR) Strain Granjeiro, Erica Maria |
title_short |
Behavioral, Ventilatory and Thermoregulatory Responses to Hypercapnia and Hypoxia in the Wistar Audiogenic Rat (WAR) Strain |
title_full |
Behavioral, Ventilatory and Thermoregulatory Responses to Hypercapnia and Hypoxia in the Wistar Audiogenic Rat (WAR) Strain |
title_fullStr |
Behavioral, Ventilatory and Thermoregulatory Responses to Hypercapnia and Hypoxia in the Wistar Audiogenic Rat (WAR) Strain |
title_full_unstemmed |
Behavioral, Ventilatory and Thermoregulatory Responses to Hypercapnia and Hypoxia in the Wistar Audiogenic Rat (WAR) Strain |
title_sort |
Behavioral, Ventilatory and Thermoregulatory Responses to Hypercapnia and Hypoxia in the Wistar Audiogenic Rat (WAR) Strain |
author |
Granjeiro, Erica Maria |
author_facet |
Granjeiro, Erica Maria Silva, Glauber S. F. da [UNESP] Giusti, Humberto Oliveira, Jose Antonio Glass, Mogens Lesner Garcia-Cairasco, Norberto |
author_role |
author |
author2 |
Silva, Glauber S. F. da [UNESP] Giusti, Humberto Oliveira, Jose Antonio Glass, Mogens Lesner Garcia-Cairasco, Norberto |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Univ Estadual Feira de Santana UEFS Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Granjeiro, Erica Maria Silva, Glauber S. F. da [UNESP] Giusti, Humberto Oliveira, Jose Antonio Glass, Mogens Lesner Garcia-Cairasco, Norberto |
description |
Introduction We investigated the behavioral, respiratory, and thermoregulatory responses elicited by acute exposure to both hypercapnic and hypoxic environments in Wistar audiogenic rats (WARs). The WAR strain represents a genetic animal model of epilepsy. Methods Behavioral analyses were performed using neuroethological methods, and flowcharts were constructed to illustrate behavioral findings. The body plethysmography method was used to obtain pulmonary ventilation (VE) measurements, and body temperature (Tb) measurements were taken via temperature sensors implanted in the abdominal cavities of the animals. Results No significant difference was observed between the WAR and Wistar control group with respect to the thermoregulatory response elicited by exposure to both acute hypercapnia and acute hypoxia (p>0.05). However, we found that the VE of WARs was attenuated relative to that of Wistar control animals during exposure to both hypercapnic (WAR: 133 +/- 11% vs. Wistar: 243 +/- 23%, p<0.01) and hypoxic conditions (WAR: 138 +/- 8% vs. Wistar: 177 +/- 8%; p<0.01). In addition, we noted that this ventilatory attenuation was followed by alterations in the behavioral responses of these animals. Conclusions Our results indicate that WARs, a genetic model of epilepsy, have important alterations in their ability to compensate for changes in levels of various arterial blood gasses. WARs present an attenuated ventilatory response to an increased PaCO2 or decreased PaO2, coupled to behavioral changes, which make them a suitable model to further study respiratory risks associated to epilepsy. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-05-05 2018-11-26T16:32:56Z 2018-11-26T16:32:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0154141 Plos One. San Francisco: Public Library Science, v. 11, n. 5, 16 p., 2016. 1932-6203 http://hdl.handle.net/11449/161489 10.1371/journal.pone.0154141 WOS:000375676800021 WOS000375676800021.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0154141 http://hdl.handle.net/11449/161489 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 11, n. 5, 16 p., 2016. 1932-6203 10.1371/journal.pone.0154141 WOS:000375676800021 WOS000375676800021.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One 1,164 |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
16 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
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Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1808129204816969728 |