Growth hormone mRNA expression in the pituitary of Bos indicus and Bos taurus x Bos indicus crossbred young bulls treated with recombinant bovine somatotropin
Autor(a) principal: | |
---|---|
Data de Publicação: | 2002 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.geneticsmr.com//year2002/vol1-4/pdf/gmr0036.pdf http://hdl.handle.net/11449/67113 |
Resumo: | The effects of breed and of recombinant bovine somatotropin (rbST) treatment on growth hormone gene expression were studied in young bulls. The experiment was completely randomized in a [2 × 2]-factorial arrangement, using two levels of rbst (0 or 250 mg/animal/14 days), and two breed groups (Nelore and Simmental x Nelore crossbred). A CDNA encoding Bos indicus growth hormone was cloned and sequenced for use as a probe in Northern and dot blot analyses. Compared to the Bos taurus structural gene, the Bos indicus CDNA was found to begin 21 bases downstream from the transcription initiation site and had only two discrepancies (C to T at position 144-His and T to C at position 354-Phe), without changes in the polypeptide sequence. However, two amino acid substitutions were found for Bubalus spp., which belong to the same tribe. The rbst treatment did not change any of the characteristics evaluated (body and pituitary gland weights, growth hormone MRNA expression level). Crossbred animals had significantly higher body weight and heavier pituitaries than Nelore cattle. Pituitary weight was proportional to body weight in both breed groups. Growth hormone MRNA expression in the pituitary was similar (P>0.075) for both breed and hormonal treatment groups, but was 31.9% higher in the pure Nelore group, suggesting that growth hormone gene transcription regulation differs among these breeds. |
id |
UNSP_13be10a4b8309bd563abc9ee09f903b4 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/67113 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Growth hormone mRNA expression in the pituitary of Bos indicus and Bos taurus x Bos indicus crossbred young bulls treated with recombinant bovine somatotropinBos indicusBos taurusBovine pituitary Growth hormone gene expressionRecombinant bovine somatotropincomplementary DNAgrowth hormonegrowth hormone, bovinemessenger RNAamino acid substitutionanimal experimentanimal tissuebody weightcattlecomparative studycontrolled studycross breedingdot hybridizationgene expressiongene sequencehypophysismalemolecular cloningnonhumanNorthern blottingquantitative analysisanimalDNA sequencedrug effectgeneticsgrowth, development and agingorgan sizesecretionAnimaliaBosBovinaeBubalusAnimalsBody WeightCattleDNA, ComplementaryGene ExpressionGrowth HormoneMaleOrgan SizePituitary GlandRNA, MessengerSequence Analysis, DNAThe effects of breed and of recombinant bovine somatotropin (rbST) treatment on growth hormone gene expression were studied in young bulls. The experiment was completely randomized in a [2 × 2]-factorial arrangement, using two levels of rbst (0 or 250 mg/animal/14 days), and two breed groups (Nelore and Simmental x Nelore crossbred). A CDNA encoding Bos indicus growth hormone was cloned and sequenced for use as a probe in Northern and dot blot analyses. Compared to the Bos taurus structural gene, the Bos indicus CDNA was found to begin 21 bases downstream from the transcription initiation site and had only two discrepancies (C to T at position 144-His and T to C at position 354-Phe), without changes in the polypeptide sequence. However, two amino acid substitutions were found for Bubalus spp., which belong to the same tribe. The rbst treatment did not change any of the characteristics evaluated (body and pituitary gland weights, growth hormone MRNA expression level). Crossbred animals had significantly higher body weight and heavier pituitaries than Nelore cattle. Pituitary weight was proportional to body weight in both breed groups. Growth hormone MRNA expression in the pituitary was similar (P>0.075) for both breed and hormonal treatment groups, but was 31.9% higher in the pure Nelore group, suggesting that growth hormone gene transcription regulation differs among these breeds.Departamento de Tecnologia Fac. de Cie. Agrarias e Veterinarias UNESP, Rodovia Carlos Tonanni, km 5, 14884-900 Jaboticabal, SPDepto. de Morfol. e Fisiol. Animal Fac. de Cie. Agrarias e Veterinarias UNESP, Rodovia Carlos Tonanni, km 5, 14884-900 Jaboticabal, SPDepto. de Melhoramento e Nutr. Anim. Faculdade de Med. Vet. e Zootecnia UNESP, Caixa Postal 560, 18600-000 Botucatu, SPDepartamento de Tecnologia Fac. de Cie. Agrarias e Veterinarias UNESP, Rodovia Carlos Tonanni, km 5, 14884-900 Jaboticabal, SPDepto. de Morfol. e Fisiol. Animal Fac. de Cie. Agrarias e Veterinarias UNESP, Rodovia Carlos Tonanni, km 5, 14884-900 Jaboticabal, SPDepto. de Melhoramento e Nutr. Anim. Faculdade de Med. Vet. e Zootecnia UNESP, Caixa Postal 560, 18600-000 Botucatu, SPUniversidade Estadual Paulista (Unesp)Di Mauro, S. M Z [UNESP]Furlan, L. R. [UNESP]Ferro, M. I T [UNESP]Macari, Marcos [UNESP]Ferro, J. A. [UNESP]2014-05-27T11:20:34Z2014-05-27T11:20:34Z2002-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article327-336application/pdfhttp://www.geneticsmr.com//year2002/vol1-4/pdf/gmr0036.pdfGenetics and Molecular Research, v. 1, n. 4, p. 327-336, 2002.1676-5680http://hdl.handle.net/11449/671132-s2.0-24425748172-s2.0-2442574817.pdf57135585729266690147241723612464Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2024-09-06T18:55:51Zoai:repositorio.unesp.br:11449/67113Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-06T18:55:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Growth hormone mRNA expression in the pituitary of Bos indicus and Bos taurus x Bos indicus crossbred young bulls treated with recombinant bovine somatotropin |
title |
Growth hormone mRNA expression in the pituitary of Bos indicus and Bos taurus x Bos indicus crossbred young bulls treated with recombinant bovine somatotropin |
spellingShingle |
Growth hormone mRNA expression in the pituitary of Bos indicus and Bos taurus x Bos indicus crossbred young bulls treated with recombinant bovine somatotropin Di Mauro, S. M Z [UNESP] Bos indicus Bos taurus Bovine pituitary Growth hormone gene expression Recombinant bovine somatotropin complementary DNA growth hormone growth hormone, bovine messenger RNA amino acid substitution animal experiment animal tissue body weight cattle comparative study controlled study cross breeding dot hybridization gene expression gene sequence hypophysis male molecular cloning nonhuman Northern blotting quantitative analysis animal DNA sequence drug effect genetics growth, development and aging organ size secretion Animalia Bos Bovinae Bubalus Animals Body Weight Cattle DNA, Complementary Gene Expression Growth Hormone Male Organ Size Pituitary Gland RNA, Messenger Sequence Analysis, DNA |
title_short |
Growth hormone mRNA expression in the pituitary of Bos indicus and Bos taurus x Bos indicus crossbred young bulls treated with recombinant bovine somatotropin |
title_full |
Growth hormone mRNA expression in the pituitary of Bos indicus and Bos taurus x Bos indicus crossbred young bulls treated with recombinant bovine somatotropin |
title_fullStr |
Growth hormone mRNA expression in the pituitary of Bos indicus and Bos taurus x Bos indicus crossbred young bulls treated with recombinant bovine somatotropin |
title_full_unstemmed |
Growth hormone mRNA expression in the pituitary of Bos indicus and Bos taurus x Bos indicus crossbred young bulls treated with recombinant bovine somatotropin |
title_sort |
Growth hormone mRNA expression in the pituitary of Bos indicus and Bos taurus x Bos indicus crossbred young bulls treated with recombinant bovine somatotropin |
author |
Di Mauro, S. M Z [UNESP] |
author_facet |
Di Mauro, S. M Z [UNESP] Furlan, L. R. [UNESP] Ferro, M. I T [UNESP] Macari, Marcos [UNESP] Ferro, J. A. [UNESP] |
author_role |
author |
author2 |
Furlan, L. R. [UNESP] Ferro, M. I T [UNESP] Macari, Marcos [UNESP] Ferro, J. A. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Di Mauro, S. M Z [UNESP] Furlan, L. R. [UNESP] Ferro, M. I T [UNESP] Macari, Marcos [UNESP] Ferro, J. A. [UNESP] |
dc.subject.por.fl_str_mv |
Bos indicus Bos taurus Bovine pituitary Growth hormone gene expression Recombinant bovine somatotropin complementary DNA growth hormone growth hormone, bovine messenger RNA amino acid substitution animal experiment animal tissue body weight cattle comparative study controlled study cross breeding dot hybridization gene expression gene sequence hypophysis male molecular cloning nonhuman Northern blotting quantitative analysis animal DNA sequence drug effect genetics growth, development and aging organ size secretion Animalia Bos Bovinae Bubalus Animals Body Weight Cattle DNA, Complementary Gene Expression Growth Hormone Male Organ Size Pituitary Gland RNA, Messenger Sequence Analysis, DNA |
topic |
Bos indicus Bos taurus Bovine pituitary Growth hormone gene expression Recombinant bovine somatotropin complementary DNA growth hormone growth hormone, bovine messenger RNA amino acid substitution animal experiment animal tissue body weight cattle comparative study controlled study cross breeding dot hybridization gene expression gene sequence hypophysis male molecular cloning nonhuman Northern blotting quantitative analysis animal DNA sequence drug effect genetics growth, development and aging organ size secretion Animalia Bos Bovinae Bubalus Animals Body Weight Cattle DNA, Complementary Gene Expression Growth Hormone Male Organ Size Pituitary Gland RNA, Messenger Sequence Analysis, DNA |
description |
The effects of breed and of recombinant bovine somatotropin (rbST) treatment on growth hormone gene expression were studied in young bulls. The experiment was completely randomized in a [2 × 2]-factorial arrangement, using two levels of rbst (0 or 250 mg/animal/14 days), and two breed groups (Nelore and Simmental x Nelore crossbred). A CDNA encoding Bos indicus growth hormone was cloned and sequenced for use as a probe in Northern and dot blot analyses. Compared to the Bos taurus structural gene, the Bos indicus CDNA was found to begin 21 bases downstream from the transcription initiation site and had only two discrepancies (C to T at position 144-His and T to C at position 354-Phe), without changes in the polypeptide sequence. However, two amino acid substitutions were found for Bubalus spp., which belong to the same tribe. The rbst treatment did not change any of the characteristics evaluated (body and pituitary gland weights, growth hormone MRNA expression level). Crossbred animals had significantly higher body weight and heavier pituitaries than Nelore cattle. Pituitary weight was proportional to body weight in both breed groups. Growth hormone MRNA expression in the pituitary was similar (P>0.075) for both breed and hormonal treatment groups, but was 31.9% higher in the pure Nelore group, suggesting that growth hormone gene transcription regulation differs among these breeds. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-12-01 2014-05-27T11:20:34Z 2014-05-27T11:20:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.geneticsmr.com//year2002/vol1-4/pdf/gmr0036.pdf Genetics and Molecular Research, v. 1, n. 4, p. 327-336, 2002. 1676-5680 http://hdl.handle.net/11449/67113 2-s2.0-2442574817 2-s2.0-2442574817.pdf 5713558572926669 0147241723612464 |
url |
http://www.geneticsmr.com//year2002/vol1-4/pdf/gmr0036.pdf http://hdl.handle.net/11449/67113 |
identifier_str_mv |
Genetics and Molecular Research, v. 1, n. 4, p. 327-336, 2002. 1676-5680 2-s2.0-2442574817 2-s2.0-2442574817.pdf 5713558572926669 0147241723612464 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Research 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
327-336 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021350209748992 |