Fractal dimension analysis reveals skeletal muscle disorganization in mdx mice
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bbrc.2018.05.189 http://hdl.handle.net/11449/179911 |
Resumo: | Duchenne Muscular Dystrophy (DMD) is characterized by muscle extracellular matrix disorganization due to the increased collagen deposition leading to fibrosis that significantly exacerbates disease progression. Fractal dimension analysis is a method that quantifies tissue/cellular disorganization and characterizes complex structures. The first objective of the present study was use fractal analysis to evaluate extracellular matrix disorganization in mdx mice soleus muscle. Next, we mimic a hyper-proliferation of fibrogenic cells by co-culturing NIH3T3 fibroblasts and C2C12 myoblasts to test whether fibroblasts induce disorganization in myoblast arrangement. Here, we show mdx presented high skeletal muscle disorganization as revealed by fractal analysis. Similarly, this method revealed that myoblasts co-cultured with fibroblast also presented cellular arrangement disorganization. We also reanalyzed skeletal muscle microarrays transcriptomic data from mdx and DMD patients that revealed transcripts related to extracellular matrix organization. This analysis also identified Osteoglycin, which was validated as a potential regulator of ECM organization in mdx dystrophic muscles. Our results demonstrate that fractal dimension is useful tool for the analysis of skeletal muscle disorganization in DMD and also reveal a fibroblast-myoblast cross-talk that contributes to “in vitro” myoblast disarrangement. |
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Fractal dimension analysis reveals skeletal muscle disorganization in mdx miceCo-cultureDuchenne muscular dystrophyFractal dimension analysisHistopathologyOsteoglycinDuchenne Muscular Dystrophy (DMD) is characterized by muscle extracellular matrix disorganization due to the increased collagen deposition leading to fibrosis that significantly exacerbates disease progression. Fractal dimension analysis is a method that quantifies tissue/cellular disorganization and characterizes complex structures. The first objective of the present study was use fractal analysis to evaluate extracellular matrix disorganization in mdx mice soleus muscle. Next, we mimic a hyper-proliferation of fibrogenic cells by co-culturing NIH3T3 fibroblasts and C2C12 myoblasts to test whether fibroblasts induce disorganization in myoblast arrangement. Here, we show mdx presented high skeletal muscle disorganization as revealed by fractal analysis. Similarly, this method revealed that myoblasts co-cultured with fibroblast also presented cellular arrangement disorganization. We also reanalyzed skeletal muscle microarrays transcriptomic data from mdx and DMD patients that revealed transcripts related to extracellular matrix organization. This analysis also identified Osteoglycin, which was validated as a potential regulator of ECM organization in mdx dystrophic muscles. Our results demonstrate that fractal dimension is useful tool for the analysis of skeletal muscle disorganization in DMD and also reveal a fibroblast-myoblast cross-talk that contributes to “in vitro” myoblast disarrangement.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Morphology Institute of Biosciences São Paulo State University (UNESP)Department of Anatomy Institute of Biosciences São Paulo State University (UNESP)Department of Physiotherapy University of Western São Paulo (UNOESTE)Department of Morphology Institute of Biosciences São Paulo State University (UNESP)Department of Anatomy Institute of Biosciences São Paulo State University (UNESP)FAPESP: 2012/13961-6CNPq: 476399/2013-0CNPq: 800571/2016-9Universidade Estadual Paulista (Unesp)University of Western São Paulo (UNOESTE)Cury, Sarah Santiloni [UNESP]Freire, Paula Paccielli [UNESP]Martinucci, Bruno [UNESP]dos Santos, Veridiana Carvalho [UNESP]de Oliveira, Grasieli [UNESP]Ferretti, Renato [UNESP]Dal-Pai-Silva, Maeli [UNESP]Pacagnelli, Francis LopesDelella, Flávia Karina [UNESP]Carvalho, Robson Francisco [UNESP]2018-12-11T17:37:16Z2018-12-11T17:37:16Z2018-09-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article109-115application/pdfhttp://dx.doi.org/10.1016/j.bbrc.2018.05.189Biochemical and Biophysical Research Communications, v. 503, n. 1, p. 109-115, 2018.1090-21040006-291Xhttp://hdl.handle.net/11449/17991110.1016/j.bbrc.2018.05.1892-s2.0-850478926472-s2.0-85047892647.pdf36816131600861750000-0003-3944-1906Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiochemical and Biophysical Research Communications1,087info:eu-repo/semantics/openAccess2024-01-21T06:26:09Zoai:repositorio.unesp.br:11449/179911Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:36:40.795549Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fractal dimension analysis reveals skeletal muscle disorganization in mdx mice |
title |
Fractal dimension analysis reveals skeletal muscle disorganization in mdx mice |
spellingShingle |
Fractal dimension analysis reveals skeletal muscle disorganization in mdx mice Cury, Sarah Santiloni [UNESP] Co-culture Duchenne muscular dystrophy Fractal dimension analysis Histopathology Osteoglycin |
title_short |
Fractal dimension analysis reveals skeletal muscle disorganization in mdx mice |
title_full |
Fractal dimension analysis reveals skeletal muscle disorganization in mdx mice |
title_fullStr |
Fractal dimension analysis reveals skeletal muscle disorganization in mdx mice |
title_full_unstemmed |
Fractal dimension analysis reveals skeletal muscle disorganization in mdx mice |
title_sort |
Fractal dimension analysis reveals skeletal muscle disorganization in mdx mice |
author |
Cury, Sarah Santiloni [UNESP] |
author_facet |
Cury, Sarah Santiloni [UNESP] Freire, Paula Paccielli [UNESP] Martinucci, Bruno [UNESP] dos Santos, Veridiana Carvalho [UNESP] de Oliveira, Grasieli [UNESP] Ferretti, Renato [UNESP] Dal-Pai-Silva, Maeli [UNESP] Pacagnelli, Francis Lopes Delella, Flávia Karina [UNESP] Carvalho, Robson Francisco [UNESP] |
author_role |
author |
author2 |
Freire, Paula Paccielli [UNESP] Martinucci, Bruno [UNESP] dos Santos, Veridiana Carvalho [UNESP] de Oliveira, Grasieli [UNESP] Ferretti, Renato [UNESP] Dal-Pai-Silva, Maeli [UNESP] Pacagnelli, Francis Lopes Delella, Flávia Karina [UNESP] Carvalho, Robson Francisco [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Western São Paulo (UNOESTE) |
dc.contributor.author.fl_str_mv |
Cury, Sarah Santiloni [UNESP] Freire, Paula Paccielli [UNESP] Martinucci, Bruno [UNESP] dos Santos, Veridiana Carvalho [UNESP] de Oliveira, Grasieli [UNESP] Ferretti, Renato [UNESP] Dal-Pai-Silva, Maeli [UNESP] Pacagnelli, Francis Lopes Delella, Flávia Karina [UNESP] Carvalho, Robson Francisco [UNESP] |
dc.subject.por.fl_str_mv |
Co-culture Duchenne muscular dystrophy Fractal dimension analysis Histopathology Osteoglycin |
topic |
Co-culture Duchenne muscular dystrophy Fractal dimension analysis Histopathology Osteoglycin |
description |
Duchenne Muscular Dystrophy (DMD) is characterized by muscle extracellular matrix disorganization due to the increased collagen deposition leading to fibrosis that significantly exacerbates disease progression. Fractal dimension analysis is a method that quantifies tissue/cellular disorganization and characterizes complex structures. The first objective of the present study was use fractal analysis to evaluate extracellular matrix disorganization in mdx mice soleus muscle. Next, we mimic a hyper-proliferation of fibrogenic cells by co-culturing NIH3T3 fibroblasts and C2C12 myoblasts to test whether fibroblasts induce disorganization in myoblast arrangement. Here, we show mdx presented high skeletal muscle disorganization as revealed by fractal analysis. Similarly, this method revealed that myoblasts co-cultured with fibroblast also presented cellular arrangement disorganization. We also reanalyzed skeletal muscle microarrays transcriptomic data from mdx and DMD patients that revealed transcripts related to extracellular matrix organization. This analysis also identified Osteoglycin, which was validated as a potential regulator of ECM organization in mdx dystrophic muscles. Our results demonstrate that fractal dimension is useful tool for the analysis of skeletal muscle disorganization in DMD and also reveal a fibroblast-myoblast cross-talk that contributes to “in vitro” myoblast disarrangement. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:37:16Z 2018-12-11T17:37:16Z 2018-09-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bbrc.2018.05.189 Biochemical and Biophysical Research Communications, v. 503, n. 1, p. 109-115, 2018. 1090-2104 0006-291X http://hdl.handle.net/11449/179911 10.1016/j.bbrc.2018.05.189 2-s2.0-85047892647 2-s2.0-85047892647.pdf 3681613160086175 0000-0003-3944-1906 |
url |
http://dx.doi.org/10.1016/j.bbrc.2018.05.189 http://hdl.handle.net/11449/179911 |
identifier_str_mv |
Biochemical and Biophysical Research Communications, v. 503, n. 1, p. 109-115, 2018. 1090-2104 0006-291X 10.1016/j.bbrc.2018.05.189 2-s2.0-85047892647 2-s2.0-85047892647.pdf 3681613160086175 0000-0003-3944-1906 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochemical and Biophysical Research Communications 1,087 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
109-115 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129536235143168 |