Proteinogram and serum IgG concentration in newborn foals up to thirty days of life treated with plasma
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-5150-PVB-5388 http://hdl.handle.net/11449/164468 |
Resumo: | The aim or this study was to evaluate serum protein ana serum IgG concentrations [after a direct enzyme immunoassay test ELISA optimization) in newborns foals from birth to thirty days of life before and after colostrum consumption and intravenous treatment with plasma. Twenty foals and their respective progenitors as well as four plasma donor's horses were used. Blood samples were obtained from newborn foals at five time points, immediately after birth and before colostrum intake (Ml), ten hours after birth (M2), 24 hours after birth and prior administration of blood plasma (M3), 48 hours after birth and 24 hours after plasma administration (M4), and 30 days after birth (M5). Blood and colostrum samples were collected from the progenitor mares immediately postpartum. Concentration of total protein (TP) and albumin were determined using a biochemical analyzer. The TP concentration was also measured by refractometer. Fractions of total serum protein were separated using agarose gel electrophoresis. Colostrum density was evaluated using BRIX refractometer and specific density colostrometer. Total IgG concentration was determined by an enzyme-linked immunosorbent assay. With the ELISA system proposed here it was possible to determine IgG concentrations in serum, plasma, and equine colostrum samples with adequate repeatability. Serum IgG concentration in foals at birth was 15 +/- 8mg/dL (mean +/- standard deviation) raising at ten hours (2,408 +/- 608mg/dL) and remaining at similar levels up to 48 hours of life (2,364 +/- 784m g/dL), and decreasing significantly at 30 days of age (1,414 +/- 586mg/dL). Serum and colostrum IgG concentrations of mares were 1,746 +/- 505mg/dL and 7,714 +/- 2,619mg/dL, respectively. The plasma IgG concentrations from donor mares were 2,026 +/- 148mg/dL. Total protein, total globulins, and gamma-globulin fraction showed correlation with IgG. Ten hours post birth was an adequate time to verify the transfer of passive immunity, allowing to adoption prophylactic and/or therapeutic measures in a horse farms. One liter of plasma administered at 24 hours of life was not sufficient to raise serum IgG concentrations in foals without passive immunity transfer failure. |
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Proteinogram and serum IgG concentration in newborn foals up to thirty days of life treated with plasmaProteinogramIgGfoalsplasmaequineenzyme immunoassayelectrophoresisimmunoglobulinsproteinsThe aim or this study was to evaluate serum protein ana serum IgG concentrations [after a direct enzyme immunoassay test ELISA optimization) in newborns foals from birth to thirty days of life before and after colostrum consumption and intravenous treatment with plasma. Twenty foals and their respective progenitors as well as four plasma donor's horses were used. Blood samples were obtained from newborn foals at five time points, immediately after birth and before colostrum intake (Ml), ten hours after birth (M2), 24 hours after birth and prior administration of blood plasma (M3), 48 hours after birth and 24 hours after plasma administration (M4), and 30 days after birth (M5). Blood and colostrum samples were collected from the progenitor mares immediately postpartum. Concentration of total protein (TP) and albumin were determined using a biochemical analyzer. The TP concentration was also measured by refractometer. Fractions of total serum protein were separated using agarose gel electrophoresis. Colostrum density was evaluated using BRIX refractometer and specific density colostrometer. Total IgG concentration was determined by an enzyme-linked immunosorbent assay. With the ELISA system proposed here it was possible to determine IgG concentrations in serum, plasma, and equine colostrum samples with adequate repeatability. Serum IgG concentration in foals at birth was 15 +/- 8mg/dL (mean +/- standard deviation) raising at ten hours (2,408 +/- 608mg/dL) and remaining at similar levels up to 48 hours of life (2,364 +/- 784m g/dL), and decreasing significantly at 30 days of age (1,414 +/- 586mg/dL). Serum and colostrum IgG concentrations of mares were 1,746 +/- 505mg/dL and 7,714 +/- 2,619mg/dL, respectively. The plasma IgG concentrations from donor mares were 2,026 +/- 148mg/dL. Total protein, total globulins, and gamma-globulin fraction showed correlation with IgG. Ten hours post birth was an adequate time to verify the transfer of passive immunity, allowing to adoption prophylactic and/or therapeutic measures in a horse farms. One liter of plasma administered at 24 hours of life was not sufficient to raise serum IgG concentrations in foals without passive immunity transfer failure.Univ Estadual Paulista Unesp, Fac Med Vet & Zootecnia, Dept Ciln Vet, Campus Botucatu, BR-18618970 Botucatu, SP, BrazilUniv Fed Uberlandia, Fac Med Vet FAMEV, BR-38405314 Uberlandia, MG, BrazilMississippi State Univ, Coll Vet Med, Dept Pathobiol & Populat Med, Mississippi State, MS 39762 USAUniv Estadual Paulista Unesp, Inst Biociencia Botucatu, Dept Microbiol & Imunol, BR-18618691 Botucatu, SP, BrazilUniv Estadual Paulista Unesp, Fac Med Vet & Zootecnia, Dept Ciln Vet, Campus Botucatu, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista Unesp, Inst Biociencia Botucatu, Dept Microbiol & Imunol, BR-18618691 Botucatu, SP, BrazilRevista Pesquisa Veterinaria BrasileiraUniversidade Estadual Paulista (Unesp)Universidade Federal de Uberlândia (UFU)Mississippi State UnivNeto, Campo A. V. C. [UNESP]Oliveira-Filho, Jose P. [UNESP]Delfiol, Diego J. Z.Badial, Peres R.Araujo Junior, Joao P. [UNESP]Cruz, Tais E. [UNESP]Tenorio, Michely S. [UNESP]Borges, Alexandre S. [UNESP]2018-11-26T17:54:40Z2018-11-26T17:54:40Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article795-805application/pdfhttp://dx.doi.org/10.1590/1678-5150-PVB-5388Pesquisa Veterinaria Brasileira. Rio Janeiro: Revista Pesquisa Veterinaria Brasileira, v. 38, n. 5, p. 795-805, 2018.0100-736Xhttp://hdl.handle.net/11449/16446810.1590/1678-5150-PVB-5388S0100-736X2018000500795WOS:000440153300001S0100-736X2018000500795.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2023-10-23T06:10:18Zoai:repositorio.unesp.br:11449/164468Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:45:43.565749Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Proteinogram and serum IgG concentration in newborn foals up to thirty days of life treated with plasma |
title |
Proteinogram and serum IgG concentration in newborn foals up to thirty days of life treated with plasma |
spellingShingle |
Proteinogram and serum IgG concentration in newborn foals up to thirty days of life treated with plasma Neto, Campo A. V. C. [UNESP] Proteinogram IgG foals plasma equine enzyme immunoassay electrophoresis immunoglobulins proteins |
title_short |
Proteinogram and serum IgG concentration in newborn foals up to thirty days of life treated with plasma |
title_full |
Proteinogram and serum IgG concentration in newborn foals up to thirty days of life treated with plasma |
title_fullStr |
Proteinogram and serum IgG concentration in newborn foals up to thirty days of life treated with plasma |
title_full_unstemmed |
Proteinogram and serum IgG concentration in newborn foals up to thirty days of life treated with plasma |
title_sort |
Proteinogram and serum IgG concentration in newborn foals up to thirty days of life treated with plasma |
author |
Neto, Campo A. V. C. [UNESP] |
author_facet |
Neto, Campo A. V. C. [UNESP] Oliveira-Filho, Jose P. [UNESP] Delfiol, Diego J. Z. Badial, Peres R. Araujo Junior, Joao P. [UNESP] Cruz, Tais E. [UNESP] Tenorio, Michely S. [UNESP] Borges, Alexandre S. [UNESP] |
author_role |
author |
author2 |
Oliveira-Filho, Jose P. [UNESP] Delfiol, Diego J. Z. Badial, Peres R. Araujo Junior, Joao P. [UNESP] Cruz, Tais E. [UNESP] Tenorio, Michely S. [UNESP] Borges, Alexandre S. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Uberlândia (UFU) Mississippi State Univ |
dc.contributor.author.fl_str_mv |
Neto, Campo A. V. C. [UNESP] Oliveira-Filho, Jose P. [UNESP] Delfiol, Diego J. Z. Badial, Peres R. Araujo Junior, Joao P. [UNESP] Cruz, Tais E. [UNESP] Tenorio, Michely S. [UNESP] Borges, Alexandre S. [UNESP] |
dc.subject.por.fl_str_mv |
Proteinogram IgG foals plasma equine enzyme immunoassay electrophoresis immunoglobulins proteins |
topic |
Proteinogram IgG foals plasma equine enzyme immunoassay electrophoresis immunoglobulins proteins |
description |
The aim or this study was to evaluate serum protein ana serum IgG concentrations [after a direct enzyme immunoassay test ELISA optimization) in newborns foals from birth to thirty days of life before and after colostrum consumption and intravenous treatment with plasma. Twenty foals and their respective progenitors as well as four plasma donor's horses were used. Blood samples were obtained from newborn foals at five time points, immediately after birth and before colostrum intake (Ml), ten hours after birth (M2), 24 hours after birth and prior administration of blood plasma (M3), 48 hours after birth and 24 hours after plasma administration (M4), and 30 days after birth (M5). Blood and colostrum samples were collected from the progenitor mares immediately postpartum. Concentration of total protein (TP) and albumin were determined using a biochemical analyzer. The TP concentration was also measured by refractometer. Fractions of total serum protein were separated using agarose gel electrophoresis. Colostrum density was evaluated using BRIX refractometer and specific density colostrometer. Total IgG concentration was determined by an enzyme-linked immunosorbent assay. With the ELISA system proposed here it was possible to determine IgG concentrations in serum, plasma, and equine colostrum samples with adequate repeatability. Serum IgG concentration in foals at birth was 15 +/- 8mg/dL (mean +/- standard deviation) raising at ten hours (2,408 +/- 608mg/dL) and remaining at similar levels up to 48 hours of life (2,364 +/- 784m g/dL), and decreasing significantly at 30 days of age (1,414 +/- 586mg/dL). Serum and colostrum IgG concentrations of mares were 1,746 +/- 505mg/dL and 7,714 +/- 2,619mg/dL, respectively. The plasma IgG concentrations from donor mares were 2,026 +/- 148mg/dL. Total protein, total globulins, and gamma-globulin fraction showed correlation with IgG. Ten hours post birth was an adequate time to verify the transfer of passive immunity, allowing to adoption prophylactic and/or therapeutic measures in a horse farms. One liter of plasma administered at 24 hours of life was not sufficient to raise serum IgG concentrations in foals without passive immunity transfer failure. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:54:40Z 2018-11-26T17:54:40Z 2018-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-5150-PVB-5388 Pesquisa Veterinaria Brasileira. Rio Janeiro: Revista Pesquisa Veterinaria Brasileira, v. 38, n. 5, p. 795-805, 2018. 0100-736X http://hdl.handle.net/11449/164468 10.1590/1678-5150-PVB-5388 S0100-736X2018000500795 WOS:000440153300001 S0100-736X2018000500795.pdf |
url |
http://dx.doi.org/10.1590/1678-5150-PVB-5388 http://hdl.handle.net/11449/164468 |
identifier_str_mv |
Pesquisa Veterinaria Brasileira. Rio Janeiro: Revista Pesquisa Veterinaria Brasileira, v. 38, n. 5, p. 795-805, 2018. 0100-736X 10.1590/1678-5150-PVB-5388 S0100-736X2018000500795 WOS:000440153300001 S0100-736X2018000500795.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Pesquisa Veterinaria Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
795-805 application/pdf |
dc.publisher.none.fl_str_mv |
Revista Pesquisa Veterinaria Brasileira |
publisher.none.fl_str_mv |
Revista Pesquisa Veterinaria Brasileira |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128222329569280 |