Análise dos indicadores da osteogênese, citotoxicidade e genotoxicidade de substitutos ósseos em osteoblastos-like

• Autor(a) principal: Milhan, Noala Vicensoto Moreira [UNESP]
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/108851
• topic: Hidroxiapatita; Fosfatos; Osteoblastos; Citotoxicidade

The aim of this study was to investigate in vitro the indicators of osteogenesis, the cytotoxicity and genotoxicity of osteoblast-like (MG 63) in indirect contact with three bone substitutes: Hydroxypatite Bovine (HA) (Bio -Oss ® - Geistlich), Beta Tricalcium Phosphate (β -TCP - Bionnovation) and experimental Beta Tricalcium Phosphate (β -TCP experimental). The osteoblasts-like were exposed to the materials for 24 h, through the use of conditioned medium by bone substitutes in the concentration of 16 mg/mL. The indicators of osteogenesis were analyzed by alkaline phosphatase activity, total protein content and formation of mineralized nodules. Cell viability was measured by MTT assay and genotoxicity was determined by the formation of micronuclei (MNT) in the cells after exposure to the bone substitute and also to the etilmetano sulfanato (positive control). The data obtained were submitted to ANOVA, Tukey's multiple comparison (Post Hoc) and Z test (p < 0,05 ). The MTT assay showed that none of the tested materials was cytotoxic. Furthemore, the β -TCP, followed by Bio- Oss® , induced proliferation of cells after 24 h. The MNT test indicated that none of the bone substitutes was genotoxic. Concerning the indicators of osteogenesis, it was observed that cells in contact with the materials were able to induce osteogenesis and that this process was influenced by the period of culture of osteoblast-like in contact with bone substitutes. The total protein content was the highest in general on the 10th day, while the alkaline phosphatase activity was the highest in all the materials on the 14th day, similar to the formation of mineralized nodules, which also showed the highest values on the 14th day. Among the evaluated bone substitutes, it was found that the β-TCP induced higher osteogenesis than others on all the tests. Based on the results of this study, it was concluded that all.....