Research of Klebsiella pneumoniae in dairy herds

Detalhes bibliográficos
Autor(a) principal: Langoni, Helio [UNESP]
Data de Publicação: 2015
Outros Autores: Guiduce, Marcos Vinicius S. [UNESP], Nobrega, Diego B. [UNESP], Silva, Rodrigo C. da [UNESP], Richini-Pereira, Virginia B. [UNESP], Salina, Anelise [UNESP], Guimaraes, Felipe de F. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.scielo.br/scielo.php?pid=S0100-736X2015000100009&script=sci_arttext
http://hdl.handle.net/11449/128429
Resumo: Klebsiella pneumoniae is a common environmental agent of clinical and subclinical mastitis affecting dairy herds, and may be present in the final product decreasing its quality. Mastitis caused by K. pneumoniae is even more severe due to its poor response to antibiotic therapy, rapid evolution to toxic shock and death of the animal. This paper aimed to study the prevalence of this pathogen among dairy herds in ten farms located in different municipalities of Sao Paulo State based on size and use of milking technology. All mammary glands of all lactating cows were screened using the California Mastitis Test (CMT) and a strip cup. A single aseptic milk sample (20mL) was collected from all CMT-positive quarters and bulk tanks, whereas swab samples were collected from feces, hind limbs of the animals, bedding and milking parlor. Identification of K. pneumoniae was performed using conventional microbiology culture, biochemical assay and Polimerase Chain Reaction. The primers were designed and tested at the Laboratory of Molecular Biology applied to Zoonoses (FMVZ, Unesp-Botucatu) targeting the 16S rRNA gene. This study included 1067 animals. Six cases of intramammary infection by K. pneumoniae were detected in six different cows in two farms. Moreover, K. pneumoniae was isolated in 77 swabs (34 from bedding in 9 farms, 7 from waiting rooms in 5 farms, 6 from milking parlors in 4 farms, 11 from rectums in six farms, and 19 from hindlimbs in 7 farms. Molecular analysis confirmed the agent was K. pneumoniae. At least one strain of the agent was identified in a certain site in all farms, showing the need of maintaining the hygiene in dairy farms.
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spelling Research of Klebsiella pneumoniae in dairy herdsPesquisa de Klebsiella pneumoniae em rebanhos leiteirosEnvironmentKlebsiella pneumoniaeMilkMastitisDairy herdsAmbientalKlebsiella pneumoniaeLeiteMastiteBovinosKlebsiella pneumoniae is a common environmental agent of clinical and subclinical mastitis affecting dairy herds, and may be present in the final product decreasing its quality. Mastitis caused by K. pneumoniae is even more severe due to its poor response to antibiotic therapy, rapid evolution to toxic shock and death of the animal. This paper aimed to study the prevalence of this pathogen among dairy herds in ten farms located in different municipalities of Sao Paulo State based on size and use of milking technology. All mammary glands of all lactating cows were screened using the California Mastitis Test (CMT) and a strip cup. A single aseptic milk sample (20mL) was collected from all CMT-positive quarters and bulk tanks, whereas swab samples were collected from feces, hind limbs of the animals, bedding and milking parlor. Identification of K. pneumoniae was performed using conventional microbiology culture, biochemical assay and Polimerase Chain Reaction. The primers were designed and tested at the Laboratory of Molecular Biology applied to Zoonoses (FMVZ, Unesp-Botucatu) targeting the 16S rRNA gene. This study included 1067 animals. Six cases of intramammary infection by K. pneumoniae were detected in six different cows in two farms. Moreover, K. pneumoniae was isolated in 77 swabs (34 from bedding in 9 farms, 7 from waiting rooms in 5 farms, 6 from milking parlors in 4 farms, 11 from rectums in six farms, and 19 from hindlimbs in 7 farms. Molecular analysis confirmed the agent was K. pneumoniae. At least one strain of the agent was identified in a certain site in all farms, showing the need of maintaining the hygiene in dairy farms.Klebsiella pneumoniae é um agente ambiental comum de mastite clínica e subclínica que afetam vacas leiteiras e pode estar presente no produto final, reduzindo a sua qualidade. Mastite causada por K. pneumoniae é ainda mais grave devido à sua má resposta à antibioticoterapia, rápida evolução para choque tóxico e morte do animal. Este trabalho teve como objetivo estudar a prevalência deste patógeno entre os rebanhos leiteiros em dez fazendas localizadas em diferentes municípios do Estado de São Paulo com base no tamanho do rebanho e uso de tecnologia de ordenha. Todas as glândulas mamárias das vacas em lactação foram examinadas usando o California Mastitis Test (CMT) e caneca de fundo telado. Foram colhidas amostras de leite (20mL) de todos os quartos CMT- positivos e dos tanques de expansão, também foram colhidos swab de fezes, membros posteriores dos animais, cama dos animais e sala de ordenha. O isolamento e identificação de K. pneumoniae foi realizada através de cultura microbiológica convencional, ensaio bioquímico e Reação em Cadeia da Polimerase, utilizando primers desenhados e testados no Laboratório de Biologia Molecular aplicada à Zoonoses (FMVZ, Unesp-Botucatu) com base na região do gene de 16S rRNA. Este estudo incluiu 1067 animais. Foram detectados seis casos de infecção intramamária por K. pneumoniae em seis diferentes animais em duas fazendas. Ainda, K. pneumoniae foi isolada em 77 swabs (34 de camas em 9 propriedades, 7 de salas de pré-ordenha em 5 propriedades, 6 de salas de ordenha em 4 propriedades, 11 do reto de animais em 6 propriedades e 19 de membros posteriores em 7 propriedades. A análise molecular confirmou o agente K. pneumoniae. K. pneumoniae foi isolada pelo menos em uma localização em todas as propriedades leiteiras., salientando a necessidade de manter a higiene nas fazendas leiteiras a fim de controlar a mastite por esse patógeno.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, UNESP, Dept Vet Hyg &Publ Hlth, BR-18618970 Botucatu, SP, BrazilSao Paulo State Univ, UNESP, Dept Vet Hyg & Publ Hlth, BR-18618970 Botucatu, SP, BrazilFAPESP: 2009/10864-7Revista Pesquisa Veterinaria BrasileiraUniversidade Estadual Paulista (Unesp)Langoni, Helio [UNESP]Guiduce, Marcos Vinicius S. [UNESP]Nobrega, Diego B. [UNESP]Silva, Rodrigo C. da [UNESP]Richini-Pereira, Virginia B. [UNESP]Salina, Anelise [UNESP]Guimaraes, Felipe de F. [UNESP]2015-10-21T13:09:47Z2015-10-21T13:09:47Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9-12application/pdfhttp://www.scielo.br/scielo.php?pid=S0100-736X2015000100009&script=sci_arttextPesquisa Veterinaria Brasileira, v. 35, n. 1, p. 9-12, 2015.0100-736Xhttp://hdl.handle.net/11449/12842910.1590/S0100-736X2015000100003S0100-736X2015000100009WOS:000353792000003S0100-736X2015000100009.pdf5326072118518067Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinaria Brasileira0.385info:eu-repo/semantics/openAccess2023-12-18T06:13:08Zoai:repositorio.unesp.br:11449/128429Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:36:51.787899Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Research of Klebsiella pneumoniae in dairy herds
Pesquisa de Klebsiella pneumoniae em rebanhos leiteiros
title Research of Klebsiella pneumoniae in dairy herds
spellingShingle Research of Klebsiella pneumoniae in dairy herds
Langoni, Helio [UNESP]
Environment
Klebsiella pneumoniae
Milk
Mastitis
Dairy herds
Ambiental
Klebsiella pneumoniae
Leite
Mastite
Bovinos
title_short Research of Klebsiella pneumoniae in dairy herds
title_full Research of Klebsiella pneumoniae in dairy herds
title_fullStr Research of Klebsiella pneumoniae in dairy herds
title_full_unstemmed Research of Klebsiella pneumoniae in dairy herds
title_sort Research of Klebsiella pneumoniae in dairy herds
author Langoni, Helio [UNESP]
author_facet Langoni, Helio [UNESP]
Guiduce, Marcos Vinicius S. [UNESP]
Nobrega, Diego B. [UNESP]
Silva, Rodrigo C. da [UNESP]
Richini-Pereira, Virginia B. [UNESP]
Salina, Anelise [UNESP]
Guimaraes, Felipe de F. [UNESP]
author_role author
author2 Guiduce, Marcos Vinicius S. [UNESP]
Nobrega, Diego B. [UNESP]
Silva, Rodrigo C. da [UNESP]
Richini-Pereira, Virginia B. [UNESP]
Salina, Anelise [UNESP]
Guimaraes, Felipe de F. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Langoni, Helio [UNESP]
Guiduce, Marcos Vinicius S. [UNESP]
Nobrega, Diego B. [UNESP]
Silva, Rodrigo C. da [UNESP]
Richini-Pereira, Virginia B. [UNESP]
Salina, Anelise [UNESP]
Guimaraes, Felipe de F. [UNESP]
dc.subject.por.fl_str_mv Environment
Klebsiella pneumoniae
Milk
Mastitis
Dairy herds
Ambiental
Klebsiella pneumoniae
Leite
Mastite
Bovinos
topic Environment
Klebsiella pneumoniae
Milk
Mastitis
Dairy herds
Ambiental
Klebsiella pneumoniae
Leite
Mastite
Bovinos
description Klebsiella pneumoniae is a common environmental agent of clinical and subclinical mastitis affecting dairy herds, and may be present in the final product decreasing its quality. Mastitis caused by K. pneumoniae is even more severe due to its poor response to antibiotic therapy, rapid evolution to toxic shock and death of the animal. This paper aimed to study the prevalence of this pathogen among dairy herds in ten farms located in different municipalities of Sao Paulo State based on size and use of milking technology. All mammary glands of all lactating cows were screened using the California Mastitis Test (CMT) and a strip cup. A single aseptic milk sample (20mL) was collected from all CMT-positive quarters and bulk tanks, whereas swab samples were collected from feces, hind limbs of the animals, bedding and milking parlor. Identification of K. pneumoniae was performed using conventional microbiology culture, biochemical assay and Polimerase Chain Reaction. The primers were designed and tested at the Laboratory of Molecular Biology applied to Zoonoses (FMVZ, Unesp-Botucatu) targeting the 16S rRNA gene. This study included 1067 animals. Six cases of intramammary infection by K. pneumoniae were detected in six different cows in two farms. Moreover, K. pneumoniae was isolated in 77 swabs (34 from bedding in 9 farms, 7 from waiting rooms in 5 farms, 6 from milking parlors in 4 farms, 11 from rectums in six farms, and 19 from hindlimbs in 7 farms. Molecular analysis confirmed the agent was K. pneumoniae. At least one strain of the agent was identified in a certain site in all farms, showing the need of maintaining the hygiene in dairy farms.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-21T13:09:47Z
2015-10-21T13:09:47Z
2015-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?pid=S0100-736X2015000100009&script=sci_arttext
Pesquisa Veterinaria Brasileira, v. 35, n. 1, p. 9-12, 2015.
0100-736X
http://hdl.handle.net/11449/128429
10.1590/S0100-736X2015000100003
S0100-736X2015000100009
WOS:000353792000003
S0100-736X2015000100009.pdf
5326072118518067
url http://www.scielo.br/scielo.php?pid=S0100-736X2015000100009&script=sci_arttext
http://hdl.handle.net/11449/128429
identifier_str_mv Pesquisa Veterinaria Brasileira, v. 35, n. 1, p. 9-12, 2015.
0100-736X
10.1590/S0100-736X2015000100003
S0100-736X2015000100009
WOS:000353792000003
S0100-736X2015000100009.pdf
5326072118518067
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pesquisa Veterinaria Brasileira
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dc.format.none.fl_str_mv 9-12
application/pdf
dc.publisher.none.fl_str_mv Revista Pesquisa Veterinaria Brasileira
publisher.none.fl_str_mv Revista Pesquisa Veterinaria Brasileira
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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