Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo7_v9_n4.pdf http://hdl.handle.net/11449/69965 |
Resumo: | Preliminary pharmacological assays of the 70% methanol extract from the leaves of the Brazilian medicinal plant Zollernia ilicifolia Vog. (Fabaceae) showed analgesic and antiulcerogenic effects. Previous analyses have shown that this extract contains, besides flavonoid glycosides and saponins, a toxic cyanogenic glycoside. Flavonoids and saponins are compounds reported in literature with antiulcerogenic activity. In this work, we developed a methodology to separate the cyanogenic glycoside from these compounds in order to obtain enough amount of material to perform pharmacological assays. The cyanogenic glycoside zierin (2S)-β-D-glucopyranosyloxy-(3-hydroxy-phenyl)- acetonitrile was separated from the other components by high speed countercurrent chromatography (HSCCC). The solvent system used was composed of chloroform-methanol-n-propanol-water (5:6:1:4, v/v/v/v). This technique led to the separation of zierin from the possible active compounds of Zollernia ilicifolia. |
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Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatographyHigh-speed counter-current chromatographyZierinZollernia ilicifoliachloroformflavonoidglycosidemethanolplant extractpropanolsaponin derivativesolventunclassified drugwaterzierinzollernia ilicifolia extractchromatographydrug structurehigh performance liquid chromatographyhigh speed countercurrent chromatographylegumemedicinal plantseparation techniquethin layer chromatographyFabaceaeZollerniaPreliminary pharmacological assays of the 70% methanol extract from the leaves of the Brazilian medicinal plant Zollernia ilicifolia Vog. (Fabaceae) showed analgesic and antiulcerogenic effects. Previous analyses have shown that this extract contains, besides flavonoid glycosides and saponins, a toxic cyanogenic glycoside. Flavonoids and saponins are compounds reported in literature with antiulcerogenic activity. In this work, we developed a methodology to separate the cyanogenic glycoside from these compounds in order to obtain enough amount of material to perform pharmacological assays. The cyanogenic glycoside zierin (2S)-β-D-glucopyranosyloxy-(3-hydroxy-phenyl)- acetonitrile was separated from the other components by high speed countercurrent chromatography (HSCCC). The solvent system used was composed of chloroform-methanol-n-propanol-water (5:6:1:4, v/v/v/v). This technique led to the separation of zierin from the possible active compounds of Zollernia ilicifolia.Departamento de Química Orgânica Instituto de Química Universidade Estadual Paulista, CP 355, CEP 14801-970, Araraquara, SPDepartamento de Farmacologia Instituto de Biociências Universidade Estadual Paulista, 18618-000, Botucatu, SPDepartamento de Química Orgânica Instituto de Química Universidade Estadual Paulista, CP 355, CEP 14801-970, Araraquara, SPDepartamento de Farmacologia Instituto de Biociências Universidade Estadual Paulista, 18618-000, Botucatu, SPUniversidade Estadual Paulista (Unesp)Coelho, R. G. [UNESP]Calvo, T. R. [UNESP]Santos, Lourdes Campaner dos [UNESP]Di Stasi, L. C. [UNESP]Vilegas, Wagner [UNESP]2014-05-27T11:22:38Z2014-05-27T11:22:38Z2007-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article39-43application/pdfhttp://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo7_v9_n4.pdfRevista Brasileira de Plantas Medicinais, v. 9, n. 4, p. 39-43, 2007.1516-0572http://hdl.handle.net/11449/699652-s2.0-385491647862-s2.0-38549164786.pdf1697547325096457353825364060297735382536406029770000-0003-3032-2556Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Plantas Medicinais0,199info:eu-repo/semantics/openAccess2024-01-03T06:25:21Zoai:repositorio.unesp.br:11449/69965Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:02:42.910894Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography |
title |
Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography |
spellingShingle |
Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography Coelho, R. G. [UNESP] High-speed counter-current chromatography Zierin Zollernia ilicifolia chloroform flavonoid glycoside methanol plant extract propanol saponin derivative solvent unclassified drug water zierin zollernia ilicifolia extract chromatography drug structure high performance liquid chromatography high speed countercurrent chromatography legume medicinal plant separation technique thin layer chromatography Fabaceae Zollernia |
title_short |
Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography |
title_full |
Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography |
title_fullStr |
Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography |
title_full_unstemmed |
Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography |
title_sort |
Separation of the toxic zierin from Zollernia ilicifolia by high speed countercurrent chromatography |
author |
Coelho, R. G. [UNESP] |
author_facet |
Coelho, R. G. [UNESP] Calvo, T. R. [UNESP] Santos, Lourdes Campaner dos [UNESP] Di Stasi, L. C. [UNESP] Vilegas, Wagner [UNESP] |
author_role |
author |
author2 |
Calvo, T. R. [UNESP] Santos, Lourdes Campaner dos [UNESP] Di Stasi, L. C. [UNESP] Vilegas, Wagner [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Coelho, R. G. [UNESP] Calvo, T. R. [UNESP] Santos, Lourdes Campaner dos [UNESP] Di Stasi, L. C. [UNESP] Vilegas, Wagner [UNESP] |
dc.subject.por.fl_str_mv |
High-speed counter-current chromatography Zierin Zollernia ilicifolia chloroform flavonoid glycoside methanol plant extract propanol saponin derivative solvent unclassified drug water zierin zollernia ilicifolia extract chromatography drug structure high performance liquid chromatography high speed countercurrent chromatography legume medicinal plant separation technique thin layer chromatography Fabaceae Zollernia |
topic |
High-speed counter-current chromatography Zierin Zollernia ilicifolia chloroform flavonoid glycoside methanol plant extract propanol saponin derivative solvent unclassified drug water zierin zollernia ilicifolia extract chromatography drug structure high performance liquid chromatography high speed countercurrent chromatography legume medicinal plant separation technique thin layer chromatography Fabaceae Zollernia |
description |
Preliminary pharmacological assays of the 70% methanol extract from the leaves of the Brazilian medicinal plant Zollernia ilicifolia Vog. (Fabaceae) showed analgesic and antiulcerogenic effects. Previous analyses have shown that this extract contains, besides flavonoid glycosides and saponins, a toxic cyanogenic glycoside. Flavonoids and saponins are compounds reported in literature with antiulcerogenic activity. In this work, we developed a methodology to separate the cyanogenic glycoside from these compounds in order to obtain enough amount of material to perform pharmacological assays. The cyanogenic glycoside zierin (2S)-β-D-glucopyranosyloxy-(3-hydroxy-phenyl)- acetonitrile was separated from the other components by high speed countercurrent chromatography (HSCCC). The solvent system used was composed of chloroform-methanol-n-propanol-water (5:6:1:4, v/v/v/v). This technique led to the separation of zierin from the possible active compounds of Zollernia ilicifolia. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-11-01 2014-05-27T11:22:38Z 2014-05-27T11:22:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo7_v9_n4.pdf Revista Brasileira de Plantas Medicinais, v. 9, n. 4, p. 39-43, 2007. 1516-0572 http://hdl.handle.net/11449/69965 2-s2.0-38549164786 2-s2.0-38549164786.pdf 1697547325096457 3538253640602977 3538253640602977 0000-0003-3032-2556 |
url |
http://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo7_v9_n4.pdf http://hdl.handle.net/11449/69965 |
identifier_str_mv |
Revista Brasileira de Plantas Medicinais, v. 9, n. 4, p. 39-43, 2007. 1516-0572 2-s2.0-38549164786 2-s2.0-38549164786.pdf 1697547325096457 3538253640602977 0000-0003-3032-2556 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Plantas Medicinais 0,199 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
39-43 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129386460741632 |