Toxicity evaluation of vinasse and biosolid samples in diplopod midgut: heat shock protein in situ localization
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11356-017-9754-2 http://hdl.handle.net/11449/179082 |
Resumo: | Large amounts of residues generated by agricultural, urban and industrial activities are dumped daily on the soil. This practice deserves special attention because it causes serious environmental problems. This study evaluated the toxic potential of the sugarcane vinasse, a by-product of the sugar-alcohol industry, and the biosolid, a residue produced by wastewater treatment plants, both widely used as fertilizers. The evaluation was performed through bioassays using a typical soil bioindicator, the diplopod Rhinocricus padbergi. The specimens were exposed to soils containing these residues in concentrations that are compatible with the Brazilian regulation for agricultural use. Semi-quantitative immunolabelling analyses of the stress protein HSP70 were performed on the midgut of the studied diplopods. There was a significant increase in the immunolabelling of HSP70 proteins as a response to xenobiotics from both residues, particularly in regions where the function of the cells is the detoxification of the organ (e.g. the hepatic cell layer and specific regions of the epithelium). Higher immunolabelling was observed in the specimens exposed to vinasse in comparison with the biosolid exposure. This demonstrates that the substances in the tested residues had proteotoxic action in the exposed animals and induced a cytoprotective response, which led to higher stress protein immunolabelling. Therefore, caution is needed for the use of such residues in agriculture. |
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Toxicity evaluation of vinasse and biosolid samples in diplopod midgut: heat shock protein in situ localizationFertilizersHSP70ImmunohistochemistryMillipedesR. padbergiSoil toxicityLarge amounts of residues generated by agricultural, urban and industrial activities are dumped daily on the soil. This practice deserves special attention because it causes serious environmental problems. This study evaluated the toxic potential of the sugarcane vinasse, a by-product of the sugar-alcohol industry, and the biosolid, a residue produced by wastewater treatment plants, both widely used as fertilizers. The evaluation was performed through bioassays using a typical soil bioindicator, the diplopod Rhinocricus padbergi. The specimens were exposed to soils containing these residues in concentrations that are compatible with the Brazilian regulation for agricultural use. Semi-quantitative immunolabelling analyses of the stress protein HSP70 were performed on the midgut of the studied diplopods. There was a significant increase in the immunolabelling of HSP70 proteins as a response to xenobiotics from both residues, particularly in regions where the function of the cells is the detoxification of the organ (e.g. the hepatic cell layer and specific regions of the epithelium). Higher immunolabelling was observed in the specimens exposed to vinasse in comparison with the biosolid exposure. This demonstrates that the substances in the tested residues had proteotoxic action in the exposed animals and induced a cytoprotective response, which led to higher stress protein immunolabelling. Therefore, caution is needed for the use of such residues in agriculture.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biology Biosciences Institute UNESP (São Paulo State University), Av. 24-A, 1515Department of Biology CCHB UFSCar (Universidade Federal de São Carlos)Department of Biology Biosciences Institute UNESP (São Paulo State University), Av. 24-A, 1515FAPESP: 2012/50197-2FAPESP: 2014/12646-5Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Coelho, Maria Paula Mancini [UNESP]Moreira-de-Sousa, Cristina [UNESP]de Souza, Raphael Bastão [UNESP]Ansoar-Rodríguez, Yadira [UNESP]Silva-Zacarin, Elaine Cristina MathiasFontanetti, Carmem Silvia [UNESP]2018-12-11T17:33:38Z2018-12-11T17:33:38Z2017-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article22007-22017application/pdfhttp://dx.doi.org/10.1007/s11356-017-9754-2Environmental Science and Pollution Research, v. 24, n. 27, p. 22007-22017, 2017.1614-74990944-1344http://hdl.handle.net/11449/17908210.1007/s11356-017-9754-22-s2.0-850269130072-s2.0-85026913007.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Science and Pollution Research0,858info:eu-repo/semantics/openAccess2023-11-29T06:15:10Zoai:repositorio.unesp.br:11449/179082Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:04:32.991744Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Toxicity evaluation of vinasse and biosolid samples in diplopod midgut: heat shock protein in situ localization |
title |
Toxicity evaluation of vinasse and biosolid samples in diplopod midgut: heat shock protein in situ localization |
spellingShingle |
Toxicity evaluation of vinasse and biosolid samples in diplopod midgut: heat shock protein in situ localization Coelho, Maria Paula Mancini [UNESP] Fertilizers HSP70 Immunohistochemistry Millipedes R. padbergi Soil toxicity |
title_short |
Toxicity evaluation of vinasse and biosolid samples in diplopod midgut: heat shock protein in situ localization |
title_full |
Toxicity evaluation of vinasse and biosolid samples in diplopod midgut: heat shock protein in situ localization |
title_fullStr |
Toxicity evaluation of vinasse and biosolid samples in diplopod midgut: heat shock protein in situ localization |
title_full_unstemmed |
Toxicity evaluation of vinasse and biosolid samples in diplopod midgut: heat shock protein in situ localization |
title_sort |
Toxicity evaluation of vinasse and biosolid samples in diplopod midgut: heat shock protein in situ localization |
author |
Coelho, Maria Paula Mancini [UNESP] |
author_facet |
Coelho, Maria Paula Mancini [UNESP] Moreira-de-Sousa, Cristina [UNESP] de Souza, Raphael Bastão [UNESP] Ansoar-Rodríguez, Yadira [UNESP] Silva-Zacarin, Elaine Cristina Mathias Fontanetti, Carmem Silvia [UNESP] |
author_role |
author |
author2 |
Moreira-de-Sousa, Cristina [UNESP] de Souza, Raphael Bastão [UNESP] Ansoar-Rodríguez, Yadira [UNESP] Silva-Zacarin, Elaine Cristina Mathias Fontanetti, Carmem Silvia [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Coelho, Maria Paula Mancini [UNESP] Moreira-de-Sousa, Cristina [UNESP] de Souza, Raphael Bastão [UNESP] Ansoar-Rodríguez, Yadira [UNESP] Silva-Zacarin, Elaine Cristina Mathias Fontanetti, Carmem Silvia [UNESP] |
dc.subject.por.fl_str_mv |
Fertilizers HSP70 Immunohistochemistry Millipedes R. padbergi Soil toxicity |
topic |
Fertilizers HSP70 Immunohistochemistry Millipedes R. padbergi Soil toxicity |
description |
Large amounts of residues generated by agricultural, urban and industrial activities are dumped daily on the soil. This practice deserves special attention because it causes serious environmental problems. This study evaluated the toxic potential of the sugarcane vinasse, a by-product of the sugar-alcohol industry, and the biosolid, a residue produced by wastewater treatment plants, both widely used as fertilizers. The evaluation was performed through bioassays using a typical soil bioindicator, the diplopod Rhinocricus padbergi. The specimens were exposed to soils containing these residues in concentrations that are compatible with the Brazilian regulation for agricultural use. Semi-quantitative immunolabelling analyses of the stress protein HSP70 were performed on the midgut of the studied diplopods. There was a significant increase in the immunolabelling of HSP70 proteins as a response to xenobiotics from both residues, particularly in regions where the function of the cells is the detoxification of the organ (e.g. the hepatic cell layer and specific regions of the epithelium). Higher immunolabelling was observed in the specimens exposed to vinasse in comparison with the biosolid exposure. This demonstrates that the substances in the tested residues had proteotoxic action in the exposed animals and induced a cytoprotective response, which led to higher stress protein immunolabelling. Therefore, caution is needed for the use of such residues in agriculture. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-01 2018-12-11T17:33:38Z 2018-12-11T17:33:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11356-017-9754-2 Environmental Science and Pollution Research, v. 24, n. 27, p. 22007-22017, 2017. 1614-7499 0944-1344 http://hdl.handle.net/11449/179082 10.1007/s11356-017-9754-2 2-s2.0-85026913007 2-s2.0-85026913007.pdf |
url |
http://dx.doi.org/10.1007/s11356-017-9754-2 http://hdl.handle.net/11449/179082 |
identifier_str_mv |
Environmental Science and Pollution Research, v. 24, n. 27, p. 22007-22017, 2017. 1614-7499 0944-1344 10.1007/s11356-017-9754-2 2-s2.0-85026913007 2-s2.0-85026913007.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Science and Pollution Research 0,858 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
22007-22017 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129015665393664 |