CXCL1, CCL2, and CCL5 modulation by microbial and biomechanical signals in periodontal cells and tissues—in vitro and in vivo studies
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-020-03244-1 http://hdl.handle.net/11449/200130 |
Resumo: | Objectives: This study was established to investigate whether the chemokines CXCL1, CCL2, and CCL5 are produced in periodontal cells and tissues and, if so, whether their levels are regulated by microbial and/or mechanical signals. Materials and methods: The chemokine expression and protein levels in gingival biopsies from patients with and without periodontitis were analyzed by RT-PCR and immunohistochemistry. The chemokines were also analyzed in gingival biopsies from rats subjected to experimental periodontitis and/or orthodontic tooth movement. Additionally, chemokine levels were determined in periodontal fibroblasts exposed to the periodontopathogen Fusobacterium nucleatum and mechanical forces by RT-PCR and ELISA. Results: Higher CXCL1, CCL2, and CCL5 levels were found in human and rat gingiva from sites of periodontitis as compared with periodontally healthy sites. In the rat experimental periodontitis model, the bacteria-induced upregulation of these chemokines was significantly counteracted by orthodontic forces. In vitro, F. nucleatum caused a significant upregulation of all chemokines at 1 day. When the cells were subjected simultaneously to F. nucleatum and mechanical forces, the upregulation of chemokines was significantly inhibited. The transcriptional findings were paralleled at protein level. Conclusions: This study provides original evidence in vitro and in vivo that the chemokines CXCL1, CCL2, and CCL5 are regulated by both microbial and mechanical signals in periodontal cells and tissues. Furthermore, our study revealed that biomechanical forces can counteract the stimulatory actions of F. nucleatum on these chemokines. Clinical relevance: Mechanical loading might aggravate periodontal infection by compromising the recruitment of immunoinflammatory cells. |
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CXCL1, CCL2, and CCL5 modulation by microbial and biomechanical signals in periodontal cells and tissues—in vitro and in vivo studiesFusobacterium nucleatumGingivitisOrthodontic tooth movementPeriodontitisPeriodontiumObjectives: This study was established to investigate whether the chemokines CXCL1, CCL2, and CCL5 are produced in periodontal cells and tissues and, if so, whether their levels are regulated by microbial and/or mechanical signals. Materials and methods: The chemokine expression and protein levels in gingival biopsies from patients with and without periodontitis were analyzed by RT-PCR and immunohistochemistry. The chemokines were also analyzed in gingival biopsies from rats subjected to experimental periodontitis and/or orthodontic tooth movement. Additionally, chemokine levels were determined in periodontal fibroblasts exposed to the periodontopathogen Fusobacterium nucleatum and mechanical forces by RT-PCR and ELISA. Results: Higher CXCL1, CCL2, and CCL5 levels were found in human and rat gingiva from sites of periodontitis as compared with periodontally healthy sites. In the rat experimental periodontitis model, the bacteria-induced upregulation of these chemokines was significantly counteracted by orthodontic forces. In vitro, F. nucleatum caused a significant upregulation of all chemokines at 1 day. When the cells were subjected simultaneously to F. nucleatum and mechanical forces, the upregulation of chemokines was significantly inhibited. The transcriptional findings were paralleled at protein level. Conclusions: This study provides original evidence in vitro and in vivo that the chemokines CXCL1, CCL2, and CCL5 are regulated by both microbial and mechanical signals in periodontal cells and tissues. Furthermore, our study revealed that biomechanical forces can counteract the stimulatory actions of F. nucleatum on these chemokines. Clinical relevance: Mechanical loading might aggravate periodontal infection by compromising the recruitment of immunoinflammatory cells.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Deutscher Akademischer AustauschdienstCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Deutsche ForschungsgemeinschaftDepartment of Orthodontics Center of Dento-Maxillo-Facial Medicine University of Bonn, Welschnonnenstrasse 17Section of Experimental Dento-Maxillo-Facial Medicine Center of Dento-Maxillo-Facial Medicine University of BonnDepartment of Periodontology and Operative Dentistry University Medical Center of the Johannes Gutenberg UniversityDepartment of Periodontology Laboratory for Oral Microbiology University of BernDepartment of Diagnosis and Surgery School of Dentistry at Araraquara Sao Paulo State University UNESPDepartment of Diagnosis and Surgery School of Dentistry at Araraquara Sao Paulo State University UNESPFAPESP: 2014/20715-7FAPESP: 2017/07137-2Deutscher Akademischer Austauschdienst: 57391253CAPES: 88881.144012/2017-01Deutsche Forschungsgemeinschaft: DE1593/5-1University of BonnUniversity Medical Center of the Johannes Gutenberg UniversityUniversity of BernUniversidade Estadual Paulista (Unesp)Rath-Deschner, BirgitMemmert, SvenjaDamanaki, AnnaNokhbehsaim, MarjanEick, SigrunCirelli, Joni A. [UNESP]Götz, WernerDeschner, JamesJäger, AndreasNogueira, Andressa V. B.2020-12-12T01:58:31Z2020-12-12T01:58:31Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3661-3670http://dx.doi.org/10.1007/s00784-020-03244-1Clinical Oral Investigations, v. 24, n. 10, p. 3661-3670, 2020.1436-37711432-6981http://hdl.handle.net/11449/20013010.1007/s00784-020-03244-12-s2.0-85081038804Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2021-10-23T12:19:10Zoai:repositorio.unesp.br:11449/200130Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:12:10.242438Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
CXCL1, CCL2, and CCL5 modulation by microbial and biomechanical signals in periodontal cells and tissues—in vitro and in vivo studies |
title |
CXCL1, CCL2, and CCL5 modulation by microbial and biomechanical signals in periodontal cells and tissues—in vitro and in vivo studies |
spellingShingle |
CXCL1, CCL2, and CCL5 modulation by microbial and biomechanical signals in periodontal cells and tissues—in vitro and in vivo studies Rath-Deschner, Birgit Fusobacterium nucleatum Gingivitis Orthodontic tooth movement Periodontitis Periodontium |
title_short |
CXCL1, CCL2, and CCL5 modulation by microbial and biomechanical signals in periodontal cells and tissues—in vitro and in vivo studies |
title_full |
CXCL1, CCL2, and CCL5 modulation by microbial and biomechanical signals in periodontal cells and tissues—in vitro and in vivo studies |
title_fullStr |
CXCL1, CCL2, and CCL5 modulation by microbial and biomechanical signals in periodontal cells and tissues—in vitro and in vivo studies |
title_full_unstemmed |
CXCL1, CCL2, and CCL5 modulation by microbial and biomechanical signals in periodontal cells and tissues—in vitro and in vivo studies |
title_sort |
CXCL1, CCL2, and CCL5 modulation by microbial and biomechanical signals in periodontal cells and tissues—in vitro and in vivo studies |
author |
Rath-Deschner, Birgit |
author_facet |
Rath-Deschner, Birgit Memmert, Svenja Damanaki, Anna Nokhbehsaim, Marjan Eick, Sigrun Cirelli, Joni A. [UNESP] Götz, Werner Deschner, James Jäger, Andreas Nogueira, Andressa V. B. |
author_role |
author |
author2 |
Memmert, Svenja Damanaki, Anna Nokhbehsaim, Marjan Eick, Sigrun Cirelli, Joni A. [UNESP] Götz, Werner Deschner, James Jäger, Andreas Nogueira, Andressa V. B. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Bonn University Medical Center of the Johannes Gutenberg University University of Bern Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rath-Deschner, Birgit Memmert, Svenja Damanaki, Anna Nokhbehsaim, Marjan Eick, Sigrun Cirelli, Joni A. [UNESP] Götz, Werner Deschner, James Jäger, Andreas Nogueira, Andressa V. B. |
dc.subject.por.fl_str_mv |
Fusobacterium nucleatum Gingivitis Orthodontic tooth movement Periodontitis Periodontium |
topic |
Fusobacterium nucleatum Gingivitis Orthodontic tooth movement Periodontitis Periodontium |
description |
Objectives: This study was established to investigate whether the chemokines CXCL1, CCL2, and CCL5 are produced in periodontal cells and tissues and, if so, whether their levels are regulated by microbial and/or mechanical signals. Materials and methods: The chemokine expression and protein levels in gingival biopsies from patients with and without periodontitis were analyzed by RT-PCR and immunohistochemistry. The chemokines were also analyzed in gingival biopsies from rats subjected to experimental periodontitis and/or orthodontic tooth movement. Additionally, chemokine levels were determined in periodontal fibroblasts exposed to the periodontopathogen Fusobacterium nucleatum and mechanical forces by RT-PCR and ELISA. Results: Higher CXCL1, CCL2, and CCL5 levels were found in human and rat gingiva from sites of periodontitis as compared with periodontally healthy sites. In the rat experimental periodontitis model, the bacteria-induced upregulation of these chemokines was significantly counteracted by orthodontic forces. In vitro, F. nucleatum caused a significant upregulation of all chemokines at 1 day. When the cells were subjected simultaneously to F. nucleatum and mechanical forces, the upregulation of chemokines was significantly inhibited. The transcriptional findings were paralleled at protein level. Conclusions: This study provides original evidence in vitro and in vivo that the chemokines CXCL1, CCL2, and CCL5 are regulated by both microbial and mechanical signals in periodontal cells and tissues. Furthermore, our study revealed that biomechanical forces can counteract the stimulatory actions of F. nucleatum on these chemokines. Clinical relevance: Mechanical loading might aggravate periodontal infection by compromising the recruitment of immunoinflammatory cells. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:58:31Z 2020-12-12T01:58:31Z 2020-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-020-03244-1 Clinical Oral Investigations, v. 24, n. 10, p. 3661-3670, 2020. 1436-3771 1432-6981 http://hdl.handle.net/11449/200130 10.1007/s00784-020-03244-1 2-s2.0-85081038804 |
url |
http://dx.doi.org/10.1007/s00784-020-03244-1 http://hdl.handle.net/11449/200130 |
identifier_str_mv |
Clinical Oral Investigations, v. 24, n. 10, p. 3661-3670, 2020. 1436-3771 1432-6981 10.1007/s00784-020-03244-1 2-s2.0-85081038804 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3661-3670 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129170631294976 |