The Effects of Refrigeration Temperature and Storage Time on Apoptotic Markers in Equine Semen

Detalhes bibliográficos
Autor(a) principal: Freitas-Dell'Aqua, Camila de Paula
Data de Publicação: 2013
Outros Autores: Monteiro, Gabriel Augusto, Dell'aqua Junior, José Antônio [UNESP], Papa, Frederico Ozanam [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jevs.2012.04.011
http://hdl.handle.net/11449/74179
Resumo: Evaluation of the damage caused by the sperm preservation process is crucial to improving fertilization rates. The objective of this study was to evaluate the effects of refrigeration temperature (5°C and 15°C) and storage time (0, 12, 24, 48, and 72 hours) on apoptotic markers in equine semen. Membrane phosphatidylserine translocation index, caspase activation index, and DNA fragmentation index were analyzed using epifluorescence microscopy. Analysis of variance was used for statistical analysis, and Tukey test was used to compare means. The significance level was set at P < .05. The results demonstrated that for transport duration shorter than 24 hours, semen quality was maintained when stored at either 5°C or 15°C. A storage temperature of 5°C should be used when it is necessary to transport semen for longer than 24 hours. There was a significant decrease in semen quality after 48 hours of refrigeration. © 2013 Elsevier Inc.
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spelling The Effects of Refrigeration Temperature and Storage Time on Apoptotic Markers in Equine SemenCaspase activationDNA fragmentationPS translocationSemenStallionEquidaeEvaluation of the damage caused by the sperm preservation process is crucial to improving fertilization rates. The objective of this study was to evaluate the effects of refrigeration temperature (5°C and 15°C) and storage time (0, 12, 24, 48, and 72 hours) on apoptotic markers in equine semen. Membrane phosphatidylserine translocation index, caspase activation index, and DNA fragmentation index were analyzed using epifluorescence microscopy. Analysis of variance was used for statistical analysis, and Tukey test was used to compare means. The significance level was set at P < .05. The results demonstrated that for transport duration shorter than 24 hours, semen quality was maintained when stored at either 5°C or 15°C. A storage temperature of 5°C should be used when it is necessary to transport semen for longer than 24 hours. There was a significant decrease in semen quality after 48 hours of refrigeration. © 2013 Elsevier Inc.Department of Animal Reproduction and Veterinary Radiology College of Veterinary Medicine e Animal Science University of Sao Paulo State, Botucatu, São PauloUniversidade Estadual Paulista (Unesp)Freitas-Dell'Aqua, Camila de PaulaMonteiro, Gabriel AugustoDell'aqua Junior, José Antônio [UNESP]Papa, Frederico Ozanam [UNESP]2014-05-27T11:27:28Z2014-05-27T11:27:28Z2013-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article27-30application/pdfhttp://dx.doi.org/10.1016/j.jevs.2012.04.011Journal of Equine Veterinary Science, v. 33, n. 1, p. 27-30, 2013.0737-0806http://hdl.handle.net/11449/7417910.1016/j.jevs.2012.04.0112-s2.0-848717571672-s2.0-84871757167.pdf0640158639112242Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Equine Veterinary Science0.8800,390info:eu-repo/semantics/openAccess2024-09-09T14:01:40Zoai:repositorio.unesp.br:11449/74179Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:40Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The Effects of Refrigeration Temperature and Storage Time on Apoptotic Markers in Equine Semen
title The Effects of Refrigeration Temperature and Storage Time on Apoptotic Markers in Equine Semen
spellingShingle The Effects of Refrigeration Temperature and Storage Time on Apoptotic Markers in Equine Semen
Freitas-Dell'Aqua, Camila de Paula
Caspase activation
DNA fragmentation
PS translocation
Semen
Stallion
Equidae
title_short The Effects of Refrigeration Temperature and Storage Time on Apoptotic Markers in Equine Semen
title_full The Effects of Refrigeration Temperature and Storage Time on Apoptotic Markers in Equine Semen
title_fullStr The Effects of Refrigeration Temperature and Storage Time on Apoptotic Markers in Equine Semen
title_full_unstemmed The Effects of Refrigeration Temperature and Storage Time on Apoptotic Markers in Equine Semen
title_sort The Effects of Refrigeration Temperature and Storage Time on Apoptotic Markers in Equine Semen
author Freitas-Dell'Aqua, Camila de Paula
author_facet Freitas-Dell'Aqua, Camila de Paula
Monteiro, Gabriel Augusto
Dell'aqua Junior, José Antônio [UNESP]
Papa, Frederico Ozanam [UNESP]
author_role author
author2 Monteiro, Gabriel Augusto
Dell'aqua Junior, José Antônio [UNESP]
Papa, Frederico Ozanam [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Freitas-Dell'Aqua, Camila de Paula
Monteiro, Gabriel Augusto
Dell'aqua Junior, José Antônio [UNESP]
Papa, Frederico Ozanam [UNESP]
dc.subject.por.fl_str_mv Caspase activation
DNA fragmentation
PS translocation
Semen
Stallion
Equidae
topic Caspase activation
DNA fragmentation
PS translocation
Semen
Stallion
Equidae
description Evaluation of the damage caused by the sperm preservation process is crucial to improving fertilization rates. The objective of this study was to evaluate the effects of refrigeration temperature (5°C and 15°C) and storage time (0, 12, 24, 48, and 72 hours) on apoptotic markers in equine semen. Membrane phosphatidylserine translocation index, caspase activation index, and DNA fragmentation index were analyzed using epifluorescence microscopy. Analysis of variance was used for statistical analysis, and Tukey test was used to compare means. The significance level was set at P < .05. The results demonstrated that for transport duration shorter than 24 hours, semen quality was maintained when stored at either 5°C or 15°C. A storage temperature of 5°C should be used when it is necessary to transport semen for longer than 24 hours. There was a significant decrease in semen quality after 48 hours of refrigeration. © 2013 Elsevier Inc.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
2014-05-27T11:27:28Z
2014-05-27T11:27:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jevs.2012.04.011
Journal of Equine Veterinary Science, v. 33, n. 1, p. 27-30, 2013.
0737-0806
http://hdl.handle.net/11449/74179
10.1016/j.jevs.2012.04.011
2-s2.0-84871757167
2-s2.0-84871757167.pdf
0640158639112242
url http://dx.doi.org/10.1016/j.jevs.2012.04.011
http://hdl.handle.net/11449/74179
identifier_str_mv Journal of Equine Veterinary Science, v. 33, n. 1, p. 27-30, 2013.
0737-0806
10.1016/j.jevs.2012.04.011
2-s2.0-84871757167
2-s2.0-84871757167.pdf
0640158639112242
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Equine Veterinary Science
0.880
0,390
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 27-30
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1810021335304241152

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