Enzymatic responses in the head and midgut of Africanized Apis mellifera contaminated with a sublethal concentration of thiamethoxam
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ecoenv.2021.112581 http://hdl.handle.net/11449/233333 |
Resumo: | The increasing use of insecticides, promoted by the intensification of agriculture, has raised concerns about their influence on the decline of bee colonies, which play a fundamental role in pollination. Thus, it is fundamental to elucidate the effects of insecticides on bees. This study investigated the damage caused by a sublethal concentration of thiamethoxam - TMX (0.0227 ng/μL of feed) in the head and midgut of Africanized Apis mellifera, by analyzing the enzymatic biomarkers, oxidative stress, and occurrence of lipid peroxidation. The data showed that the insecticide increased acetylcholinesterase activity (AChE) and glutathione-S-transferase (GST), whereas carboxylesterase (CaE3) activity decreased in the heads. Our results indicate that the antioxidant enzymes were less active in the head because only glutathione peroxidase (GPX) showed alterations. In the midgut, there were no alkaline phosphatase (ALP) or superoxide dismutase (SOD) responses and a decrease in the activity of CaE was observed. Otherwise, there was an increase in GPX, and the TBARS (thiobarbituric acid reactive substances) assay also showed differences in the midgut. The TBARS (thiobarbituric acid reactive substances) assay also showed differences in the midgut. The results showed enzymes such as CaE3, GST, AChE, ALP, SOD, and GPX, as well as the TBARS assay, are useful biomarkers on bees. They may be used in combination as a promising tool for characterizing bee exposure to insecticides. |
id |
UNSP_214cefafd31549ca4ae435ac5a23742e |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/233333 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Enzymatic responses in the head and midgut of Africanized Apis mellifera contaminated with a sublethal concentration of thiamethoxamBiomarkersHoneybeesNeonicotinoidOxidative stressThe increasing use of insecticides, promoted by the intensification of agriculture, has raised concerns about their influence on the decline of bee colonies, which play a fundamental role in pollination. Thus, it is fundamental to elucidate the effects of insecticides on bees. This study investigated the damage caused by a sublethal concentration of thiamethoxam - TMX (0.0227 ng/μL of feed) in the head and midgut of Africanized Apis mellifera, by analyzing the enzymatic biomarkers, oxidative stress, and occurrence of lipid peroxidation. The data showed that the insecticide increased acetylcholinesterase activity (AChE) and glutathione-S-transferase (GST), whereas carboxylesterase (CaE3) activity decreased in the heads. Our results indicate that the antioxidant enzymes were less active in the head because only glutathione peroxidase (GPX) showed alterations. In the midgut, there were no alkaline phosphatase (ALP) or superoxide dismutase (SOD) responses and a decrease in the activity of CaE was observed. Otherwise, there was an increase in GPX, and the TBARS (thiobarbituric acid reactive substances) assay also showed differences in the midgut. The TBARS (thiobarbituric acid reactive substances) assay also showed differences in the midgut. The results showed enzymes such as CaE3, GST, AChE, ALP, SOD, and GPX, as well as the TBARS assay, are useful biomarkers on bees. They may be used in combination as a promising tool for characterizing bee exposure to insecticides.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (Unesp) Institute of Biosciences, Av. 24A, 1515. CEP: 13506-900NUPEFEN – Núcleo de pesquisas em Educação Física Estética e Nutrição Claretiano University Center, Avenida Santo Antônio Maria Claret, 1724. CEP: 13503-257São Paulo State University (Unesp) Institute of Biosciences, Av. 24A, 1515. CEP: 13506-900FAPESP: 2012/13370-8 (TCR)FAPESP: 2013/21833-0 (LM)FAPESP: 2014/23197-7 (PD)FAPESP: 2017/21097-3 (OM)Universidade Estadual Paulista (UNESP)Claretiano University CenterDecio, Pâmela [UNESP]Miotelo, Lucas [UNESP]Pereira, Franco Dani Campos [UNESP]Roat, Thaisa Cristina [UNESP]Marin-Morales, Maria Aparecida [UNESP]Malaspina, Osmar [UNESP]2022-05-01T07:58:46Z2022-05-01T07:58:46Z2021-10-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.ecoenv.2021.112581Ecotoxicology and Environmental Safety, v. 223.1090-24140147-6513http://hdl.handle.net/11449/23333310.1016/j.ecoenv.2021.1125812-s2.0-85111611864Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEcotoxicology and Environmental Safetyinfo:eu-repo/semantics/openAccess2022-05-01T07:58:46Zoai:repositorio.unesp.br:11449/233333Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:55:57.858623Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Enzymatic responses in the head and midgut of Africanized Apis mellifera contaminated with a sublethal concentration of thiamethoxam |
title |
Enzymatic responses in the head and midgut of Africanized Apis mellifera contaminated with a sublethal concentration of thiamethoxam |
spellingShingle |
Enzymatic responses in the head and midgut of Africanized Apis mellifera contaminated with a sublethal concentration of thiamethoxam Decio, Pâmela [UNESP] Biomarkers Honeybees Neonicotinoid Oxidative stress |
title_short |
Enzymatic responses in the head and midgut of Africanized Apis mellifera contaminated with a sublethal concentration of thiamethoxam |
title_full |
Enzymatic responses in the head and midgut of Africanized Apis mellifera contaminated with a sublethal concentration of thiamethoxam |
title_fullStr |
Enzymatic responses in the head and midgut of Africanized Apis mellifera contaminated with a sublethal concentration of thiamethoxam |
title_full_unstemmed |
Enzymatic responses in the head and midgut of Africanized Apis mellifera contaminated with a sublethal concentration of thiamethoxam |
title_sort |
Enzymatic responses in the head and midgut of Africanized Apis mellifera contaminated with a sublethal concentration of thiamethoxam |
author |
Decio, Pâmela [UNESP] |
author_facet |
Decio, Pâmela [UNESP] Miotelo, Lucas [UNESP] Pereira, Franco Dani Campos [UNESP] Roat, Thaisa Cristina [UNESP] Marin-Morales, Maria Aparecida [UNESP] Malaspina, Osmar [UNESP] |
author_role |
author |
author2 |
Miotelo, Lucas [UNESP] Pereira, Franco Dani Campos [UNESP] Roat, Thaisa Cristina [UNESP] Marin-Morales, Maria Aparecida [UNESP] Malaspina, Osmar [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Claretiano University Center |
dc.contributor.author.fl_str_mv |
Decio, Pâmela [UNESP] Miotelo, Lucas [UNESP] Pereira, Franco Dani Campos [UNESP] Roat, Thaisa Cristina [UNESP] Marin-Morales, Maria Aparecida [UNESP] Malaspina, Osmar [UNESP] |
dc.subject.por.fl_str_mv |
Biomarkers Honeybees Neonicotinoid Oxidative stress |
topic |
Biomarkers Honeybees Neonicotinoid Oxidative stress |
description |
The increasing use of insecticides, promoted by the intensification of agriculture, has raised concerns about their influence on the decline of bee colonies, which play a fundamental role in pollination. Thus, it is fundamental to elucidate the effects of insecticides on bees. This study investigated the damage caused by a sublethal concentration of thiamethoxam - TMX (0.0227 ng/μL of feed) in the head and midgut of Africanized Apis mellifera, by analyzing the enzymatic biomarkers, oxidative stress, and occurrence of lipid peroxidation. The data showed that the insecticide increased acetylcholinesterase activity (AChE) and glutathione-S-transferase (GST), whereas carboxylesterase (CaE3) activity decreased in the heads. Our results indicate that the antioxidant enzymes were less active in the head because only glutathione peroxidase (GPX) showed alterations. In the midgut, there were no alkaline phosphatase (ALP) or superoxide dismutase (SOD) responses and a decrease in the activity of CaE was observed. Otherwise, there was an increase in GPX, and the TBARS (thiobarbituric acid reactive substances) assay also showed differences in the midgut. The TBARS (thiobarbituric acid reactive substances) assay also showed differences in the midgut. The results showed enzymes such as CaE3, GST, AChE, ALP, SOD, and GPX, as well as the TBARS assay, are useful biomarkers on bees. They may be used in combination as a promising tool for characterizing bee exposure to insecticides. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-15 2022-05-01T07:58:46Z 2022-05-01T07:58:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ecoenv.2021.112581 Ecotoxicology and Environmental Safety, v. 223. 1090-2414 0147-6513 http://hdl.handle.net/11449/233333 10.1016/j.ecoenv.2021.112581 2-s2.0-85111611864 |
url |
http://dx.doi.org/10.1016/j.ecoenv.2021.112581 http://hdl.handle.net/11449/233333 |
identifier_str_mv |
Ecotoxicology and Environmental Safety, v. 223. 1090-2414 0147-6513 10.1016/j.ecoenv.2021.112581 2-s2.0-85111611864 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ecotoxicology and Environmental Safety |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129474553708544 |