The Role of Bone Grafts in Preventing Medication-Related Osteonecrosis of the Jaw: Histomorphometric, Immunohistochemical, and Clinical Evaluation in Animal Model
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1177/19433875211048367 http://hdl.handle.net/11449/218701 |
Resumo: | Objective: To evaluate the effects of inorganic bovine bone graft (Lumina Bone, Criteria, Brazil) and beta-tricalcium phosphate (beta-TCP) graft (ChronOS, Synthes, Brazil) in rats with the risk of developing post-extraction medication-related osteonecrosis of the jaw (MRONJ). Methods: Eighteen male Wistar rats weighing 350 to 450 g were induced to develop MRONJ using zoledronic acid for 5 weeks. In the sixth week, the right maxillary first molar was extracted. The animals in Group I (G1) did not receive bone grafts after tooth extraction, while Group II (G2) animals received inorganic bovine bone grafts, and Group III (G3) animals received beta-tricalcium phosphate (beta-TCP) grafts. Clinical evaluation and histomorphometric and immunohistochemical analyses were performed. ANOVA and Tukey's statistical tests were used and a level of significance was considered to be 5%. Results: In the clinical evaluation, animals from G2 and G3 did not present clinical manifestations of osteonecrosis, unlike the control group (G1) animals, which presented necrotic bone tissue exposure in all samples. In the histomorphometric evaluation, animals in G3 showed greater formation of bone tissue (66%) and less formation of bone lacuna (18%) than animals in G1 (58%/32%) and in G2 (59%/27%) (P < 0.05). Moderate (++) immunostaining was observed in G2 and G3 for RANKL, TRAP, and OC, while G1 showed moderate (++) labeling for OC and mild (+) immunostaining for TRAP and RANKL. Conclusions: Greater formation of bone tissue and fewer bone lacunae were found in animals treated with beta-TCP. In clinical evaluation, bone graft groups presented with the clinical manifestation of MRONJ and showed higher intensity of immunostaining for TRAP and RANKL. Despite the limitations of experimental animal studies, the results of this work may assist in the development of future clinical research for the prevention of MRONJ. |
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The Role of Bone Grafts in Preventing Medication-Related Osteonecrosis of the Jaw: Histomorphometric, Immunohistochemical, and Clinical Evaluation in Animal Modelbisphosphonateosteonecrosisbone graftbisphosphonate-associated osteonecrosis of the jawratsWistarObjective: To evaluate the effects of inorganic bovine bone graft (Lumina Bone, Criteria, Brazil) and beta-tricalcium phosphate (beta-TCP) graft (ChronOS, Synthes, Brazil) in rats with the risk of developing post-extraction medication-related osteonecrosis of the jaw (MRONJ). Methods: Eighteen male Wistar rats weighing 350 to 450 g were induced to develop MRONJ using zoledronic acid for 5 weeks. In the sixth week, the right maxillary first molar was extracted. The animals in Group I (G1) did not receive bone grafts after tooth extraction, while Group II (G2) animals received inorganic bovine bone grafts, and Group III (G3) animals received beta-tricalcium phosphate (beta-TCP) grafts. Clinical evaluation and histomorphometric and immunohistochemical analyses were performed. ANOVA and Tukey's statistical tests were used and a level of significance was considered to be 5%. Results: In the clinical evaluation, animals from G2 and G3 did not present clinical manifestations of osteonecrosis, unlike the control group (G1) animals, which presented necrotic bone tissue exposure in all samples. In the histomorphometric evaluation, animals in G3 showed greater formation of bone tissue (66%) and less formation of bone lacuna (18%) than animals in G1 (58%/32%) and in G2 (59%/27%) (P < 0.05). Moderate (++) immunostaining was observed in G2 and G3 for RANKL, TRAP, and OC, while G1 showed moderate (++) labeling for OC and mild (+) immunostaining for TRAP and RANKL. Conclusions: Greater formation of bone tissue and fewer bone lacunae were found in animals treated with beta-TCP. In clinical evaluation, bone graft groups presented with the clinical manifestation of MRONJ and showed higher intensity of immunostaining for TRAP and RANKL. Despite the limitations of experimental animal studies, the results of this work may assist in the development of future clinical research for the prevention of MRONJ.AOCMF SwitzerlandFundacao Educ Serra Orgaos, Dept Oral & Maxillofacial Surg, Av Alberto Torres 111 Alto, BR-25964004 Teresopolis, RJ, BrazilUniv Fed Fluminense, Dept Oral & Maxillofacial Surg, Nova Friburgo, RJ, BrazilUniv Estadual Paulista, Dept Oral & Maxillofacial Surg, Aracatuba, SP, BrazilSao Paulo Estate Univ, Dept Oral & Maxillofacial Surg, Aracatuba, SP, BrazilUniv Estadual Paulista, Dept Oral & Maxillofacial Surg, Aracatuba, SP, BrazilAOCMF Switzerland: AOCMF-16-02 H.Sage Publications IncFundacao Educ Serra OrgaosUniversidade Federal Fluminense (UFF)Universidade Estadual Paulista (UNESP)Sao Paulo Estate UnivSilva, Jonathan Ribeiro daBalbas, Maria Cristina de MoraesCorrea, Caroline AguedaZanela, ManuellaOkamoto, Roberta [UNESP]Pereira, Rodrigo dos SantosHomsi, NicolasHochuli-Vieira, Eduardo [UNESP]2022-04-28T17:22:35Z2022-04-28T17:22:35Z2021-10-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8http://dx.doi.org/10.1177/19433875211048367Craniomaxillofacial Trauma & Reconstruction. Thousand Oaks: Sage Publications Inc, 8 p., 2021.1943-3875http://hdl.handle.net/11449/21870110.1177/19433875211048367WOS:000713698000001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCraniomaxillofacial Trauma & Reconstructioninfo:eu-repo/semantics/openAccess2022-04-28T17:22:35Zoai:repositorio.unesp.br:11449/218701Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:12:16.191156Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The Role of Bone Grafts in Preventing Medication-Related Osteonecrosis of the Jaw: Histomorphometric, Immunohistochemical, and Clinical Evaluation in Animal Model |
title |
The Role of Bone Grafts in Preventing Medication-Related Osteonecrosis of the Jaw: Histomorphometric, Immunohistochemical, and Clinical Evaluation in Animal Model |
spellingShingle |
The Role of Bone Grafts in Preventing Medication-Related Osteonecrosis of the Jaw: Histomorphometric, Immunohistochemical, and Clinical Evaluation in Animal Model Silva, Jonathan Ribeiro da bisphosphonate osteonecrosis bone graft bisphosphonate-associated osteonecrosis of the jaw rats Wistar |
title_short |
The Role of Bone Grafts in Preventing Medication-Related Osteonecrosis of the Jaw: Histomorphometric, Immunohistochemical, and Clinical Evaluation in Animal Model |
title_full |
The Role of Bone Grafts in Preventing Medication-Related Osteonecrosis of the Jaw: Histomorphometric, Immunohistochemical, and Clinical Evaluation in Animal Model |
title_fullStr |
The Role of Bone Grafts in Preventing Medication-Related Osteonecrosis of the Jaw: Histomorphometric, Immunohistochemical, and Clinical Evaluation in Animal Model |
title_full_unstemmed |
The Role of Bone Grafts in Preventing Medication-Related Osteonecrosis of the Jaw: Histomorphometric, Immunohistochemical, and Clinical Evaluation in Animal Model |
title_sort |
The Role of Bone Grafts in Preventing Medication-Related Osteonecrosis of the Jaw: Histomorphometric, Immunohistochemical, and Clinical Evaluation in Animal Model |
author |
Silva, Jonathan Ribeiro da |
author_facet |
Silva, Jonathan Ribeiro da Balbas, Maria Cristina de Moraes Correa, Caroline Agueda Zanela, Manuella Okamoto, Roberta [UNESP] Pereira, Rodrigo dos Santos Homsi, Nicolas Hochuli-Vieira, Eduardo [UNESP] |
author_role |
author |
author2 |
Balbas, Maria Cristina de Moraes Correa, Caroline Agueda Zanela, Manuella Okamoto, Roberta [UNESP] Pereira, Rodrigo dos Santos Homsi, Nicolas Hochuli-Vieira, Eduardo [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Fundacao Educ Serra Orgaos Universidade Federal Fluminense (UFF) Universidade Estadual Paulista (UNESP) Sao Paulo Estate Univ |
dc.contributor.author.fl_str_mv |
Silva, Jonathan Ribeiro da Balbas, Maria Cristina de Moraes Correa, Caroline Agueda Zanela, Manuella Okamoto, Roberta [UNESP] Pereira, Rodrigo dos Santos Homsi, Nicolas Hochuli-Vieira, Eduardo [UNESP] |
dc.subject.por.fl_str_mv |
bisphosphonate osteonecrosis bone graft bisphosphonate-associated osteonecrosis of the jaw rats Wistar |
topic |
bisphosphonate osteonecrosis bone graft bisphosphonate-associated osteonecrosis of the jaw rats Wistar |
description |
Objective: To evaluate the effects of inorganic bovine bone graft (Lumina Bone, Criteria, Brazil) and beta-tricalcium phosphate (beta-TCP) graft (ChronOS, Synthes, Brazil) in rats with the risk of developing post-extraction medication-related osteonecrosis of the jaw (MRONJ). Methods: Eighteen male Wistar rats weighing 350 to 450 g were induced to develop MRONJ using zoledronic acid for 5 weeks. In the sixth week, the right maxillary first molar was extracted. The animals in Group I (G1) did not receive bone grafts after tooth extraction, while Group II (G2) animals received inorganic bovine bone grafts, and Group III (G3) animals received beta-tricalcium phosphate (beta-TCP) grafts. Clinical evaluation and histomorphometric and immunohistochemical analyses were performed. ANOVA and Tukey's statistical tests were used and a level of significance was considered to be 5%. Results: In the clinical evaluation, animals from G2 and G3 did not present clinical manifestations of osteonecrosis, unlike the control group (G1) animals, which presented necrotic bone tissue exposure in all samples. In the histomorphometric evaluation, animals in G3 showed greater formation of bone tissue (66%) and less formation of bone lacuna (18%) than animals in G1 (58%/32%) and in G2 (59%/27%) (P < 0.05). Moderate (++) immunostaining was observed in G2 and G3 for RANKL, TRAP, and OC, while G1 showed moderate (++) labeling for OC and mild (+) immunostaining for TRAP and RANKL. Conclusions: Greater formation of bone tissue and fewer bone lacunae were found in animals treated with beta-TCP. In clinical evaluation, bone graft groups presented with the clinical manifestation of MRONJ and showed higher intensity of immunostaining for TRAP and RANKL. Despite the limitations of experimental animal studies, the results of this work may assist in the development of future clinical research for the prevention of MRONJ. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-30 2022-04-28T17:22:35Z 2022-04-28T17:22:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1177/19433875211048367 Craniomaxillofacial Trauma & Reconstruction. Thousand Oaks: Sage Publications Inc, 8 p., 2021. 1943-3875 http://hdl.handle.net/11449/218701 10.1177/19433875211048367 WOS:000713698000001 |
url |
http://dx.doi.org/10.1177/19433875211048367 http://hdl.handle.net/11449/218701 |
identifier_str_mv |
Craniomaxillofacial Trauma & Reconstruction. Thousand Oaks: Sage Publications Inc, 8 p., 2021. 1943-3875 10.1177/19433875211048367 WOS:000713698000001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Craniomaxillofacial Trauma & Reconstruction |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 |
dc.publisher.none.fl_str_mv |
Sage Publications Inc |
publisher.none.fl_str_mv |
Sage Publications Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128479972032512 |