Ovariectomy increases the phenotypic plasticity of the female prostate epithelium in the Mongolian gerbil (Meriones unguiculatus)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1071/RD16037 http://hdl.handle.net/11449/175049 |
Resumo: | The female prostate is a reproductive gland that typically presents a morphology similar to that of the male gland and is highly developed in female Mongolian gerbils. Two main cell populations compose the epithelium gland: basal and secretory luminal cells. However, during postnatal development, diverse secretory cell phenotypes are distributed among the typical ones. Prostate homeostasis is under the control of sexual hormones, such as oestrogen and progesterone. After hormonal deprivation the female gland undergoes several morphophysiological changes. The objective of this study was to identify and characterise, structurally and ultrastructurally, the cellular heterogeneity of the female prostate epithelium in normal conditions and after ovariectomy. Histological routine stains, such as haematoxylin-eosin, periodic acid-Schiff and silver impregnation, as well as immunocytochemical techniques were used to enable identification of the different cell types. Some secretory cells types were identified and characterised as mucinous, basophil, clear, ciliated, droplet, spumous and neuroendocrine cells. Population tally data showed that the hormonal suppression caused by ovariectomy resulted in a decrease in the proportions of basophil and clear cells and an increase in spumous cells. Thus, the secretory epithelial cells of the female gerbil prostate are not morphologically and functionally uniform, presenting a phenotypical plasticity according to the hormonal environment in which they operate. |
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Ovariectomy increases the phenotypic plasticity of the female prostate epithelium in the Mongolian gerbil (Meriones unguiculatus)cell typeselectron microscopyhormonal deprivationimmunocytochemistrysecretory cells.The female prostate is a reproductive gland that typically presents a morphology similar to that of the male gland and is highly developed in female Mongolian gerbils. Two main cell populations compose the epithelium gland: basal and secretory luminal cells. However, during postnatal development, diverse secretory cell phenotypes are distributed among the typical ones. Prostate homeostasis is under the control of sexual hormones, such as oestrogen and progesterone. After hormonal deprivation the female gland undergoes several morphophysiological changes. The objective of this study was to identify and characterise, structurally and ultrastructurally, the cellular heterogeneity of the female prostate epithelium in normal conditions and after ovariectomy. Histological routine stains, such as haematoxylin-eosin, periodic acid-Schiff and silver impregnation, as well as immunocytochemical techniques were used to enable identification of the different cell types. Some secretory cells types were identified and characterised as mucinous, basophil, clear, ciliated, droplet, spumous and neuroendocrine cells. Population tally data showed that the hormonal suppression caused by ovariectomy resulted in a decrease in the proportions of basophil and clear cells and an increase in spumous cells. Thus, the secretory epithelial cells of the female gerbil prostate are not morphologically and functionally uniform, presenting a phenotypical plasticity according to the hormonal environment in which they operate.Department of Structural and Functional Biology Institute of Biology State University of Campinas UNICAMPDepartment of Morphology Federal University of Goiás UFGLaboratory of Microscopy and Microanalysis Department of Biology São Paulo State University UNESP/IBILCE, R Cristovão Colombo, 2265Laboratory of Microscopy and Microanalysis Department of Biology São Paulo State University UNESP/IBILCE, R Cristovão Colombo, 2265Universidade Estadual de Campinas (UNICAMP)Universidade Federal de Goiás (UFG)Universidade Estadual Paulista (Unesp)Zanatelli, MariannaCustodio, Ana M. G.Biancardi, Manoel F.Santos, Fernanda C. A.Góes, Rejane M. [UNESP]Falleiros-Jr, Luiz R. [UNESP]Vilamaior, Patricia S. L. [UNESP]Taboga, Sebastião R. [UNESP]2018-12-11T17:14:00Z2018-12-11T17:14:00Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1751-1762http://dx.doi.org/10.1071/RD16037Reproduction, Fertility and Development, v. 29, n. 9, p. 1751-1762, 2017.1448-59901031-3613http://hdl.handle.net/11449/17504910.1071/RD160372-s2.0-8502751270570663581237904340000-0001-9559-5497Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproduction, Fertility and Development0,681info:eu-repo/semantics/openAccess2024-04-11T19:33:51Zoai:repositorio.unesp.br:11449/175049Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:44:24.963597Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ovariectomy increases the phenotypic plasticity of the female prostate epithelium in the Mongolian gerbil (Meriones unguiculatus) |
title |
Ovariectomy increases the phenotypic plasticity of the female prostate epithelium in the Mongolian gerbil (Meriones unguiculatus) |
spellingShingle |
Ovariectomy increases the phenotypic plasticity of the female prostate epithelium in the Mongolian gerbil (Meriones unguiculatus) Zanatelli, Marianna cell types electron microscopy hormonal deprivation immunocytochemistry secretory cells. |
title_short |
Ovariectomy increases the phenotypic plasticity of the female prostate epithelium in the Mongolian gerbil (Meriones unguiculatus) |
title_full |
Ovariectomy increases the phenotypic plasticity of the female prostate epithelium in the Mongolian gerbil (Meriones unguiculatus) |
title_fullStr |
Ovariectomy increases the phenotypic plasticity of the female prostate epithelium in the Mongolian gerbil (Meriones unguiculatus) |
title_full_unstemmed |
Ovariectomy increases the phenotypic plasticity of the female prostate epithelium in the Mongolian gerbil (Meriones unguiculatus) |
title_sort |
Ovariectomy increases the phenotypic plasticity of the female prostate epithelium in the Mongolian gerbil (Meriones unguiculatus) |
author |
Zanatelli, Marianna |
author_facet |
Zanatelli, Marianna Custodio, Ana M. G. Biancardi, Manoel F. Santos, Fernanda C. A. Góes, Rejane M. [UNESP] Falleiros-Jr, Luiz R. [UNESP] Vilamaior, Patricia S. L. [UNESP] Taboga, Sebastião R. [UNESP] |
author_role |
author |
author2 |
Custodio, Ana M. G. Biancardi, Manoel F. Santos, Fernanda C. A. Góes, Rejane M. [UNESP] Falleiros-Jr, Luiz R. [UNESP] Vilamaior, Patricia S. L. [UNESP] Taboga, Sebastião R. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Federal de Goiás (UFG) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Zanatelli, Marianna Custodio, Ana M. G. Biancardi, Manoel F. Santos, Fernanda C. A. Góes, Rejane M. [UNESP] Falleiros-Jr, Luiz R. [UNESP] Vilamaior, Patricia S. L. [UNESP] Taboga, Sebastião R. [UNESP] |
dc.subject.por.fl_str_mv |
cell types electron microscopy hormonal deprivation immunocytochemistry secretory cells. |
topic |
cell types electron microscopy hormonal deprivation immunocytochemistry secretory cells. |
description |
The female prostate is a reproductive gland that typically presents a morphology similar to that of the male gland and is highly developed in female Mongolian gerbils. Two main cell populations compose the epithelium gland: basal and secretory luminal cells. However, during postnatal development, diverse secretory cell phenotypes are distributed among the typical ones. Prostate homeostasis is under the control of sexual hormones, such as oestrogen and progesterone. After hormonal deprivation the female gland undergoes several morphophysiological changes. The objective of this study was to identify and characterise, structurally and ultrastructurally, the cellular heterogeneity of the female prostate epithelium in normal conditions and after ovariectomy. Histological routine stains, such as haematoxylin-eosin, periodic acid-Schiff and silver impregnation, as well as immunocytochemical techniques were used to enable identification of the different cell types. Some secretory cells types were identified and characterised as mucinous, basophil, clear, ciliated, droplet, spumous and neuroendocrine cells. Population tally data showed that the hormonal suppression caused by ovariectomy resulted in a decrease in the proportions of basophil and clear cells and an increase in spumous cells. Thus, the secretory epithelial cells of the female gerbil prostate are not morphologically and functionally uniform, presenting a phenotypical plasticity according to the hormonal environment in which they operate. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:14:00Z 2018-12-11T17:14:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1071/RD16037 Reproduction, Fertility and Development, v. 29, n. 9, p. 1751-1762, 2017. 1448-5990 1031-3613 http://hdl.handle.net/11449/175049 10.1071/RD16037 2-s2.0-85027512705 7066358123790434 0000-0001-9559-5497 |
url |
http://dx.doi.org/10.1071/RD16037 http://hdl.handle.net/11449/175049 |
identifier_str_mv |
Reproduction, Fertility and Development, v. 29, n. 9, p. 1751-1762, 2017. 1448-5990 1031-3613 10.1071/RD16037 2-s2.0-85027512705 7066358123790434 0000-0001-9559-5497 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproduction, Fertility and Development 0,681 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1751-1762 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129111106781184 |