Combining ultrafiltration and diffusive gradients in thin films techniques for speciation/fractionation of Cu and Zn in cytosol of liver of Nile tilapia (Oreochromis niloticus)
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/mtomcs/mfad018 http://hdl.handle.net/11449/245619 |
Resumo: | This work aims to evaluate the size and lability of Cu and Zn bound to proteins in the cytosol of fish liver of Oreochromis niloticus by employing solid-phase extraction (SPE), diffusive gradients in thin films (DGT), and ultrafiltration (UF). SPE was carried out using Chelex-100. DGT containing Chelex-100 as binding agent was employed. Analyte concentrations were determined by ICP-MS. Total Cu and Zn concentrations in cytosol (1 g of fish liver in 5 ml of Tris-HCl) ranged from 39.6 to 44.3 ng ml(-1) and 1498 to 2106 ng ml(-1), respectively. Data from UF (10-30 kDa) suggested that Cu and Zn in cytosol were associated with similar to 70% and 95%, respectively, with high-molecular-weight proteins. Cu-metallothionein was not selectively detected (although 28% of Cu was associated with low-molecular-weight proteins). However, information about the specific proteins in the cytosol will require coupling UF with organic mass spectrometry. Data from SPE showed the presence of labile Cu species of similar to 17%, while the fraction of labile Zn species was >55%. However, data from DGT suggested a fraction of labile Cu species only of 7% and a labile Zn fraction of 5%. This data, as compared with previous data from literature, suggests that the DGT technique gave a more plausible estimation of the labile pool of Zn and Cu in cytosol. The combination of results from UF and DGT is capable of contributing to the knowledge about the labile and low-molecular pool of Cu and Zn. |
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Repositório Institucional da UNESP |
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Combining ultrafiltration and diffusive gradients in thin films techniques for speciation/fractionation of Cu and Zn in cytosol of liver of Nile tilapia (Oreochromis niloticus)cytosolfish livermetalsdiffusive gradient in thin filmssolid phase extractionultrafiltrationThis work aims to evaluate the size and lability of Cu and Zn bound to proteins in the cytosol of fish liver of Oreochromis niloticus by employing solid-phase extraction (SPE), diffusive gradients in thin films (DGT), and ultrafiltration (UF). SPE was carried out using Chelex-100. DGT containing Chelex-100 as binding agent was employed. Analyte concentrations were determined by ICP-MS. Total Cu and Zn concentrations in cytosol (1 g of fish liver in 5 ml of Tris-HCl) ranged from 39.6 to 44.3 ng ml(-1) and 1498 to 2106 ng ml(-1), respectively. Data from UF (10-30 kDa) suggested that Cu and Zn in cytosol were associated with similar to 70% and 95%, respectively, with high-molecular-weight proteins. Cu-metallothionein was not selectively detected (although 28% of Cu was associated with low-molecular-weight proteins). However, information about the specific proteins in the cytosol will require coupling UF with organic mass spectrometry. Data from SPE showed the presence of labile Cu species of similar to 17%, while the fraction of labile Zn species was >55%. However, data from DGT suggested a fraction of labile Cu species only of 7% and a labile Zn fraction of 5%. This data, as compared with previous data from literature, suggests that the DGT technique gave a more plausible estimation of the labile pool of Zn and Cu in cytosol. The combination of results from UF and DGT is capable of contributing to the knowledge about the labile and low-molecular pool of Cu and Zn.Funda��o de Amparo � Pesquisa do Estado de S�o Paulo (FAPESP)Coordena��o de Aperfei�oamento de Pessoal de N�vel Superior (CAPES)Conselho Nacional de Desenvolvimento Cient�fico e Tecnol�gico (CNPq)PetrobrasFunda��o para o Desenvolvimento da UNESP (FUNDUNESP)Sao Paulo State Univ UNESP, Environm Studies Ctr CEA, Ave 24 A,1515, BR-13506900 Rio Claro, SP, BrazilSao Paulo State Univ UNESP, Inst Geosci & Exact Sci, Dept Geol Basin Studies Lab LEBAC, Ave 24 A,1515, BR-13506900 Rio Claro, SP, BrazilSao Paulo State Univ UNESP, Dept Geog & Environm Planning, Ave 24 A,1515, BR-13506900 Rio Claro, SP, BrazilSao Paulo State Univ UNESP, Sci & Technol Inst, Dept Environm Engn, Km 137,8, BR-12247004 Sao Jose Dos Campos, SP, BrazilSao Paulo State Univ UNESP, Environm Studies Ctr CEA, Ave 24 A,1515, BR-13506900 Rio Claro, SP, BrazilSao Paulo State Univ UNESP, Inst Geosci & Exact Sci, Dept Geol Basin Studies Lab LEBAC, Ave 24 A,1515, BR-13506900 Rio Claro, SP, BrazilSao Paulo State Univ UNESP, Dept Geog & Environm Planning, Ave 24 A,1515, BR-13506900 Rio Claro, SP, BrazilSao Paulo State Univ UNESP, Sci & Technol Inst, Dept Environm Engn, Km 137,8, BR-12247004 Sao Jose Dos Campos, SP, BrazilFAPESP: 2021/00572-0CNPq: 308090/2019-5FUNDUNESP: 5900.0111820.19.9-Item 35Oxford Univ PressUniversidade Estadual Paulista (UNESP)Gemeiner, Hendryk [UNESP]Menegario, Amauri Antonio [UNESP]Eismann, Carlos Eduardo [UNESP]Elias, Lucas Pellegrini [UNESP]Pedrobom, Jorge Henrique [UNESP]Dourado, Thiago de Araujo [UNESP]Chang, Hung Kiang [UNESP]Conceicao, Fabiano Tomazini da [UNESP]Moruzzi, Rodrigo Braga [UNESP]2023-07-29T12:00:11Z2023-07-29T12:00:11Z2023-04-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8http://dx.doi.org/10.1093/mtomcs/mfad018Metallomics. Oxford: Oxford Univ Press, v. 15, n. 4, 8 p., 2023.1756-5901http://hdl.handle.net/11449/24561910.1093/mtomcs/mfad018WOS:000969807500001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMetallomicsinfo:eu-repo/semantics/openAccess2023-07-29T12:00:11Zoai:repositorio.unesp.br:11449/245619Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:28:04.135061Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Combining ultrafiltration and diffusive gradients in thin films techniques for speciation/fractionation of Cu and Zn in cytosol of liver of Nile tilapia (Oreochromis niloticus) |
title |
Combining ultrafiltration and diffusive gradients in thin films techniques for speciation/fractionation of Cu and Zn in cytosol of liver of Nile tilapia (Oreochromis niloticus) |
spellingShingle |
Combining ultrafiltration and diffusive gradients in thin films techniques for speciation/fractionation of Cu and Zn in cytosol of liver of Nile tilapia (Oreochromis niloticus) Gemeiner, Hendryk [UNESP] cytosol fish liver metals diffusive gradient in thin films solid phase extraction ultrafiltration |
title_short |
Combining ultrafiltration and diffusive gradients in thin films techniques for speciation/fractionation of Cu and Zn in cytosol of liver of Nile tilapia (Oreochromis niloticus) |
title_full |
Combining ultrafiltration and diffusive gradients in thin films techniques for speciation/fractionation of Cu and Zn in cytosol of liver of Nile tilapia (Oreochromis niloticus) |
title_fullStr |
Combining ultrafiltration and diffusive gradients in thin films techniques for speciation/fractionation of Cu and Zn in cytosol of liver of Nile tilapia (Oreochromis niloticus) |
title_full_unstemmed |
Combining ultrafiltration and diffusive gradients in thin films techniques for speciation/fractionation of Cu and Zn in cytosol of liver of Nile tilapia (Oreochromis niloticus) |
title_sort |
Combining ultrafiltration and diffusive gradients in thin films techniques for speciation/fractionation of Cu and Zn in cytosol of liver of Nile tilapia (Oreochromis niloticus) |
author |
Gemeiner, Hendryk [UNESP] |
author_facet |
Gemeiner, Hendryk [UNESP] Menegario, Amauri Antonio [UNESP] Eismann, Carlos Eduardo [UNESP] Elias, Lucas Pellegrini [UNESP] Pedrobom, Jorge Henrique [UNESP] Dourado, Thiago de Araujo [UNESP] Chang, Hung Kiang [UNESP] Conceicao, Fabiano Tomazini da [UNESP] Moruzzi, Rodrigo Braga [UNESP] |
author_role |
author |
author2 |
Menegario, Amauri Antonio [UNESP] Eismann, Carlos Eduardo [UNESP] Elias, Lucas Pellegrini [UNESP] Pedrobom, Jorge Henrique [UNESP] Dourado, Thiago de Araujo [UNESP] Chang, Hung Kiang [UNESP] Conceicao, Fabiano Tomazini da [UNESP] Moruzzi, Rodrigo Braga [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Gemeiner, Hendryk [UNESP] Menegario, Amauri Antonio [UNESP] Eismann, Carlos Eduardo [UNESP] Elias, Lucas Pellegrini [UNESP] Pedrobom, Jorge Henrique [UNESP] Dourado, Thiago de Araujo [UNESP] Chang, Hung Kiang [UNESP] Conceicao, Fabiano Tomazini da [UNESP] Moruzzi, Rodrigo Braga [UNESP] |
dc.subject.por.fl_str_mv |
cytosol fish liver metals diffusive gradient in thin films solid phase extraction ultrafiltration |
topic |
cytosol fish liver metals diffusive gradient in thin films solid phase extraction ultrafiltration |
description |
This work aims to evaluate the size and lability of Cu and Zn bound to proteins in the cytosol of fish liver of Oreochromis niloticus by employing solid-phase extraction (SPE), diffusive gradients in thin films (DGT), and ultrafiltration (UF). SPE was carried out using Chelex-100. DGT containing Chelex-100 as binding agent was employed. Analyte concentrations were determined by ICP-MS. Total Cu and Zn concentrations in cytosol (1 g of fish liver in 5 ml of Tris-HCl) ranged from 39.6 to 44.3 ng ml(-1) and 1498 to 2106 ng ml(-1), respectively. Data from UF (10-30 kDa) suggested that Cu and Zn in cytosol were associated with similar to 70% and 95%, respectively, with high-molecular-weight proteins. Cu-metallothionein was not selectively detected (although 28% of Cu was associated with low-molecular-weight proteins). However, information about the specific proteins in the cytosol will require coupling UF with organic mass spectrometry. Data from SPE showed the presence of labile Cu species of similar to 17%, while the fraction of labile Zn species was >55%. However, data from DGT suggested a fraction of labile Cu species only of 7% and a labile Zn fraction of 5%. This data, as compared with previous data from literature, suggests that the DGT technique gave a more plausible estimation of the labile pool of Zn and Cu in cytosol. The combination of results from UF and DGT is capable of contributing to the knowledge about the labile and low-molecular pool of Cu and Zn. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:00:11Z 2023-07-29T12:00:11Z 2023-04-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/mtomcs/mfad018 Metallomics. Oxford: Oxford Univ Press, v. 15, n. 4, 8 p., 2023. 1756-5901 http://hdl.handle.net/11449/245619 10.1093/mtomcs/mfad018 WOS:000969807500001 |
url |
http://dx.doi.org/10.1093/mtomcs/mfad018 http://hdl.handle.net/11449/245619 |
identifier_str_mv |
Metallomics. Oxford: Oxford Univ Press, v. 15, n. 4, 8 p., 2023. 1756-5901 10.1093/mtomcs/mfad018 WOS:000969807500001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Metallomics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 |
dc.publisher.none.fl_str_mv |
Oxford Univ Press |
publisher.none.fl_str_mv |
Oxford Univ Press |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128657535795200 |