Impact of resistance to fluconazole on virulence and morphological aspects of Cryptococcus neoformans and Cryptococcus gattii isolates
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fmicb.2016.00153 http://hdl.handle.net/11449/172755 |
Resumo: | Cryptococcus sp. are responsible for around 1 million cases of meningitis every year. Fluconazole (FLU) is commonly used in the treatment of cryptococcosis, mainly in immunocompromised patients and the resistance is usually reported after long periods of treatment. In this study, the morphological characterization and virulence profile of FLU-susceptible and FLU-resistant clinical and environmental isolates of C. neoformans and C. gattii were performed both in vitro and in vivo using the Galleria mellonella model. FLU-susceptible isolates from C. neoformans were significantly more virulent than the FLU-resistant isolates. FLU-susceptible C. gattii isolates showed a different virulence profile from C. neoformans isolates where only the environmental isolate, CL, was more virulent compared with the resistant isolates. Cell morphology and capsule size were analyzed and the FLU-resistant isolates did not change significantly compared with the most sensitive isolates. Growth at 37°C was also evaluated and in both species, the resistant isolates showed a reduced growth at this temperature, indicating that FLU resistance can affect their growth. Based on the results obtained is possible suggest that FLU resistance can influence the morphology of the isolates and consequently changed the virulence profiles. The most evident results were observed for C. neoformans showing that the adaptation of isolates to antifungal selective pressure influenced the loss of virulence. |
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Impact of resistance to fluconazole on virulence and morphological aspects of Cryptococcus neoformans and Cryptococcus gattii isolatesCryptococcus sp.FluconazoleGalleria mellonellaResistanceVirulenceCryptococcus sp. are responsible for around 1 million cases of meningitis every year. Fluconazole (FLU) is commonly used in the treatment of cryptococcosis, mainly in immunocompromised patients and the resistance is usually reported after long periods of treatment. In this study, the morphological characterization and virulence profile of FLU-susceptible and FLU-resistant clinical and environmental isolates of C. neoformans and C. gattii were performed both in vitro and in vivo using the Galleria mellonella model. FLU-susceptible isolates from C. neoformans were significantly more virulent than the FLU-resistant isolates. FLU-susceptible C. gattii isolates showed a different virulence profile from C. neoformans isolates where only the environmental isolate, CL, was more virulent compared with the resistant isolates. Cell morphology and capsule size were analyzed and the FLU-resistant isolates did not change significantly compared with the most sensitive isolates. Growth at 37°C was also evaluated and in both species, the resistant isolates showed a reduced growth at this temperature, indicating that FLU resistance can affect their growth. Based on the results obtained is possible suggest that FLU resistance can influence the morphology of the isolates and consequently changed the virulence profiles. The most evident results were observed for C. neoformans showing that the adaptation of isolates to antifungal selective pressure influenced the loss of virulence.Faculdade de Ciências Farmacêuticas UNESP - Universidade Estadual Paulista Campus Araraquara Departamento de Análises ClíncasCentro Nacional de Microbiologia Unidad de Micologia Instituto de Salud Carlos IIIDermatology Research Group Universidad de AntioquiaFaculdade de Medicina Veterinária e Zootecnia USP - Universidade de São Paulo Departamento de PatologiaFaculdade de Ciências Farmacêuticas UNESP - Universidade Estadual Paulista Campus Araraquara Departamento de Análises ClíncasUniversidade Estadual Paulista (Unesp)Instituto de Salud Carlos IIIUniversidad de AntioquiaUniversidade de São Paulo (USP)Rossi, Suélen A. [UNESP]Trevijano-Contador, NuriaScorzoni, Liliana [UNESP]Mesa-Arango, Ana CeciliaDe Oliveira, Haroldo C. [UNESP]Werther, KarinDe Freitas Raso, TâniaMendes-Giannini, Maria J.S. [UNESP]Zaragoza, OscarFusco-Almeida, Ana M. [UNESP]2018-12-11T17:02:03Z2018-12-11T17:02:03Z2016-02-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.3389/fmicb.2016.00153Frontiers in Microbiology, v. 7, n. FEB, 2016.1664-302Xhttp://hdl.handle.net/11449/17275510.3389/fmicb.2016.001532-s2.0-849621796302-s2.0-84962179630.pdf88468034995628190000-0002-8822-488XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Microbiologyinfo:eu-repo/semantics/openAccess2024-06-21T15:18:45Zoai:repositorio.unesp.br:11449/172755Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:18:27.865496Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Impact of resistance to fluconazole on virulence and morphological aspects of Cryptococcus neoformans and Cryptococcus gattii isolates |
title |
Impact of resistance to fluconazole on virulence and morphological aspects of Cryptococcus neoformans and Cryptococcus gattii isolates |
spellingShingle |
Impact of resistance to fluconazole on virulence and morphological aspects of Cryptococcus neoformans and Cryptococcus gattii isolates Rossi, Suélen A. [UNESP] Cryptococcus sp. Fluconazole Galleria mellonella Resistance Virulence |
title_short |
Impact of resistance to fluconazole on virulence and morphological aspects of Cryptococcus neoformans and Cryptococcus gattii isolates |
title_full |
Impact of resistance to fluconazole on virulence and morphological aspects of Cryptococcus neoformans and Cryptococcus gattii isolates |
title_fullStr |
Impact of resistance to fluconazole on virulence and morphological aspects of Cryptococcus neoformans and Cryptococcus gattii isolates |
title_full_unstemmed |
Impact of resistance to fluconazole on virulence and morphological aspects of Cryptococcus neoformans and Cryptococcus gattii isolates |
title_sort |
Impact of resistance to fluconazole on virulence and morphological aspects of Cryptococcus neoformans and Cryptococcus gattii isolates |
author |
Rossi, Suélen A. [UNESP] |
author_facet |
Rossi, Suélen A. [UNESP] Trevijano-Contador, Nuria Scorzoni, Liliana [UNESP] Mesa-Arango, Ana Cecilia De Oliveira, Haroldo C. [UNESP] Werther, Karin De Freitas Raso, Tânia Mendes-Giannini, Maria J.S. [UNESP] Zaragoza, Oscar Fusco-Almeida, Ana M. [UNESP] |
author_role |
author |
author2 |
Trevijano-Contador, Nuria Scorzoni, Liliana [UNESP] Mesa-Arango, Ana Cecilia De Oliveira, Haroldo C. [UNESP] Werther, Karin De Freitas Raso, Tânia Mendes-Giannini, Maria J.S. [UNESP] Zaragoza, Oscar Fusco-Almeida, Ana M. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Instituto de Salud Carlos III Universidad de Antioquia Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Rossi, Suélen A. [UNESP] Trevijano-Contador, Nuria Scorzoni, Liliana [UNESP] Mesa-Arango, Ana Cecilia De Oliveira, Haroldo C. [UNESP] Werther, Karin De Freitas Raso, Tânia Mendes-Giannini, Maria J.S. [UNESP] Zaragoza, Oscar Fusco-Almeida, Ana M. [UNESP] |
dc.subject.por.fl_str_mv |
Cryptococcus sp. Fluconazole Galleria mellonella Resistance Virulence |
topic |
Cryptococcus sp. Fluconazole Galleria mellonella Resistance Virulence |
description |
Cryptococcus sp. are responsible for around 1 million cases of meningitis every year. Fluconazole (FLU) is commonly used in the treatment of cryptococcosis, mainly in immunocompromised patients and the resistance is usually reported after long periods of treatment. In this study, the morphological characterization and virulence profile of FLU-susceptible and FLU-resistant clinical and environmental isolates of C. neoformans and C. gattii were performed both in vitro and in vivo using the Galleria mellonella model. FLU-susceptible isolates from C. neoformans were significantly more virulent than the FLU-resistant isolates. FLU-susceptible C. gattii isolates showed a different virulence profile from C. neoformans isolates where only the environmental isolate, CL, was more virulent compared with the resistant isolates. Cell morphology and capsule size were analyzed and the FLU-resistant isolates did not change significantly compared with the most sensitive isolates. Growth at 37°C was also evaluated and in both species, the resistant isolates showed a reduced growth at this temperature, indicating that FLU resistance can affect their growth. Based on the results obtained is possible suggest that FLU resistance can influence the morphology of the isolates and consequently changed the virulence profiles. The most evident results were observed for C. neoformans showing that the adaptation of isolates to antifungal selective pressure influenced the loss of virulence. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-16 2018-12-11T17:02:03Z 2018-12-11T17:02:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fmicb.2016.00153 Frontiers in Microbiology, v. 7, n. FEB, 2016. 1664-302X http://hdl.handle.net/11449/172755 10.3389/fmicb.2016.00153 2-s2.0-84962179630 2-s2.0-84962179630.pdf 8846803499562819 0000-0002-8822-488X |
url |
http://dx.doi.org/10.3389/fmicb.2016.00153 http://hdl.handle.net/11449/172755 |
identifier_str_mv |
Frontiers in Microbiology, v. 7, n. FEB, 2016. 1664-302X 10.3389/fmicb.2016.00153 2-s2.0-84962179630 2-s2.0-84962179630.pdf 8846803499562819 0000-0002-8822-488X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128789394227200 |