Vitamin D decreases expression of NLRP1 and NLRP3 inflammasomes in placental explants from women with preeclampsia cultured with hydrogen peroxide
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.humimm.2021.10.002 http://hdl.handle.net/11449/233736 |
Resumo: | This study aimed to evaluate the immunomodulatory effect of vitamin D (VD) on the NLRP1 and NLRP3 inflammasomes in placental explants from preeclamptic (PE) and normotensive (NT) pregnant women. Placental explants from eight PE and eight NT pregnant women were cultured with or without hydrogen peroxide (H2O2), VD or H2O2 + VD. Gene and protein expression of NLRP1, NLRP3, HMGB1, caspase-1, IL-1β, TNF-α and IL-18 were determined by qPCR and Western blotting/ELISA. Compared to NT pregnant women, the endogenous gene expression of NLRP1, NLRP3, HMGB1, IL-1β, TNF-α and IL-18 was significantly higher in explants from PE and became decreased after VD treatment. Similarly, VD decreased the protein expression of NLRP1, NLRP3, caspase-1, HMGB1, IL-1β, TNF-α and IL-18 in PE. Placental explants from NT cultured with H2O2 showed increased gene and protein expression of NLRP1, NLRP3, caspase-1, IL-1β, TNF-α and HMGB1, while H2O2 was also able to increase TNF-α and caspase-1 gene expression in PE. Treatment with H2O2 + VD decreased gene/protein expression of NLRP1, NLRP3, caspase-1, HMGB1, IL-1β, TNF-α and IL-18 in PE and NT explants with H2O2. NLRP1 and NLRP3 are upregulated in the PE. VD may play an immunomodulatory role in the placental inflammation and downregulates oxidative stress induced in vitro by H2O2. |
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Vitamin D decreases expression of NLRP1 and NLRP3 inflammasomes in placental explants from women with preeclampsia cultured with hydrogen peroxideCytokinesInflammasomePlacental explantsPreeclampsiaVitamin DThis study aimed to evaluate the immunomodulatory effect of vitamin D (VD) on the NLRP1 and NLRP3 inflammasomes in placental explants from preeclamptic (PE) and normotensive (NT) pregnant women. Placental explants from eight PE and eight NT pregnant women were cultured with or without hydrogen peroxide (H2O2), VD or H2O2 + VD. Gene and protein expression of NLRP1, NLRP3, HMGB1, caspase-1, IL-1β, TNF-α and IL-18 were determined by qPCR and Western blotting/ELISA. Compared to NT pregnant women, the endogenous gene expression of NLRP1, NLRP3, HMGB1, IL-1β, TNF-α and IL-18 was significantly higher in explants from PE and became decreased after VD treatment. Similarly, VD decreased the protein expression of NLRP1, NLRP3, caspase-1, HMGB1, IL-1β, TNF-α and IL-18 in PE. Placental explants from NT cultured with H2O2 showed increased gene and protein expression of NLRP1, NLRP3, caspase-1, IL-1β, TNF-α and HMGB1, while H2O2 was also able to increase TNF-α and caspase-1 gene expression in PE. Treatment with H2O2 + VD decreased gene/protein expression of NLRP1, NLRP3, caspase-1, HMGB1, IL-1β, TNF-α and IL-18 in PE and NT explants with H2O2. NLRP1 and NLRP3 are upregulated in the PE. VD may play an immunomodulatory role in the placental inflammation and downregulates oxidative stress induced in vitro by H2O2.Botucatu Medical School Sao Paulo State University (Unesp)Institute of Biosciences Sao Paulo State University (Unesp)Botucatu Medical School Sao Paulo State University (Unesp)Institute of Biosciences Sao Paulo State University (Unesp)Universidade Estadual Paulista (UNESP)Nunes, Priscila R. [UNESP]Romao-Veiga, Mariana [UNESP]Matias, Mariana L. [UNESP]Ribeiro, Vanessa R. [UNESP]de Oliveira, Leandro [UNESP]Peracoli, Jose Carlos [UNESP]Terezinha S. Peracoli, Maria [UNESP]2022-05-01T09:47:28Z2022-05-01T09:47:28Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article74-80http://dx.doi.org/10.1016/j.humimm.2021.10.002Human Immunology, v. 83, n. 1, p. 74-80, 2022.1879-11660198-8859http://hdl.handle.net/11449/23373610.1016/j.humimm.2021.10.0022-s2.0-85117768866Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHuman Immunologyinfo:eu-repo/semantics/openAccess2024-08-16T14:13:02Zoai:repositorio.unesp.br:11449/233736Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:13:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Vitamin D decreases expression of NLRP1 and NLRP3 inflammasomes in placental explants from women with preeclampsia cultured with hydrogen peroxide |
title |
Vitamin D decreases expression of NLRP1 and NLRP3 inflammasomes in placental explants from women with preeclampsia cultured with hydrogen peroxide |
spellingShingle |
Vitamin D decreases expression of NLRP1 and NLRP3 inflammasomes in placental explants from women with preeclampsia cultured with hydrogen peroxide Nunes, Priscila R. [UNESP] Cytokines Inflammasome Placental explants Preeclampsia Vitamin D |
title_short |
Vitamin D decreases expression of NLRP1 and NLRP3 inflammasomes in placental explants from women with preeclampsia cultured with hydrogen peroxide |
title_full |
Vitamin D decreases expression of NLRP1 and NLRP3 inflammasomes in placental explants from women with preeclampsia cultured with hydrogen peroxide |
title_fullStr |
Vitamin D decreases expression of NLRP1 and NLRP3 inflammasomes in placental explants from women with preeclampsia cultured with hydrogen peroxide |
title_full_unstemmed |
Vitamin D decreases expression of NLRP1 and NLRP3 inflammasomes in placental explants from women with preeclampsia cultured with hydrogen peroxide |
title_sort |
Vitamin D decreases expression of NLRP1 and NLRP3 inflammasomes in placental explants from women with preeclampsia cultured with hydrogen peroxide |
author |
Nunes, Priscila R. [UNESP] |
author_facet |
Nunes, Priscila R. [UNESP] Romao-Veiga, Mariana [UNESP] Matias, Mariana L. [UNESP] Ribeiro, Vanessa R. [UNESP] de Oliveira, Leandro [UNESP] Peracoli, Jose Carlos [UNESP] Terezinha S. Peracoli, Maria [UNESP] |
author_role |
author |
author2 |
Romao-Veiga, Mariana [UNESP] Matias, Mariana L. [UNESP] Ribeiro, Vanessa R. [UNESP] de Oliveira, Leandro [UNESP] Peracoli, Jose Carlos [UNESP] Terezinha S. Peracoli, Maria [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Nunes, Priscila R. [UNESP] Romao-Veiga, Mariana [UNESP] Matias, Mariana L. [UNESP] Ribeiro, Vanessa R. [UNESP] de Oliveira, Leandro [UNESP] Peracoli, Jose Carlos [UNESP] Terezinha S. Peracoli, Maria [UNESP] |
dc.subject.por.fl_str_mv |
Cytokines Inflammasome Placental explants Preeclampsia Vitamin D |
topic |
Cytokines Inflammasome Placental explants Preeclampsia Vitamin D |
description |
This study aimed to evaluate the immunomodulatory effect of vitamin D (VD) on the NLRP1 and NLRP3 inflammasomes in placental explants from preeclamptic (PE) and normotensive (NT) pregnant women. Placental explants from eight PE and eight NT pregnant women were cultured with or without hydrogen peroxide (H2O2), VD or H2O2 + VD. Gene and protein expression of NLRP1, NLRP3, HMGB1, caspase-1, IL-1β, TNF-α and IL-18 were determined by qPCR and Western blotting/ELISA. Compared to NT pregnant women, the endogenous gene expression of NLRP1, NLRP3, HMGB1, IL-1β, TNF-α and IL-18 was significantly higher in explants from PE and became decreased after VD treatment. Similarly, VD decreased the protein expression of NLRP1, NLRP3, caspase-1, HMGB1, IL-1β, TNF-α and IL-18 in PE. Placental explants from NT cultured with H2O2 showed increased gene and protein expression of NLRP1, NLRP3, caspase-1, IL-1β, TNF-α and HMGB1, while H2O2 was also able to increase TNF-α and caspase-1 gene expression in PE. Treatment with H2O2 + VD decreased gene/protein expression of NLRP1, NLRP3, caspase-1, HMGB1, IL-1β, TNF-α and IL-18 in PE and NT explants with H2O2. NLRP1 and NLRP3 are upregulated in the PE. VD may play an immunomodulatory role in the placental inflammation and downregulates oxidative stress induced in vitro by H2O2. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01T09:47:28Z 2022-05-01T09:47:28Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.humimm.2021.10.002 Human Immunology, v. 83, n. 1, p. 74-80, 2022. 1879-1166 0198-8859 http://hdl.handle.net/11449/233736 10.1016/j.humimm.2021.10.002 2-s2.0-85117768866 |
url |
http://dx.doi.org/10.1016/j.humimm.2021.10.002 http://hdl.handle.net/11449/233736 |
identifier_str_mv |
Human Immunology, v. 83, n. 1, p. 74-80, 2022. 1879-1166 0198-8859 10.1016/j.humimm.2021.10.002 2-s2.0-85117768866 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Human Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
74-80 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128213379973120 |