In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/219653 |
Resumo: | Objective: We investigated the in vitro and skin lesions production of cytokines in non-treated borderline tuberculoid (BT) and borderline lepromatous (BL) patients. Patients and Methods: Seven untreated, non-reactional BT patients and 12 untreated, non-reactional BL patients were studied. Levels of the cytokines IFN-γ, IL-10, TGF-β1 and TNF-α were measured in supernantant of peripheral blood mononuclear cells (PBMC) cultures, stimulated with specific M. leprae antigen (sonicated and whole). The cytokines iNOS, IL-10 and TGF-β1 were detected by immunohistochemistry in skin biopsies. Results: BT patients produced higher levels of IFN-γ than BL patients; iNOS expression in skin lesions was also higher in BT patients. TGF-β1 was detected in more cells in BL patients; IL-10 expression was similar in both groups. There was a negative correlation between iNOS and TGF-β1 expression in skin biopsies, positive correlation between TGF-b1 in skin lesions and bacillary index, as well as positive correlation between iNOS detected in skin biopsies and PBMC IFN- γ production. Conclusions: The BT patients had a mainly a Th1-profile of cytokines in their skin lesions and BL patients had aTh2 profile. © Lepra. |
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Repositório Institucional da UNESP |
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In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosyObjective: We investigated the in vitro and skin lesions production of cytokines in non-treated borderline tuberculoid (BT) and borderline lepromatous (BL) patients. Patients and Methods: Seven untreated, non-reactional BT patients and 12 untreated, non-reactional BL patients were studied. Levels of the cytokines IFN-γ, IL-10, TGF-β1 and TNF-α were measured in supernantant of peripheral blood mononuclear cells (PBMC) cultures, stimulated with specific M. leprae antigen (sonicated and whole). The cytokines iNOS, IL-10 and TGF-β1 were detected by immunohistochemistry in skin biopsies. Results: BT patients produced higher levels of IFN-γ than BL patients; iNOS expression in skin lesions was also higher in BT patients. TGF-β1 was detected in more cells in BL patients; IL-10 expression was similar in both groups. There was a negative correlation between iNOS and TGF-β1 expression in skin biopsies, positive correlation between TGF-b1 in skin lesions and bacillary index, as well as positive correlation between iNOS detected in skin biopsies and PBMC IFN- γ production. Conclusions: The BT patients had a mainly a Th1-profile of cytokines in their skin lesions and BL patients had aTh2 profile. © Lepra.Faculdade de Medicina de Botucatu UNESP, Botucatu, SPEquipe Técnica de Patologia Instituto Lauro de Souza Lima, Bauru, SPServiço de Epidemiologia Instituto Lauro de Souza Lima, Bauru, SPDivisão de Pesquisa e Ensino Instituto Lauro de Souza Lima, Bauru, SPFaculdade de Odontologia de Bauru Universidade de São Paulo, Bauru, SPFaculdade de Medicina de Botucatu UNESP, Botucatu, SPUniversidade Estadual Paulista (UNESP)Instituto Lauro de Souza LimaUniversidade de São Paulo (USP)Venturini, James [UNESP]Soares, Cléverson TeixeiraBelone, Andréa de Faria FernandesBarreto, Jaison AntonioUra, SomeiLauris, José Roberto PereiraVilani-Moreno, Fátima Regina2022-04-28T18:56:47Z2022-04-28T18:56:47Z2011-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article25-35Leprosy Review, v. 82, n. 1, p. 25-35, 2011.0305-7518http://hdl.handle.net/11449/2196532-s2.0-79957466881Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLeprosy Reviewinfo:eu-repo/semantics/openAccess2022-04-28T18:56:48Zoai:repositorio.unesp.br:11449/219653Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:42:48.133453Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy |
title |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy |
spellingShingle |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy Venturini, James [UNESP] |
title_short |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy |
title_full |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy |
title_fullStr |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy |
title_full_unstemmed |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy |
title_sort |
In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy |
author |
Venturini, James [UNESP] |
author_facet |
Venturini, James [UNESP] Soares, Cléverson Teixeira Belone, Andréa de Faria Fernandes Barreto, Jaison Antonio Ura, Somei Lauris, José Roberto Pereira Vilani-Moreno, Fátima Regina |
author_role |
author |
author2 |
Soares, Cléverson Teixeira Belone, Andréa de Faria Fernandes Barreto, Jaison Antonio Ura, Somei Lauris, José Roberto Pereira Vilani-Moreno, Fátima Regina |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Instituto Lauro de Souza Lima Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Venturini, James [UNESP] Soares, Cléverson Teixeira Belone, Andréa de Faria Fernandes Barreto, Jaison Antonio Ura, Somei Lauris, José Roberto Pereira Vilani-Moreno, Fátima Regina |
description |
Objective: We investigated the in vitro and skin lesions production of cytokines in non-treated borderline tuberculoid (BT) and borderline lepromatous (BL) patients. Patients and Methods: Seven untreated, non-reactional BT patients and 12 untreated, non-reactional BL patients were studied. Levels of the cytokines IFN-γ, IL-10, TGF-β1 and TNF-α were measured in supernantant of peripheral blood mononuclear cells (PBMC) cultures, stimulated with specific M. leprae antigen (sonicated and whole). The cytokines iNOS, IL-10 and TGF-β1 were detected by immunohistochemistry in skin biopsies. Results: BT patients produced higher levels of IFN-γ than BL patients; iNOS expression in skin lesions was also higher in BT patients. TGF-β1 was detected in more cells in BL patients; IL-10 expression was similar in both groups. There was a negative correlation between iNOS and TGF-β1 expression in skin biopsies, positive correlation between TGF-b1 in skin lesions and bacillary index, as well as positive correlation between iNOS detected in skin biopsies and PBMC IFN- γ production. Conclusions: The BT patients had a mainly a Th1-profile of cytokines in their skin lesions and BL patients had aTh2 profile. © Lepra. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-01 2022-04-28T18:56:47Z 2022-04-28T18:56:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Leprosy Review, v. 82, n. 1, p. 25-35, 2011. 0305-7518 http://hdl.handle.net/11449/219653 2-s2.0-79957466881 |
identifier_str_mv |
Leprosy Review, v. 82, n. 1, p. 25-35, 2011. 0305-7518 2-s2.0-79957466881 |
url |
http://hdl.handle.net/11449/219653 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Leprosy Review |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
25-35 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128848709025792 |