Chromosomal Mapping of Repetitive DNAs in Characidium (Teleostei, Characiformes): Genomic Organization and Diversification of ZW Sex Chromosomes
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1159/000437165 http://hdl.handle.net/11449/160865 |
Resumo: | The speciose neotropical genus Characidium has proven to be a good model for cytogenetic exploration. Representatives of this genus often have a conserved diploid chromosome number; some species exhibit a highly differentiated ZZ/ZW sex chromosome system, while others do not show any sex-related chromosome heteromorphism. In this study, chromosome painting using a W-specific probe and comparative chromosome mapping of repetitive sequences, including ribosomal clusters and 4 microsatellite motifs - (CA)(15), (GA)(15), (CG)(15), and (TTA)(10) -, were performed in 6 Characidium species, 5 of which possessed a heteromorphic ZW sex chromosome system. The W-specific probe showed hybridization signals on the W chromosome of all analyzed species, indicating homology among the W chromosomes. Remarkably, a single major rDNA-bearing chromosome pair was found in all species. The 18S rDNA localized to the sex chromosomes in C. lanei, C. timbuiense and C. pterostictum, while the major rDNA localized to one autosome pair in C. vidali and C. gomesi. In contrast, the number of 5S rDNA-bearing chromosomes varied. Notably, minor ribosomal clusters were identified in the W chromosome of C. vidali. Microsatellites were widely distributed across almost all chromosomes of the karyotypes, with a greater accumulation in the subtelomeric regions. However, clear differences in the abundance of each motif were detected in each species. In addition, the Z and W chromosomes showed the differential accumulation of distinct motifs. Our results revealed variability in the distribution of repetitive DNA sequences and their possible association with sex chromosome diversification in Characidium species. (C) 2015 S. Karger AG, Basel |
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Chromosomal Mapping of Repetitive DNAs in Characidium (Teleostei, Characiformes): Genomic Organization and Diversification of ZW Sex ChromosomesChromosome paintingCrenuchidaeFish cytogeneticsKaryotype diversificationThe speciose neotropical genus Characidium has proven to be a good model for cytogenetic exploration. Representatives of this genus often have a conserved diploid chromosome number; some species exhibit a highly differentiated ZZ/ZW sex chromosome system, while others do not show any sex-related chromosome heteromorphism. In this study, chromosome painting using a W-specific probe and comparative chromosome mapping of repetitive sequences, including ribosomal clusters and 4 microsatellite motifs - (CA)(15), (GA)(15), (CG)(15), and (TTA)(10) -, were performed in 6 Characidium species, 5 of which possessed a heteromorphic ZW sex chromosome system. The W-specific probe showed hybridization signals on the W chromosome of all analyzed species, indicating homology among the W chromosomes. Remarkably, a single major rDNA-bearing chromosome pair was found in all species. The 18S rDNA localized to the sex chromosomes in C. lanei, C. timbuiense and C. pterostictum, while the major rDNA localized to one autosome pair in C. vidali and C. gomesi. In contrast, the number of 5S rDNA-bearing chromosomes varied. Notably, minor ribosomal clusters were identified in the W chromosome of C. vidali. Microsatellites were widely distributed across almost all chromosomes of the karyotypes, with a greater accumulation in the subtelomeric regions. However, clear differences in the abundance of each motif were detected in each species. In addition, the Z and W chromosomes showed the differential accumulation of distinct motifs. Our results revealed variability in the distribution of repetitive DNA sequences and their possible association with sex chromosome diversification in Characidium species. (C) 2015 S. Karger AG, BaselFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Dept Morfol, BR-18618970 Botucatu, SP, BrazilUniv Estadual Ponta Grossa, Dept Biol Estrutural Mol & Genet, Ponta Grossa, BrazilUniv Estadual Paulista, Dept Morfol, BR-18618970 Botucatu, SP, BrazilKargerUniversidade Estadual Paulista (Unesp)Universidade Estadual de Ponta Grossa (UEPG)Scacchetti, Priscilla C. [UNESP]Utsunomia, Ricardo [UNESP]Pansonato-Alves, Jose C. [UNESP]Vicari, Marcelo R.Artoni, Roberto F.Oliveira, Claudio [UNESP]Foresti, Fausto [UNESP]2018-11-26T16:17:03Z2018-11-26T16:17:03Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article136-143application/pdfhttp://dx.doi.org/10.1159/000437165Cytogenetic And Genome Research. Basel: Karger, v. 146, n. 2, p. 136-143, 2015.1424-8581http://hdl.handle.net/11449/16086510.1159/000437165WOS:000361848600007WOS000361848600007.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCytogenetic And Genome Research0,748info:eu-repo/semantics/openAccess2024-01-03T06:19:48Zoai:repositorio.unesp.br:11449/160865Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:59:08.306115Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chromosomal Mapping of Repetitive DNAs in Characidium (Teleostei, Characiformes): Genomic Organization and Diversification of ZW Sex Chromosomes |
title |
Chromosomal Mapping of Repetitive DNAs in Characidium (Teleostei, Characiformes): Genomic Organization and Diversification of ZW Sex Chromosomes |
spellingShingle |
Chromosomal Mapping of Repetitive DNAs in Characidium (Teleostei, Characiformes): Genomic Organization and Diversification of ZW Sex Chromosomes Scacchetti, Priscilla C. [UNESP] Chromosome painting Crenuchidae Fish cytogenetics Karyotype diversification |
title_short |
Chromosomal Mapping of Repetitive DNAs in Characidium (Teleostei, Characiformes): Genomic Organization and Diversification of ZW Sex Chromosomes |
title_full |
Chromosomal Mapping of Repetitive DNAs in Characidium (Teleostei, Characiformes): Genomic Organization and Diversification of ZW Sex Chromosomes |
title_fullStr |
Chromosomal Mapping of Repetitive DNAs in Characidium (Teleostei, Characiformes): Genomic Organization and Diversification of ZW Sex Chromosomes |
title_full_unstemmed |
Chromosomal Mapping of Repetitive DNAs in Characidium (Teleostei, Characiformes): Genomic Organization and Diversification of ZW Sex Chromosomes |
title_sort |
Chromosomal Mapping of Repetitive DNAs in Characidium (Teleostei, Characiformes): Genomic Organization and Diversification of ZW Sex Chromosomes |
author |
Scacchetti, Priscilla C. [UNESP] |
author_facet |
Scacchetti, Priscilla C. [UNESP] Utsunomia, Ricardo [UNESP] Pansonato-Alves, Jose C. [UNESP] Vicari, Marcelo R. Artoni, Roberto F. Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
author_role |
author |
author2 |
Utsunomia, Ricardo [UNESP] Pansonato-Alves, Jose C. [UNESP] Vicari, Marcelo R. Artoni, Roberto F. Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Ponta Grossa (UEPG) |
dc.contributor.author.fl_str_mv |
Scacchetti, Priscilla C. [UNESP] Utsunomia, Ricardo [UNESP] Pansonato-Alves, Jose C. [UNESP] Vicari, Marcelo R. Artoni, Roberto F. Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
dc.subject.por.fl_str_mv |
Chromosome painting Crenuchidae Fish cytogenetics Karyotype diversification |
topic |
Chromosome painting Crenuchidae Fish cytogenetics Karyotype diversification |
description |
The speciose neotropical genus Characidium has proven to be a good model for cytogenetic exploration. Representatives of this genus often have a conserved diploid chromosome number; some species exhibit a highly differentiated ZZ/ZW sex chromosome system, while others do not show any sex-related chromosome heteromorphism. In this study, chromosome painting using a W-specific probe and comparative chromosome mapping of repetitive sequences, including ribosomal clusters and 4 microsatellite motifs - (CA)(15), (GA)(15), (CG)(15), and (TTA)(10) -, were performed in 6 Characidium species, 5 of which possessed a heteromorphic ZW sex chromosome system. The W-specific probe showed hybridization signals on the W chromosome of all analyzed species, indicating homology among the W chromosomes. Remarkably, a single major rDNA-bearing chromosome pair was found in all species. The 18S rDNA localized to the sex chromosomes in C. lanei, C. timbuiense and C. pterostictum, while the major rDNA localized to one autosome pair in C. vidali and C. gomesi. In contrast, the number of 5S rDNA-bearing chromosomes varied. Notably, minor ribosomal clusters were identified in the W chromosome of C. vidali. Microsatellites were widely distributed across almost all chromosomes of the karyotypes, with a greater accumulation in the subtelomeric regions. However, clear differences in the abundance of each motif were detected in each species. In addition, the Z and W chromosomes showed the differential accumulation of distinct motifs. Our results revealed variability in the distribution of repetitive DNA sequences and their possible association with sex chromosome diversification in Characidium species. (C) 2015 S. Karger AG, Basel |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2018-11-26T16:17:03Z 2018-11-26T16:17:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1159/000437165 Cytogenetic And Genome Research. Basel: Karger, v. 146, n. 2, p. 136-143, 2015. 1424-8581 http://hdl.handle.net/11449/160865 10.1159/000437165 WOS:000361848600007 WOS000361848600007.pdf |
url |
http://dx.doi.org/10.1159/000437165 http://hdl.handle.net/11449/160865 |
identifier_str_mv |
Cytogenetic And Genome Research. Basel: Karger, v. 146, n. 2, p. 136-143, 2015. 1424-8581 10.1159/000437165 WOS:000361848600007 WOS000361848600007.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cytogenetic And Genome Research 0,748 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
136-143 application/pdf |
dc.publisher.none.fl_str_mv |
Karger |
publisher.none.fl_str_mv |
Karger |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129380982980608 |