Photodynamic therapy for American cutaneous leishmaniasis: The efficacy of methylene blue in hamsters experimentally infected with Leishmania (Leishmania) amazonensis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.exppara.2011.04.009 http://hdl.handle.net/11449/6537 |
Resumo: | The aim of this study was to investigate the effectiveness of Photodynamic Therapy (PDT) using Methylene Blue (MB) as the photosensitizing compound and a Light-Emitting Diode (LED) in American cutaneous leishmaniasis (ACL). Hamsters were experimentally infected with Leishmania (Leishmania) amazonensis. After the development of the lesions in the footpad, the animals were treated with MB three times a week for 3 months. Ten minutes after each application of MB, the lesions were irradiated with LED for 1 h. The lesions were evaluated weekly by the measurement of the hamster footpad thickness. At the end of the treatment the parasitic load was quantified in the regional lymph node of the hamsters. The treatment promoted a decrease in the thickness of infected footpad (P = 0.0001) and reduction in the parasitic load in the regional lymph node (P = 0.0007) of the animals from group treated with MB + LED. PDT using MB + LED in ACL caused by L. amazonensis shows a strong photodynamic effect. This therapy is very promising, once it is an inexpensive system and the own patient can apply it in their wound and in their house without the need of technical assistance. (C) 2011 Elsevier B.V. All rights reserved. |
id |
UNSP_305ea21dd69d22524482b2c7a30a3259 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/6537 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Photodynamic therapy for American cutaneous leishmaniasis: The efficacy of methylene blue in hamsters experimentally infected with Leishmania (Leishmania) amazonensisCutaneous leishmaniasisPhotodynamic TherapyLeishmania amazonensisMethylene BlueLight-Emitting-DiodeThe aim of this study was to investigate the effectiveness of Photodynamic Therapy (PDT) using Methylene Blue (MB) as the photosensitizing compound and a Light-Emitting Diode (LED) in American cutaneous leishmaniasis (ACL). Hamsters were experimentally infected with Leishmania (Leishmania) amazonensis. After the development of the lesions in the footpad, the animals were treated with MB three times a week for 3 months. Ten minutes after each application of MB, the lesions were irradiated with LED for 1 h. The lesions were evaluated weekly by the measurement of the hamster footpad thickness. At the end of the treatment the parasitic load was quantified in the regional lymph node of the hamsters. The treatment promoted a decrease in the thickness of infected footpad (P = 0.0001) and reduction in the parasitic load in the regional lymph node (P = 0.0007) of the animals from group treated with MB + LED. PDT using MB + LED in ACL caused by L. amazonensis shows a strong photodynamic effect. This therapy is very promising, once it is an inexpensive system and the own patient can apply it in their wound and in their house without the need of technical assistance. (C) 2011 Elsevier B.V. All rights reserved.Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Paraná (FAADCT/PR)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual de Maringá (UEM), Dept Anal Clin & Biomed, BR-87020900 Maringa, Parana, BrazilUniversidade Estadual de Maringá (UEM), Dept Quim, BR-87020900 Maringa, Parana, BrazilUniversidade Estadual de Maringá (UEM), Dept Farm & Farmacol, BR-87020900 Maringa, Parana, BrazilUniv São Paulo, Inst Quim, São Paulo, BrazilUniv Estadual Paulista, Dept Ciencias Biol, São Paulo, BrazilUniv Estadual Paulista, Dept Ciencias Biol, São Paulo, BrazilCNPq: 400618/2004-4 Rede BiomatsAcademic Press Inc. Elsevier B.V.Universidade Estadual de Maringá (UEM)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Peloi, Lilian SomenciGuerino Biondo, Carlos EduardoKimura, ElzaPoliti, Mario JoseCampana Lonardoni, Maria ValdrinezAlessi Aristides, Sandra MaraCunha Dorea, Regina Coeli [UNESP]Hioka, NoboruVerzignassi Silveira, Thais Gomes2014-05-20T13:22:20Z2014-05-20T13:22:20Z2011-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article353-356application/pdfhttp://dx.doi.org/10.1016/j.exppara.2011.04.009Experimental Parasitology. San Diego: Academic Press Inc. Elsevier B.V., v. 128, n. 4, p. 353-356, 2011.0014-4894http://hdl.handle.net/11449/653710.1016/j.exppara.2011.04.009WOS:000293670400009WOS000293670400009.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengExperimental Parasitology1.8210,635info:eu-repo/semantics/openAccess2023-10-06T06:01:14Zoai:repositorio.unesp.br:11449/6537Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:07:43.739854Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Photodynamic therapy for American cutaneous leishmaniasis: The efficacy of methylene blue in hamsters experimentally infected with Leishmania (Leishmania) amazonensis |
title |
Photodynamic therapy for American cutaneous leishmaniasis: The efficacy of methylene blue in hamsters experimentally infected with Leishmania (Leishmania) amazonensis |
spellingShingle |
Photodynamic therapy for American cutaneous leishmaniasis: The efficacy of methylene blue in hamsters experimentally infected with Leishmania (Leishmania) amazonensis Peloi, Lilian Somenci Cutaneous leishmaniasis Photodynamic Therapy Leishmania amazonensis Methylene Blue Light-Emitting-Diode |
title_short |
Photodynamic therapy for American cutaneous leishmaniasis: The efficacy of methylene blue in hamsters experimentally infected with Leishmania (Leishmania) amazonensis |
title_full |
Photodynamic therapy for American cutaneous leishmaniasis: The efficacy of methylene blue in hamsters experimentally infected with Leishmania (Leishmania) amazonensis |
title_fullStr |
Photodynamic therapy for American cutaneous leishmaniasis: The efficacy of methylene blue in hamsters experimentally infected with Leishmania (Leishmania) amazonensis |
title_full_unstemmed |
Photodynamic therapy for American cutaneous leishmaniasis: The efficacy of methylene blue in hamsters experimentally infected with Leishmania (Leishmania) amazonensis |
title_sort |
Photodynamic therapy for American cutaneous leishmaniasis: The efficacy of methylene blue in hamsters experimentally infected with Leishmania (Leishmania) amazonensis |
author |
Peloi, Lilian Somenci |
author_facet |
Peloi, Lilian Somenci Guerino Biondo, Carlos Eduardo Kimura, Elza Politi, Mario Jose Campana Lonardoni, Maria Valdrinez Alessi Aristides, Sandra Mara Cunha Dorea, Regina Coeli [UNESP] Hioka, Noboru Verzignassi Silveira, Thais Gomes |
author_role |
author |
author2 |
Guerino Biondo, Carlos Eduardo Kimura, Elza Politi, Mario Jose Campana Lonardoni, Maria Valdrinez Alessi Aristides, Sandra Mara Cunha Dorea, Regina Coeli [UNESP] Hioka, Noboru Verzignassi Silveira, Thais Gomes |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Maringá (UEM) Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Peloi, Lilian Somenci Guerino Biondo, Carlos Eduardo Kimura, Elza Politi, Mario Jose Campana Lonardoni, Maria Valdrinez Alessi Aristides, Sandra Mara Cunha Dorea, Regina Coeli [UNESP] Hioka, Noboru Verzignassi Silveira, Thais Gomes |
dc.subject.por.fl_str_mv |
Cutaneous leishmaniasis Photodynamic Therapy Leishmania amazonensis Methylene Blue Light-Emitting-Diode |
topic |
Cutaneous leishmaniasis Photodynamic Therapy Leishmania amazonensis Methylene Blue Light-Emitting-Diode |
description |
The aim of this study was to investigate the effectiveness of Photodynamic Therapy (PDT) using Methylene Blue (MB) as the photosensitizing compound and a Light-Emitting Diode (LED) in American cutaneous leishmaniasis (ACL). Hamsters were experimentally infected with Leishmania (Leishmania) amazonensis. After the development of the lesions in the footpad, the animals were treated with MB three times a week for 3 months. Ten minutes after each application of MB, the lesions were irradiated with LED for 1 h. The lesions were evaluated weekly by the measurement of the hamster footpad thickness. At the end of the treatment the parasitic load was quantified in the regional lymph node of the hamsters. The treatment promoted a decrease in the thickness of infected footpad (P = 0.0001) and reduction in the parasitic load in the regional lymph node (P = 0.0007) of the animals from group treated with MB + LED. PDT using MB + LED in ACL caused by L. amazonensis shows a strong photodynamic effect. This therapy is very promising, once it is an inexpensive system and the own patient can apply it in their wound and in their house without the need of technical assistance. (C) 2011 Elsevier B.V. All rights reserved. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-01 2014-05-20T13:22:20Z 2014-05-20T13:22:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.exppara.2011.04.009 Experimental Parasitology. San Diego: Academic Press Inc. Elsevier B.V., v. 128, n. 4, p. 353-356, 2011. 0014-4894 http://hdl.handle.net/11449/6537 10.1016/j.exppara.2011.04.009 WOS:000293670400009 WOS000293670400009.pdf |
url |
http://dx.doi.org/10.1016/j.exppara.2011.04.009 http://hdl.handle.net/11449/6537 |
identifier_str_mv |
Experimental Parasitology. San Diego: Academic Press Inc. Elsevier B.V., v. 128, n. 4, p. 353-356, 2011. 0014-4894 10.1016/j.exppara.2011.04.009 WOS:000293670400009 WOS000293670400009.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Experimental Parasitology 1.821 0,635 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
353-356 application/pdf |
dc.publisher.none.fl_str_mv |
Academic Press Inc. Elsevier B.V. |
publisher.none.fl_str_mv |
Academic Press Inc. Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128319159271424 |