Differential Responses of Tobacco to the Citrus Variegated Chlorosis and Coffee Stem Atrophy Strains of Xylella fastidiosa

Detalhes bibliográficos
Autor(a) principal: Lopes, Silvio A. [UNESP]
Data de Publicação: 2020
Outros Autores: Raiol-Junion, Laudecir L. [UNESP], Torres, Simone C. Z., Martins, Elaine C., Prado, Simone S., Beriam, Luis O. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1094/PHYTO-10-19-0374-R
http://hdl.handle.net/11449/195223
Resumo: Xylella fastidiosa comprises a diverse group of xylem-limited, insect-transmitted bacterial pathogens. In Brazil, the citrus variegated chlorosis (CVC) and coffee stem atrophy (CSA) diseases are caused by X. fastidiosa subspecies pauca transmitted by common insect vectors. No simple protocol allowing strain discrimination exists, making epidemiological studies, which are important for devising control measures, difficult to undertake. Here, we show that both strains can easily be distinguished based on the pattern of leaf symptoms that they induce on pin prick-inoculated tobacco seedlings, namely small orange lesions and large necrotic lesions induced by the CVC and CSA strains, respectively. These differential responses allowed us to investigate whether mixed strain infections would occur in citrus or coffee trees in the field. Seedlings were individually inoculated with X. fastidiosa colonies recovered from citrus or coffee plants from various locations at three different times. No mixed infections were detected. In two experiments, the citrus and coffee strains infected only their original hosts as well as tobacco. The usefulness of this tobacco bioassay as a tool to study X. fastidiosa spread was demonstrated. It provided evidence that, over the years, the CVC and CSA pathogens have remained limited to their original hosts, despite crop proximity and the presence of sharpshooter vectors that favor transmission of the bacteria to and between both host species.
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spelling Differential Responses of Tobacco to the Citrus Variegated Chlorosis and Coffee Stem Atrophy Strains of Xylella fastidiosabacteriologydisease managementepidemiologyetiologyinoculum sourcespopulation biologyXylella fastidiosa comprises a diverse group of xylem-limited, insect-transmitted bacterial pathogens. In Brazil, the citrus variegated chlorosis (CVC) and coffee stem atrophy (CSA) diseases are caused by X. fastidiosa subspecies pauca transmitted by common insect vectors. No simple protocol allowing strain discrimination exists, making epidemiological studies, which are important for devising control measures, difficult to undertake. Here, we show that both strains can easily be distinguished based on the pattern of leaf symptoms that they induce on pin prick-inoculated tobacco seedlings, namely small orange lesions and large necrotic lesions induced by the CVC and CSA strains, respectively. These differential responses allowed us to investigate whether mixed strain infections would occur in citrus or coffee trees in the field. Seedlings were individually inoculated with X. fastidiosa colonies recovered from citrus or coffee plants from various locations at three different times. No mixed infections were detected. In two experiments, the citrus and coffee strains infected only their original hosts as well as tobacco. The usefulness of this tobacco bioassay as a tool to study X. fastidiosa spread was demonstrated. It provided evidence that, over the years, the CVC and CSA pathogens have remained limited to their original hosts, despite crop proximity and the presence of sharpshooter vectors that favor transmission of the bacteria to and between both host species.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Jaboticabal, SP, BrazilFundo Def Citricultura, Araraquara, SP, BrazilUniv Ribeirao Preto, Ribeirao Preto, SP, BrazilEmpresa Brasileira Pesquisa Agr, Jaguariuna, SP, BrazilInst Biol, Campinas, SP, BrazilUniv Estadual Paulista, Jaboticabal, SP, BrazilFAPESP: 1999/04343-0FAPESP: 1999/04345-3Amer Phytopathological SocUniversidade Estadual Paulista (Unesp)Fundo Def CitriculturaUniv Ribeirao PretoEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade de São Paulo (USP)Lopes, Silvio A. [UNESP]Raiol-Junion, Laudecir L. [UNESP]Torres, Simone C. Z.Martins, Elaine C.Prado, Simone S.Beriam, Luis O. S.2020-12-10T17:27:29Z2020-12-10T17:27:29Z2020-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article567-573http://dx.doi.org/10.1094/PHYTO-10-19-0374-RPhytopathology. St Paul: Amer Phytopathological Soc, v. 110, n. 3, p. 567-573, 2020.0031-949Xhttp://hdl.handle.net/11449/19522310.1094/PHYTO-10-19-0374-RWOS:000516771400005Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhytopathologyinfo:eu-repo/semantics/openAccess2021-10-23T06:52:13Zoai:repositorio.unesp.br:11449/195223Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:28:19.351002Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Differential Responses of Tobacco to the Citrus Variegated Chlorosis and Coffee Stem Atrophy Strains of Xylella fastidiosa
title Differential Responses of Tobacco to the Citrus Variegated Chlorosis and Coffee Stem Atrophy Strains of Xylella fastidiosa
spellingShingle Differential Responses of Tobacco to the Citrus Variegated Chlorosis and Coffee Stem Atrophy Strains of Xylella fastidiosa
Lopes, Silvio A. [UNESP]
bacteriology
disease management
epidemiology
etiology
inoculum sources
population biology
title_short Differential Responses of Tobacco to the Citrus Variegated Chlorosis and Coffee Stem Atrophy Strains of Xylella fastidiosa
title_full Differential Responses of Tobacco to the Citrus Variegated Chlorosis and Coffee Stem Atrophy Strains of Xylella fastidiosa
title_fullStr Differential Responses of Tobacco to the Citrus Variegated Chlorosis and Coffee Stem Atrophy Strains of Xylella fastidiosa
title_full_unstemmed Differential Responses of Tobacco to the Citrus Variegated Chlorosis and Coffee Stem Atrophy Strains of Xylella fastidiosa
title_sort Differential Responses of Tobacco to the Citrus Variegated Chlorosis and Coffee Stem Atrophy Strains of Xylella fastidiosa
author Lopes, Silvio A. [UNESP]
author_facet Lopes, Silvio A. [UNESP]
Raiol-Junion, Laudecir L. [UNESP]
Torres, Simone C. Z.
Martins, Elaine C.
Prado, Simone S.
Beriam, Luis O. S.
author_role author
author2 Raiol-Junion, Laudecir L. [UNESP]
Torres, Simone C. Z.
Martins, Elaine C.
Prado, Simone S.
Beriam, Luis O. S.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Fundo Def Citricultura
Univ Ribeirao Preto
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Lopes, Silvio A. [UNESP]
Raiol-Junion, Laudecir L. [UNESP]
Torres, Simone C. Z.
Martins, Elaine C.
Prado, Simone S.
Beriam, Luis O. S.
dc.subject.por.fl_str_mv bacteriology
disease management
epidemiology
etiology
inoculum sources
population biology
topic bacteriology
disease management
epidemiology
etiology
inoculum sources
population biology
description Xylella fastidiosa comprises a diverse group of xylem-limited, insect-transmitted bacterial pathogens. In Brazil, the citrus variegated chlorosis (CVC) and coffee stem atrophy (CSA) diseases are caused by X. fastidiosa subspecies pauca transmitted by common insect vectors. No simple protocol allowing strain discrimination exists, making epidemiological studies, which are important for devising control measures, difficult to undertake. Here, we show that both strains can easily be distinguished based on the pattern of leaf symptoms that they induce on pin prick-inoculated tobacco seedlings, namely small orange lesions and large necrotic lesions induced by the CVC and CSA strains, respectively. These differential responses allowed us to investigate whether mixed strain infections would occur in citrus or coffee trees in the field. Seedlings were individually inoculated with X. fastidiosa colonies recovered from citrus or coffee plants from various locations at three different times. No mixed infections were detected. In two experiments, the citrus and coffee strains infected only their original hosts as well as tobacco. The usefulness of this tobacco bioassay as a tool to study X. fastidiosa spread was demonstrated. It provided evidence that, over the years, the CVC and CSA pathogens have remained limited to their original hosts, despite crop proximity and the presence of sharpshooter vectors that favor transmission of the bacteria to and between both host species.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-10T17:27:29Z
2020-12-10T17:27:29Z
2020-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1094/PHYTO-10-19-0374-R
Phytopathology. St Paul: Amer Phytopathological Soc, v. 110, n. 3, p. 567-573, 2020.
0031-949X
http://hdl.handle.net/11449/195223
10.1094/PHYTO-10-19-0374-R
WOS:000516771400005
url http://dx.doi.org/10.1094/PHYTO-10-19-0374-R
http://hdl.handle.net/11449/195223
identifier_str_mv Phytopathology. St Paul: Amer Phytopathological Soc, v. 110, n. 3, p. 567-573, 2020.
0031-949X
10.1094/PHYTO-10-19-0374-R
WOS:000516771400005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Phytopathology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 567-573
dc.publisher.none.fl_str_mv Amer Phytopathological Soc
publisher.none.fl_str_mv Amer Phytopathological Soc
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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