Effect of sodium hexametaphosphate and quercetin, associated or not with fluoride, on dentin erosion in vitro
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2022.105541 http://hdl.handle.net/11449/247620 |
Resumo: | Objective: to investigate the ability of solutions containing sodium hexametaphosphate, fluoride and quercetin, alone or in association, to prevent dentin erosion and to inhibit matrix metalloproteinases -2 and -9 activity using in vitro protocols. Design: Root dentin blocks (n = 96) were prepared and divided into 8 experimental groups (n = 12/group), according to the solutions to be tested: Placebo; 0.24% sodium fluoride (F); 1.0% sodium hexametaphosphate (HMP); 0.03% quercetin (QC); F+HMP; F+QC; HMP+QC; and F+HMP+QC. Erosive challenges were performed 4×/day for 5 days. Specimens were treated with the respective solutions for one minute, twice a day. Next, dentin loss (profilometry) and integrated hardness area in depth (KHN × µm) were determined. The antiproteolytic potential was assessed by gelatin zymography. Dentin erosion results (log10-transformed) were submitted to one-way ANOVA, followed by Tukey's test. Integrated hardness area in depth data (raw) were submitted to two-way, repeated-measures ANOVA, followed by Holm-Sidak's test (p<0.05). Results: Dentin erosion was significantly lower for F+HMP+QC than for all other treatments. At the shallowest depths (5–30 µm), blocks treated with F+HMP+QC had the highest integrated hardness area in depth values. All treatments completely inhibited matrix metalloproteinases-2 activity, except for the group QC (77% inhibition). For matrix metalloproteinases-9, all HMP-containing solutions or F+QC promoted total antiproteolytic activity. Conclusion: The association of fluoride, sodium hexametaphosphate, and quercetin must be considered a valuable strategy for novel product formulation for home and professional use, considering its superior protective effects against dentin erosion and its antiproteolytic potential. |
id |
UNSP_3369d963d22ab5be5b93ffd45e2a66ab |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/247620 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Effect of sodium hexametaphosphate and quercetin, associated or not with fluoride, on dentin erosion in vitroDentinMatrix metalloproteinase inhibitorsPolyphosphatesQuercetinTooth erosionObjective: to investigate the ability of solutions containing sodium hexametaphosphate, fluoride and quercetin, alone or in association, to prevent dentin erosion and to inhibit matrix metalloproteinases -2 and -9 activity using in vitro protocols. Design: Root dentin blocks (n = 96) were prepared and divided into 8 experimental groups (n = 12/group), according to the solutions to be tested: Placebo; 0.24% sodium fluoride (F); 1.0% sodium hexametaphosphate (HMP); 0.03% quercetin (QC); F+HMP; F+QC; HMP+QC; and F+HMP+QC. Erosive challenges were performed 4×/day for 5 days. Specimens were treated with the respective solutions for one minute, twice a day. Next, dentin loss (profilometry) and integrated hardness area in depth (KHN × µm) were determined. The antiproteolytic potential was assessed by gelatin zymography. Dentin erosion results (log10-transformed) were submitted to one-way ANOVA, followed by Tukey's test. Integrated hardness area in depth data (raw) were submitted to two-way, repeated-measures ANOVA, followed by Holm-Sidak's test (p<0.05). Results: Dentin erosion was significantly lower for F+HMP+QC than for all other treatments. At the shallowest depths (5–30 µm), blocks treated with F+HMP+QC had the highest integrated hardness area in depth values. All treatments completely inhibited matrix metalloproteinases-2 activity, except for the group QC (77% inhibition). For matrix metalloproteinases-9, all HMP-containing solutions or F+QC promoted total antiproteolytic activity. Conclusion: The association of fluoride, sodium hexametaphosphate, and quercetin must be considered a valuable strategy for novel product formulation for home and professional use, considering its superior protective effects against dentin erosion and its antiproteolytic potential.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP), SPDepartment of Cariology Restorative Sciences and Endodontics University of Michigan, MIDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo, SPDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP), SPFAPESP: #2019/02354-0CAPES: 001Universidade Estadual Paulista (UNESP)University of MichiganUniversidade de São Paulo (USP)Capalbo, Letícia Cabrera [UNESP]Delbem, Alberto Carlos Botazzo [UNESP]Dal-Fabbro, RenanInácio, Kelly Karinade Oliveira, Rodrigo CardosoPessan, Juliano Pelim [UNESP]2023-07-29T13:21:01Z2023-07-29T13:21:01Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.archoralbio.2022.105541Archives of Oral Biology, v. 143.1879-15060003-9969http://hdl.handle.net/11449/24762010.1016/j.archoralbio.2022.1055412-s2.0-85138031246Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biologyinfo:eu-repo/semantics/openAccess2023-07-29T13:21:01Zoai:repositorio.unesp.br:11449/247620Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:09:51.089241Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of sodium hexametaphosphate and quercetin, associated or not with fluoride, on dentin erosion in vitro |
title |
Effect of sodium hexametaphosphate and quercetin, associated or not with fluoride, on dentin erosion in vitro |
spellingShingle |
Effect of sodium hexametaphosphate and quercetin, associated or not with fluoride, on dentin erosion in vitro Capalbo, Letícia Cabrera [UNESP] Dentin Matrix metalloproteinase inhibitors Polyphosphates Quercetin Tooth erosion |
title_short |
Effect of sodium hexametaphosphate and quercetin, associated or not with fluoride, on dentin erosion in vitro |
title_full |
Effect of sodium hexametaphosphate and quercetin, associated or not with fluoride, on dentin erosion in vitro |
title_fullStr |
Effect of sodium hexametaphosphate and quercetin, associated or not with fluoride, on dentin erosion in vitro |
title_full_unstemmed |
Effect of sodium hexametaphosphate and quercetin, associated or not with fluoride, on dentin erosion in vitro |
title_sort |
Effect of sodium hexametaphosphate and quercetin, associated or not with fluoride, on dentin erosion in vitro |
author |
Capalbo, Letícia Cabrera [UNESP] |
author_facet |
Capalbo, Letícia Cabrera [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Dal-Fabbro, Renan Inácio, Kelly Karina de Oliveira, Rodrigo Cardoso Pessan, Juliano Pelim [UNESP] |
author_role |
author |
author2 |
Delbem, Alberto Carlos Botazzo [UNESP] Dal-Fabbro, Renan Inácio, Kelly Karina de Oliveira, Rodrigo Cardoso Pessan, Juliano Pelim [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Michigan Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Capalbo, Letícia Cabrera [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Dal-Fabbro, Renan Inácio, Kelly Karina de Oliveira, Rodrigo Cardoso Pessan, Juliano Pelim [UNESP] |
dc.subject.por.fl_str_mv |
Dentin Matrix metalloproteinase inhibitors Polyphosphates Quercetin Tooth erosion |
topic |
Dentin Matrix metalloproteinase inhibitors Polyphosphates Quercetin Tooth erosion |
description |
Objective: to investigate the ability of solutions containing sodium hexametaphosphate, fluoride and quercetin, alone or in association, to prevent dentin erosion and to inhibit matrix metalloproteinases -2 and -9 activity using in vitro protocols. Design: Root dentin blocks (n = 96) were prepared and divided into 8 experimental groups (n = 12/group), according to the solutions to be tested: Placebo; 0.24% sodium fluoride (F); 1.0% sodium hexametaphosphate (HMP); 0.03% quercetin (QC); F+HMP; F+QC; HMP+QC; and F+HMP+QC. Erosive challenges were performed 4×/day for 5 days. Specimens were treated with the respective solutions for one minute, twice a day. Next, dentin loss (profilometry) and integrated hardness area in depth (KHN × µm) were determined. The antiproteolytic potential was assessed by gelatin zymography. Dentin erosion results (log10-transformed) were submitted to one-way ANOVA, followed by Tukey's test. Integrated hardness area in depth data (raw) were submitted to two-way, repeated-measures ANOVA, followed by Holm-Sidak's test (p<0.05). Results: Dentin erosion was significantly lower for F+HMP+QC than for all other treatments. At the shallowest depths (5–30 µm), blocks treated with F+HMP+QC had the highest integrated hardness area in depth values. All treatments completely inhibited matrix metalloproteinases-2 activity, except for the group QC (77% inhibition). For matrix metalloproteinases-9, all HMP-containing solutions or F+QC promoted total antiproteolytic activity. Conclusion: The association of fluoride, sodium hexametaphosphate, and quercetin must be considered a valuable strategy for novel product formulation for home and professional use, considering its superior protective effects against dentin erosion and its antiproteolytic potential. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-01 2023-07-29T13:21:01Z 2023-07-29T13:21:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2022.105541 Archives of Oral Biology, v. 143. 1879-1506 0003-9969 http://hdl.handle.net/11449/247620 10.1016/j.archoralbio.2022.105541 2-s2.0-85138031246 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2022.105541 http://hdl.handle.net/11449/247620 |
identifier_str_mv |
Archives of Oral Biology, v. 143. 1879-1506 0003-9969 10.1016/j.archoralbio.2022.105541 2-s2.0-85138031246 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128470541139968 |