Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jobm.202200439 http://hdl.handle.net/11449/246256 |
Resumo: | To find effective silver nanoparticles (AgNPs) for control of phytopathogens, in this study, two strains of actinomycetes isolated from the soil of the Brazilian biome Caatinga (Caat5–35) and from mangrove sediment (Canv1–58) were utilized. The strains were identified by using the 16S rRNA gene sequencing as Streptomyces sp., related to Streptomyces mimosus species. The obtained AgNPs were coded as AgNPs 35 and AgNPs58 and characterized by size and morphology using dynamic light scattering, zeta potential, transmission electron microscopy, and Fourier transformed infrared (FTIR). The antifungal activity of the AgNPs35 and AgNPs58 was evaluated in vitro by the minimal inhibitory concentration (MIC) assay on the phytopathogens, Alternaria solani, Alternaria alternata, and Colletotrichum gloeosporioides. The phytotoxic effect was evaluated by the germination rate and seedling growth of rice (Oryza sativa). AgNPs35 and AgNPs58 showed surface plasmon resonance and average sizes of 30 and 60 nm, respectively. Both AgNPs presented spherical shape and the FTIR analysis confirmed the presence of functional groups such as free amines and hydroxyls of biomolecules bounded to the external layer of the nanoparticles. Both AgNPs inhibited the growth of the three phytopathogens tested, and A. alternate was the most sensible (MIC ≤ 4 µM). Moreover, the AgNPs35 and AgNPs58 did not induce phytotoxic effects on the germination and development of rice seedlings. In conclusion, these AgNPs are promising candidates to biocontrol of these phytopathogens without endangering rice plants. |
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Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogensactinomycetesbiocontrolbiogenic silver nanoparticlesphytotoxicityrice (Oryza sativa)To find effective silver nanoparticles (AgNPs) for control of phytopathogens, in this study, two strains of actinomycetes isolated from the soil of the Brazilian biome Caatinga (Caat5–35) and from mangrove sediment (Canv1–58) were utilized. The strains were identified by using the 16S rRNA gene sequencing as Streptomyces sp., related to Streptomyces mimosus species. The obtained AgNPs were coded as AgNPs 35 and AgNPs58 and characterized by size and morphology using dynamic light scattering, zeta potential, transmission electron microscopy, and Fourier transformed infrared (FTIR). The antifungal activity of the AgNPs35 and AgNPs58 was evaluated in vitro by the minimal inhibitory concentration (MIC) assay on the phytopathogens, Alternaria solani, Alternaria alternata, and Colletotrichum gloeosporioides. The phytotoxic effect was evaluated by the germination rate and seedling growth of rice (Oryza sativa). AgNPs35 and AgNPs58 showed surface plasmon resonance and average sizes of 30 and 60 nm, respectively. Both AgNPs presented spherical shape and the FTIR analysis confirmed the presence of functional groups such as free amines and hydroxyls of biomolecules bounded to the external layer of the nanoparticles. Both AgNPs inhibited the growth of the three phytopathogens tested, and A. alternate was the most sensible (MIC ≤ 4 µM). Moreover, the AgNPs35 and AgNPs58 did not induce phytotoxic effects on the germination and development of rice seedlings. In conclusion, these AgNPs are promising candidates to biocontrol of these phytopathogens without endangering rice plants.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Development and Innovation Laboratory Instituto ButantanInstitute of Biosciences São Paulo State University (UNESP)Institute of Advanced Sea Studies (IEAMAR) São Paulo State University (UNESP)LABMEM Microbiology Department ICB II Universidade de São Paulo (USP)Environmental Microbiology Laboratory EMBRAPA Meio AmbienteLinking Landscape Environment Agriculture and Food (LEAF) Instituto Superior de Agronomia Universidade de LisboaInstitute of Biosciences São Paulo State University (UNESP)Institute of Advanced Sea Studies (IEAMAR) São Paulo State University (UNESP)FAPESP: 2010/50186-5FAPESP: 2018/04258-6FAPESP: 2020/12867-2Instituto ButantanUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade de LisboaZwar, Ingrid P. [UNESP]Trotta, Caterina do Valle [UNESP]Ziotti, Ana B. S. [UNESP]Lima Neto, Milton [UNESP]Araújo, Welington L.de Melo, Itamar S.Ottoni, Cristiane A. [UNESP]de Souza, Ana O.2023-07-29T12:35:55Z2023-07-29T12:35:55Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article64-74http://dx.doi.org/10.1002/jobm.202200439Journal of Basic Microbiology, v. 63, n. 1, p. 64-74, 2023.1521-40280233-111Xhttp://hdl.handle.net/11449/24625610.1002/jobm.2022004392-s2.0-85141516936Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Basic Microbiologyinfo:eu-repo/semantics/openAccess2023-07-29T12:35:55Zoai:repositorio.unesp.br:11449/246256Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:08:33.111171Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens |
title |
Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens |
spellingShingle |
Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens Zwar, Ingrid P. [UNESP] actinomycetes biocontrol biogenic silver nanoparticles phytotoxicity rice (Oryza sativa) |
title_short |
Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens |
title_full |
Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens |
title_fullStr |
Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens |
title_full_unstemmed |
Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens |
title_sort |
Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens |
author |
Zwar, Ingrid P. [UNESP] |
author_facet |
Zwar, Ingrid P. [UNESP] Trotta, Caterina do Valle [UNESP] Ziotti, Ana B. S. [UNESP] Lima Neto, Milton [UNESP] Araújo, Welington L. de Melo, Itamar S. Ottoni, Cristiane A. [UNESP] de Souza, Ana O. |
author_role |
author |
author2 |
Trotta, Caterina do Valle [UNESP] Ziotti, Ana B. S. [UNESP] Lima Neto, Milton [UNESP] Araújo, Welington L. de Melo, Itamar S. Ottoni, Cristiane A. [UNESP] de Souza, Ana O. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Instituto Butantan Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Zwar, Ingrid P. [UNESP] Trotta, Caterina do Valle [UNESP] Ziotti, Ana B. S. [UNESP] Lima Neto, Milton [UNESP] Araújo, Welington L. de Melo, Itamar S. Ottoni, Cristiane A. [UNESP] de Souza, Ana O. |
dc.subject.por.fl_str_mv |
actinomycetes biocontrol biogenic silver nanoparticles phytotoxicity rice (Oryza sativa) |
topic |
actinomycetes biocontrol biogenic silver nanoparticles phytotoxicity rice (Oryza sativa) |
description |
To find effective silver nanoparticles (AgNPs) for control of phytopathogens, in this study, two strains of actinomycetes isolated from the soil of the Brazilian biome Caatinga (Caat5–35) and from mangrove sediment (Canv1–58) were utilized. The strains were identified by using the 16S rRNA gene sequencing as Streptomyces sp., related to Streptomyces mimosus species. The obtained AgNPs were coded as AgNPs 35 and AgNPs58 and characterized by size and morphology using dynamic light scattering, zeta potential, transmission electron microscopy, and Fourier transformed infrared (FTIR). The antifungal activity of the AgNPs35 and AgNPs58 was evaluated in vitro by the minimal inhibitory concentration (MIC) assay on the phytopathogens, Alternaria solani, Alternaria alternata, and Colletotrichum gloeosporioides. The phytotoxic effect was evaluated by the germination rate and seedling growth of rice (Oryza sativa). AgNPs35 and AgNPs58 showed surface plasmon resonance and average sizes of 30 and 60 nm, respectively. Both AgNPs presented spherical shape and the FTIR analysis confirmed the presence of functional groups such as free amines and hydroxyls of biomolecules bounded to the external layer of the nanoparticles. Both AgNPs inhibited the growth of the three phytopathogens tested, and A. alternate was the most sensible (MIC ≤ 4 µM). Moreover, the AgNPs35 and AgNPs58 did not induce phytotoxic effects on the germination and development of rice seedlings. In conclusion, these AgNPs are promising candidates to biocontrol of these phytopathogens without endangering rice plants. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:35:55Z 2023-07-29T12:35:55Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jobm.202200439 Journal of Basic Microbiology, v. 63, n. 1, p. 64-74, 2023. 1521-4028 0233-111X http://hdl.handle.net/11449/246256 10.1002/jobm.202200439 2-s2.0-85141516936 |
url |
http://dx.doi.org/10.1002/jobm.202200439 http://hdl.handle.net/11449/246256 |
identifier_str_mv |
Journal of Basic Microbiology, v. 63, n. 1, p. 64-74, 2023. 1521-4028 0233-111X 10.1002/jobm.202200439 2-s2.0-85141516936 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Basic Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
64-74 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129396684357632 |