Efeito da mitomicina c no epitélio ciliar estudo experimental em coelhos com microscopia eletrônica de varredura e de transmissão
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.sboportal.org.br/rbo/2006/rbo_jan_fev_2006.pdf http://hdl.handle.net/11449/69391 |
Resumo: | Purpose: To evaluate and correlate in the rabbit the possible changes caused by mitomycin C under the scleral flap in the ciliary epithelium with transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Methods: The eyes of 32 albino rabbits were studied and divided in 4 experimental groups. The right eye (RE) was intended for the experimental groups and the left eye (LE) for the controls. Group I (G1) was formed by 8 eyes that received 0,5 mg/ml of mitomycin C under the scleral flap and were examined after 15 days. Group II (G2) differed from G1 only in the time of the exam, after 30 days. Group III (G3) was formed by 8 eyes that received 0,2 mg/ml of mitomycin C under the scleral flap and were examined after 15 days. Group IV (G4) differed from group 3 just in the time of the exam, after 30 days. In each eye the internal ciliary epithelium were examined with TEM. Results: The following changes in the internal ciliary epithelium were observed in groups G2, G3, and G4 with TEM: discontinuous and irregular basement membrane, more irregular and electron-dense nucleous, enlargement among interdigitation, edematous mitochondria and myelin figures. These alterations were not found in all the animals of the groups. Group G 1 did not present alterations. Roughness in groups G 1, G2, G3 and G4 were observed with SEM. In groups G 1 and G2 continuity solutions were also observed. Conclusion: Mitomycin C in 0,2 mg/ml and 0,5 mg/ml concentrations caused changes in the internal ciliary epithelium 15 and 30 days after, with TEM and SEM. There was no correlation between dosage, time and with TEM and SEM. |
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Efeito da mitomicina c no epitélio ciliar estudo experimental em coelhos com microscopia eletrônica de varredura e de transmissãoEffect of mitomycin c on the ciliary epithelium experimental study in rabbits with transmission and scanning electron microscopyCiliary body (ciliary epithelium)/drug effectsMicroscopy, electronMicroscopy, electron, scanningMitomycinRabbitsPurpose: To evaluate and correlate in the rabbit the possible changes caused by mitomycin C under the scleral flap in the ciliary epithelium with transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Methods: The eyes of 32 albino rabbits were studied and divided in 4 experimental groups. The right eye (RE) was intended for the experimental groups and the left eye (LE) for the controls. Group I (G1) was formed by 8 eyes that received 0,5 mg/ml of mitomycin C under the scleral flap and were examined after 15 days. Group II (G2) differed from G1 only in the time of the exam, after 30 days. Group III (G3) was formed by 8 eyes that received 0,2 mg/ml of mitomycin C under the scleral flap and were examined after 15 days. Group IV (G4) differed from group 3 just in the time of the exam, after 30 days. In each eye the internal ciliary epithelium were examined with TEM. Results: The following changes in the internal ciliary epithelium were observed in groups G2, G3, and G4 with TEM: discontinuous and irregular basement membrane, more irregular and electron-dense nucleous, enlargement among interdigitation, edematous mitochondria and myelin figures. These alterations were not found in all the animals of the groups. Group G 1 did not present alterations. Roughness in groups G 1, G2, G3 and G4 were observed with SEM. In groups G 1 and G2 continuity solutions were also observed. Conclusion: Mitomycin C in 0,2 mg/ml and 0,5 mg/ml concentrations caused changes in the internal ciliary epithelium 15 and 30 days after, with TEM and SEM. There was no correlation between dosage, time and with TEM and SEM.Departamento de Oftalmologia, Otorrinolaringologia Cirurgia de Cabeça e PescoçoFaculdade de Medicina Universidade Estadual de Sao Paulo Julio Mesquita Filho - UNESP, Botucatu, SPDepartamento de Morfologia Instituto de Biociências Universidade Estadual de São Paulo Julio Mesquita Filho - UNESP, Botucatu, SPFaculdade de Medicina Universidade Estadual de Sao Paulo Julio Mesquita Filho - UNESP, Botucatu, SPDepartamento de Morfologia Instituto de Biociências Universidade Estadual de São Paulo Julio Mesquita Filho - UNESP, Botucatu, SPUniversidade Estadual Paulista (Unesp)Silva, Maria Rosa Bet de Moraes [UNESP]Gregório, Elisa Aparecida [UNESP]2014-05-27T11:22:20Z2014-05-27T11:22:20Z2006-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article18-27application/pdfhttp://www.sboportal.org.br/rbo/2006/rbo_jan_fev_2006.pdfRevista Brasileira de Oftalmologia, v. 65, n. 1, p. 18-27, 2006.0034-72801982-8551http://hdl.handle.net/11449/693912-s2.0-676491583052-s2.0-67649158305.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Oftalmologia0,147info:eu-repo/semantics/openAccess2023-10-28T06:11:47Zoai:repositorio.unesp.br:11449/69391Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:18:11.148335Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Efeito da mitomicina c no epitélio ciliar estudo experimental em coelhos com microscopia eletrônica de varredura e de transmissão Effect of mitomycin c on the ciliary epithelium experimental study in rabbits with transmission and scanning electron microscopy |
title |
Efeito da mitomicina c no epitélio ciliar estudo experimental em coelhos com microscopia eletrônica de varredura e de transmissão |
spellingShingle |
Efeito da mitomicina c no epitélio ciliar estudo experimental em coelhos com microscopia eletrônica de varredura e de transmissão Silva, Maria Rosa Bet de Moraes [UNESP] Ciliary body (ciliary epithelium)/drug effects Microscopy, electron Microscopy, electron, scanning Mitomycin Rabbits |
title_short |
Efeito da mitomicina c no epitélio ciliar estudo experimental em coelhos com microscopia eletrônica de varredura e de transmissão |
title_full |
Efeito da mitomicina c no epitélio ciliar estudo experimental em coelhos com microscopia eletrônica de varredura e de transmissão |
title_fullStr |
Efeito da mitomicina c no epitélio ciliar estudo experimental em coelhos com microscopia eletrônica de varredura e de transmissão |
title_full_unstemmed |
Efeito da mitomicina c no epitélio ciliar estudo experimental em coelhos com microscopia eletrônica de varredura e de transmissão |
title_sort |
Efeito da mitomicina c no epitélio ciliar estudo experimental em coelhos com microscopia eletrônica de varredura e de transmissão |
author |
Silva, Maria Rosa Bet de Moraes [UNESP] |
author_facet |
Silva, Maria Rosa Bet de Moraes [UNESP] Gregório, Elisa Aparecida [UNESP] |
author_role |
author |
author2 |
Gregório, Elisa Aparecida [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, Maria Rosa Bet de Moraes [UNESP] Gregório, Elisa Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Ciliary body (ciliary epithelium)/drug effects Microscopy, electron Microscopy, electron, scanning Mitomycin Rabbits |
topic |
Ciliary body (ciliary epithelium)/drug effects Microscopy, electron Microscopy, electron, scanning Mitomycin Rabbits |
description |
Purpose: To evaluate and correlate in the rabbit the possible changes caused by mitomycin C under the scleral flap in the ciliary epithelium with transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Methods: The eyes of 32 albino rabbits were studied and divided in 4 experimental groups. The right eye (RE) was intended for the experimental groups and the left eye (LE) for the controls. Group I (G1) was formed by 8 eyes that received 0,5 mg/ml of mitomycin C under the scleral flap and were examined after 15 days. Group II (G2) differed from G1 only in the time of the exam, after 30 days. Group III (G3) was formed by 8 eyes that received 0,2 mg/ml of mitomycin C under the scleral flap and were examined after 15 days. Group IV (G4) differed from group 3 just in the time of the exam, after 30 days. In each eye the internal ciliary epithelium were examined with TEM. Results: The following changes in the internal ciliary epithelium were observed in groups G2, G3, and G4 with TEM: discontinuous and irregular basement membrane, more irregular and electron-dense nucleous, enlargement among interdigitation, edematous mitochondria and myelin figures. These alterations were not found in all the animals of the groups. Group G 1 did not present alterations. Roughness in groups G 1, G2, G3 and G4 were observed with SEM. In groups G 1 and G2 continuity solutions were also observed. Conclusion: Mitomycin C in 0,2 mg/ml and 0,5 mg/ml concentrations caused changes in the internal ciliary epithelium 15 and 30 days after, with TEM and SEM. There was no correlation between dosage, time and with TEM and SEM. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-12-01 2014-05-27T11:22:20Z 2014-05-27T11:22:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.sboportal.org.br/rbo/2006/rbo_jan_fev_2006.pdf Revista Brasileira de Oftalmologia, v. 65, n. 1, p. 18-27, 2006. 0034-7280 1982-8551 http://hdl.handle.net/11449/69391 2-s2.0-67649158305 2-s2.0-67649158305.pdf |
url |
http://www.sboportal.org.br/rbo/2006/rbo_jan_fev_2006.pdf http://hdl.handle.net/11449/69391 |
identifier_str_mv |
Revista Brasileira de Oftalmologia, v. 65, n. 1, p. 18-27, 2006. 0034-7280 1982-8551 2-s2.0-67649158305 2-s2.0-67649158305.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista Brasileira de Oftalmologia 0,147 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
18-27 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128223424282624 |