Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1071/RD15067 http://hdl.handle.net/11449/173483 |
Resumo: | Proper oocyte maturation is crucial for subsequent embryo development; however, oocyte mitochondrial and lipid-droplet behaviour are still poorly understood. Although excessive lipid accumulation during in vitro production (IVP) of bovine embryos has been linked with impaired cryotolerance, lipid oxidation is essential for adequate energy supply. Fetal bovine serum (FBS) and bovine serum albumin (BSA) are supplements used during IVP, containing high and low lipid content, respectively. This study aimed to understand how these supplements influence oocyte mitochondrial and lipid behaviour during in vitro maturation (IVM) in comparison to in vivo maturation, as well as their influence on development rates and embryo lipid accumulation during IVP. We demonstrate that only in vivo-matured oocytes maintained correlation between lipid content and active mitochondria. IVM media containing FBS increased total lipid content 18-fold and resulted in higher lipid accumulation in oocytes when compared with media with BSA. IVM using a lower FBS concentration combined with BSA resulted in satisfactory maturation and embryo development and also reduced lipid accumulation in blastocysts. In conclusion, IVM causes changes in mitochondrial and lipid dynamics, which may have negative effects on oocyte development rates and embryo lipid accumulation. Moreover, decreasing FBS concentrations during IVM may reduce embryo lipid accumulation without affecting production rates. |
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Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryosin vitro production (IVP)lipid dropletmitochondriareproductionProper oocyte maturation is crucial for subsequent embryo development; however, oocyte mitochondrial and lipid-droplet behaviour are still poorly understood. Although excessive lipid accumulation during in vitro production (IVP) of bovine embryos has been linked with impaired cryotolerance, lipid oxidation is essential for adequate energy supply. Fetal bovine serum (FBS) and bovine serum albumin (BSA) are supplements used during IVP, containing high and low lipid content, respectively. This study aimed to understand how these supplements influence oocyte mitochondrial and lipid behaviour during in vitro maturation (IVM) in comparison to in vivo maturation, as well as their influence on development rates and embryo lipid accumulation during IVP. We demonstrate that only in vivo-matured oocytes maintained correlation between lipid content and active mitochondria. IVM media containing FBS increased total lipid content 18-fold and resulted in higher lipid accumulation in oocytes when compared with media with BSA. IVM using a lower FBS concentration combined with BSA resulted in satisfactory maturation and embryo development and also reduced lipid accumulation in blastocysts. In conclusion, IVM causes changes in mitochondrial and lipid dynamics, which may have negative effects on oocyte development rates and embryo lipid accumulation. Moreover, decreasing FBS concentrations during IVM may reduce embryo lipid accumulation without affecting production rates.Departamento de Medicina Veterinária Faculdade de Zootecnia e Engenharia de Alimentos USP, Avenida Duque de Caxias Norte, 225Departamento de Medicina Veterinária Preventiva e Reproduçao Animal Faculdade de Ciencias Agrárias e Veterinárias UNESP, Via de Acesso Prof. Paulo D. Castellane SNEmpresa Brasileira de Pesquisa Agropecuária Embrapa Amazônia Oriental, Travessa Dr. Enéas Pinheiro s/nDepartamento de Apoio Produção e Saúde Animal Faculdade de Medicina Veterinária de Araçatuba UNESP, Rua Clóvis Pestana, 793Departamento de Biologia Celular e Molecular e de Bioagentes Patogênicos Faculdade de Medicina de Ribeirão Preto USP, Avenida Bandeirantes, 3900Departamento de Medicina Veterinária Preventiva e Reproduçao Animal Faculdade de Ciencias Agrárias e Veterinárias UNESP, Via de Acesso Prof. Paulo D. Castellane SNDepartamento de Apoio Produção e Saúde Animal Faculdade de Medicina Veterinária de Araçatuba UNESP, Rua Clóvis Pestana, 793Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Del Collado, Maite [UNESP]Saraiva, Naiara Z.Lopes, Flavia L. [UNESP]Gaspar, Roberta C. [UNESP]Padilha, Luciana C. [UNESP]Costa, Roberta R.Rossi, Guilherme F. [UNESP]Vantini, Roberta [UNESP]Garcia, Joaquim M. [UNESP]2018-12-11T17:05:50Z2018-12-11T17:05:50Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1721-1732application/pdfhttp://dx.doi.org/10.1071/RD15067Reproduction, Fertility and Development, v. 28, n. 11, p. 1721-1732, 2016.1448-59901031-3613http://hdl.handle.net/11449/17348310.1071/RD150672-s2.0-849879232612-s2.0-84987923261.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproduction, Fertility and Development0,681info:eu-repo/semantics/openAccess2024-09-04T19:15:38Zoai:repositorio.unesp.br:11449/173483Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos |
title |
Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos |
spellingShingle |
Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos Del Collado, Maite [UNESP] in vitro production (IVP) lipid droplet mitochondria reproduction |
title_short |
Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos |
title_full |
Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos |
title_fullStr |
Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos |
title_full_unstemmed |
Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos |
title_sort |
Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos |
author |
Del Collado, Maite [UNESP] |
author_facet |
Del Collado, Maite [UNESP] Saraiva, Naiara Z. Lopes, Flavia L. [UNESP] Gaspar, Roberta C. [UNESP] Padilha, Luciana C. [UNESP] Costa, Roberta R. Rossi, Guilherme F. [UNESP] Vantini, Roberta [UNESP] Garcia, Joaquim M. [UNESP] |
author_role |
author |
author2 |
Saraiva, Naiara Z. Lopes, Flavia L. [UNESP] Gaspar, Roberta C. [UNESP] Padilha, Luciana C. [UNESP] Costa, Roberta R. Rossi, Guilherme F. [UNESP] Vantini, Roberta [UNESP] Garcia, Joaquim M. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Del Collado, Maite [UNESP] Saraiva, Naiara Z. Lopes, Flavia L. [UNESP] Gaspar, Roberta C. [UNESP] Padilha, Luciana C. [UNESP] Costa, Roberta R. Rossi, Guilherme F. [UNESP] Vantini, Roberta [UNESP] Garcia, Joaquim M. [UNESP] |
dc.subject.por.fl_str_mv |
in vitro production (IVP) lipid droplet mitochondria reproduction |
topic |
in vitro production (IVP) lipid droplet mitochondria reproduction |
description |
Proper oocyte maturation is crucial for subsequent embryo development; however, oocyte mitochondrial and lipid-droplet behaviour are still poorly understood. Although excessive lipid accumulation during in vitro production (IVP) of bovine embryos has been linked with impaired cryotolerance, lipid oxidation is essential for adequate energy supply. Fetal bovine serum (FBS) and bovine serum albumin (BSA) are supplements used during IVP, containing high and low lipid content, respectively. This study aimed to understand how these supplements influence oocyte mitochondrial and lipid behaviour during in vitro maturation (IVM) in comparison to in vivo maturation, as well as their influence on development rates and embryo lipid accumulation during IVP. We demonstrate that only in vivo-matured oocytes maintained correlation between lipid content and active mitochondria. IVM media containing FBS increased total lipid content 18-fold and resulted in higher lipid accumulation in oocytes when compared with media with BSA. IVM using a lower FBS concentration combined with BSA resulted in satisfactory maturation and embryo development and also reduced lipid accumulation in blastocysts. In conclusion, IVM causes changes in mitochondrial and lipid dynamics, which may have negative effects on oocyte development rates and embryo lipid accumulation. Moreover, decreasing FBS concentrations during IVM may reduce embryo lipid accumulation without affecting production rates. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T17:05:50Z 2018-12-11T17:05:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1071/RD15067 Reproduction, Fertility and Development, v. 28, n. 11, p. 1721-1732, 2016. 1448-5990 1031-3613 http://hdl.handle.net/11449/173483 10.1071/RD15067 2-s2.0-84987923261 2-s2.0-84987923261.pdf |
url |
http://dx.doi.org/10.1071/RD15067 http://hdl.handle.net/11449/173483 |
identifier_str_mv |
Reproduction, Fertility and Development, v. 28, n. 11, p. 1721-1732, 2016. 1448-5990 1031-3613 10.1071/RD15067 2-s2.0-84987923261 2-s2.0-84987923261.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproduction, Fertility and Development 0,681 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1721-1732 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021389101432832 |