Highly absorptive dressing composed of natural latex loaded with alginate for exudate control and healing of diabetic wounds

Detalhes bibliográficos
Autor(a) principal: Barros, Natan Roberto [UNESP]
Data de Publicação: 2021
Outros Autores: Ahadian, Samad, Tebon, Peyton, Rudge, Marilza Vieira Cunha [UNESP], Barbosa, Angélica Mércia Pascon [UNESP], Herculano, Rondinelli Donizetti [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.msec.2020.111589
http://hdl.handle.net/11449/206703
Resumo: Wounds can take longer to heal in diabetic patients, increasing the risk of infections and other complications. The most common wounds in diabetic patients are diabetic foot ulcers, a severe complication associated with diabetes mellitus. The United States alone spends $18.7 billion annually on care for these wounds including pain and infection management. If improperly managed, infected lesions may require amputation. The enormous cost associated with wound care and the dire consequences if not cared for properly, emphasize the need to develop strategies to accelerate the healing of diabetic foot wounds. Natural rubber latex (NRL), extracted from Hevea brasiliensis (the rubber tree), has been widely applied as a carrier system for several pharmacologically active compounds. Furthermore, it has been shown to encourage angiogenesis, facilitate cell adhesion, and accelerate wound healing. When NRL dressings are applied to wounds of diabetic patients, exudate release is upregulated. The production of exudate is essential to wound healing as it provides the nutrients, proteins, cells, and environment required for regeneration. Despite its benefits, it is necessary to control excess exudate to avoid prolonged healing resulting from dermatitis, maceration of the wound edges, and lesion growth. In order to solve the problem of excessive exudate release induced by NRL membrane application, we aimed to regulate humidity by absorbing excess exudate and increase water vapor transmission. We developed a highly absorptive, permeable, alginate loaded NRL dressing. Adding alginate to NRL membranes, swelling was increased up to 80-fold, absorbing 4.80 g of water per gram of dry membrane. Moreover, water vapor transmission was improved drastically as the material transmitted 480% more water vapor than pure NRL membranes. Furthermore, in vitro tests demonstrated not only that the membranes are biocompatible, but that they also enhance cell proliferation. Through a cell proliferation assay, we observed that fibroblast proliferation was improved by the presence of NRL while the keratinocytes benefit from the presence of alginate. The NRL-alginate dressings have great potential to improve diabetic wound care by accelerating the healing process.
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spelling Highly absorptive dressing composed of natural latex loaded with alginate for exudate control and healing of diabetic woundsAlginateBiomaterialCell proliferationExudateNatural rubber latexWound healingWounds can take longer to heal in diabetic patients, increasing the risk of infections and other complications. The most common wounds in diabetic patients are diabetic foot ulcers, a severe complication associated with diabetes mellitus. The United States alone spends $18.7 billion annually on care for these wounds including pain and infection management. If improperly managed, infected lesions may require amputation. The enormous cost associated with wound care and the dire consequences if not cared for properly, emphasize the need to develop strategies to accelerate the healing of diabetic foot wounds. Natural rubber latex (NRL), extracted from Hevea brasiliensis (the rubber tree), has been widely applied as a carrier system for several pharmacologically active compounds. Furthermore, it has been shown to encourage angiogenesis, facilitate cell adhesion, and accelerate wound healing. When NRL dressings are applied to wounds of diabetic patients, exudate release is upregulated. The production of exudate is essential to wound healing as it provides the nutrients, proteins, cells, and environment required for regeneration. Despite its benefits, it is necessary to control excess exudate to avoid prolonged healing resulting from dermatitis, maceration of the wound edges, and lesion growth. In order to solve the problem of excessive exudate release induced by NRL membrane application, we aimed to regulate humidity by absorbing excess exudate and increase water vapor transmission. We developed a highly absorptive, permeable, alginate loaded NRL dressing. Adding alginate to NRL membranes, swelling was increased up to 80-fold, absorbing 4.80 g of water per gram of dry membrane. Moreover, water vapor transmission was improved drastically as the material transmitted 480% more water vapor than pure NRL membranes. Furthermore, in vitro tests demonstrated not only that the membranes are biocompatible, but that they also enhance cell proliferation. Through a cell proliferation assay, we observed that fibroblast proliferation was improved by the presence of NRL while the keratinocytes benefit from the presence of alginate. The NRL-alginate dressings have great potential to improve diabetic wound care by accelerating the healing process.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Bioprocess and Biotechnology Engineering São Paulo State University (Unesp) School of Pharmaceutical SciencesDepartment of Biochemistry and Organic Chemistry São Paulo State University (Unesp) Institute of ChemistryTerasaki Institute for Biomedical InnovationCenter for Minimally Invasive Therapeutics (C-MIT) University of California-Los AngelesDepartment of Bioengineering Henry Samueli School of Engineering and Applied Sciences University of California-Los AngelesDepartment of Gynecology and Obstetrics São Paulo State University (Unesp) School of MedicineDepartment of Physiotherapy and Occupational Therapy São Paulo State University (Unesp) School of Philosophy and ScienceDepartment of Bioprocess and Biotechnology Engineering São Paulo State University (Unesp) School of Pharmaceutical SciencesDepartment of Biochemistry and Organic Chemistry São Paulo State University (Unesp) Institute of ChemistryDepartment of Gynecology and Obstetrics São Paulo State University (Unesp) School of MedicineDepartment of Physiotherapy and Occupational Therapy São Paulo State University (Unesp) School of Philosophy and ScienceFAPESP: 2016/01743-5FAPESP: 2017/02518-8FAPESP: 2017/19603-8Universidade Estadual Paulista (Unesp)Terasaki Institute for Biomedical InnovationUniversity of California-Los AngelesBarros, Natan Roberto [UNESP]Ahadian, SamadTebon, PeytonRudge, Marilza Vieira Cunha [UNESP]Barbosa, Angélica Mércia Pascon [UNESP]Herculano, Rondinelli Donizetti [UNESP]2021-06-25T10:36:45Z2021-06-25T10:36:45Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.msec.2020.111589Materials Science and Engineering C, v. 119.1873-01910928-4931http://hdl.handle.net/11449/20670310.1016/j.msec.2020.1115892-s2.0-85092899485Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Science and Engineering Cinfo:eu-repo/semantics/openAccess2024-08-16T14:06:42Zoai:repositorio.unesp.br:11449/206703Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:06:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Highly absorptive dressing composed of natural latex loaded with alginate for exudate control and healing of diabetic wounds
title Highly absorptive dressing composed of natural latex loaded with alginate for exudate control and healing of diabetic wounds
spellingShingle Highly absorptive dressing composed of natural latex loaded with alginate for exudate control and healing of diabetic wounds
Barros, Natan Roberto [UNESP]
Alginate
Biomaterial
Cell proliferation
Exudate
Natural rubber latex
Wound healing
title_short Highly absorptive dressing composed of natural latex loaded with alginate for exudate control and healing of diabetic wounds
title_full Highly absorptive dressing composed of natural latex loaded with alginate for exudate control and healing of diabetic wounds
title_fullStr Highly absorptive dressing composed of natural latex loaded with alginate for exudate control and healing of diabetic wounds
title_full_unstemmed Highly absorptive dressing composed of natural latex loaded with alginate for exudate control and healing of diabetic wounds
title_sort Highly absorptive dressing composed of natural latex loaded with alginate for exudate control and healing of diabetic wounds
author Barros, Natan Roberto [UNESP]
author_facet Barros, Natan Roberto [UNESP]
Ahadian, Samad
Tebon, Peyton
Rudge, Marilza Vieira Cunha [UNESP]
Barbosa, Angélica Mércia Pascon [UNESP]
Herculano, Rondinelli Donizetti [UNESP]
author_role author
author2 Ahadian, Samad
Tebon, Peyton
Rudge, Marilza Vieira Cunha [UNESP]
Barbosa, Angélica Mércia Pascon [UNESP]
Herculano, Rondinelli Donizetti [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Terasaki Institute for Biomedical Innovation
University of California-Los Angeles
dc.contributor.author.fl_str_mv Barros, Natan Roberto [UNESP]
Ahadian, Samad
Tebon, Peyton
Rudge, Marilza Vieira Cunha [UNESP]
Barbosa, Angélica Mércia Pascon [UNESP]
Herculano, Rondinelli Donizetti [UNESP]
dc.subject.por.fl_str_mv Alginate
Biomaterial
Cell proliferation
Exudate
Natural rubber latex
Wound healing
topic Alginate
Biomaterial
Cell proliferation
Exudate
Natural rubber latex
Wound healing
description Wounds can take longer to heal in diabetic patients, increasing the risk of infections and other complications. The most common wounds in diabetic patients are diabetic foot ulcers, a severe complication associated with diabetes mellitus. The United States alone spends $18.7 billion annually on care for these wounds including pain and infection management. If improperly managed, infected lesions may require amputation. The enormous cost associated with wound care and the dire consequences if not cared for properly, emphasize the need to develop strategies to accelerate the healing of diabetic foot wounds. Natural rubber latex (NRL), extracted from Hevea brasiliensis (the rubber tree), has been widely applied as a carrier system for several pharmacologically active compounds. Furthermore, it has been shown to encourage angiogenesis, facilitate cell adhesion, and accelerate wound healing. When NRL dressings are applied to wounds of diabetic patients, exudate release is upregulated. The production of exudate is essential to wound healing as it provides the nutrients, proteins, cells, and environment required for regeneration. Despite its benefits, it is necessary to control excess exudate to avoid prolonged healing resulting from dermatitis, maceration of the wound edges, and lesion growth. In order to solve the problem of excessive exudate release induced by NRL membrane application, we aimed to regulate humidity by absorbing excess exudate and increase water vapor transmission. We developed a highly absorptive, permeable, alginate loaded NRL dressing. Adding alginate to NRL membranes, swelling was increased up to 80-fold, absorbing 4.80 g of water per gram of dry membrane. Moreover, water vapor transmission was improved drastically as the material transmitted 480% more water vapor than pure NRL membranes. Furthermore, in vitro tests demonstrated not only that the membranes are biocompatible, but that they also enhance cell proliferation. Through a cell proliferation assay, we observed that fibroblast proliferation was improved by the presence of NRL while the keratinocytes benefit from the presence of alginate. The NRL-alginate dressings have great potential to improve diabetic wound care by accelerating the healing process.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:36:45Z
2021-06-25T10:36:45Z
2021-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.msec.2020.111589
Materials Science and Engineering C, v. 119.
1873-0191
0928-4931
http://hdl.handle.net/11449/206703
10.1016/j.msec.2020.111589
2-s2.0-85092899485
url http://dx.doi.org/10.1016/j.msec.2020.111589
http://hdl.handle.net/11449/206703
identifier_str_mv Materials Science and Engineering C, v. 119.
1873-0191
0928-4931
10.1016/j.msec.2020.111589
2-s2.0-85092899485
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Materials Science and Engineering C
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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