Topical sodium alendronate combined or not with photodynamic therapy as an adjunct to scaling and root planing: Histochemical and immunohistochemical study in rats
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/jre.12777 http://hdl.handle.net/11449/197052 |
Resumo: | Objective The purpose of this study was to evaluate influence of topical sodium alendronate (ALN), photodynamic therapy (aPDT), or a combination thereof as adjuvant to scaling and root planing (SRP) in the treatment of experimental periodontitis in rats. Background Therapeutic protocols to control periodontitis progression that aim to equalize bacterial action and load with tissue immune response are well addressed in current scientific research. Methods Experimental periodontitis was induced in 96 rats with a ligature around the mandibular left first molar. After 7 days, ligature was removed and animals were treated according to the following experimental groups (n = 8): control-SRP plus saline solution; ALN-SRP plus ALN; aPDT-SRP plus methylene blue irrigation, followed by low-level laser therapy (LLLT); and ALN/aPDT-SRP plus ALN and methylene blue irrigation followed by LLLT. The animals were euthanized at 7, 15, and 30 days after treatments. Collagen maturation (picrosirius red staining) and immunohistochemical analyses (TRAP, RANKL and osteoprotegerin [OPG]) were performed. Data were submitted to statistical analysis (P < .05). Results At 7 days, group ALN presented a significantly higher number of TRAP-positive cells and percentage of immature collagen fibers than group ALN/aPDT, while group ALN/aPDT presented a significantly higher percentage of mature collagen fibers than group ALN. At 30 days, group ALN presented significantly lower percentage of immature collagen fibers and higher percentage of mature collagen fibers than control. Conclusion It can be concluded that topical use of ALN coadjutant to SRP, alone or combined with aPDT, enhanced collagen maturation and reduced osteoclastogenesis during the healing of experimental periodontitis. |
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Repositório Institucional da UNESP |
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2946 |
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Topical sodium alendronate combined or not with photodynamic therapy as an adjunct to scaling and root planing: Histochemical and immunohistochemical study in ratscollagenosteoclastperiodontal therapyphotochemotherapyObjective The purpose of this study was to evaluate influence of topical sodium alendronate (ALN), photodynamic therapy (aPDT), or a combination thereof as adjuvant to scaling and root planing (SRP) in the treatment of experimental periodontitis in rats. Background Therapeutic protocols to control periodontitis progression that aim to equalize bacterial action and load with tissue immune response are well addressed in current scientific research. Methods Experimental periodontitis was induced in 96 rats with a ligature around the mandibular left first molar. After 7 days, ligature was removed and animals were treated according to the following experimental groups (n = 8): control-SRP plus saline solution; ALN-SRP plus ALN; aPDT-SRP plus methylene blue irrigation, followed by low-level laser therapy (LLLT); and ALN/aPDT-SRP plus ALN and methylene blue irrigation followed by LLLT. The animals were euthanized at 7, 15, and 30 days after treatments. Collagen maturation (picrosirius red staining) and immunohistochemical analyses (TRAP, RANKL and osteoprotegerin [OPG]) were performed. Data were submitted to statistical analysis (P < .05). Results At 7 days, group ALN presented a significantly higher number of TRAP-positive cells and percentage of immature collagen fibers than group ALN/aPDT, while group ALN/aPDT presented a significantly higher percentage of mature collagen fibers than group ALN. At 30 days, group ALN presented significantly lower percentage of immature collagen fibers and higher percentage of mature collagen fibers than control. Conclusion It can be concluded that topical use of ALN coadjutant to SRP, alone or combined with aPDT, enhanced collagen maturation and reduced osteoclastogenesis during the healing of experimental periodontitis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)University of Western Sao PauloCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Western Sao Paulo, Dent Sch Presidente Prudente, Grad Program Dent GPD Masters Degree, Presidente Prudente, BrazilUniv Fed Pelotas, Grad Program Dent, Pelotas, RS, BrazilUniv Estadual Paulista, Dent Sch Aracatuba, Dept Basic Sci, Aracatuba, BrazilUniv Estadual Paulista, Dent Sch Aracatuba, Dept Basic Sci, Aracatuba, BrazilFAPESP: 2016/04163-0University of Western Sao Paulo: 3080University of Western Sao Paulo: 3771Wiley-BlackwellUniv Western Sao PauloUniv Fed PelotasUniversidade Estadual Paulista (Unesp)Santinoni, Carolina S.Silveira, Felipe M.Caldeira, Marcela L.Genaro, VitorMartins, Thiago M.Amaral, Caril C. F. doMaia, Luciana P.Mori, Graziela G.Ervolino, Edilson [UNESP]Pola, Natalia M.2020-12-10T20:04:37Z2020-12-10T20:04:37Z2020-07-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9http://dx.doi.org/10.1111/jre.12777Journal Of Periodontal Research. Hoboken: Wiley, 9 p., 2020.0022-3484http://hdl.handle.net/11449/19705210.1111/jre.12777WOS:000546551400001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Periodontal Researchinfo:eu-repo/semantics/openAccess2021-10-23T10:37:06Zoai:repositorio.unesp.br:11449/197052Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:58:45.964691Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Topical sodium alendronate combined or not with photodynamic therapy as an adjunct to scaling and root planing: Histochemical and immunohistochemical study in rats |
title |
Topical sodium alendronate combined or not with photodynamic therapy as an adjunct to scaling and root planing: Histochemical and immunohistochemical study in rats |
spellingShingle |
Topical sodium alendronate combined or not with photodynamic therapy as an adjunct to scaling and root planing: Histochemical and immunohistochemical study in rats Santinoni, Carolina S. collagen osteoclast periodontal therapy photochemotherapy |
title_short |
Topical sodium alendronate combined or not with photodynamic therapy as an adjunct to scaling and root planing: Histochemical and immunohistochemical study in rats |
title_full |
Topical sodium alendronate combined or not with photodynamic therapy as an adjunct to scaling and root planing: Histochemical and immunohistochemical study in rats |
title_fullStr |
Topical sodium alendronate combined or not with photodynamic therapy as an adjunct to scaling and root planing: Histochemical and immunohistochemical study in rats |
title_full_unstemmed |
Topical sodium alendronate combined or not with photodynamic therapy as an adjunct to scaling and root planing: Histochemical and immunohistochemical study in rats |
title_sort |
Topical sodium alendronate combined or not with photodynamic therapy as an adjunct to scaling and root planing: Histochemical and immunohistochemical study in rats |
author |
Santinoni, Carolina S. |
author_facet |
Santinoni, Carolina S. Silveira, Felipe M. Caldeira, Marcela L. Genaro, Vitor Martins, Thiago M. Amaral, Caril C. F. do Maia, Luciana P. Mori, Graziela G. Ervolino, Edilson [UNESP] Pola, Natalia M. |
author_role |
author |
author2 |
Silveira, Felipe M. Caldeira, Marcela L. Genaro, Vitor Martins, Thiago M. Amaral, Caril C. F. do Maia, Luciana P. Mori, Graziela G. Ervolino, Edilson [UNESP] Pola, Natalia M. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Western Sao Paulo Univ Fed Pelotas Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Santinoni, Carolina S. Silveira, Felipe M. Caldeira, Marcela L. Genaro, Vitor Martins, Thiago M. Amaral, Caril C. F. do Maia, Luciana P. Mori, Graziela G. Ervolino, Edilson [UNESP] Pola, Natalia M. |
dc.subject.por.fl_str_mv |
collagen osteoclast periodontal therapy photochemotherapy |
topic |
collagen osteoclast periodontal therapy photochemotherapy |
description |
Objective The purpose of this study was to evaluate influence of topical sodium alendronate (ALN), photodynamic therapy (aPDT), or a combination thereof as adjuvant to scaling and root planing (SRP) in the treatment of experimental periodontitis in rats. Background Therapeutic protocols to control periodontitis progression that aim to equalize bacterial action and load with tissue immune response are well addressed in current scientific research. Methods Experimental periodontitis was induced in 96 rats with a ligature around the mandibular left first molar. After 7 days, ligature was removed and animals were treated according to the following experimental groups (n = 8): control-SRP plus saline solution; ALN-SRP plus ALN; aPDT-SRP plus methylene blue irrigation, followed by low-level laser therapy (LLLT); and ALN/aPDT-SRP plus ALN and methylene blue irrigation followed by LLLT. The animals were euthanized at 7, 15, and 30 days after treatments. Collagen maturation (picrosirius red staining) and immunohistochemical analyses (TRAP, RANKL and osteoprotegerin [OPG]) were performed. Data were submitted to statistical analysis (P < .05). Results At 7 days, group ALN presented a significantly higher number of TRAP-positive cells and percentage of immature collagen fibers than group ALN/aPDT, while group ALN/aPDT presented a significantly higher percentage of mature collagen fibers than group ALN. At 30 days, group ALN presented significantly lower percentage of immature collagen fibers and higher percentage of mature collagen fibers than control. Conclusion It can be concluded that topical use of ALN coadjutant to SRP, alone or combined with aPDT, enhanced collagen maturation and reduced osteoclastogenesis during the healing of experimental periodontitis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T20:04:37Z 2020-12-10T20:04:37Z 2020-07-09 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/jre.12777 Journal Of Periodontal Research. Hoboken: Wiley, 9 p., 2020. 0022-3484 http://hdl.handle.net/11449/197052 10.1111/jre.12777 WOS:000546551400001 |
url |
http://dx.doi.org/10.1111/jre.12777 http://hdl.handle.net/11449/197052 |
identifier_str_mv |
Journal Of Periodontal Research. Hoboken: Wiley, 9 p., 2020. 0022-3484 10.1111/jre.12777 WOS:000546551400001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Periodontal Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129380423041024 |