Alterations in prostate morphogenesis in male rat offspring after maternal exposure to Di-n-butyl-phthalate (DBP)

Detalhes bibliográficos
Autor(a) principal: de Mello Santos, Talita [UNESP]
Data de Publicação: 2017
Outros Autores: da Silveira, Lívia Teresa Ribeiro [UNESP], Rinaldi, Jaqueline Carvalho [UNESP], Scarano, Wellerson Rodrigo [UNESP], Domeniconi, Raquel Fantin [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.reprotox.2017.03.010
http://hdl.handle.net/11449/178747
Resumo: Prostate morphogenesis is regulated by androgens hormones and modulated by morphogenetic proteins such as Bone Morphogenetic Proteins (BMPs). This study aims to investigate the effects on prostate development in male offspring and differentiation after gestational and lactational maternal exposure to Di-n-butyl-phthalate (DBP), an important environmental contamination. Pregnant Wistar rats received 100 or 500 mg/kg of DBP (DBP100 and DBP500), by gavage, from gestation day 15 (GD15) until postnatal day 21 (PND21). The pups were euthanized on PND1 and PND21. Anogenital distance and testosterone levels decreased in animals from exposed mothers (DBP100 and 500) on PND1. A three-dimensional reconstruction model of the prostatic urethra showed reduction in the prostatic buds in the DBP500 group. AR expression and α-actin immunoreactivity decreased, and BMP-4 expression was lower on PND1 for DBP500. These results showed that DBP exposure, especially at a higher dose, delayed prostate morphogenesis by reducing the testosterone/AR axis and BMP-4 expression.
id UNSP_4226fa3244c1a75ebc8985aa8325cda4
oai_identifier_str oai:repositorio.unesp.br:11449/178747
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Alterations in prostate morphogenesis in male rat offspring after maternal exposure to Di-n-butyl-phthalate (DBP)Androgen receptorAntiandrogenicDi-n-butyl-phthalate (DBP) BMP-4Prostate developmentTestosteroneα-actinProstate morphogenesis is regulated by androgens hormones and modulated by morphogenetic proteins such as Bone Morphogenetic Proteins (BMPs). This study aims to investigate the effects on prostate development in male offspring and differentiation after gestational and lactational maternal exposure to Di-n-butyl-phthalate (DBP), an important environmental contamination. Pregnant Wistar rats received 100 or 500 mg/kg of DBP (DBP100 and DBP500), by gavage, from gestation day 15 (GD15) until postnatal day 21 (PND21). The pups were euthanized on PND1 and PND21. Anogenital distance and testosterone levels decreased in animals from exposed mothers (DBP100 and 500) on PND1. A three-dimensional reconstruction model of the prostatic urethra showed reduction in the prostatic buds in the DBP500 group. AR expression and α-actin immunoreactivity decreased, and BMP-4 expression was lower on PND1 for DBP500. These results showed that DBP exposure, especially at a higher dose, delayed prostate morphogenesis by reducing the testosterone/AR axis and BMP-4 expression.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Anatomy Institute of Biosciences Univi Estadual Paulista/UNESPDepartment of Morphology Institute of Biosciences Univi Estadual Paulista/UNESPDepartment of Anatomy Institute of Biosciences Univi Estadual Paulista/UNESPDepartment of Morphology Institute of Biosciences Univi Estadual Paulista/UNESPFAPESP: 2013/07203-4Universidade Estadual Paulista (Unesp)de Mello Santos, Talita [UNESP]da Silveira, Lívia Teresa Ribeiro [UNESP]Rinaldi, Jaqueline Carvalho [UNESP]Scarano, Wellerson Rodrigo [UNESP]Domeniconi, Raquel Fantin [UNESP]2018-12-11T17:31:55Z2018-12-11T17:31:55Z2017-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article254-264application/pdfhttp://dx.doi.org/10.1016/j.reprotox.2017.03.010Reproductive Toxicology, v. 69, p. 254-264.1873-17080890-6238http://hdl.handle.net/11449/17874710.1016/j.reprotox.2017.03.0102-s2.0-850163239082-s2.0-85016323908.pdf54817565282994690000-0003-2938-010XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproductive Toxicology0,846info:eu-repo/semantics/openAccess2024-01-13T06:30:21Zoai:repositorio.unesp.br:11449/178747Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:49:04.324663Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Alterations in prostate morphogenesis in male rat offspring after maternal exposure to Di-n-butyl-phthalate (DBP)
title Alterations in prostate morphogenesis in male rat offspring after maternal exposure to Di-n-butyl-phthalate (DBP)
spellingShingle Alterations in prostate morphogenesis in male rat offspring after maternal exposure to Di-n-butyl-phthalate (DBP)
de Mello Santos, Talita [UNESP]
Androgen receptor
Antiandrogenic
Di-n-butyl-phthalate (DBP) BMP-4
Prostate development
Testosterone
α-actin
title_short Alterations in prostate morphogenesis in male rat offspring after maternal exposure to Di-n-butyl-phthalate (DBP)
title_full Alterations in prostate morphogenesis in male rat offspring after maternal exposure to Di-n-butyl-phthalate (DBP)
title_fullStr Alterations in prostate morphogenesis in male rat offspring after maternal exposure to Di-n-butyl-phthalate (DBP)
title_full_unstemmed Alterations in prostate morphogenesis in male rat offspring after maternal exposure to Di-n-butyl-phthalate (DBP)
title_sort Alterations in prostate morphogenesis in male rat offspring after maternal exposure to Di-n-butyl-phthalate (DBP)
author de Mello Santos, Talita [UNESP]
author_facet de Mello Santos, Talita [UNESP]
da Silveira, Lívia Teresa Ribeiro [UNESP]
Rinaldi, Jaqueline Carvalho [UNESP]
Scarano, Wellerson Rodrigo [UNESP]
Domeniconi, Raquel Fantin [UNESP]
author_role author
author2 da Silveira, Lívia Teresa Ribeiro [UNESP]
Rinaldi, Jaqueline Carvalho [UNESP]
Scarano, Wellerson Rodrigo [UNESP]
Domeniconi, Raquel Fantin [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv de Mello Santos, Talita [UNESP]
da Silveira, Lívia Teresa Ribeiro [UNESP]
Rinaldi, Jaqueline Carvalho [UNESP]
Scarano, Wellerson Rodrigo [UNESP]
Domeniconi, Raquel Fantin [UNESP]
dc.subject.por.fl_str_mv Androgen receptor
Antiandrogenic
Di-n-butyl-phthalate (DBP) BMP-4
Prostate development
Testosterone
α-actin
topic Androgen receptor
Antiandrogenic
Di-n-butyl-phthalate (DBP) BMP-4
Prostate development
Testosterone
α-actin
description Prostate morphogenesis is regulated by androgens hormones and modulated by morphogenetic proteins such as Bone Morphogenetic Proteins (BMPs). This study aims to investigate the effects on prostate development in male offspring and differentiation after gestational and lactational maternal exposure to Di-n-butyl-phthalate (DBP), an important environmental contamination. Pregnant Wistar rats received 100 or 500 mg/kg of DBP (DBP100 and DBP500), by gavage, from gestation day 15 (GD15) until postnatal day 21 (PND21). The pups were euthanized on PND1 and PND21. Anogenital distance and testosterone levels decreased in animals from exposed mothers (DBP100 and 500) on PND1. A three-dimensional reconstruction model of the prostatic urethra showed reduction in the prostatic buds in the DBP500 group. AR expression and α-actin immunoreactivity decreased, and BMP-4 expression was lower on PND1 for DBP500. These results showed that DBP exposure, especially at a higher dose, delayed prostate morphogenesis by reducing the testosterone/AR axis and BMP-4 expression.
publishDate 2017
dc.date.none.fl_str_mv 2017-04-01
2018-12-11T17:31:55Z
2018-12-11T17:31:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.reprotox.2017.03.010
Reproductive Toxicology, v. 69, p. 254-264.
1873-1708
0890-6238
http://hdl.handle.net/11449/178747
10.1016/j.reprotox.2017.03.010
2-s2.0-85016323908
2-s2.0-85016323908.pdf
5481756528299469
0000-0003-2938-010X
url http://dx.doi.org/10.1016/j.reprotox.2017.03.010
http://hdl.handle.net/11449/178747
identifier_str_mv Reproductive Toxicology, v. 69, p. 254-264.
1873-1708
0890-6238
10.1016/j.reprotox.2017.03.010
2-s2.0-85016323908
2-s2.0-85016323908.pdf
5481756528299469
0000-0003-2938-010X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Reproductive Toxicology
0,846
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 254-264
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129465174196224

Registros relacionados