Evaluation of the posterior insular cortex involvement in anxiogenic response to emotional stress in male rats: Functional topography along the rostrocaudal axis

Detalhes bibliográficos
Autor(a) principal: Vitorio, Alex S. [UNESP]
Data de Publicação: 2023
Outros Autores: Reis-Silva, Lilian L. [UNESP], Barretto-de-Souza, Lucas [UNESP], Gomes-de-Souza, Lucas [UNESP], Crestani, Carlos C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.physbeh.2022.114006
http://hdl.handle.net/11449/246204
Resumo: The insular cortex (IC) is engaged in behavioral and physiological responses to emotional stress. Control of physiological functions and behavioral responses has been reported to occur in a site-specific manner along the rostrocaudal axis of the IC. However, a functional topography of the IC regulation of anxiogenic responses caused by stress has never been evaluated. Therefore, we investigated the role of rostrocaudal subregions in the posterior IC in anxiogenic-like effect caused by exposure to acute restraint stress in male rats. For this, rats received bilateral microinjection of the non-selective synaptic inhibitor CoCl2 or vehicle into either the rostral, intermediate or caudal portions of the posterior IC before exposure to acute restraint stress. Then, behavior in the elevated plus maze (EPM) was evaluated immediately after restraint stress. The behavior of non-stressed animals in the EPM was also investigated. We observed that acute restraint stress decreased the exploration of the EPM open arms in animals treated with vehicle in all regions of the posterior IC, thus indicating an anxiogenic-like effect. The avoidance of the EPM open arms was completely inhibited in animals subjected to microinjection of CoCl2 into the intermediate posterior IC. Nevertheless, the same pharmacological treatment into either the rostral or caudal subregions of the posterior IC did not affect the restraint-evoked behavioral changes in the EPM. Taken together, these results suggest that regulation of anxiogenic-like effect to emotional stress along the rostrocaudal axis of the posterior IC might occur in a site-specific manner, indicating a role of the intermediate subregion.
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spelling Evaluation of the posterior insular cortex involvement in anxiogenic response to emotional stress in male rats: Functional topography along the rostrocaudal axisAnxietyInsulaPrefrontal cortexRestraint stressTopographyThe insular cortex (IC) is engaged in behavioral and physiological responses to emotional stress. Control of physiological functions and behavioral responses has been reported to occur in a site-specific manner along the rostrocaudal axis of the IC. However, a functional topography of the IC regulation of anxiogenic responses caused by stress has never been evaluated. Therefore, we investigated the role of rostrocaudal subregions in the posterior IC in anxiogenic-like effect caused by exposure to acute restraint stress in male rats. For this, rats received bilateral microinjection of the non-selective synaptic inhibitor CoCl2 or vehicle into either the rostral, intermediate or caudal portions of the posterior IC before exposure to acute restraint stress. Then, behavior in the elevated plus maze (EPM) was evaluated immediately after restraint stress. The behavior of non-stressed animals in the EPM was also investigated. We observed that acute restraint stress decreased the exploration of the EPM open arms in animals treated with vehicle in all regions of the posterior IC, thus indicating an anxiogenic-like effect. The avoidance of the EPM open arms was completely inhibited in animals subjected to microinjection of CoCl2 into the intermediate posterior IC. Nevertheless, the same pharmacological treatment into either the rostral or caudal subregions of the posterior IC did not affect the restraint-evoked behavioral changes in the EPM. Taken together, these results suggest that regulation of anxiogenic-like effect to emotional stress along the rostrocaudal axis of the posterior IC might occur in a site-specific manner, indicating a role of the intermediate subregion.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Laboratory of Pharmacology São Paulo State University (UNESP) School of Pharmaceutical Sciences, São PauloLaboratory of Pharmacology São Paulo State University (UNESP) School of Pharmaceutical Sciences, São PauloFAPESP: 2019/24478–3CNPq: 431339/2018–0Universidade Estadual Paulista (UNESP)Vitorio, Alex S. [UNESP]Reis-Silva, Lilian L. [UNESP]Barretto-de-Souza, Lucas [UNESP]Gomes-de-Souza, Lucas [UNESP]Crestani, Carlos C. [UNESP]2023-07-29T12:34:33Z2023-07-29T12:34:33Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.physbeh.2022.114006Physiology and Behavior, v. 258.1873-507X0031-9384http://hdl.handle.net/11449/24620410.1016/j.physbeh.2022.1140062-s2.0-85140957489Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhysiology and Behaviorinfo:eu-repo/semantics/openAccess2023-07-29T12:34:33Zoai:repositorio.unesp.br:11449/246204Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:12:56.540005Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of the posterior insular cortex involvement in anxiogenic response to emotional stress in male rats: Functional topography along the rostrocaudal axis
title Evaluation of the posterior insular cortex involvement in anxiogenic response to emotional stress in male rats: Functional topography along the rostrocaudal axis
spellingShingle Evaluation of the posterior insular cortex involvement in anxiogenic response to emotional stress in male rats: Functional topography along the rostrocaudal axis
Vitorio, Alex S. [UNESP]
Anxiety
Insula
Prefrontal cortex
Restraint stress
Topography
title_short Evaluation of the posterior insular cortex involvement in anxiogenic response to emotional stress in male rats: Functional topography along the rostrocaudal axis
title_full Evaluation of the posterior insular cortex involvement in anxiogenic response to emotional stress in male rats: Functional topography along the rostrocaudal axis
title_fullStr Evaluation of the posterior insular cortex involvement in anxiogenic response to emotional stress in male rats: Functional topography along the rostrocaudal axis
title_full_unstemmed Evaluation of the posterior insular cortex involvement in anxiogenic response to emotional stress in male rats: Functional topography along the rostrocaudal axis
title_sort Evaluation of the posterior insular cortex involvement in anxiogenic response to emotional stress in male rats: Functional topography along the rostrocaudal axis
author Vitorio, Alex S. [UNESP]
author_facet Vitorio, Alex S. [UNESP]
Reis-Silva, Lilian L. [UNESP]
Barretto-de-Souza, Lucas [UNESP]
Gomes-de-Souza, Lucas [UNESP]
Crestani, Carlos C. [UNESP]
author_role author
author2 Reis-Silva, Lilian L. [UNESP]
Barretto-de-Souza, Lucas [UNESP]
Gomes-de-Souza, Lucas [UNESP]
Crestani, Carlos C. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Vitorio, Alex S. [UNESP]
Reis-Silva, Lilian L. [UNESP]
Barretto-de-Souza, Lucas [UNESP]
Gomes-de-Souza, Lucas [UNESP]
Crestani, Carlos C. [UNESP]
dc.subject.por.fl_str_mv Anxiety
Insula
Prefrontal cortex
Restraint stress
Topography
topic Anxiety
Insula
Prefrontal cortex
Restraint stress
Topography
description The insular cortex (IC) is engaged in behavioral and physiological responses to emotional stress. Control of physiological functions and behavioral responses has been reported to occur in a site-specific manner along the rostrocaudal axis of the IC. However, a functional topography of the IC regulation of anxiogenic responses caused by stress has never been evaluated. Therefore, we investigated the role of rostrocaudal subregions in the posterior IC in anxiogenic-like effect caused by exposure to acute restraint stress in male rats. For this, rats received bilateral microinjection of the non-selective synaptic inhibitor CoCl2 or vehicle into either the rostral, intermediate or caudal portions of the posterior IC before exposure to acute restraint stress. Then, behavior in the elevated plus maze (EPM) was evaluated immediately after restraint stress. The behavior of non-stressed animals in the EPM was also investigated. We observed that acute restraint stress decreased the exploration of the EPM open arms in animals treated with vehicle in all regions of the posterior IC, thus indicating an anxiogenic-like effect. The avoidance of the EPM open arms was completely inhibited in animals subjected to microinjection of CoCl2 into the intermediate posterior IC. Nevertheless, the same pharmacological treatment into either the rostral or caudal subregions of the posterior IC did not affect the restraint-evoked behavioral changes in the EPM. Taken together, these results suggest that regulation of anxiogenic-like effect to emotional stress along the rostrocaudal axis of the posterior IC might occur in a site-specific manner, indicating a role of the intermediate subregion.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T12:34:33Z
2023-07-29T12:34:33Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.physbeh.2022.114006
Physiology and Behavior, v. 258.
1873-507X
0031-9384
http://hdl.handle.net/11449/246204
10.1016/j.physbeh.2022.114006
2-s2.0-85140957489
url http://dx.doi.org/10.1016/j.physbeh.2022.114006
http://hdl.handle.net/11449/246204
identifier_str_mv Physiology and Behavior, v. 258.
1873-507X
0031-9384
10.1016/j.physbeh.2022.114006
2-s2.0-85140957489
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Physiology and Behavior
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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