Caracteriza��o molecular de Cryptosporidium spp. em cria��es comerciais brasileiras de coelhos

Detalhes bibliográficos
Autor(a) principal: Heker, M. M. [UNESP]
Data de Publicação: 2016
Outros Autores: Nakamura, A. A. [UNESP], Meireles, M. V. [UNESP]
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1678-4162-8836
http://hdl.handle.net/11449/168938
Resumo: Cryptosporidiosis is an important zoonotic disease that can be transmitted via water, food and contact with infected animals and people. Furthermore, clinical and subclinical disease occur in many animal species, including the domestic rabbit. The objective of this study was to determine the occurrence of Cryptosporidium spp., perform its molecular classification and correlate the presence of the parasite to the different animal categories in Brazilian rabbits farms. A total of 514 fecal samples from 21 rabbits farms located in the states of Minas Gerais, Mato Grosso do Sul, Pernambuco, Paran�, Rio de Janeiro, Rio Grande do Sul and S�o Paulo were collected and stored in 5% potassium dichromate. Fecal samples were purified by centrifugal-flotation in Sheather solution and screened for Cryptosporidium spp. oocysts using the negative malachite green staining. Aiming the molecular characterization of Cryptosporidium spp., nested PCR targeting the 18S rRNA gene and gp60 gene followed by sequencing of amplified fragments were accomplished in 55 samples. Eight samples were positive for Cryptosporidium spp. by microscopy (1.56%; 8/514) and seven samples were positive by PCR (12.73%; 7/55). Molecular analysis revealed Cryptosporidium cuniculus for the 18S rRNA gene and C. cuniculus subtype VbA21 for the gp60 gene in kits and does.
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spelling Caracteriza��o molecular de Cryptosporidium spp. em cria��es comerciais brasileiras de coelhosMolecular characterization of cryptosporidium spp. in Brazilian rabbit farmsBrazilCoccidiosisMolecular classificationPolymerase chain reactionRabbitCryptosporidiosis is an important zoonotic disease that can be transmitted via water, food and contact with infected animals and people. Furthermore, clinical and subclinical disease occur in many animal species, including the domestic rabbit. The objective of this study was to determine the occurrence of Cryptosporidium spp., perform its molecular classification and correlate the presence of the parasite to the different animal categories in Brazilian rabbits farms. A total of 514 fecal samples from 21 rabbits farms located in the states of Minas Gerais, Mato Grosso do Sul, Pernambuco, Paran�, Rio de Janeiro, Rio Grande do Sul and S�o Paulo were collected and stored in 5% potassium dichromate. Fecal samples were purified by centrifugal-flotation in Sheather solution and screened for Cryptosporidium spp. oocysts using the negative malachite green staining. Aiming the molecular characterization of Cryptosporidium spp., nested PCR targeting the 18S rRNA gene and gp60 gene followed by sequencing of amplified fragments were accomplished in 55 samples. Eight samples were positive for Cryptosporidium spp. by microscopy (1.56%; 8/514) and seven samples were positive by PCR (12.73%; 7/55). Molecular analysis revealed Cryptosporidium cuniculus for the 18S rRNA gene and C. cuniculus subtype VbA21 for the gp60 gene in kits and does.Universidade Estadual Paulista - UnespUniversidade Estadual Paulista - UnespUniversidade Estadual Paulista (Unesp)Heker, M. M. [UNESP]Nakamura, A. A. [UNESP]Meireles, M. V. [UNESP]2018-12-11T16:43:41Z2018-12-11T16:43:41Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article977-982application/pdfhttp://dx.doi.org/10.1590/1678-4162-8836Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 68, n. 4, p. 977-982, 2016.1678-41620102-0935http://hdl.handle.net/11449/16893810.1590/1678-4162-8836S0102-093520160004009772-s2.0-84984891532S0102-09352016000400977.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporArquivo Brasileiro de Medicina Veterinaria e Zootecnia0,248info:eu-repo/semantics/openAccess2023-10-01T06:08:09Zoai:repositorio.unesp.br:11449/168938Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:41:31.808838Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Caracteriza��o molecular de Cryptosporidium spp. em cria��es comerciais brasileiras de coelhos
Molecular characterization of cryptosporidium spp. in Brazilian rabbit farms
title Caracteriza��o molecular de Cryptosporidium spp. em cria��es comerciais brasileiras de coelhos
spellingShingle Caracteriza��o molecular de Cryptosporidium spp. em cria��es comerciais brasileiras de coelhos
Heker, M. M. [UNESP]
Brazil
Coccidiosis
Molecular classification
Polymerase chain reaction
Rabbit
title_short Caracteriza��o molecular de Cryptosporidium spp. em cria��es comerciais brasileiras de coelhos
title_full Caracteriza��o molecular de Cryptosporidium spp. em cria��es comerciais brasileiras de coelhos
title_fullStr Caracteriza��o molecular de Cryptosporidium spp. em cria��es comerciais brasileiras de coelhos
title_full_unstemmed Caracteriza��o molecular de Cryptosporidium spp. em cria��es comerciais brasileiras de coelhos
title_sort Caracteriza��o molecular de Cryptosporidium spp. em cria��es comerciais brasileiras de coelhos
author Heker, M. M. [UNESP]
author_facet Heker, M. M. [UNESP]
Nakamura, A. A. [UNESP]
Meireles, M. V. [UNESP]
author_role author
author2 Nakamura, A. A. [UNESP]
Meireles, M. V. [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Heker, M. M. [UNESP]
Nakamura, A. A. [UNESP]
Meireles, M. V. [UNESP]
dc.subject.por.fl_str_mv Brazil
Coccidiosis
Molecular classification
Polymerase chain reaction
Rabbit
topic Brazil
Coccidiosis
Molecular classification
Polymerase chain reaction
Rabbit
description Cryptosporidiosis is an important zoonotic disease that can be transmitted via water, food and contact with infected animals and people. Furthermore, clinical and subclinical disease occur in many animal species, including the domestic rabbit. The objective of this study was to determine the occurrence of Cryptosporidium spp., perform its molecular classification and correlate the presence of the parasite to the different animal categories in Brazilian rabbits farms. A total of 514 fecal samples from 21 rabbits farms located in the states of Minas Gerais, Mato Grosso do Sul, Pernambuco, Paran�, Rio de Janeiro, Rio Grande do Sul and S�o Paulo were collected and stored in 5% potassium dichromate. Fecal samples were purified by centrifugal-flotation in Sheather solution and screened for Cryptosporidium spp. oocysts using the negative malachite green staining. Aiming the molecular characterization of Cryptosporidium spp., nested PCR targeting the 18S rRNA gene and gp60 gene followed by sequencing of amplified fragments were accomplished in 55 samples. Eight samples were positive for Cryptosporidium spp. by microscopy (1.56%; 8/514) and seven samples were positive by PCR (12.73%; 7/55). Molecular analysis revealed Cryptosporidium cuniculus for the 18S rRNA gene and C. cuniculus subtype VbA21 for the gp60 gene in kits and does.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01
2018-12-11T16:43:41Z
2018-12-11T16:43:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1678-4162-8836
Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 68, n. 4, p. 977-982, 2016.
1678-4162
0102-0935
http://hdl.handle.net/11449/168938
10.1590/1678-4162-8836
S0102-09352016000400977
2-s2.0-84984891532
S0102-09352016000400977.pdf
url http://dx.doi.org/10.1590/1678-4162-8836
http://hdl.handle.net/11449/168938
identifier_str_mv Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 68, n. 4, p. 977-982, 2016.
1678-4162
0102-0935
10.1590/1678-4162-8836
S0102-09352016000400977
2-s2.0-84984891532
S0102-09352016000400977.pdf
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinaria e Zootecnia
0,248
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 977-982
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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