Detecção do rearranjo da prpoteína BCL2/JH em carcinomas epidermóides de boca e faringe

• Autor(a) principal: Montovani, Jair Cortez [UNESP]
• Data de Publicação: 2010
• Outros Autores: Sales, Magaly Machado, Pardini, Maria Inês de Moura Campos [UNESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/S1809-48722010000300004; http://hdl.handle.net/11449/136961
• topic: Genetics translocation; Molecular biology; Buccal neoplasia; Pharyngal neoplasia; Translocação genética; Biologia molecular; Neoplasias bucais; Neoplasias faríngeas

The BCL2 protein found in the internal mothocondrial membrana regulates the apoptosis preventing the programmed cell death. The translocation (14:18), detected in 70 to 85% of the follicular lymphoma, lead the super expression of BCL2 protein, by juxtaposition of BCL2 gene to the JH segment of the immunoglobulins' heavy chain gene. However, the found of the BCL2 expression in head and neck carcinoma are contradictious. OBJECTIVE: To investigate the presence of the translocation (14:18) of the BCL2 gene in head and neck carcinoma. METHOD: Sixteen DNA samplers were examinated being 13 of squamous cells carcinoma (SCC) and 3 of epidermoid (CE), y means of chain reaction of the polymerase (PCR). RESULTS: The BCL2/JH rearrangement in 2 (15%) of the CCE 13 cases and in none of the 3 cases of CE. The average of the frequency of molecules with rearrangement was 46,44x107. Was not observed association between the rearrangement presence and the exhibition to tobacco and alcohol (p=0, 6545). CONCLUSION: Different from the results found in follicular lymphoma, the presence of the translocation (14; 18) in head and neck carcinomas is not common and, when it occurs, it can be an occasional mutation not associated to exhibition to the tobacco and alcohol.