Siolmatra brasiliensis stem extract ameliorates antioxidant defenses and mitigates glycoxidative stress in mice with high-fat diet-induced obesity

Detalhes bibliográficos
Autor(a) principal: Talpo, Tassiana Cristina [UNESP]
Data de Publicação: 2022
Outros Autores: Motta, Bruno Pereira [UNESP], Oliveira, Juliana Oriel de [UNESP], Figueiredo, Ingrid Delbone [UNESP], Pinheiro, Camila Graça [UNESP], dos Santos, Carlos Henrique Corrêa, Carvalho, Mário Geraldo de, Brunetti, Iguatemy Lourenço [UNESP], Baviera, Amanda Martins [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.orcp.2022.02.004
http://hdl.handle.net/11449/230398
Resumo: Background: Obesity is accompanied by insulin resistance and glucose intolerance, which favor the onset of complications related to oxidative stress. The aim of this study was to investigate the effects and underlying mechanisms of hydroethanolic extract from Siolmatra brasiliensis stems on insulin resistance, glucose intolerance, advanced glycation end product (AGE) formation, and oxidative stress in mice with induced obesity. Methods: C57BL-6 J mice were fed a high-fat diet for 14 weeks and treated with 125 or 250 mg/kg S. brasiliensis extract during the last 7 weeks. The study assessed glucose tolerance and insulin sensitivity, lipid profile, plasma levels of thiobarbituric acid reactive substances (TBARS, biomarkers of oxidative damage), fluorescent AGEs (biomarkers of advanced glycation), and paraoxonase 1 (PON1) activity (antioxidant enzyme). The activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in the liver and kidneys were also investigated. Results: Siolmatra brasiliensis extract had antiobesogenic effects; improved insulin sensitivity and glucose tolerance; decreased the total plasma cholesterol levels; decreased the levels of glycoxidative stress biomarkers, including AGEs (plasma, liver, kidneys) and TBARS (liver, kidneys); and also improved endogenous antioxidant defenses by increasing the activities of PON1 (plasma), SOD (kidneys), CAT (liver, kidneys), and GSH-Px (kidneys). Conclusion: This study expands on our knowledge about the pharmacological properties of S. brasiliensis and substantiates the potential of this plant species to be used as a complementary therapeutic agent to alleviate the metabolic dysfunctions resulting from dyslipidemia and glycoxidative stress.
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spelling Siolmatra brasiliensis stem extract ameliorates antioxidant defenses and mitigates glycoxidative stress in mice with high-fat diet-induced obesityAdvanced glycation end productsAntioxidant defensesCucurbitaceaeLipid peroxidationObesityBackground: Obesity is accompanied by insulin resistance and glucose intolerance, which favor the onset of complications related to oxidative stress. The aim of this study was to investigate the effects and underlying mechanisms of hydroethanolic extract from Siolmatra brasiliensis stems on insulin resistance, glucose intolerance, advanced glycation end product (AGE) formation, and oxidative stress in mice with induced obesity. Methods: C57BL-6 J mice were fed a high-fat diet for 14 weeks and treated with 125 or 250 mg/kg S. brasiliensis extract during the last 7 weeks. The study assessed glucose tolerance and insulin sensitivity, lipid profile, plasma levels of thiobarbituric acid reactive substances (TBARS, biomarkers of oxidative damage), fluorescent AGEs (biomarkers of advanced glycation), and paraoxonase 1 (PON1) activity (antioxidant enzyme). The activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in the liver and kidneys were also investigated. Results: Siolmatra brasiliensis extract had antiobesogenic effects; improved insulin sensitivity and glucose tolerance; decreased the total plasma cholesterol levels; decreased the levels of glycoxidative stress biomarkers, including AGEs (plasma, liver, kidneys) and TBARS (liver, kidneys); and also improved endogenous antioxidant defenses by increasing the activities of PON1 (plasma), SOD (kidneys), CAT (liver, kidneys), and GSH-Px (kidneys). Conclusion: This study expands on our knowledge about the pharmacological properties of S. brasiliensis and substantiates the potential of this plant species to be used as a complementary therapeutic agent to alleviate the metabolic dysfunctions resulting from dyslipidemia and glycoxidative stress.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (Unesp) School of Pharmaceutical Sciences Department of Clinical Analysis, São PauloFederal Rural University of Rio de Janeiro (UFRRJ) Institute of Exact Sciences Department of Chemistry, SeropédicaSão Paulo State University (Unesp) School of Pharmaceutical Sciences Department of Clinical Analysis, São PauloCNPq: 147145/2018-0CNPq: 152992/2019-7CNPq: 305936/2017-4FAPESP: 98/09152-6Universidade Estadual Paulista (UNESP)Institute of Exact SciencesTalpo, Tassiana Cristina [UNESP]Motta, Bruno Pereira [UNESP]Oliveira, Juliana Oriel de [UNESP]Figueiredo, Ingrid Delbone [UNESP]Pinheiro, Camila Graça [UNESP]dos Santos, Carlos Henrique CorrêaCarvalho, Mário Geraldo deBrunetti, Iguatemy Lourenço [UNESP]Baviera, Amanda Martins [UNESP]2022-04-29T08:39:36Z2022-04-29T08:39:36Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.orcp.2022.02.004Obesity Research and Clinical Practice.1878-03181871-403Xhttp://hdl.handle.net/11449/23039810.1016/j.orcp.2022.02.0042-s2.0-85124729201Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengObesity Research and Clinical Practiceinfo:eu-repo/semantics/openAccess2024-06-21T15:19:09Zoai:repositorio.unesp.br:11449/230398Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:34:06.915730Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Siolmatra brasiliensis stem extract ameliorates antioxidant defenses and mitigates glycoxidative stress in mice with high-fat diet-induced obesity
title Siolmatra brasiliensis stem extract ameliorates antioxidant defenses and mitigates glycoxidative stress in mice with high-fat diet-induced obesity
spellingShingle Siolmatra brasiliensis stem extract ameliorates antioxidant defenses and mitigates glycoxidative stress in mice with high-fat diet-induced obesity
Talpo, Tassiana Cristina [UNESP]
Advanced glycation end products
Antioxidant defenses
Cucurbitaceae
Lipid peroxidation
Obesity
title_short Siolmatra brasiliensis stem extract ameliorates antioxidant defenses and mitigates glycoxidative stress in mice with high-fat diet-induced obesity
title_full Siolmatra brasiliensis stem extract ameliorates antioxidant defenses and mitigates glycoxidative stress in mice with high-fat diet-induced obesity
title_fullStr Siolmatra brasiliensis stem extract ameliorates antioxidant defenses and mitigates glycoxidative stress in mice with high-fat diet-induced obesity
title_full_unstemmed Siolmatra brasiliensis stem extract ameliorates antioxidant defenses and mitigates glycoxidative stress in mice with high-fat diet-induced obesity
title_sort Siolmatra brasiliensis stem extract ameliorates antioxidant defenses and mitigates glycoxidative stress in mice with high-fat diet-induced obesity
author Talpo, Tassiana Cristina [UNESP]
author_facet Talpo, Tassiana Cristina [UNESP]
Motta, Bruno Pereira [UNESP]
Oliveira, Juliana Oriel de [UNESP]
Figueiredo, Ingrid Delbone [UNESP]
Pinheiro, Camila Graça [UNESP]
dos Santos, Carlos Henrique Corrêa
Carvalho, Mário Geraldo de
Brunetti, Iguatemy Lourenço [UNESP]
Baviera, Amanda Martins [UNESP]
author_role author
author2 Motta, Bruno Pereira [UNESP]
Oliveira, Juliana Oriel de [UNESP]
Figueiredo, Ingrid Delbone [UNESP]
Pinheiro, Camila Graça [UNESP]
dos Santos, Carlos Henrique Corrêa
Carvalho, Mário Geraldo de
Brunetti, Iguatemy Lourenço [UNESP]
Baviera, Amanda Martins [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Institute of Exact Sciences
dc.contributor.author.fl_str_mv Talpo, Tassiana Cristina [UNESP]
Motta, Bruno Pereira [UNESP]
Oliveira, Juliana Oriel de [UNESP]
Figueiredo, Ingrid Delbone [UNESP]
Pinheiro, Camila Graça [UNESP]
dos Santos, Carlos Henrique Corrêa
Carvalho, Mário Geraldo de
Brunetti, Iguatemy Lourenço [UNESP]
Baviera, Amanda Martins [UNESP]
dc.subject.por.fl_str_mv Advanced glycation end products
Antioxidant defenses
Cucurbitaceae
Lipid peroxidation
Obesity
topic Advanced glycation end products
Antioxidant defenses
Cucurbitaceae
Lipid peroxidation
Obesity
description Background: Obesity is accompanied by insulin resistance and glucose intolerance, which favor the onset of complications related to oxidative stress. The aim of this study was to investigate the effects and underlying mechanisms of hydroethanolic extract from Siolmatra brasiliensis stems on insulin resistance, glucose intolerance, advanced glycation end product (AGE) formation, and oxidative stress in mice with induced obesity. Methods: C57BL-6 J mice were fed a high-fat diet for 14 weeks and treated with 125 or 250 mg/kg S. brasiliensis extract during the last 7 weeks. The study assessed glucose tolerance and insulin sensitivity, lipid profile, plasma levels of thiobarbituric acid reactive substances (TBARS, biomarkers of oxidative damage), fluorescent AGEs (biomarkers of advanced glycation), and paraoxonase 1 (PON1) activity (antioxidant enzyme). The activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in the liver and kidneys were also investigated. Results: Siolmatra brasiliensis extract had antiobesogenic effects; improved insulin sensitivity and glucose tolerance; decreased the total plasma cholesterol levels; decreased the levels of glycoxidative stress biomarkers, including AGEs (plasma, liver, kidneys) and TBARS (liver, kidneys); and also improved endogenous antioxidant defenses by increasing the activities of PON1 (plasma), SOD (kidneys), CAT (liver, kidneys), and GSH-Px (kidneys). Conclusion: This study expands on our knowledge about the pharmacological properties of S. brasiliensis and substantiates the potential of this plant species to be used as a complementary therapeutic agent to alleviate the metabolic dysfunctions resulting from dyslipidemia and glycoxidative stress.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:39:36Z
2022-04-29T08:39:36Z
2022-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.orcp.2022.02.004
Obesity Research and Clinical Practice.
1878-0318
1871-403X
http://hdl.handle.net/11449/230398
10.1016/j.orcp.2022.02.004
2-s2.0-85124729201
url http://dx.doi.org/10.1016/j.orcp.2022.02.004
http://hdl.handle.net/11449/230398
identifier_str_mv Obesity Research and Clinical Practice.
1878-0318
1871-403X
10.1016/j.orcp.2022.02.004
2-s2.0-85124729201
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Obesity Research and Clinical Practice
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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