Streptococcus mutans and actinomyces naeslundii interaction in dual-species biofilm
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/microorganisms8020194 http://hdl.handle.net/11449/201539 |
Resumo: | The study of bacterial interaction between Streptococcus mutans and Actinomyces naeslundii may disclose important features of biofilm interspecies relationships. The aim of this study was to characterize—with an emphasis on biofilm formation and composition and metabolic activity—single- and dual-species biofilms of S. mutans or A. naeslundii, and to use a drip flow reactor (DFR) to evaluate biofilm stress responses to 0.2% chlorhexidine diacetate (CHX). Single-and dual-species biofilms were grown for 24 h. The following factors were evaluated: cell viability, biomass and total proteins in the extracellular matrix, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide—“XTT”—reduction and lactic acid production. To evaluate stress response, biofilms were grown in DFR. Biofilms were treated with CHX or 0.9% sodium chloride (NaCl; control). Biofilms were plated for viability assessment. Confocal laser-scanning microscopy (CLSM) was also performed. Data analysis was carried out at 5% significance level. S. mutans viability and lactic acid production in dual-species biofilms were significantly reduced. S. mutans showed a higher resistance to CHX in dual-species biofilms. Total protein content, biomass and XTT reduction showed no significant differences between single- and dual-species biofilms. CLSM images showed the formation of large clusters in dual-species biofilms. In conclusion, dual-species biofilms reduced S. mutans viability and lactic acid production and increased S. mutans’ resistance to chlorhexidine. |
id |
UNSP_49a9068775d8998bc31b093ee5d9d24e |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/201539 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Streptococcus mutans and actinomyces naeslundii interaction in dual-species biofilmABiofilmDental cariesMicrobial interactionMutansNaeslundiiSVirulence factorThe study of bacterial interaction between Streptococcus mutans and Actinomyces naeslundii may disclose important features of biofilm interspecies relationships. The aim of this study was to characterize—with an emphasis on biofilm formation and composition and metabolic activity—single- and dual-species biofilms of S. mutans or A. naeslundii, and to use a drip flow reactor (DFR) to evaluate biofilm stress responses to 0.2% chlorhexidine diacetate (CHX). Single-and dual-species biofilms were grown for 24 h. The following factors were evaluated: cell viability, biomass and total proteins in the extracellular matrix, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide—“XTT”—reduction and lactic acid production. To evaluate stress response, biofilms were grown in DFR. Biofilms were treated with CHX or 0.9% sodium chloride (NaCl; control). Biofilms were plated for viability assessment. Confocal laser-scanning microscopy (CLSM) was also performed. Data analysis was carried out at 5% significance level. S. mutans viability and lactic acid production in dual-species biofilms were significantly reduced. S. mutans showed a higher resistance to CHX in dual-species biofilms. Total protein content, biomass and XTT reduction showed no significant differences between single- and dual-species biofilms. CLSM images showed the formation of large clusters in dual-species biofilms. In conclusion, dual-species biofilms reduced S. mutans viability and lactic acid production and increased S. mutans’ resistance to chlorhexidine.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)School of Dentistry São Paulo State University (UNESP)São José dos Campos Institute of Science and Technology São Paulo State University (UNESP)Centre for Biofilm Engineering Montana State UniversitySchool of Dentistry São Paulo State University (UNESP)São José dos Campos Institute of Science and Technology São Paulo State University (UNESP)CAPES: 001FAPESP: 2012/17236-4FAPESP: 2013/12326-8FAPESP: 2014/02397-8FAPESP: 2014/16935-1FAPESP: 2015/07679-4CNPq: 304383/2016-3Universidade Estadual Paulista (Unesp)Montana State Universityde Oliveira, Rosa Virginia Dutra [UNESP]Bonafé, Fernanda Salloume Sampaio [UNESP]Spolidorio, Denise Madalena Palomari [UNESP]Koga-ito, Cristiane Yumi [UNESP]de Farias, Aline Leite [UNESP]Kirker, Kelly R.James, Garth A.Brighenti, Fernanda Lourenção [UNESP]2020-12-12T02:35:15Z2020-12-12T02:35:15Z2020-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/microorganisms8020194Microorganisms, v. 8, n. 2, 2020.2076-2607http://hdl.handle.net/11449/20153910.3390/microorganisms80201942-s2.0-85079170393606563636771738265435631614034210000-0002-4470-51710000-0002-2416-2173Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroorganismsinfo:eu-repo/semantics/openAccess2022-03-15T13:29:45Zoai:repositorio.unesp.br:11449/201539Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:01:22.443155Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Streptococcus mutans and actinomyces naeslundii interaction in dual-species biofilm |
title |
Streptococcus mutans and actinomyces naeslundii interaction in dual-species biofilm |
spellingShingle |
Streptococcus mutans and actinomyces naeslundii interaction in dual-species biofilm de Oliveira, Rosa Virginia Dutra [UNESP] A Biofilm Dental caries Microbial interaction Mutans Naeslundii S Virulence factor |
title_short |
Streptococcus mutans and actinomyces naeslundii interaction in dual-species biofilm |
title_full |
Streptococcus mutans and actinomyces naeslundii interaction in dual-species biofilm |
title_fullStr |
Streptococcus mutans and actinomyces naeslundii interaction in dual-species biofilm |
title_full_unstemmed |
Streptococcus mutans and actinomyces naeslundii interaction in dual-species biofilm |
title_sort |
Streptococcus mutans and actinomyces naeslundii interaction in dual-species biofilm |
author |
de Oliveira, Rosa Virginia Dutra [UNESP] |
author_facet |
de Oliveira, Rosa Virginia Dutra [UNESP] Bonafé, Fernanda Salloume Sampaio [UNESP] Spolidorio, Denise Madalena Palomari [UNESP] Koga-ito, Cristiane Yumi [UNESP] de Farias, Aline Leite [UNESP] Kirker, Kelly R. James, Garth A. Brighenti, Fernanda Lourenção [UNESP] |
author_role |
author |
author2 |
Bonafé, Fernanda Salloume Sampaio [UNESP] Spolidorio, Denise Madalena Palomari [UNESP] Koga-ito, Cristiane Yumi [UNESP] de Farias, Aline Leite [UNESP] Kirker, Kelly R. James, Garth A. Brighenti, Fernanda Lourenção [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Montana State University |
dc.contributor.author.fl_str_mv |
de Oliveira, Rosa Virginia Dutra [UNESP] Bonafé, Fernanda Salloume Sampaio [UNESP] Spolidorio, Denise Madalena Palomari [UNESP] Koga-ito, Cristiane Yumi [UNESP] de Farias, Aline Leite [UNESP] Kirker, Kelly R. James, Garth A. Brighenti, Fernanda Lourenção [UNESP] |
dc.subject.por.fl_str_mv |
A Biofilm Dental caries Microbial interaction Mutans Naeslundii S Virulence factor |
topic |
A Biofilm Dental caries Microbial interaction Mutans Naeslundii S Virulence factor |
description |
The study of bacterial interaction between Streptococcus mutans and Actinomyces naeslundii may disclose important features of biofilm interspecies relationships. The aim of this study was to characterize—with an emphasis on biofilm formation and composition and metabolic activity—single- and dual-species biofilms of S. mutans or A. naeslundii, and to use a drip flow reactor (DFR) to evaluate biofilm stress responses to 0.2% chlorhexidine diacetate (CHX). Single-and dual-species biofilms were grown for 24 h. The following factors were evaluated: cell viability, biomass and total proteins in the extracellular matrix, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide—“XTT”—reduction and lactic acid production. To evaluate stress response, biofilms were grown in DFR. Biofilms were treated with CHX or 0.9% sodium chloride (NaCl; control). Biofilms were plated for viability assessment. Confocal laser-scanning microscopy (CLSM) was also performed. Data analysis was carried out at 5% significance level. S. mutans viability and lactic acid production in dual-species biofilms were significantly reduced. S. mutans showed a higher resistance to CHX in dual-species biofilms. Total protein content, biomass and XTT reduction showed no significant differences between single- and dual-species biofilms. CLSM images showed the formation of large clusters in dual-species biofilms. In conclusion, dual-species biofilms reduced S. mutans viability and lactic acid production and increased S. mutans’ resistance to chlorhexidine. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:35:15Z 2020-12-12T02:35:15Z 2020-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/microorganisms8020194 Microorganisms, v. 8, n. 2, 2020. 2076-2607 http://hdl.handle.net/11449/201539 10.3390/microorganisms8020194 2-s2.0-85079170393 6065636367717382 6543563161403421 0000-0002-4470-5171 0000-0002-2416-2173 |
url |
http://dx.doi.org/10.3390/microorganisms8020194 http://hdl.handle.net/11449/201539 |
identifier_str_mv |
Microorganisms, v. 8, n. 2, 2020. 2076-2607 10.3390/microorganisms8020194 2-s2.0-85079170393 6065636367717382 6543563161403421 0000-0002-4470-5171 0000-0002-2416-2173 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microorganisms |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129012577337344 |